EX NO:S|| EsTIMATION OE DNA

24-o1-20
ALM
To L>timele Ie amaunt 5 DAA oreent

im a whole l ANen bolutio

RINCPLE
DNA io skimated usimg diphenaLmunc
e
NeageCat The deoxymbose îm llte DANA
eene_ a i d somms hydmoxy Revune aldehpede
which acts LwitR diphent amia qive, aa blue
ColouxCeoplex he intensity0y e CRus derelp b
aead a t boomm Cing re Hllex

KEA GEoS REQURED:

C SBocl shandaxd stuthon DNA

DNA 6 dissplved im LUdml (
Lopma
Akdled waker .

LCmeohxationloALqme
COnd
Umal me
DN
di2 hlonking ehandand Sriution andard DNA Selution
5ml Stoc
diluted 50ml handard stas Using
deulled wate

Ceomenhation -LODuglmlS eelmglmc)

Contentts in he
Les tube Blank lanking standaxd sSdutionnnoon Solulion
TO Ta
Wosuing tandardd
Sduion Cm DS1-o 2-0 |2.5|
5 oro 200 2Le
Conantvaion Ak
OnLnown boluionCm oS

Dgiled water Crn) 2.5 2.o s 0 S | 2 s 2.5 2.0 2.0

Ppheu amme
Reanent
The Contents a mxed wetl and tepin a
boi uake alt o lominuukps. and
allodued to looL

Cphical densit
b.o 600.0b |00q -12 O45|0-03 D.03 |o.bb oDb
ua ed at boonm
 2 Diphen amine Reagent:
Above lgm dipheny amine
dicsolved in lcom ug Raial auehc cuid and 2.sml
Cmeahated Sulphumc aud ó added.

fkocEDURE
Exacku
pipelted ouk 0.2m Im
woncing standaxd olution_imio a Senes cleanday
tes tuhe maiad cs SLS2,S2S4& S
mespectivelj
The given uunlcnnwn Solutiom & made upls sme
wimg- dislilled watex 0.2m, aod o lml G
wolcnouor oluion ia kco íow atwo dap alean
test tuhe abelled as TeTa respeckivels.and l Wlome
made up lo m uaimg dúlilled wate. mR (
distilled waUtax alome Semves as blaalc hoa 5mdO
diphtn mine_reageas added s al dke test tube
and mixedit- we Heat dhe Cumtente im la
oiing
boiling water baalz_Aor lOminute> Ater orotng
lte ophical_deasihs G mewused a oupmm usong
ed silke

The amount GDNA reent in k ive

unlenouwr Sduhon Csbme) 2qual s.
 C
T

C n btentnown Contcis oY* DavN

Onlanad Cen tats -

So m Untncwn Contaiis

05 unlenouWN Conrain oy DNA

)m nlenowo Cnnrain

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