Lab 1

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Dallas College

ENVR 1401 Online

Scientific Method
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Objectives
1. Students will explain the steps of the scientific method.
2. Students will explain what distinguishes a good scientific hypothesis.
3. Students will define, identify, & give examples of dependent and independent variables.
4. Students will explain the difference between qualitative and quantitative data.
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Please Highlight or type answers in a different color.

Information
Researchers throughout the world use a standardized technique of answering scientific questions. This technique is called
the scientific method. You will use the scientific method throughout this course to critically investigate your
environment. There are seven steps to the scientific method:

Observation
-As you investigate your environment, you will observe many interesting phenomena.
Question
-You will immediately begin to ask questions about what you observe.
Hypothesis
-Your question can be turned into a hypothesis which is an educated, yet untested answer to the question.
-Your hypothesis must be testable and falsifiable. This means we must be able to design an experiment to test the
validity of your hypothesis. If your hypothesis is not true, your results should reject your hypothesis.
-What would happen if your hypothesis is not correct? That is your null hypothesis.
Experiment
-To test your hypothesis, you must design an experiment. An experiment attempts to support or disprove a
hypothesis.
-One mistake can ruin your results. To ensure your results are correct, be careful when collecting data from your
experiment.
Results/Analysis
-You will need to closely examine data you collect to interpret the results of the experiment. You may have to
perform your experiment multiple times to increase your certainty of the results.
Conclusion
-After analysis of your data, can you support or reject your hypothesis? No results can prove a hypothesis.
-If the results of your experiment neither supports nor rejects your hypothesis, you may need to modify your
hypothesis.
-Explain the data. This is where the lecture material is tied into the laboratory results.

Activity 1: Hypothesis
After deciding on a question to test, the first thing to do is to propose a possible explanation of the phenomenon or an
answer to the question. This is the hypothesis or a statement of change. It is basically an educated guess of what you
think will occur during the experiment. In most studies, there is also an alternative null hypothesis (a statement of no
change). The null hypothesis is written the same as the hypothesis with the addition of the word “not”. For example the
hypothesis “Plants grow taller with fertilizer.” would have a null hypothesis of “Plants do NOT grow taller with fertilizer.”
If you were to change any other aspect of the wording it would be considered a completely different hypothesis.

It is very important that the hypothesis is scientifically testable and potentially falsifiable. For example, the hypothesis:
“Monet is the greatest painter of all time” is not scientifically testable since it is a subjective statement or an opinion.
However, the hypothesis could be changed to make it testable, for example: “Monet’s paintings are the most valued based
on auction prices.”
Exercise 1: Hypotheses
a. List one example of a falsifiable scientific hypothesis about Oreo cookies (1 point)
Oreo cookies help us stay healthy.
b. State the null hypothesis about Oreo cookies in relation to your hypothesis above (1 point)
Oreos do not improve our health.
c. List one example of a hypothesis about Oreo cookies that cannot be tested scientifically AND explain why it
cannot be tested scientifically (1 point)
Oreos can improve memory.
Activity 2: Variables
An experiment is an investigation conducted to test a hypothesis. To do this, scientists design experiments so that
alterations to one variable cause another variable to change in a predictable way. A variable is any factor, trait, or
condition that can exist as different types or in differing amounts. An experiment usually has three kinds of variables:
independent, dependent, and controlled.
Designing experiments to test hypotheses requires considerable thought. The variables must be identified, appropriate
measures developed, and influences outside of the experimental variables must be controlled. The independent variable
will be varied during the experiment, while the dependent variable should change as a result. It “depends” on the
independent variable. Controlled variables or constants are also identified and maintained throughout the experiment.
If kept constant, the controlled variables should not cause changes in the dependent variable which would confuse the
results of the experiment. Many experiments compare the results of the experimental treatments to a control treatment,
in which all experimental variables are removed.

For example, if different fertilizers were being tested to see their effect on plant growth, the independent variable (the one
altered) would be the different type of fertilizers. The dependent variable would be the resulting plant height. To make
sure that the fertilizer type was the only factor determining plant growth, everything else would have to be constant or the
same for each plant. This would include using the same plant in each pot, the amount of light and water each plant
received, the location of the plants, the amount & type of soil used to grow the plants and the list goes on. These are the
controlled or constant variables. Finally, to see how the plant grows naturally without any influence one plant would
have to be grown without any fertilizer at all. This would be the control treatment that will be used to compare to the
experimental treatment (fertilizer types).

