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Chemistry Lab 1
Chemistry Lab 1
Chemistry Lab 1
1ToPiepaje
Experiment
m:
altBo Using this soluteto.
ddexmine.. the
molaitystrength
o ve0.unknouon Mn04 Solutíon.
F.A:S stand ard Sol?6-92
0ptepane M|20
Htnd.out. this.le. punity.ompuxe KMht;
Sosample.l.gm..as.whlth.have.bean.
dissolvepe iihe
.
molaity.strehgth.o qiven.un
khoon R MnOu.So
6To.separale AHeLolour Gomponant In th 39-24|.
ven louDe leaves...by.the 4Scendirg
papes.chromotography determme.
their R£ Yalu
Tosepamate Cu-RcdT.1on.preseit.in the20-9-21.
oahg.qscendir bapa.
a(ven.mxtune
chromat0gaphy.detexmine. thejt.
RfNalud
8: Jo study some simple..teat
.
****
5 n.the qiven Morgan salt.to LOntihm821
he.rethe ane..catan4.ane
anton
16 T6 deect -the.-funttional..Raup41
phcbekt... teh....0iGani .-
Compothd:.
11 deteet theHnctidadl Joep.phcoj1b.1-24
A. gen
CSganic compoMnd.
480detecd-the.anttbionalRonp 3 11r
HAelert..u. ieh.ahi Compouhd.
19-1-eletect tht-huncbiaral.ieup.. 11221
pesert..niven..ganit..
--
compohd,
020Tddteal.thetunebsnhali.sRoup. 22
h.xen Agahi..cOmpouhd..
0 detecttharchonal. 22
0pA. ...
LAmYauh...
en.. dgaNt.
.. .-
.I.
Page No. .. **** Date23.8.-2|.
HExPERIMENT 1 #
im-
To pepaie 25om DM20o Sotutfon oMoh 13
sat o emos Ammonium sulphate.
Fesoy CNH4)S04 6H2OP
* HppayaTus
Hatch alass, oeht bo box
mactfonal oefght box
25om& beaKe iass HOd 225o m measuring.
Rask ash bottle
Chemica Reautned
Mohst'2 Lqlt conc HaS04 and dfstt0ed Oate
M = MasS X IooO
2-0 392 X 2S0
Mass 392X250
20 XIbb
4 9gm
Paiocedue
Teacher's Signature
Page No. Date... **
2 Weghthe
on
accunately
occtch
43 gm o moh 2at stal
q&asSs
3 1anste
Mom
the caneuly the oeighed mohy Salt
the oath aass unto clean 25oml meqsurhg
ask Add one test tube dil HaSoy then add
the distilled wate up to the. mank o s ask
Obsevatton
Result:
M Mohts Salf solutton ús abel úB eauiicd
Solutton.
Pote cautfons:-
ODo not oe
p
to add 5m conc. Ha$oy beoe
beone
makin
Li)heahíng the moh' 84Jt Should be accurate
Teacher's Signature .
Page NO.. Date.p 3
+ExPERIMENT-2
Appaiatus
atch atass oeiaht box dactiong uoeght
bo 25om beake lasS siod 250 md measuring
ask and uoash bottle
MaTetra Requbted
Oxalic acid and distilled waten
Theo
Molecul qmass ot oxaltc acd
CooH
CooH
ana
2H20 (coot) 2H20 126gm
Molatit mass|m olaculaa mass
Votyme Soluton in lityeg
Mass YI0O0
20
Obse vation I-
Resyl
M20 Oxalic acid solution fs Jeveis eautned
Soluttom
Precautions
Heighing the Oxalic acid accu HatEly
(AaloD
Pay
EEXPERIMENT-3 H
To pxepa>ie standq>id solutton
ammonum Sulphate CF-A:S)
M20 deto ug
o
o
moh's 3alt 25o m
usin this solutonto deteimfne, the
sng mola>ft
sTnength oaiven unkno uon KMnOy Solutton.
