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SOP For Calibration of High Performance Thin Layer Chromatography (HPTLC)
SOP For Calibration of High Performance Thin Layer Chromatography (HPTLC)
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OBJECTIVE
SCOPE
RESPONSIBILITY
ACCOUNTABILITY
PROCEDURE
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1. Apply 2 μl, 4μl, 6μl, 8μl and 10 μl of solution on HPTLC plate with spotter.
2. Allow the plate to run in mobile phase.
3. Dry the plate with drier.
4. Scan the plate with scanner.
5. Check the linearity and corelation coefficient.
6. Fill in the Data as mentioned in Annexure-I
Reproducibility of Spotting
ABBREVIATIONS
%= Percentage
μl = Microlitter
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ANNEXURE
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REVISION HISTORY
Nil
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