Exercise 2: List 10 independent variables that can affect seed germination (10 points).
a. Water
b. Oxygen
c. Temperature
d. Light
e. Seed dormancy
f. Darkness
g. pH of soil
h. Soil moisture
i. Burial depth of seed in soil
j. Level of salt in soil
Activity 3: Experiment
The control group in an experiment is the group who does not receive any treatment and is used as a benchmark against
which other test results are measured. A control group is used in an experiment as a point of comparison, so that it can be
isolated in testing that the experiment did, or did not, have the intended effect.
For example, scientists have developed many tests using reagents (substances used to detect another substance) to identify
different biomolecules. In order to perform these tests correctly, two different treatments must be completed to provide
standards for comparison. The first treatment is called a positive control and contains the variable being tested. The
second treatment is the negative control, which does not contain the variable being tested. It is there to make sure your
testing chemicals are not contaminated and to see what a negative reaction to the test will look like.

Exercise 3: Identify the parts of this experiment: A researcher wants to find out if spraying fruits and vegetables with
pesticides affects the vitamin levels in those foods.

a) the independent variable (1 point): Level of pesticide sprayed

b) the dependent variable (1 point): Vitamin Levels

c) two constants (1 point): Temperature and water

d) the control treatment (1 point): Fruits and vegetables without pesticide treatment

Activity 4: Data
Data are reported in objective terms that allow for independent interpretation by anyone reading the report. The preferred
method of reporting data is the presentation of results in tables and graphs that provide a quick and clear overview of any
observed effects. There are two types of data: qualitative and quantitative.

Qualitative data include qualities such as color, smell and taste. These are subjectively perceived and can be difficult to
express in an objective manner. While everyone conducting the experiment may agree that the solution changed color,
there may be variation in what individuals identify as blue, light blue, etc. (I like to think of the “L” in quaLitative
standing for “Looks” or “quaLity”.)
Quantitative data include qualities that can be measured objectively such as weight, volume, length and temperature.
Quantitative data have a number associated with them and can be reported in universally accepted measurable units. This
makes it easy for others to interpret the results. (Think of the “N” in quantitative standing for Number.)
Both types of data are valid and important. In some instances an experiment may result in just one or the other type of
information. Frequently, the investigator may collect and report both types of data.

Exercise 4: Label the following as qualitative (L) or quantitative (N) (10 points).

Temperature of 23 °C N
Increase in color intensity L
pH values N
Bitter taste L
Sound increase L
Measurement of nitrates N
Number of deaths L
Precipitation N
Habitat type L
Level of diversity N

Activity 5: Results/Analysis
To understand data better, it is usually shown in some type of graphical format. The same data can be portrayed in
different formats depending upon what is being emphasized. A pie chart shows the proportions of different categories
(qualitative data). The amount of the pie (circle) correlates to the percentage. It can be as simple as just two categories or
it can contain several categories. The more categories a chart has, the more difficult it is to read. If there are many
categories that have small numbers they are frequently lumped into an “Other” category. A bar chart is used to show
comparisons between data. The independent variable is found on the x-axis while the dependent variable is put on the y-
axis. Finally, the line graph is used to show data changes over time. Once the points are plotted a best fit or trend line
is drawn to show a smooth representation of the data. It is not necessary to connect each dot. The axis labeling is the
same as it is for bar charts.

Scatter graph showing a best fit line.

Exercise 5a: Creating a complex pie chart. Create a pie chart for ONE characteristic with at least four categories but not
more than six. Choose a characteristic that varies among students. Appropriate examples include shoe type, favorite
subject, mobile phone service, OR type of car owned (only pick one topic/characteristic with multiple categories –
e.g., favorite mineral is the topic/characteristic and the categories would be feldspar, quartz, diamond, ruby, or
sapphire – don’t use this example). Poll friends and family to gather data and create a pie chart with your data – don’t
forget to include labels (5 points)

Graph Title: Shoe brands

Category Number of People Percent of People

Nike 14 35%
Adidas 10 25%
Puma 6 15%
Converse 4 10%
Under armour 4 10%
Fila 2 5%
Discuss the graph (highest/lowest, trends, what the colors/axes mean, etc.) (1.5 points):

Exercise 5b: Creating a bar chart. The information in a pie chart can also be shown as a bar chart. The y- axis on the bar
chart can be percentages or any quantitative variable. Bar charts must always have both axes labeled and a figure
description given. Create a bar chart for the same data you collected in exercise 5a (both graphs should represent the
table) (5 points). (Feel free to delete given graph paper & insert personally created graph.)
Discuss the data the graph shows (1.5 points):
The data shown in the graphs above is the amount and percentage of people who prefer different shoe brands over
the other and consider them their favorite.

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