Integrated Autosampler (I-AS) : Agilent ICP-MS

Agilent ICP-MS
Integrated Autosampler (I-AS)

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Manual Part Number Warranty Safety Notices
G3160-90027 Revision A The material contained in this document
October 2018 is provided “as is,” and is subject to being
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Content
1 Integrated Autosampler Overview and Configuration 5

Overview 6
Features 8
Operations Overview 8
Rinse System 9
System Configuration 10
Main Unit Components 10
Specifications 15
Main Unit 15
External Bottle 15
Single Sample Position 15
Utility Requirements 16
Electromagnetic Compatibility 17
Operating Environment 17
Symbols Used on this Instrument 18
Serial Number 18
Safety Information 19
2 Setup 21
Unpacking the I-AS 22
Setting up the I-AS 24
Preparation of the Arm 24
Installing the Support Table or Bracket 26
Tubing and Wiring 27
Installing the Sample Tray 32
Installing the Tray Cover 33
Single Sample Position Setup 34
Software Settings 35
G7201C (ICP-MS MassHunter 4.1 or later) Configuration 35
G7201A/B Configuration 38
Integrated Autosampler (I-AS) 3

Content
G7200B Configuration 42
Settings 44
Checking and Adjusting Operations 46
Checking Operations 46
Installing and Adjusting the Needle 53
3 Operation 63
Measuring with Single Sample Position
(Using the G7200B ICP-MS Software) 64
Measuring with Single Sample Position 64
Single Sample Position Run Dialog Box 65
4 Maintenance 67
Daily Maintenance 68
Power Cable Fuse 69
4 Integrated Autosampler (I-AS)

1 Integrated Autosampler Overview and
Configuration
Overview 6
Features 8
Operations Overview 8
Rinse System 9
System Configuration 10
Main Unit Components 10
Specifications 15
Main Unit 15
External Bottle 15
Single Sample Position 15
Utility Requirements 16
Electromagnetic Compatibility 17
Operating Environment 17
Operating Conditions 17
Storage Conditions 17
Symbols Used on this Instrument 18
Serial Number 18
Safety Information 19
The Integrated Autosampler (I-AS) is a very flexible automated sample

introduction system capable of handling multiple sample vials. The I-AS is a
dedicated peripheral for the Agilent ICP-MS, designed to optimize instrument
capability.
This chapter describes the features, configuration and specifications of the I-AS.

1 Integrated Autosampler Overview and Configuration
Overview
Overview
The I-AS is designed to automate analysis and improve productivity for chemical
research and industrial testing facilities.
The I-AS has six types of sample trays. Replacing the sample tray allows the
instrument to handle sample vials of different sizes. Also, using the Single
Sample Position feature enables the use of a single bottle for analysis.
OPM070-2
Figure 1. Agilent 7800/7900/8900 ICP-MS equipped with the I-AS

Overview
OPM070
Figure 2. Agilent 8800/7700 ICP-MS equipped with the I-AS

Features
Features
Equipped with the I-AS, the ICP-MS is able to introduce a sample from a vial on
the I-AS sample tray, or introduce a sample directly from Single sample position.
The I-AS is equipped with a rinse port and an external bottle for cleaning the
needle. Depending on the option selected, up to three external bottles can be
used.
Needle
Three external
bottles
Tray cover
Rinse port
Sample tray Main unit
Figure 3. I-AS
Operations Overview
I-AS operation is controlled by MassHunter Workstation.

The I-AS uses command settings from MassHunter Workstation to rotate the
sample tray and select a sample vial, rotate the arm and move the needle up or
down, and remove the sample from the needle.
To prevent sample contamination, the needle tip is cleaned in the overflow rinse
port and from the external bottles when needed.

Rinse System
Rinse System
The overflow rinse system (rinse port) uses the peristaltic pump for rinse and
waste fluids.
The internal diameter of the peristaltic pump tubing outlet is larger than inlet.
This prevents rinse fluids from overflowing from the rinse port.

System Configuration
System Configuration
Main Unit Components
In addition to the sample tray driver and other peripheral equipment, the main
unit is equipped with a needle, arm, and a rinse port for cleaning the needle.
The I-AS is mounted on the ICP-MS and connected to MassHunter Workstation.
The sample tray mounts onto the I-AS.
Arm
Needle
Tray cover
Three external
Sample tray
bottles
Power display LED

Rinse port
Main unit
Reset switch
Figure 4. I-AS Components

Needle
The needle is installed on the arm of the main unit. The needle tube is connected
to the sample introduction tube.
Arm
The needle is installed on the arm. The arm can rotate and move up and down to
position the needle in the necessary location.
Reset switch
On the right side of the main unit is a red reset switch. Push this switch to
interrupt or stop the instrument in an emergency situation.
Pushing the reset switch will cancel Autosampler operation, return the needle to
home position, and initialize the I-AS. It will also cancel any analyses in process.
Rinse Port
The rinse port uses an overflow feature to supply rinsing fluid to clean the tip of
the needle.
External Bottle
In addition to cleaning with the rinse port, the external bottle is used when
cleaning with other types of cleaning fluids. Up to three external bottles can be
used. These may also be filled with standard tuning solution.
Power Display LED

The LED illuminates when the I-AS is powered ON.

