3/16/22, 5:04 PM Study of local anesthetics by Surface anaesthesia on the cornea of rabbits - Labmonk

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Study of local anesthetics by Surface anaesthes

cornea of rabbits
Pharmacology

BACKGROUND
Local anaesthesia is a technique to create loss of sensation in specific region of the body.1 Usually they are us
procedures, cesarean section with reduced pain and distress. This technique is safer and superior to general ana
Local anaesthetic drugs produce reversible effect and a loss of nociception. When these drugs are used on s
show effects such as analgesia and paralysis. Local anaesthetics differ in their pharmacological properti
techniques such as surface (topical) anaesthesia, infiltration, epidural block, plexus block and spinal anaesthesi
The aim of the present study is to determine the activity and tolerability of new local anaesthetics after surface
rabbits.

REQUIREMENTS
Animals required:

Albino rabbits of either sex weighing 2.5-3 Kg

Drugs:

Lignocaine 0.2%, test drug (0.05% and 0.1%)

Instruments used:

1 ml syringe (22 guage needle)

PROCEDURE
Place the Albino rats in rabbit holding cages. Trim Both the upper and lower eyelashes carefully. Held open th
to form a pouch. Instill 0.5 ml of test solution for 30 seconds. Then repeat the same procedure, so that fina
within 1 minute. Similarly instill 1 ml of standard drug (lignocaine) within 1 minute to the other eye. Test the
eyes for every 5 minutes with wet cotton plug. Start the test within 5 minutes after application of the drug
minutes till anaesthesia disappears and corneal reflex reappears again.

OBSERVATION

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3/16/22, 5:04 PM Study of local anesthetics by Surface anaesthesia on the cornea of rabbits - Labmonk

Drug Onset of surface anaesthesia

Duration of surface anaesthesia
(in min)
(in min)

Right eye Left eye Right eye Left eye

Lignocaine (0.2%)

Test drug (0.05%)

Test drug (0.1%)

CONCLUSION
Effective local anaesthetic ceases the corneal reflex produced by one touch of the cornea. Onset and duration o
effect of local anaesthetic.

REFERENCES
1. William A, Catterall A, Kenneth M. Local Anesthetics. In: Laurence Bruton L,Goodman and Gilmans T
Therapeutics, 12 th ed. New Delhi: Mc Graw Hill Medical; 2011: 564.

2. Local anesthesia. Available at: https://en.wikipedia.org/wiki/Local_anesthesia. Accessed on 17 October

3. Vogel GH. Surface anaesthesia on the cornea of rabbits. Drug Discovery and evaluation-Pharmaco
Newyork: Springer-Verlag; 2002: 655-656.

Editorial Team
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