Theo1i
The ieactton betuoeen moht Sat and kmnDy
un actdic medlum is edox eactton A2 mob2
Ths eactton can be 0titten as
Molecula Reacttons
2kmnOy t312SOy K2s0y +3H20 +2mnsOy 5CoJ----
PMeduch on half
2feSou CNH)2s0y 6H20 tH2s0y t Coj fe.2 SOy)3t 13H20 t
2CNHy)2S04 x5
Dxudation half
2kmnDu t8H2SOy+ Iofes0y.(NHyJ280y 6HR0 KSoyt2mnsoy
5fe2soyl3 t
loNHyaSOy ft b8H2 0
DYexa Q0 edoycactton
Requttement
fppaatus
Buctte 20m P1PeFte ten ask bunette|
stand Oatch qass uoeight bod Chemal balqnce
02 yogp
2x M
0Hd02O 2 o
20M
260 Oo -001M
1StuengthokMn04 Solution
(gm/utue
vobsirSOVd= molqH+ Y Y MoleCula mass
LXI5&
loo 5 m Jt>He
Remarks ************"
250 meas utng
ask
Chemicqs
joys Ammonium sulphate, 1ystalline kmnOy
Solution cont. Hasoy
Tndliate's
KMn Oy us a sel
tndiator
End point
Colouless to at pot pink (kmn Ou aken n.
bunette
Obsevaton
Werant oatch atass =2m
Weight ooatch alass t moh %qlt 9- m
delaht mon salt =4.3m
olume moh's 3att Soluton pxepaned 25om0
molaaity moh's salt soluton
M[290tratfon
Volyme moh's Salt takenor each AtHetten =YOm
O0 m
20
1.8m
O m 2-0-
1-8m
O m
Conco1dant volume 20 Oml
Resutt The mdasity unknouorn kmmOy s O0m 6
stength ús 58 gm |ittHe
Teacher's Signature.
( ukQu
Page
N0. . EEXPERImENT-4-H
Date. * 2*****
Aim
To pePne m20 oF-A.s stand id Solutton
find
sotutfon
out the
percentage putity. ampue. kmnoy
(Sampl 2.0gm as which have been dšsolve
t
peH itie.
heosy i
The aeaon Mo hssalt and kmnoy
beruoeen
An acidt medtum
isiedox xeaction As'mohais salt
n aidi mediumn iS a 200d eduding. aqent Tho
sieatton can bee uOitten as
Moeculax Readon
MmOy t3H2S0yKs0u t2MnSoy s(0)t3H26
educhon hul
|L24eSOy (NHy)2 Sou 6H20-t HaSOy t{0)te2lSOu)3t 13Hh0
+2NH)2 SOy 3x5
ozidaton hal
20mnOy t 8Hesoy t 10feSou CNHYLSOy btieDKso yt aMnsoy
t68 H20otS fral8Ou)31
t16CNHy)2SOy
COxeral redoX Yegchon)
Ion equation
MmOy+8HT+ 5e Mnt-tHaO-. reduchion hatf
KMnOy
kMnOt
eact with
Moh%
o mole o
Balt tmohs eleqathat
sqtt
2nmo b
MKMnOy Vsmn Oy
MMohas satt x VNoha's gqlt
To
Hete
MKMnoy= MolgXity o kmn04 Solution
VkMOý volume o kmn04 Solutiom
h VMohr's saH VolymeMoh1% salt
ddietE
Mmohr's
MkmnOv
sqt =Molqnity o
2x
lo
2xM26 XO
Mmoht '
Moha sqlt
lo o
0 01M
femoug Ammonfumm
texxoup sulphate. ygtalzs kon0y.