Sample Tray
The sample tray contains slots for holding sample vials. Insert a sample vial into
a slot on the sample tray.
The following six types of sample trays are available as options. Select the
sample tray that matches the sample vials to be used.
Table 1 Sample Tray Types
Sample Tray Max. No. of Vials Sample Vial Specifications
Type A 89 6 mL
Type B 53 18 mL
Type C 18 50 mL
Type D 89 1.5 mL or 2 mL, 18 mL
Type E 89 6 mL, 18 mL
Type F 15 Used for 48 (OD) × 55 (H) mm vial
Figure 5. Sample Tray Types (Photo Shows Type A)

Single Sample Position

The Single Sample Position allows the user to do a single sample. To use this
function, place the bottle containing the sample in the Single Sample Position.
Bottle for Single

Sample Position
Single Sample
Position stand
Figure 6. Introducing a sample with Single Sample Position
Power switch
Power cable
connector
Communications network connector

Figure 7. Rear side of I-AS main unit

Power Switch
I-AS power switch.
Power Cable Connector

This connects the power cable.
Communications Network Connector

This part connects to the communications network cable.
Rinse Fluid Inlet

Tube Connector Rinse Fluid Outlet
Tube Connector
Figure 8.
Rinse Fluid Inlet Tube Connector

This is labelled “IN” on the instrument. This is the clean fluid inlet for the rinse
port and connects to the rinse fluid inlet tube.
Rinse Fluid Outlet Tube Connector

This is labelled “OUT” on the instrument. This is the waste outlet for the rinse port
and connects to the rinse fluid outlet tube.

Specifications
Specifications
The following specifications are subject to change without notice.
Main Unit
Dimensions: 290 (W) x 360 (D) x 220 (280, probe included) (H) mm
Weight: 5 kg
External Bottle
Dimensions: 56 (Diameter) x 61 (H) mm
Single Sample Position
The client must provide this bottle. Bottles up to 100 mL may be used. The
allowable bottle dimensions are shown below.
Dimensions: A diameter of 55 mm or less and a height of: 126 mm or less.

Utility Requirements
Utility Requirements
The following utilities are required to operate the I-AS:

Electrical Power: 100-120 V AC, 50/60 Hz, 1 A
200-240 V AC, 50/60 Hz, 0.5 A
Overvoltage Category: II (see Note)
Pollution Degree: 2 (see Note)
Fuses: Main fuses in appliance inlet (see Figure 57)
2 x T 1AL/250 V
Secondary fuses in DC 5 V and DC 24 V line

(inside the Autosampler, left of the power supply)
F1: T 2 AL/250 V
F2: T 2 AL/250 V

Electromagnetic Compatibility
Electromagnetic Compatibility
This device complies with the requirements of EN550011, Group 1, Class B.
EN55011
Group 1 ISM equipment: Group 1 contains all ISM equipment in which there is
intentionally generated and/or used conductively coupled radio-frequency energy
which is necessary for the internal functioning of the equipment itself.
Class B equipment is equipment suitable for use in domestic establishments and
in establishments directly connected to a low voltage power supply network
which supplies buildings used for domestic purposes.
ICES/NMB-001
This ISM device complies with Canadian ICES-001
Cet appareil ISM est conforme à la norme NMB-001 du Canada.
The “Installation category” implies the regulation for impulse withstand voltage. It
NOTE is also called the “Over voltage category.” “II” applies to electrical equipment.
“Pollution degree” describes the degree to which a solid, liquid, or gas which
deteriorates dielectric strength is adhering. “2” applies to a normal indoor
atmosphere.
Operating Environment
Use the I-AS only under the following operating conditions:
Operating Conditions
Ambient temperature: 5 - 35 °C (41 - 95 °F)
Relevant humidity: 20 - 80 % (no condensation)
Storage Conditions
Ambient temperature: -35 - 65 °C (-31 - 149 °F)
Relevant humidity: 15 - 85 %

Symbols Used on this Instrument
Symbols Used on this Instrument
The following symbols are used on this instrument:
To avoid personal injury, death, or damage to the instrument, the operator must refer to an
explanation in the Manual.
Protective grounding terminal.
Alternating current.
ON (power)
OFF (power)
Serial Number
The Integrated Autosampler is identified with a serial number on a label on the

instrument.
When corresponding with Agilent about your Integrated Autosampler, be sure to
include the model type and the full 15-digit serial number.

Safety Information
Safety Information
The I-AS has been designed and tested in accordance with IEC publication
1010-1, Safety Requirements for Electrical Equipment for Measurement, Control,
and Laboratory Use.
Only connect the I-AS to a power source with a protective earth contact. Use
WARNING only a certificated power cord to make a connection. Connecting the I-AS to a
power source that is not equipped with a protective earth contact creates a
shock hazard for the operator and can damage the instrument. Likewise,
interrupting the protective conductor inside or outside the I-AS or defeating the
power cord ground creates a shock hazard for the operator and can damage
the instrument.
• Do not use organic solvents and other combustible or flammables in the

WARNING rinse port fluid. Combustibles or flammables can ignite during use. Also,
some organic solvents can damage the internal elements of the instrument.
• When the external bottle contains combustible or flammable fluid, make
sure the area is well ventilated. If the area is permeated with flammable or
combustible gas, it could ignite.
If an overflow of the rinse receptacle caused by blockage of the peristaltic

WARNING pump or a liquid spillage is noted, switch off the sampler and unplug the main
cords. The sampler then should be cleaned and wiped dry.
Never use the I-AS in a way not described in the manual. Improper use may
WARNING result in danger to the user.
If rinse fluid, sample fluid, standard fluid or other liquids spill, wipe up
WARNING immediately. These liquids can short circuit or corrode the instruments. Highly
alkaline or acidic fluids can injure the skin and mucus membranes. Exercise
caution when handling these fluids.

