Scribd Logo
Upload

Welcome to Scribd!

  • 14 views

    RTM Practicals

    Uploaded by

    Unza Waqar

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content

    You might also like

    RTM Practicals

    Uploaded by

    Unza Waqar
    14 views32 pages

    Original Title

    RTM practicals

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Report this Document

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    Download as pdf
    14 views32 pages

    RTM Practicals

    Uploaded by

    Unza Waqar

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
    Flag for inappropriate content
    Download as pdf
    of 32
    Date o : | PERSONAL PROTECTION EQUIPMENTSJ-—— | tion. equcpments protect eg Stim Personal _psatec blood. aisborne pathogens | Aor 4. | Fos paatection | Weas, aprons O% 3. | Fok protection a 4 | Face masks oh _| protection c 5. Always wear psatectiion —______— 6-| Fos hearing protection, Wwe must use earplugs. _ as Always wash got ty _evsuse Aygiene : and eth substances of eth» _qaggitd Ok lasses. shauld be used. fab taats 39%. body papvection, P -honds, wearing gloves Is nuit i sespitadors are wed 0X Heat in laboratory aoe foot enclosed shoes we hands with soap ov hand-was ‘steel Neve vaccum unloys ey shielded. | tw —deaige “f Shi ie _| Never handle token glans ul with base hands Use | oy deat par Aluays diy bins —<$ 5 Use a bAeirp te cean-up broken glas , broken alas m2 gla bin on shay ard nd in & genexal waate bin - tmmerse__hot. glassware in_cold wal , tk nau not ei aware Prcid__ exposure Press one ae a should _be chetkea. glaws ree aia, any, : i meaue is used ARES ok DNA as well gs rie is uet ent th Pre, Sos eed Lapa ae [componenr ne se 4 _yuseal 40 phat ah ent dated Tie Various componerita Aegutred Rt wh DNA_psirners z ae PCR include a DNA [sample be DNA Meena SO ee polymesa ‘ Bs fergie, : | NSTRUMENTS USED:- R_ machine _(Thamocycler) PCR tubes F Date ¢ | the temperature is then _Covtes down betueen yo and |The. precise temperature 1» cuttial ond PCR systers has t be. defined and opli mised » | Decrea eta in 7 ature _allpw5 bending of piimer ea6 of / ir gle_atrand of DNA we an 4a | a ee “ool the _hyb sai gui psiemers. The a ed ee act as primus 4a DNA polymeare The___third _Atep_ extension _in_ which DNA ts camued oul by 2 thesamnostable DNA polymsrtire . commoly talad Tay, DNA pos merase the temperature iu _saiszed upto ZC PCR_eye. fasta 10-™/nules ancl the amount of DNA HIGH PERFORMANCE LIQUID CHROMATOGRAPHY. PRINCIPLE :- ares eee | The basic _psimciple of High Pargormance _Liquiel _|_chiomate raphy (HPLC) is “that the comple p_gorced. E | __ fraugh acolunn of the stationary _phare pail a) | pumping a Oui at high _prcwute through the Sar vil |_ column. ee. ee ee | ipa) rece |e ee COMPONENTS OF HPLC:- eeaataias 2 ‘5 Colunn:- St is _madeup _of stainless steel which tan ss uithstand piessuse pte 50 MP. the length of _ column —__| varies between 5-25 em with Internat _ditameler > 4-Smn§ The gi Pop mobile phase _fprough the cob i 4- 3m min | Stationas ae ei = = | salient prose in prod up of an adsosbent |) _ mated tat hay voy smal paslete size Te _particle || sige Kept uniform — b_oblain __béler_pesformance._ ally chemically rmodijretd Aileen plans — aie as tationa pha vet Me g a |_ [sua are Sample Injector :- i Launple Injector allows inthioducter, } Sf sample ints the column . Se _ls__ present 4st depp the column Detector :- : Sr_oider ty detect the eluale (sample coming cut of folumn » the HPLC colemp 4 attached wit detector Cortat of —deteclou i.e W detector, TR deteziny we index. detector , Mars spectiomnelin, Electrochemical detector, ele can be wed Chast econder ter :— eu i dy cletectoxr and collect; inppundlion about ekution LINORKING OF HPLC: . HPLC, alo Known ap High Presuse Lil elrsom ato Pf ox high presse uid ehtematopaphy is a modiplect colums ecanaiane ie ie cn P JAN ‘NTMI | Glucose i / Sucrose | (sin) Time (8 min 0 unlsnouin ia peak obtained tn, cheat am minutes indicate Kno peak at glib om “mare mkt are T_peak obfained at | Paesernce fatcre in bles | xe ino sample ote n— Joe _dample detection , we must have standard iol TO _ FIND CONCENTRATION OF €aMpOUND IN conten ation compound Zt have standasd cue. ss in IS ert vation , IM area wrdler tho peek in unknown _ —

    You might also like