a n r ujuSolu 5 n ur Rr a n Sar il s ruaI u

aar riui 6'ui u u r o r a rl i a r or : ru a r1#r3 n r : rfi o

u,
tl :vTu tri'Lu nr :Ynrgr o-r fl
d u v ad {
i:i fll !! QU U U 1Jfi ? U'l D tY\ I v tu 0 LLA v A :'] aY1A I 5
1:n rrfioo s1erfialfluT: nfrn udofifioun:r ufr u uu:rfitfli n
uriuqn::lrir uiB Rolymerase chai n reaction (PCR)
^a
fraruynqrnuilo Burkholderia (Pseudomonas)
.. d d, da oi r
iir, fl't:tr]:2QLUOUfrUO6SirlUrvriO B. pseudomaLlei
p se u d o ma ller fi s r{l u rur nvr ti u ttn::.ial vr n o1:nlunu [ta v
lufiY:rfr'tl':s
U
ei:uiB
"LurJ:v
#prflunnioudu uvrntuuurT:ndrLralunrodaru frfr fl t"flo
a , & Indirect hemagglutination (lHA)
eu :.r o 1 fl r : lLa fl n r { fl u}l r n sr-r uunl:"i fr o r fl r : a u n : v t1"i^
{mo Lu o
lmmunofluorescent assav (lFA)
lu n : v uua1a fr n ufl i.r i u a r uhj vr n o is'r s ffrJ': u fi fi o r n r :Ta fi n
dr a d u du
9U
6 tJAr.tflovrFlflolJ
LUUilULilUlJV\aUqv:.t O Fl:rdX U r] r Ufi {fi i 40 600/o IO u lavlr v
&-
U
&- Melioidosis - IHA
:?:rnu L:nLt0:{ LlJu tu'll,l'111J aoASlt:o:{ :.Jfl01fl'15:1-Jtt5{
Melioidosis - IFA
rravrfiufiinn:ralu t-2 iu

n'lsnnno
nr : 6 n ri o fr vr r r-i o utriuu ri n r : v r ulq lla vnI: d'l flaTn un : I
-J;3,o^
iir ufr o fr r.l u utl o u lu 6 u uu nv rirlut ru v fr fl u r o tL,.J a r,ri o ufi uri n r :
fr uor r,r r : fi r-l u ld o u * u td, fl , A u ua v #ti": r.l : v tT1 fl h1 u
dr3.jo
:.i :'l u{lu LiFtuxtl n?ta qqFr L1-rfl axttl?ul

fr n riod'ofi ofi nutiun

gt nnaou ta:ulenn-ruli r
ffitr
o1 m8r !8:RC.lUqUr TSOUOOISn

4dd4
Iv:n-rnrr,{

Tu:ar:
o-2589-9l5o,a rio 98463, 98450
O-29 65-97 4.5, O-29 65-97 7 t
ffiffi
zu trt Ft tfl o Q s }.t o 1 fl I ;v n 1 fl ya 1 u Yl { u l 5 rl n t{ a Lal\'l u li1 tta v lD Line: 098-BB1-BB0B
n I: ^3tlj o Ltx\ 5 n : v a 1 u v'"1 vr fl a iu x u
r] o fr'r-l'l u :r-n :r r l"r ! Llvu,l ti 9i? u Emai[ : testkit@dmsc.maiI..go.th
9U

ol nr:td Lr {:'r ufi or nr:tri ri'rtqrnl:orJonlrtluLti{ 1Jr{:r u

fi o r n r : n frr u 1 rir:irul: n o r n r :;{r rirg 6 o 6 n lfl olun :v uua u6 o n

oY 4 q & - vtt a ^ t

].r 11 { rru !
La u u vr a u rua v ri o Ys rir-l': a fr o r n r : uB :r rlu 6': u o r n r :td
nfrr ul:nfr or rfi o?la r uT:a udu an Ytlturfi a :.r'i ta tB u trv'l oa ri
t{ud o pr oo n o-{ #u n'r i er : x t u r t rfr o r il fr ffi nr : 6 r fl n m :r r{r rirg I In
 .a
CSINSUUUSUNOUNENNAOU nlsoluu,a
trlel.ioidosis IHA r{lutprn:roiflqduT:n r:Lfr oouTofr a6":u t. ejufrYutu water bath fr so"c uru 30 urfi iliri