Safety Information
Do not cover the air circulation outlet holes on the right side of the I-AS.
CAUTION
Precautions when moving the I-AS are listed below:

CAUTION
• Make sure the I-AS Power switch is turned OFF.
• Make sure all cables between other units are unplugged and the utility
tubing is disconnected before moving equipment (small adjustments can be
made without unplugging and disconnecting).
• When shipping the I-AS, secure the arm prior to shipping.
Do not remove or install the tray on to the I-AS while the needle is moving. The
CAUTION I-AS can be damaged if the needle is touched during operation.
If you change the tray type used, you must change the tray type in the I-AS
Configuration dialog box.
Verify the following before beginning measurements with the I-AS:

CAUTION
• Is the rinse port waste bottle empty?
• Are the rinse fluid, external bottles, and sample vials ready, and does each
bottle contain the required amount of fluid prior to measuring?
• Do not use organic solvents and other combustible or flammables in the

WARNING rinse port fluid. Combustibles or flammables can ignite during use. Also,
some organic solvents can damage the internal elements of the instrument.
• When the external bottle contains combustible or flammable fluid, make
sure the area is well ventilated. If the area is permeated with flammable or
combustible gas, it could ignite.
• Always make sure there is at least 10mm or more distance between the
CAUTION surface of the sample fluid to the top edge of the vial. This is to keep the
sample fluid lower than the rinse port fluid, and ensure the entire needle is
thoroughly cleaned.
• If rinse fluid, sample fluid, standard fluid or other liquids spill, wipe up
immediately. These liquids can short circuit or corrode the instruments.
Highly alkaline or acidic fluids can injure the skin and mucus membranes.
Be very careful when handling these fluids.

2 Setup
Unpacking the I-AS 22
Setting up the I-AS 24

Preparation of the Arm 24
Installing the Support Table or Bracket 26
Tubing and Wiring 27
Installing the Sample Tray 32
Installing the Tray Cover 33
Single Sample Position Setup 34
Software Settings 35
G7201C (ICP-MS MassHunter 4.1 or later) Configuration 35
G7201A/B Configuration 38
G7200B Configuration 42
Settings 44
Checking and Adjusting Operations 46

Checking Operations 46
Installing and Adjusting the Needle 53

2 Setup
Unpacking the I-AS
Unpacking the I-AS
The I-AS arrives packaged in multiple boxes. Below is a packing list of the
contents. Check to make sure all the necessary components are included.
The number of sample vials and sample trays will differ depending on the option
ordered.
Be careful not to drop or hit the components against anything when removing
CAUTION them from their packaging. Many parts are heavy or can be easily damaged.
Table 2 I-AS Packaging Contents
Name Quantity
Main Unit 1
Tray Cover 1
Table 3 Peripheral Equipment Packaging Contents
Name Quantity
Bracket with Screws 1
Needle 1
Rinse Fluid Inlet Tube 1
Rinse Fluid Outlet Tube 1
Power Cable 1
Communications Network Cable 1
External Bottle(s) with cover(s) 3
External Bottle(s) with Slotted Cover(s) 3
Tube Clip(s) 2

2 Setup
Unpacking the I-AS
Table 4 Sample Tray Packaging Contents
Name Vial Types
Type A 6 mL
Type B 18 mL
Type C 50 mL
Type D 1.5 mL or 2 mL, 18 mL
Type E 6 mL, 18 mL
Type F 55 (OD) × 48 (H) mm vial
50 mL Vial
18 mL Vial
6 mL Vial
2 mL Vial
1.5 mL Vial
The number of boxes will differ depending on the options ordered. Make sure to
NOTE verify that all the necessary components are present.

2 Setup
Setting up the I-AS
Setting up the I-AS
The following describes how to install the I-AS.
Preparation of the Arm
• Do not work on the underside of the instrument alone. Turning the

CAUTION instrument upside down can deform or damage the arm.
• After removing the arm shipping screw, store it for future use. The base of
the main unit is equipped with a shipping screw storage hole.
• When shipping the I-AS, secure the arm prior to shipping.
1 Remove the arm screw.

2 Store the screw.
Store the screw
Remove the arm

shipping screw
Figure 9.

2 Setup
Preparation of the Arm
3 Loosen the two fixed arm block screws and slide the arm block with screws to
the right.
Loose these two screws and

slide to the right
Figure 10.
Do not remove the two fixed arm block screws. This will cause the arm block to
CAUTION be removed from the bracket.
4 Secure the screws after sliding to the right.
Secure these two

screws
Figure 11.

2 Setup
Installing the Support Table or Bracket
Installing the Support Table or Bracket
For 7800/7900/8900, place the support table next to ICP- MS as shown in

Figure 12. And for 8800/7700, install the bracket to ICP- MS as shown in
Figure 13.
Sample Introduction Tray

Support Table
OPM075-2
Figure 12. Installing to 7800/7900/8900

2 Setup
Tubing and Wiring
Screw
Screw
Screw
Sample Introduction Tray
Support Bracket
Screw Leg
OPM075
Figure 13. Installing to 8800/7700
Tubing and Wiring
1 To install the tubing and wiring, place the I-AS on the bracket installed on the
ICP-MS with its power display LED facing front.
2 Connect the Peristaltic Pump inlet (sample uptake) and outlet (drain) tubes.
Connect the rinse fluid inlet and outlet tubes to the IN and OUT ports. Tighten
the connectors by hand.
Do not connect sample tubing for Outlet and drain tubing for Inlet. The internal
NOTE diameter of the drain tubing is larger than the sample tubing to prevent rinse fluid
from overflowing.
When sample tubing is connected to Outlet and drain tubing is connected to Inlet,
rinse fluid could overflow from the rinse port.

2 Setup
Tubing and Wiring
Rinse fluid outlet tube

Rinse fluid inlet tube
Figure 14.
Do not touch the Peristaltic Pump while it is rotating.

CAUTION
3 Connect the communications cable.
Communications
cable
Figure 15.

2 Setup
Tubing and Wiring
4 Connect the communications cable to the PC.

The connection cable differs between G7201A/B/C and G7200B ICP-MS
software.
• G7201A/B/C Connection
Connect the other end of the communications cable to the serial-USB
converter cable, then connect to the PC USB port.
Connect to PC USB port
Serial-USB Converter Cable
Figure 16. Serial-USB converter cable
• G7200B Connection
Connect the other end of the communications network cable to the PC
RS-232C connector.
When ISIS is used with I-AS, connect the communication network cable to
the ISIS controller (COM1).
5 Make sure the I-AS power switch is in the OFF position.
Make sure to check the following prior to connecting the power cable. Skipping
CAUTION this procedure can result in damage to the instrument.
• Before connecting the power cable, make sure it is designated for this
purpose.
• Make sure the protective grounding is secure.
• Only use the provided cables and properly connect them in their designated
locations. Do not use unauthorized cords or cables. Do not uses these
cables on instruments other than those listed in this manual.