Solutfon Con HSOy
heony
ne tcaztion befo een oxalic atd and kmnOyin
acidic medfum is a edox as acd
un
KMnOy
actdic
un
medium sa20odús a
acdic medi um
zieadtron
teducng
20od
oxalfc
agent and
oxidi'stnq agen
The eaction can be OHitteen as
Moleulasy Reation uloy
2KMnOyt3HsOy Ksot2Mn SOy t3Ha0 t5[o3 -.
sieductionhatf
CapH+
COoH
[o] 202tHh0]x5
aidat on hatf
2KMnOy3SOy t5Cop
COoH
Kas oy+2mnsOy +3H0
+od02
Ovea0Q Hedox Heacton }
Tonic Equatbn
CmnOy+8H +5e
Coon 202 t2e|x5-
tuH03x2
oxrd aton
M nedutian ha
half
cooH
2mnOy+16Htt5007
COO
72mm +gHa0 tIbC02
ovenaAiedox Jm)
Teacher's Signatur
Calcul ation > T lea
TtS
te
tHomthe
that
balanced
2maleg o KmnOy
chemical cdox
Solution
eactton
ieats uoth 5 is cleo.
molec
Oxalic actd
MkmnOy X VkMOy
2
Moxali acrd Voxalic aticl
Remarkss.
*** *****
Page No.
Date ***
Requbtemnt>
tPpaatus
Bunette20m pipelfe, ditratfon lask
buxette watch q.lass box, chemtcal balance
werght
250mJ measuihg task.
Chemical >
Oxaltc acd cystals kmnOy solutton LOhC
Hsoy
Tndiat01 kmn04. sa sett tndfcator
End point
Obsesivation
Waght o uoatch atass 4-2qm
Wetght
lerght
ooatch qlass oxaltt
oxaltc actd 0-63
t
m actd =-83 m
Nolume oxalt acrd solution pacpared 2Bom
molanitu ooalt atd Solution =M]5o
Nolume Oxallt acid Salurtton taken for each 4itration
20m&
SNO Intfal Reading
buette Ri)
final eadng volume. ol kmoO+
byne te Ra) Soluton üsed LRa-R)
O 0mmO 6-0 me T6me
O-0 160mO0 6m
3 00 m0 16. ml o T6 m0
:0 me 16:2
Concoidant volumel6 m
16-2 nL
Teacher's Signature
Resutt 7 The molanity untnoLon kmn04 Solutfon is
Mloo&
Rgtiength is 58qm1uitre
Page
No. . Date. 2\...
EXPERIMENT -G
Aim
louoe S
To Sepaate the
and eaves
colouy component n
the ven
by the ascending paPer
ven
chromatoqHaph deteimihe ther
value
Thco
ufces contains a numbe ooHgantc
tlouoe
Compounds ohich ane Solubleun ongan solvents
64Sin ohat mass itte pape di{{enent components
the louoes 1urces can be sepanated
utces due to
theh dízioient capfllay adjon
2 4. |Yellow
GtHeen
flower
lcaves
otiginal Jina
62
6.5
2
tha onqiral lno
2
Calculatfon
R yetoe jleuoen)
O-o5
1-2
Remarks
No.
Page
Date.... sososspt|
ResulH
he colou o
Aeaves ac
pnesert in the aiven louoO t
yellous qeen ? thetr RE Valyu
Precation
.Spot dHoPped o ofaina Jine should by fch is fouo
ufce
2 snould not n mobik phase
Teacher's Signature..
Page
No. .Aim
EXPERIMmENT-7 #
Date 29-2
Sepaate. t 4
cazt fons prulent
fh 21en in
b
miture. by 4sing ascendfng pape chromatogaaph
duteuminant theår valuoR
Theoy
ooex uÍte contains a mym bey OngaTfe
COmpounds onich ane soluble in ettr ogn1
componernt
solventsBy ohat massHITe pape dilenent
to their
louoe uice, can be sepanaTed due
deent capilla14 aaion
Reauinement tppayatus
lhdtmam Jitex papeH, qlass a Aass rod
capillany tube þentlšale
chemfcal Cu cd2salt
atd
Solutfon. autone
NHyOH Solution Rubantc SPray eaqett-
* Obscvation
5-3
0 15
Resut
mittume of galt
The colout paesent îm the given_m
oLon qelloo ct 4 cdt fons tho RE
4
value ae o.154 O84
P21ecaution
dts
:Spotdroppedon Osufanal Jine should be arch
etoer ufo
Qutau
Page
No.