t'tfl 14'r tuoufi r:o 6ri o B. pseudomol lei lufi Yut oI ry'rJr u
fl 'rTfl z. rfirr.Yrur 570 uncoated cetls 225 trt"llu zs pt
J i.,.-
Srqaa'un'!uoufruqutortfr a B. pseudomallei frrnfiorlr.lu inactivated serum
6) yA
lii
d
ril pr
4
rdo n un r o
r,ir Lr,r rn n rJ fr fr 3 u r rn r v n q r.r t o{ tfi Fr LA a n un { fliii :. fr'tt{frqrura4frriosuru
'''i"-''"d^ 3o urfi .' .,i d d d
dv
r ., ,.; v A Centrituge 14; 2000 rpm uru 10u'ryr
I 4.u'rL!1Jun?uLrrio{Centrituge?r2000rpmuru u'rvr^ :.".,""1 ,{au?fl :evL1,1uLx]Ft[o0Fltto{tfl1unax]ttil0ufiuyaxl
,

ff r:.r r : n il o.i uiurir un r r*i rir-l': uT:n rufr o o uTn fr ao vfr : vriu i 4. u'rL!!1-Jn?uLrrio.i 10 ' ,, ^ .4- ts_ 4__ ^ _ ^-,1,_ ^: ^
I

, q (absorbed 4 -.u,o tJaau :QvLTULX.lFlLfi0FlttO{:?l]0UGr:{fl41{YA:]

uau6!o fr qir rv\r va{ n"j'"r rnrusi%or nul n fr 5. 1-J'lEl.JUL'61
ri ). urA?uLA \aDSOrDeO Serum taoal{
serum t{el{ l.I 1:1U) Lljilrnri
1:lU/ LUvl lill).. , ', e
qr
', , I
,"...i
o'ruzuatnrnoiasao frrfrolfrfr3urrnrrnrilrolufin
"n^,^", 16oortor > 5oo/o

, . li.,i,',,li..,.iiii1,i,,ji1.[.1, Serum control uiay Cel.[ control 6'0.1"[ri'r.raal

56nnaou
nEltijauja
t. u6oo'r.i absorbed serum r{lu two fold ditution 6'ru
diluent bulfer u3ltln 1.:1.0,I:20,1:40, 1:80, 1:160, e:r^..i^.^
eJ:XltOUl : IHA Liter > ^.^ -a^.Ft- - -n,- ---a-.--u-i,-
160 6loLuutnruclLUfll:?UAflUL:Fl
"
pn:rril6'l"Lu
1.:320,1.:640, 1.'.1.280, 1.:2560,
I.)ZV, -L.O+U, l.lZOV, L.ZJOV, L:51.20
L.J IZV vl ,^,^^^-,r^^-
lJ.J6l lvl UbLJ I .
" du ^
:Ttj'tT1
Microtiter ol.ate vaufi 1-10 rjirrn:vnrnv
e 25 ut.
I ; s d, a
st:tJo : ?Jrx.l11fio{}.1 lil-ertv\x.JfluL1']uatyrto{c,:1]tL:n
". :". u,
(r,rqrqnri'r
\rl5l&blvl vt tQbVtYtYt r
ulri'q nfrvl\ 25
zJ pl.)
Pt/ lifiiiilll
, ..,i
Y q du ! a
'
2. vuodrur Melioidosis LesL fiYl fru6o
cetls a.itia]rlu
af iarflva].rfi t firr.rq:rfi to vrarav 25 trrl finat nulutnn
. .. 1 I

di lution rvir ril 1.:20, !:40, l" :80, ...... tO24O n rlrir d'l
Fl x r:rtx ua v o': ril sir uv! r v uvi I rir: 82. 6. 9 6.2 o/o n rl eir nil
:. raqrfi t2 rflu cetl control (CC)l?i diluent iuffer 25 A - -', s
o'

u Lllo?1oaolnuzutt':ut:nmfroouTerfra
u9' 70 :ru 6orfloqafiv\
Dituent buffer 1 flxn ptl. uau drul Mel.ioidosis lest ceU.s 25 ;.rI
6u 55 :ru Lrav auln6 54 t'rE.r
Aa,
Mel.ioidosis test ce[s 1 t]?fl q. r,ra:rfr 11 rtlu serum control (SC) lrirjrsr Negative
c
ufrrrirurl{fr 4 - 10"c evFr{anrvllnnnir t fl
Negative control cetts 1 tlxn control cetls rtav absorbed serum 1:10 odxav 25 prl.
rl?oul{
5 % uncoated celts 1 t?Ft 5. unrv dx1 Microtiter plate turl tfiazuauar:l#ldrriu du i
6u:ilLFIU?
a-

- X,qq , ja /1'::iA Ur{flU l-2 dlFltn

qt

Positive control serum 1 l'ta 6. n { L'r Lunaorvnrer:r:rdufi o

9{
ruilfl fr #o { uru 2 rY:hr
Negatlve'control serum 1 txo 7. lir Positive controI serum uunv Negative controI
ru ,o,
serum n'tLnU9L!nun15iloaaLfl 'laul{9? uvtr
.ror#o -