2 Setup
Tubing and Wiring
6 Connect the power cable to the connector. The connector is designed to be

inserted in only one direction.
Power cable
Figure 17.
7 Connect the other end of the power cable to a properly grounded wall outlet.
Leave the I-AS power switch in the OFF position.
In the case of leakage (liquid under the I-AS), disconnect the power cable and
WARNING contact your local Agilent office.

2 Setup
Tubing and Wiring
8 Connect the rinse fluid outlet tube to the rinse port waste bottle.
Use the tube clip to secure the tube to the bottle.
Tube
Tube clip
Bottle
Figure 18.
9 Connect the rinse fluid inlet tube to the rinse port rinse fluid bottle.
Use the tube clip to secure the tube to the bottle.

2 Setup
Installing the Sample Tray
Installing the Sample Tray
1 Slide the sample tray onto the shaft of the main unit and set into place.
Line up the sample tray mating pin with the mating grove on the shaft. After
inserting the sample tray, rotate it slightly until the pin and groove line
interlock.
Sample tray underside
Mating pin
Mating groove
Figure 19.
Sample tray
Main unit
Figure 20.

2 Setup
Installing the Tray Cover
Installing the Tray Cover
1 Place the tray cover over the sample tray.

Line up the groove on the tray cover with the projections on the main unit and
place the cover in position.
Tray cover groove
Projection on main unit
Figure 21.
Tray cover
Figure 22.
The tray cover is acrylic. Organic solvents and some sample materials may
CAUTION cause the cover to become discolored.

2 Setup
Single Sample Position Setup
Single Sample Position Setup
Use the Single Sample Position when sequencing a single sample.
Use the Single Sample Position with the sample tray removed from the
NOTE instrument.
The Single Sample Position sits in the designated stand. Make sure the mouth of
the bottle is centered on the Single Sample Position stand.
The Single Sample Position is not included with the peripheral equipment. An
appropriate bottle measuring 50 mm or less in diameter and 126 mm or less in
height must be provided.
When using the Single Sample Bottle, the lowest needle position is the upper
surface of the top plate of the Single Sample Position stand. The needle will not
reach the bottom of the bottle.
Bottle
Lowest needle
position
Lower stand plate
Screw
Lower screw slot
Figure 23. Single Sample Position
The mouth on the Single Sample Position bottle should be lower than the rinse
port. If the mouth is higher than the rinse port, remove the two screws holding
the lower plate and re-attach the stand it in a lower position.

2 Setup
Software Settings
Software Settings
The configuration procedure differs between G7201C (ICP-MS MassHunter 4.1

or later), G7201A/B, and G7200B ICP-MS software.
G7201C (ICP-MS MassHunter 4.1 or later)

Configuration
The term "ALS" has been changed to "Autosampler" in MassHunter 4.4.

NOTE
1 Launch MassHunter, and then click the Settings gadget on the dashboard.
The Settings dialog box appears.
2 On MassHunter 4.2 or later, click System, and then click at the side of
Hardware.
On MassHunter 4.1, skip this step.
3 Click Sample Introduction, and then select Use Autosampler in Properties.
MassHunter 4.1 MassHunter 4.2 or later

Figure 24.

2 Setup
G7201C (ICP-MS MassHunter 4.1 or later) Configuration
4 Click Edit in Properties.

The Configure Autosampler dialog box appears.
5 Click the Type tab, and then select Agilent I-AS from the Autosampler Type list
box.
Figure 25.
6 Click the Rack tab, and then select the rack that will be used from the
Autosampler Rack list box.
Figure 26.
You need MassHunter 4.4 or later to use the 28-vial sample tray (28 Samples
Rack). If the software installed is earlier than MassHunter 4.4, update to
MassHunter 4.4 or later.
The I-AS needle does not move to the correct position if the Autosampler Rack
CAUTION is not configured correctly.

2 Setup
G7201C (ICP-MS MassHunter 4.1 or later) Configuration
7 Click the COM Port tab, and then select the COM port number for the
Autosampler.
You should normally select the highest port number from the COM port
setting. (for example: if COM 1 to COM 3 are available, usually use COM 3)
Figure 27.
8 Click the Probe Depth tab, and then set the appropriate probe depth.
Figure 28.
Be very careful when setting the values for the Rinse Port, Tray/Single, and
CAUTION standard/Rinse to prevent the needle from coming into contact with anything
else. Start with smaller values and gradually increase until an appropriate
setting is achieved. If the value is too high, the tip of the needle makes contact
and may bend or break.

2 Setup
G7201A/B Configuration
9 Click the Other tab, and then select Escape Mode.
Figure 29.
10 Click OK.
11 Click Close to close the Settings dialog box.
1 Launch MassHunter, and then select Hardware from the task menu.
2 Click on the Mainframe illustration and select Communication... from the
popup menu.
Select Online from the Set Communication dialog box, check Use ALS and click
OK.
Figure 30. Set Communication dialog box

2 Setup
3 When the Autosampler (ALS) illustration appears, select the Autosampler from
Autosampler Type.
4 Select the number of samples from each available setting in the sample rack
list box.
Figure 31.
The I-AS needle does not move to the correct position if the Autosampler Rack
CAUTION is not configured correctly.
5 Click on the I-AS illustration and select Set Communication... from the popup
menu.
You should normally select the highest port number from the COM port
setting in the Set ALS Communication dialog box.

