University of AlKafeel

" Technical Laboratory Techniques "

Stage 1 / group A2
By: Rawan Odai Abbas

Subject / Lab-laboratory instrumentation

Report title / ( centrifuge )

To : Ahmed Makki Naji Maalh
Centrifuge
Centrifugation is a
laboratory device used to
separate liquids, based on
density, separation is
achieved by rotating in a
container containing high-
speed materials, the
centrifugal force pushing
heavy materials out. This
device is found in most
laboratories from the blood
laboratory to the clinical
examination laboratory to
Research: There are many
types of centrifuges, which
can be classified
according to use, there are
many classes available for
.the researcher

:The working principle of centrifuges is based on

.Rotational movement 2- The centrifugal force -1
Types of centrifuges :

1) Benchtop Centrifuges
It is a wide class of centrifuges, the maximum speed can
range from a few hundred to 50,000 g, the tube volume
can range from less than 1ml (such as PCR tubes) to a
few liters, different types of rotors such as the fixed angle,
.and the continuous flow are Also usually interchangeable

2) Centrifugal chiller
They are ideal compact tools for centrifuging samples
that may be temperature sensitive, they feature many
interchangeable rotors and adapters to accommodate a
wide range of sample storage units from less than 1 ml to
a few liters, speeds can also vary, and some speeds can
reach 60 , 000 x g

3) Clinical centrifuges
They are compact low-speed centrifuges ideal for
separating entire blood components, such as serum,
plasma and red blood cells, as well as other bodily fluids,
their speeds can range from 200 rpm to 6000 rpm, most
clinical centrifuges can accommodate intubation tubes
Common blood, but be sure to review each supplier for
specific tube sizes, check out a variety of clinical
centrifuges from different manufacturers
4) lntensive centrifuges
Intensive centrifuges can use the centrifugal
force,temperature to remove liquid solvents to concentrate
or dispose of samples, this tool is ideal for purifying or
preparing samples such as nucleic acids, proteins,
peptides and other compounds for a variety of research
applications, for solvent evaporation the centrifuges are
used Vacuum is usually integrated heating systems

5) Precision centrvesines:
are the basic tools in many research laboratories that
overall ultimate lab sizes such as 2 ml, 1.5 ml, 0.5 ml and
PCR pipes, small centrifuges of routine laboratory actions
are usually accessed at speeds up to 16,000 g, while the
specialized tools can reach up to 30,000 g, In addition,
manufacturers may also provide manufacturers, switching,
compared to a variety of different microcentrifugs the
.temperature surrounding and motorlessrifuges chilled