Methods in Enzymology

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Part D: Enzyme Structure

C. H. W. Hirs, Serge N. Timasheff

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Volume 27,
Pages 3-1063 (1973)

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Contributors to volume XXVII, part D
Pages ix-x

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Editorial No access
Preface
C.H.W Hirs, Serge N Timasheff
Page xi

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Volumes in series
Pages xiii-xv

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[1] Ultracentrifugal studies with absorption optics and a split-beam photoelectric
scanner
H.K Schachman, Stuart J Edelstein
Pages 3-59

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[2] Determination of molecular weights in the ultracentrifuge using time-lapse
photography
J.L Bethune
Pages 59-67

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[3] Active enzyme centrifugation
David L Kemper, Johannes Everse
Pages 67-82

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[4] Measurement of partial specific volume by sedimentation equilibrium in
H2O D2O solutions
Stuart J. Edelstein, H.K. Schachman
Pages 82-98

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[5] The determination of the partial specific volume of proteins by the mechanical
oscillator technique
O Kratky, H Leopold, H Stabinger
Pages 98-110

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[6] Density gradient sedimentation equilibrium
John E Hearst, Carl W Schmid
Pages 111-127

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[7] Proteins in density gradients at sedimentation equilibrium
James B Ifft
Pages 128-140

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Discussion Abstract only

[9] Zonal centrifugation
David Freifelder
Pages 140-150

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[9] Small-angle X-ray scattering
Helmut Pessen, Thomas F. Kumosinski, Serge N. Timasheff
Pages 151-209

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[10] Light scattering and differential refractometry
Eugene P Pittz, James C Lee, Barkey Bablouzian, Robert Townend, Serge N Timasheff
Pages 209-256

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[10a] Osmotic pressure
Guido Guidotti
Pages 256-269

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[11] Sedimentation velocity measurement of protein association
Lilo M Gilbert, G.A Gilbert
Pages 273-296

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[12] Measurements of protein interactions mediated by small molecules using
sedimentation velocity
John R Cann, Walter B Goad
Pages 296-306
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[13] Pressure effects in ultracentrifugation of interacting systems
William F Harrington, Gerson Kegeles
Pages 306-345

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[14] Characterization of proteins by sedimentation equilibrium in the analytical
ultracentrifuge
David C Teller
Pages 346-441

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[15] Studies of protein ligand binding by gel permeation techniques
Gary K Ackers
Pages 441-455

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[16] Determination of equilibrium constants by countercurrent distribution
Gerson Kegeles
Pages 456-464

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[17] Rapid measurement of binding of ligands by rate of dialysis
F.C Womack, S.P Colowick
Pages 464-471

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Research article Abstract only
[18] Chromatography of proteins on hydroxyapatite
Giorgio Bernardi
Pages 471-479

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[19] Rotating cylinder viscometers
Elliott L Uhlenhopp, Bruno H Zimm
Pages 483-491

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[20] Solubility measurements
Yasuhiko Nozaki
Pages 491-497

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[21] Ultraviolet difference spectroscopy—new techniques and applications
John W Donovan
Pages 497-525

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[22] Spectrophotometric titration of the functional groups of proteins
John W. Donovan
Pages 525-548

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[23] Difference infrared spectrophotometric titration of protein carboxyls
Serge N Timasheff, H Susi, John A Rupley
Pages 548-557

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[24] Differential conductimetry
Alkis J Sophianopoulos
Pages 557-590

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[25] Thermal titrimetry
Mario A Marini, Charles J Martin
Pages 591-616

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[26] Negative stain electron microscopy of protein macromolecules
Robert M Oliver
Pages 617-672

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[27] Circular dichroism and optical rotatory dispersion of proteins and
polypeptides
Alice J Adler, Norma J Greenfield, Gerald D Fasman
Pages 675-735

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[28] Difference optical rotatory dispersion and circular dichroism
Jen Tsi Yang, Kue Hung Chau
Pages 736-750

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[29] Analysis of optical activity spectra of turbid biological suspensions
Allan S Schneider
Pages 751-767

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[30] UV absorption and circular dichroism measurements on light scattering
biological specimens; fluorescent cell and related large-angle light detection
techniques
Burton P Dorman, John E Hearst, Marcos F Maestre
Pages 767-796

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[31] Magneto optical rotation spectroscopy
Victor E Shashoua
Pages 796-810

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[32] Differential spectrofluorometry
Gerald D Fasman, Barkev Bablouzian
Pages 811-822

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[33] Nuclear magnetic resonance spectroscopy of proteins
W.D Phillips
Pages 825-836

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[34] The prospects for carbon-13 nuclear magnetic resonance studies in
enzymology
Frank R.N Gurd, Philip Keim
Pages 836-911

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[35] Mössbauer spectroscopy
Thomas H Moss
Pages 912-941

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[36] Automated edman degradation: The protein sequenator
Hugh D Niall
Pages 942-1010

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Author index
Pages 1011-1035

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Subject index
Pages 1036-1063

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ISBN: 978-0-12-181890-6
ISSN: 0076-6879

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