2 Setup
6 Click on the I-AS illustration and select Set Sampling Depth of I-AS... from the
popup menu.
Figure 32.
7 Configure each setting in the Set Sampling Depth of I-AS dialog box.
Figure 33. Set Sampling Depth of I-AS dialog box
CAUTION standard/Rinse to prevent the needle from coming into contact with anything
else. Start with smaller values and gradually increase until an appropriate
setting is achieved. If the value is too high, the tip of the needle makes contact
and may bend or break.

2 Setup
8 Configuring Escape Mode.

Click on the I-AS illustration and select Escape Mode from the popup menu.
Figure 34.

2 Setup
G7200B Configuration
Configure the I-AS to ensure proper operation.

The following procedure describes I-AS configuration.
1 Close down all tasks.
2 Double-click the Configuration icon located on the desktop.
The ICP-MS Configuration dialog box will be displayed.
3 Select Autosampler >> I-AS.
Select I-AS from the

Autosampler menu
Setup button
Figure 35.
4 Click the Setup button located to the right of the Autosampler menu.
The I-AS Configuration dialog box will be displayed.
5 Set the menu items in the I-AS Configuration dialog box.

2 Setup
CAUTION STD/Rinse to prevent the needle from making contact. Start with smaller
values and gradually increase until an appropriate setting is achieved. If the
value is too high, the tip of the needle will make contact and may bend or
break.
Figure 36. I-AS Configuration dialog box
6 After all menu items have been correctly input, click the OK button.
The I-AS Configuration dialog box will close, returning to the ICP-MS
7 Click the Save button in the ICP-MS Configuration dialog box.
The ICP-MS Configuration dialog box will close and save the new settings.

2 Setup
Settings
Settings
COM port for Set the connection port for the I-AS communications
Autosampler: network cable. If the software dialog box shows that
ISIS is using the port, this port cannot be used for the
I-AS.
Sample Tray: Select the sample tray type that will be used.
Single Sample
89 Samples: Type A, D, E, F
53 Samples: Type B
18 Samples: Type C
28 Samples: Type G (Available on MassHunter 4.4 or
later)
Sampling Depth: In the positions below, set the travel distance for the
I-AS arm from its highest to lowest positions. The drive
motor moves in increments of 0.125 mm. Any smaller
settings will be automatically rounded up to this value.
Rinse Port: Set the distance the arm will lower from its highest
point in the rinse port position. Start with small values
and slowly increase (approx. 10 mm) to avoid contact.
The maximum setting is 55.00 mm.
Tray/Single: Set the distance the arm will lower from its highest
point in the sample vial or Single Sample Position. Start
with small values and slowly increase (approx. 10 mm)
to avoid contact. The maximum setting is 63.75 mm.
STD/Rinse: Set the distance the arm will lower from its highest
point in the external bottle (for standard and cleaning
fluid) position. Start with small values and slowly
increase (approx. 10 mm) to avoid contact. The
maximum setting is 63.75 mm.
Enable ESC Mode: Check this to enter escape mode. In escape mode, to
move the needle over the sample tray, the sample tray
is moved to a pre-determined position before moving
the needle. The position of the needle in escape mode
will be the last (highest) sample number on the inner
radius of the sample tray, as illustrated in the following
table. Samples in this row cannot be measured in
escape mode.

2 Setup
Settings
Table 5 Location of the vial that cannot measured in escape mode
Tray Type Location of Vial Not Measured in Escape Mode
89-Vial Sample Trays (Types A, D, E) 32, 57, 77, 89
53-Vial Sample Tray (Type B) 25, 41, 53
18-Vial Sample Tray (Type C) 12,18
15-Vial Sample Tray (Type F) 57, 89 (At 89-Vial sample tray setting)
28-Vial Sample Tray (Type G) 22, 28
An executed analysis will stop if it is configured with a vial number unavailable

CAUTION for use in Escape Mode.

2 Setup
Checking and Adjusting Operations
Checking and Adjusting Operations
This section explains how to check the operation of the I-AS and install the
needle.
Checking Operations
Before operating the I-AS, check the following items.

CAUTION
• Verify that the settings in the software are correct. If the distance allotted
for arm travel is too great, the needle can contact the table, twist and/or
break off.
• Make sure there is nothing to interfere with the operations of the arm,
needle, and tubing. Any contact with foreign objects can damage or deform
the I-AS, and cause fluids to squirt or leak out of the instrument.
Using the G7201C ICP-MS Software

1 Turn the I-AS power switch on.
Verify that the LED on the front of the I-AS main body is on.
2 Initialize the I-AS before performing the operation check.
Click the Autosampler icon on the toolbar, and then select Initialize from the
pull-down menu.
Figure 37. Initialize menu

2 Setup
Checking Operations
3 Display the Select Vial dialog box.

Click the Autosampler gadget on the dashboard to display the Select Vial
dialog box.
Figure 38. Select Vial dialog box
Buttons
Home button: Moves the arm to the home position (directly over the rinse
port) and to the highest possible position without lowering
the arm.
1 button: Moves the arm to the first external bottle and lowers the
arm to the setting in the Select Vial dialog box. It then
closes this dialog box.
2 button: Moves the arm to the second external bottle and lowers
the arm to the setting in the Select Vial dialog box. It then
3 button: Moves the arm to the third external bottle and lowers the
Sampler Rack Displays the type of sample tray configured in the
Type: Autosampler Rack drop down menu.