2
ExPERIMENT-8 # Date.23
Aim
To study Some simple aest ocapbohydjate
poteinS fat% ún Pe om
Theoi
CanbohydadtesThese are polyhydrox aldehyde or
polyhyaroxy ketone o Substances uohth on hydrolyaL
qves Such compounds
Eramples 77 Gucose, lactose -fructose. CReducin
Suga) sucrose -
(Non neducfn
Lipids hae ae
tfesten long chain
htghe atty acfds alceral. oils contatns large
pHopoAtton o
uhsgtupated acd xadicals oheuasat
cortdins saturated datty acid adicals
Examples Negetable ahee cocout oL
-ol etc
aroundnut-
Reauitement-
Appauatus Test dtubes test tubé stand q ass
10d Bunsun butnet stand
chemicalS Ag pe tha soaytÍS(ement oeath de
Page No. Date ************************
Solution
test
test
a9tbohydrateš
tubè 4 add molíschs
(ha sat formed
Catbo hydrala
paesent
1c0g ent
NOw add ima
:
d- naphthal solutfon
o
onc. HaSoy
junchon
two layers
.
tube in botltng uodter both
or o m{nuteB i
on heatng
chlotootm |Sample Afat
d mdble Preset
Test forPioteins
Xanthop2oteic test Yelloue ppt Paote'n
Take adittle oSample în atest Pxeaent
tube add
HNO 3 heat
w drops onC.
Page NO. ... . **
Date..
b)Ninhydzin test
Take Small amount sample inIntense bluo Prateth prexh
a test
nihhydrtn
tube add am o Lolour qppars
solution Bol the
mture
Btu iet let
Take 2md Sarmple Blue olet Protetn
aboutt
Say Solution o e albumnJn Lolout appeatsresent
atest add 2md oNaOH
tube
solutton.Noo add feo
drops
CusouSolutfon
Page No. Date .ML9:2)...
EXPERTMENT-9
Aim
To delec the piesene ot cabohydates fa
pstetns in the qiven food stuifsq1aes
potatoeS , >t{ce butte milk 2:10undnut
'e albumm
Theoiy
Cartbohydiate > iese ae polythydrox aldehydeo
Polyhydroxy KeTone o>1 Subsqme wht on unsgturdton
gve3uch compoundS
E lucoseLactose Aructose sucros e
Lipfds Thece ae tiiestes long chain htahe
ad ty actds4 alycerol ols contain la1ge p1opottfonn
unsat uated acfd adic als uheeas fats contain
Satated datty acid adtals
Eg Negetable ghee coconut o
PsteinsThese ane complex nitrogencous hígh molg
mass compounds These ane long ehain polymers o
d-aamno acids Paoteins aneéssenllal forqTooth
maintanance bod
Requiemerts
Appaxat us TesT+ube Test tube stand glasss
od bunsen bumer
chemicalS As Pe iequittment/o eah test
Teacher's Signature.
Page No.
Date
|
Casbonydráles tats põleins ate disussed in
Obsevatton Table
S-No- or (arbohydandes
tood stuff
orPotei=
fehlit Tollens moltsch sLodina iureT|Ninhyd
Test Test Test Teat Tat Test
2
3-
Rrce
Butte
mik
V
Groundnut
5 Ea albumin
6 UHapesS V
Potatoe
N Date
urdnut
Eggnín
Result
@ha
Chaclo
Page No.
Expe ment-|6
Objed To detet Date..1121
*** ***
S-NO Expetiment
obsetvatfong
Este Icst Tnzetence
Take iml o
ompound 4add Pint or Yed colouT
euo drops ot cedcammonfumaPpeap
Atcoholic
Pxesent
P
nitrate sd" shake
Sodfum tet
im na P
Take
,
ocompound
dest tube add 1gm
ssaen
Teacher's Signature.
**a