TUASON, MA. LORAH YZABELA L.

MEDTECH 2-Y2-2

LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria

Guide Questions.
1. What kinds of organisms are destroyed when your desktop is scrubbed down with a
disinfectant?
Vegetative cells & viruses

2. Are bacterial endospores destroyed? 

 No, bacterial endospores are not destroyed.

3. How hot should inoculating loops and needles be heated? 

 Until red hot

4. Why is it necessary to flame the mouth of the tube before and after performing an inoculation?

 To prevent contamination of microorganisms. Flaming the mouth of a test tube creates an
air flow. Hot air rises. Therefore dust/particles in the air are less likely to fall into your tube.

5. Why is it important not to dig into the agar with the loop?
 The bacteria can grow in the gouge preventing the bacteria from growing into distinct
colonies. It is important to avoid digging into agar with the loop due to the high risk of
cross contamination between different specimens. Contamination renders a petri dish or
streak plate unusable. The American Society for Microbiology states that improper loop
sterilization also increases the risk of contamination.

6. Why must the loop be flamed before entering a culture? Why must it be flamed after making
an inoculation?
The loop is flamed before entering a culture tube to ensure that no contaminating
microbes are introduced in to the culture. The loop is flamed afterward so that no culture
microorganisms are introduced into the working environment.
 REFERENCES

IAC Publishing. (n.d.). Why is it important to avoid digging into the agar with the loop?
Reference. Retrieved February 21, 2022, from
https://www.reference.com/science/important-avoid-digging-agar-loop-e9460ed516494f58

Microbiology lab questions flashcards | Quizlet. (n.d.). Retrieved February 21, 2022, from
https://quizlet.com/171064527/microbiology-lab-questions-flash-cards/

Microbiology midterm flashcards | Quizlet. (n.d.). Retrieved February 21, 2022, from
https://quizlet.com/73552210/microbiology-midterm-flash-cards/

Pure Culture Techniques I. Objectives. (n.d.). Retrieved February 21, 2022, from
https://www.sas.upenn.edu/LabManuals/biol275/Table_of_Contents_files/5-
PureCulture.pdf

Why should you flame the mouth of a test tube? (n.d.). Retrieved February 21, 2022, from
https://www.quora.com/Why-should-you-flame-the-mouth-of-a-test-tube