2 Setup
Checking Operations
Vial No. [ ]: Click on the desired sample position number icon or enter
the sample position number in the edit box to move
sample position numbers.
The configurable sample numbers are displayed in the
Select Vial dialog box. External bottles are numbered 1-3.
Sample vials are numbered sequentially with four digits
starting from 1000 (1001, 1002,...). The Single Sample
Position is numbered 1000.
Go to button: Moves the arm to the sample position configured in Vial
No. and lowers the arm to the setting in the dialog box. It
then closes this dialog box.
Move button: Moves the arm to the sample position configured in Vial
No. and maintains the high position of the arm. The dialog
box remains open.
Close button: Closes the dialog box without performing any operations.
Pressing Enter key after inputting the Vial No.[ ] in the Edit Box will have the same
NOTE effect as clicking the Go to button.
4 From the Select Vial dialog box designate an optional vial, press the Move
button and verify that the I-AS moves properly.
5 Press the Go to button on the Select Vial dialog box and verify that the arm
lowers.
Each operation can be initiated from the pull-down menu of the Autosampler
NOTE icon on the toolbar.
Figure 39. Autosampler icon pull-down menu

2 Setup
Checking Operations
Using the G7201A/B ICP-MS Software

1 Turn the I-AS power switch to I to power ON.
Verify that the LED on the front of the I-AS main body is on.
2 Initialize the I-AS before performing the operation check.
Click on the ALS Initialize button on the toolbar.
Figure 40. ALS Initialize toolbar button

Click on the ASL Vial Number combo box to display the Select Vial dialog box.
Buttons
Home button: Moves the arm to the home position (directly over the rinse
the arm.

2 Setup
Checking Operations
the arm to the setting in the Select Vial dialog box. It then
Sampler Rack Displays the type of sample tray configured in the
Type: Autosampler Rack drop down menu.
Vial No. [ ]: Click on the desired sample position number icon or enter
the sample position number in the edit box to move
sample position numbers.
The configurable sample numbers are displayed in the
Select Vial dialog box. External bottles are numbered 1-3.
Sample vials are numbered sequentially with four digits
starting from 1000 (1001, 1002,...). The Single Sample
Position is numbered 1000.
Go to button: Moves the arm to the sample position configured in Vial
No. and lowers the arm to the setting in the dialog box. It
Move button: Moves the arm to the sample position configured in Vial
No. and maintains the high position of the arm. The dialog
box remains open.
Close button: Closes the dialog box without performing any operations.
NOTE effect as clicking the Go to button.
4 From the Select Vial dialog box designate an optional vial, press the Move
button and verify that the I-AS moves properly.
5 Press the Go to button on the Select Vial dialog box and verify that the arm
lowers.
Each operation can be initiated from the ALS icons on the toolbar.
NOTE

2 Setup
Checking Operations
Figure 42. ALS toolbar icons
Using the G7200B ICP-MS Software

1 Turn the I-AS power switch to “I” to power up the instrument.
Make sure the LED on the front of the I-AS illuminates.
2 Display the ALS GoTo dialog box.
There are three ways to display the ALS GoTo dialog box.
• From the ICP-MS top, select Acquire Data >> Main Panel... to display the
ICP-MS Acquisition window. From this window, select ALS >> GoTo to display
the ALS GoTo dialog box.
• From the ICP-MS top, select Instrument >> Instrument Control... to display the
ICP-MS Instrument Control window. From this window, select ALS >> GoTo to
display the ALS GoTo dialog box.
• From the ICP-MS top, select Instrument >> Tune... to display the ICP-MS
Tuning window. From this window, select ALS >> GoTo to display the ALS
GoTo dialog box.
Sample tray type
Figure 43. ALS GoTo dialog box
Buttons
HOME button: Moves the arm to the home position (directly over the rinse
the arm.

2 Setup
Checking Operations
arm to the setting in the I-AS Configuration dialog box. It
the arm to the setting in the I-AS Configuration dialog box.
It then closes this dialog box.
arm to the setting in the I-AS Configuration dialog box. It
Sample Tray Type: Displays the type of sample tray as selected in the I-AS
Vial No. [ ]: The number in brackets represents the sample number
that can be set. External bottles are displayed with the
numbers 1 to 3. Sample vials are displayed in four-digit
numbers, represented by 1000 and the sequential number
of the vial. (1001, 1002...). The Single Sample Position is
displayed as 1000.
GoTo button: Moves the arm to the location set for the Vial No. and
lowers it to the position in the I-AS Configuration dialog
box. This then closes the dialog box.
Move button: Moves the arm to the location set for the Vial No. and
keeps the arm at the upper position. The dialog box
remains open.
Cancel button: Closes the dialog box without performing any operations.
Help button: Displays the help screen.
NOTE effect as clicking the GoTo button.
3 Operate the buttons in the ALS GoTo dialog box and verify that the I-AS is
operating normally.

2 Setup
Installing and Adjusting the Needle
The procedure differs between G7201A/B/C and G7200B ICP-MS software.
Installation on G7201A/B/C
1 Set the sample tray or Single Sample Position to match the setting in the
software.
2 Click on the Autosampler illustration and select Set Sampling Depth of I-AS...
G7201A/B
G7201C
Figure 44. [Set Sampling Depth of I-AS...] dialog box

2 Setup
3 Input the following settings in the Set Sampling Depth of I-AS dialog box.
Figure 45. Set Sampling Depth of I-AS dialog box
Rinse Port: 55.00 mm

Tray/Single: 60.00 mm
Standard/Rinse: 3.00 mm
After inputting these values, click the OK button.

2 Setup
5 Click the Home button in the Select Vial dialog box.

Click the button with the needle unattached.
The arm will move to the home position.
6 Click on the position number that the sample vial is set to in the Select Vial
dialog box or enter the position number in the edit box.
The location of the sample vial on the sample tray is identified as a four digit
NOTE number consisting of 1000 and the number displayed on the sample tray (1001,
1002...).
7 Click the Go to button.

The arm will move to the sample vial location and lower.
8 Turn the knob located on the end of the arm counterclockwise to loosen.
Arm
Holder
Knob
Needle
Figure 47.
9 Insert the needle into the end of the arm.

Adjust the length to that the tip of the needles sits 2 to 3 mm above the
bottom of the vial. Tighten the knob on the end of the arm.

