ALBUMIN

(BCG Method)
INTENDED USE:
The reagent kit is intended for "in vitro" quantitative determination of
albumin in serum/plasma.
Mix well and incubate for 1 minute at room temperature,
measure the absorbance of the standard (Abs.S) and
CLINICAL SIGNIFICANCE:
sample (Abs.T) against the reagent blank at 630 nm (600 -
Albumin consists of approximately 60% of the total proteins in the
650 nm).
body, the other major part being globulin. It is synthesized in the liver
and maintains the osmotic pressure in blood. Albumin also helps in CALCULATION :
the transportation of drugs, hormones and enzymes. Elevated levels
are rarely seen and are usually associated with dehydration. Albumin Conc. (gm/dl) = Abs. T
Decreased levels are seen in liver diseases (Hepatitis, Cirrhosis). X4
Malnutrition, kidney disorders, increased fluid loss during extensive Abs.S
burns and decreased absorption in gastro-intestinal diseases.
LINEARITY :
The procedure is linear upto 10.0 gm/dl. Samples above this
PRINCIPLE: Concentration should be diluted with saline and results should
Albumin binds with the dye Bromocresol Green in a buffered medium be multiplied by dilution factor
to form a green coloured complex. The intensity of the colour formed
is directly proportional to the amount of albumin present in the NORMAL VALUE :
sample.
Serum : 3.4 - 5.5 gm/dl.
It is recommended that each laboratory establish its
REACTION:
own normal range representing its patient population.
Acidic medium
Albumin + Bromocresol green Green-Albumin BCG Complex
QUALITY CONTROL :
CONTENTS: For accuracy it is necessary to run known controls with every
Reagent 1 : BCG Reagent assay.
Reagent 2 : Albumin Standard, 4 gm/dl
LIMITATION & PRECAUTIONS :
MATERIALS REQUIRED BUT NOT PROVIDED:- 1.Storage conditions as mentioned on the kit to be adhered.
- Clean & Dry Glassware. 2.Do not freeze or expose the reagents to higher temperature
- Laboratory Glass Pipettes or Micropipettes & Tips. as it may affect the performance of the kit.
- Bio-Chemistry Analyzer. 3. Avoid contamination of the reagent during assay process.
4.Use clean glassware free from dust or debris.
SAMPLES: 5.If the reagent is hazy or cloudy do not use it.
Serum, EDTA plasma
Albumin is reported to be stable in the sample for 6 days at 2 - 8°C. BIBLIOGRAPHY :
1.Doumas, B.T. Watson, W.A., Biggs,H.G., Clin, Chem, 1971,
PREPARATION OF REAGENT & STABILITY : 31:87.
All the reagents are ready to use.
The reagent kit is stable at room temperature till the expiry date
mentioned on the kit. CODE NO. PACK SIZE Reagent 1 Reagent 2
The Albumin standard should be stored at 2- 8°C and is stable till Z07 1 x 50 ml 1 x 50 ml 3.0 ml
expiry date mentioned on the label. Z07A 1 x 100 ml 1 x 100 ml 3.0 ml

GENERAL SYSTEM PARAMETERS: IVD

Reaction type : End point UNIONPROJET S.R.L.S.
EC REP Via Aliprandi 41-20851
Lissone (MB)
www.jas-anz.com.au/register

ISO 9001:2015
Wave length : 630 nm (600-650 nm) ISO 13485:2003

Temperature : Room temperature

Incubation : 1 minute
Reagent volume : 1.0 ml
Sample volume : 10 µl
Standard concentration : 4.0 gm/dl
Zero setting : Reagent Blank
Light path : 1 cm

PROCEDURE:
Pipette into clean dry testtubes labeled as Blank (B), Standard (S)
and Test (T) :

Addition sequence (B) (S) (T)

BCG Reagent 1ml 1ml 1ml
Standard - 10 µl -
Sample - - 10 µl