2 Setup
The needle is made of a pliable material and can bend easily. Handle the
CAUTION needle with care. Pay careful attention to the following when determining the
height of the needle tip:
• The tip of the needle should be completely submerged in the sample fluid.
• The tip of the needle should not touch the bottom of the sample vial.
• The needle should not touch anything when the arm is rotating.
If the needle is positioned too low, it can hit something when the arm is moving
and break, bend, or cause liquid to spurt or leak from the instrument.
Use the Micro Flow nebulizer needle when using a Micro Flow nebulizer.
NOTE
The lowest position of the needle should be the upper surface of the Single
Sample Position stand when using the Single Sample Position feature (See the
figure below).
If the tip of the needle is set lower than the lowest position, it will hit the tray
CAUTION cover and other objects when the arm is rotating.
Arm
Needle
The lowest
position of the
needle when using
the Single Sample
Position feature
Figure 48.

2 Setup
10 Display the Select Vial dialog box and click the Home button.
The arm will move to the home position. Verify that the needle is directly over
the rinse port.
Needle
(Home position)
Rinse port
Figure 49.
11 Display the Set Sampling Depth of I-AS dialog box.

12 Input 60.00 mm for the Standard/Rinse field in the Set Sampling Depth of I-AS
dialog box and click the OK button.
60.00 mm is the standard setting. Input an appropriate value so that the tip of
CAUTION the needle does not touch the bottom of the external bottle.
13 Display the Select Vial dialog box and click the 1 button.
The arm will move to the first external bottle location and the needle will
lower.
Verify that the needle properly lowers into the external bottle.
14 Connect the peristaltic pump tube.
• This step is not necessary if using a Micro Flow nebulizer as the Micro
Flow nebulizer needle is used. Continue to the next step.
• If a Micro Flow nebulizer is not used, attach the end of the tube coming
from the needle to the peristaltic pump tube.

2 Setup
Installation on G7200B
1 Set the sample tray or Single Sample Position to match the setting in the I-AS
2 Display the I-AS Configuration dialog box.
Figure 50. I-AS Configuration dialog box
3 Input the following settings in the I-AS Configuration dialog box.

Rinse Port: 55.00 mm
Tray/One Bottle: 60.00 mm
STD/Rinse: 3.00 mm
After inputting these values, click the OK button.

2 Setup
4 Display the ALS GoTo dialog box.

See “ALS GoTo Dialog Box” for an explanation of how to display the ALS GoTo
dialog box.
Figure 51. ALS GoTo dialog box
5 Click the HOME button in the ALS GoTo dialog box.

Click the button with the needle unattached.
The arm will move to the home position.
6 Input the location number of the sample vial in the Vial No. field in the ALS
GoTo dialog box.
The location of the sample vial on the sample tray is identified as a four digit
NOTE number consisting of 1000 and the number displayed on the sample tray (1001,
1002...).
7 Click the GoTo button.

The arm will move to the sample vial location and lower.

2 Setup
8 Turn the knob located on the end of the arm counterclockwise to loosen.
Arm
Holder
Knob
Needle
Figure 52.
9 Insert the needle into the end of the arm.

Adjust the length to that the tip of the needles sits 2 to 3 mm above the
bottom of the vial. Tighten the knob on the end of the arm.
The needle is made of a pliable material and can bend easily. Handle the
CAUTION needle with care. Pay careful attention to the following when determining the
height of the needle tip:
• The tip of the needle should be completely submerged in the sample fluid.
• The tip of the needle should not touch the bottom of the sample vial.
• The needle should not touch anything when the arm is rotating.
If the needle is positioned too low, it can hit something when the arm is moving
and break, bend, or cause liquid to spurt or leak from the instrument.
Use the Micro Flow nebulizer needle when using a Micro Flow nebulizer.
NOTE

2 Setup
The lowest position of the needle should be the upper surface of the Single
Sample Position stand when using the Single Sample Position feature (See the
figure below).
If the tip of the needle is set lower than the lowest position, it will hit the tray
CAUTION cover and other objects when the arm is rotating.
Arm
Needle
The lowest
position of the
needle when using
the Single Sample
Position feature
Figure 53.

2 Setup
10 Display the ALS GoTo dialog box and click the HOME button.
The arm will move to the home position. Verify that the needle is directly over
the rinse port.
Needle
(Home position)
Rinse port
Figure 54.
11 Display the I-AS Configuration dialog box.

12 Input 60.00 mm for the STD/Rinse field in the I-AS Configuration dialog box
and click the OK button.
60.00 mm is the standard setting. Input an appropriate value so that the tip of
CAUTION the needle does not touch the bottom of the external bottle.
13 Display the ALS GoTo dialog box and click the 1 button.
The arm will move to the first external bottle location and the needle will
lower.
Verify that the needle properly lowers into the external bottle.
14 Connect the peristaltic pump tube.
• This step is not necessary if using a Micro Flow nebulizer as the Micro
Flow nebulizer needle is used. Continue to the next step.
• If a Micro Flow nebulizer is not used, attach the end of the tube coming
from the needle to the peristaltic pump tube.

3 Operation
Measuring with Single Sample Position (Using the G7200B ICP-MS
Software) 64
Measuring with Single Sample Position 64
Single Sample Position Run Dialog Box 65

3 Operation
Measuring with Single Sample Position (Using the G7200B ICP-MS Software)

(Using the G7200B ICP-MS Software)
Verify the following before beginning measurement with the Single Sample
Position feature:
• Is the sample tray type field set to “Single Sample” in the I-AS Configuration
dialog box?
Figure 55.
• Has the sample tray been removed and the Single Sample Position properly
set up?
• Is the sample vial number set at “1000”? (The vial number for Single Sample
Position is “1000”)
• Is the LED on the I-AS illuminated? (If not, make sure the power switch is ON.)
In addition to setting the sample vial location to “1000” when using the Single
Sample Position feature for measurement, make sure the other settings are the
same as when using the sample tray.

3 Operation
Single Sample Position Run Dialog Box
The Single Sample Position Run dialog box can be used to run single samples.
Use the following procedure to display the Single Sample Position Run dialog box.
1 From the ICP-MS Top window, select Acquire Data >> Main Panel.
The ICP-MS Acquisition window will be displayed on the screen.
2 In the ICP-MS Acquisition window, select Acquire Data >> Single Sample
Position Run.
The Single Sample Position Run dialog box will be displayed on the screen.
Figure 56.

3 Operation
The following procedure explains how to use the Run Options in the Single
Sample Position Run dialog box.
PreRun Uptake/ Select this option to move the needle to the single sample
Stabilization: position before measuring (collecting data). If the needle is
already in the single sample position, it will not move.
When the PreRun Uptake/Stabilization check box is selected,
the system will follow the settings in the Peristaltic Pump
Program dialog box and uptake (replace) the sample
(increase the peristaltic pump speed) and then stabilize
(return the pump to its original speed and wait a specified
amount of time) prior to measuring.
Unselect the PreRun Uptake/Stabilization check box and the
system will bypass the sample uptake and stabilization
processes, and proceed directly to measuring the sample
(collecting data).
PostRun Rinse: Select this option to rinse the needle (includes rinsing at the
rinse port, and cleaning external bottles and sample vials)
according to the Peristaltic Pump Program dialog box
settings after the measurement (data collection) is
complete.
Unselect this check box to bypass the rinse process. The
needle will remain in the bottle after measurement is
complete.
Data Analysis: Select this option for analysis by method.

4 Maintenance
Daily Maintenance 68
Power Cable Fuse 69

4 Maintenance
Daily Maintenance
Daily Maintenance
Perform the following to maintain normal operation of the I-AS:

• Peristaltic Pump Tubing.
Change the Peristaltic Pump Tubing in the following cases:
When the tube becomes too loose to uptake a solution.
When the inside of tube is damaged and dregs come off.
When tubing in the above conditions is used, rinse fluid could overflow from
the rinse port, or dregs tend to come off and result in an impure rinse fluid.
• Use only filtered purified rinse fluid to prevent clogging.
If the Autosampler is not going to be used for a long period of time, replace
the rinse solution with pure water. This prevents the rinse port from clogging.
• Do not use rinse fluid that will harden when mixed with the samples.
• Always keep the rinse system clean.
• Clean the housing with a cloth slightly moistened with water. Do not allow
water to get into the inside of the device.

4 Maintenance
Power Cable Fuse
Power Cable Fuse
To replace the fuse for the power cable, open the fuse holder and replace.
Use a 2AT (time delay) fuse.
Confirm that the power cable has been removed from the wall outlet before
CAUTION replacing a fuse.
Fuse holder
Power switch
Power cable
connector
Figure 57. Fuse holder

This page is intentionally left blank.

In This Book
The Integrated Autosampler (I-AS) explains
the procedures necessary to use the
Integrated Autosampler to enhance sample
analysis.
www.agilent.com
© Agilent Technologies, Inc. 2018
Printed in USA
October 2018
*G3160-90027*
G3160-90027 Revision A

Integrated Autosampler (I-AS) : Agilent ICP-MS

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Agilent ICP-MS

Integrated Autosampler (I-AS)

1 Integrated Autosampler Overview and Configuration 5

Integrated Autosampler (I-AS) 3

4 Integrated Autosampler (I-AS)

Symbols Used on this Instrument 18

The Integrated Autosampler (I-AS) is a very flexible automated sample

Integrated Autosampler (I-AS) 5

Figure 1. Agilent 7800/7900/8900 ICP-MS equipped with the I-AS

6 Integrated Autosampler (I-AS)

Figure 2. Agilent 8800/7700 ICP-MS equipped with the I-AS

Integrated Autosampler (I-AS) 7

Sample tray Main unit

I-AS operation is controlled by MassHunter Workstation.

8 Integrated Autosampler (I-AS)

Integrated Autosampler (I-AS) 9

Main Unit Components

Power display LED

Figure 4. I-AS Components

10 Integrated Autosampler (I-AS)

Power Display LED

Integrated Autosampler (I-AS) 11

Sample Tray Max. No. of Vials Sample Vial Specifications

Type D 89 1.5 mL or 2 mL, 18 mL

Type F 15 Used for 48 (OD) × 55 (H) mm vial

Figure 5. Sample Tray Types (Photo Shows Type A)

12 Integrated Autosampler (I-AS)

Single Sample Position

Bottle for Single

Figure 6. Introducing a sample with Single Sample Position

Communications network connector

Integrated Autosampler (I-AS) 13

Power Cable Connector

Communications Network Connector

Rinse Fluid Inlet

Rinse Fluid Inlet Tube Connector

Rinse Fluid Outlet Tube Connector

14 Integrated Autosampler (I-AS)

The following specifications are subject to change without notice.

Dimensions: 56 (Diameter) x 61 (H) mm

Single Sample Position

Integrated Autosampler (I-AS) 15

The following utilities are required to operate the I-AS:

Secondary fuses in DC 5 V and DC 24 V line

16 Integrated Autosampler (I-AS)

This device complies with the requirements of EN550011, Group 1, Class B.

Use the I-AS only under the following operating conditions:

Integrated Autosampler (I-AS) 17

Symbols Used on this Instrument

The following symbols are used on this instrument:

Protective grounding terminal.

The Integrated Autosampler is identified with a serial number on a label on the

18 Integrated Autosampler (I-AS)

• Do not use organic solvents and other combustible or flammables in the

If an overflow of the rinse receptacle caused by blockage of the peristaltic

Integrated Autosampler (I-AS) 19

Precautions when moving the I-AS are listed below:

Verify the following before beginning measurements with the I-AS:

• Do not use organic solvents and other combustible or flammables in the

20 Integrated Autosampler (I-AS)

Setting up the I-AS 24

Checking and Adjusting Operations 46

Integrated Autosampler (I-AS) 21