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BIOLOGY 30 DAYS CHALLENGE

DAY 08

SRI KALAIMAGAL VID. MAT. HR. SEC. SCHOOL - PUDUVAYAL

DATE:09/05/2021
BIOLOGY MARK- 35
STD: XII BIO-BOTANY TIME-1.30 hrs

Principles and Processes of Biotechnology

PART A
I. Choose The Correct Answer: 08x01=08
1. Plasmids are
a. circular protein molecules b. required by bacteria
c. tiny bacteria d. confer resistance to antibiotics
2. pBR 322, BR stands for
a. Plasmid Bacterial Recombination b. Plasmid Bacterial Replication
c. Plasmid Boliver and Rodriguez d. Plasmid Baltimore and Rodriguez
3. In which techniques Ethidium Bromide is used?
a. Southern Blotting techniques b. Western Blotting techniques
c. Polymerase Chain Reaction d. Agrose Gel Electroporosis
4. An analysis of chromosomal DNA using the southern hybridisation technique does not use
a) Electrophoresis b) Blotting c) Autoradiography d) Polymerase Chain Reaction
5. What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
a) The smaller the fragment size, the farther it moves.
b) Positively charged fragments move to farther end.
c) Negatively charged fragments do not move.
d) The larger the fragment size, the farther it moves.
6. For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up
of
a) Silver or platinum b) Platinum or zinc
c) Silicon or platinum d) Gold or tungsten.
7. Which vector can clone only a small fragment of DNA?
a) Bacterial artificial chromosome b) Yeast artificial chromosome c) Plasmid d) Cosmid
8. Ligase is used for
a) Joining of two DNA fragments b) Separating DNA
c) DNA polymerase reaction d) All of these
 PART B
II. Answer Any Four Of The Following Questions: 04x02=08
9. What are the materials used to grow microorganism like Spirulina?
10. Name the chemicals used in gene transfer.
11. How will you identify vectors?
12. What is gene knock out?
13. Name the methods of Gene transfer.
14. write a short note on Alkaline phosphate.

PART C
III. Answer Any Three Of The Following: 03x03=09
15. What do you know about the word pBR332 and explain?
16. Compare the various types of Blotting techniques
17. Write the advantages and disadvantages of Bt cotton.
18. List out the application of SCP.
19. What is bioremediation? Give an example.

PART D
IV. Answer the following questions in details: 02x05=10
20. Write the application of fermentation industry?
Or
Explain the process of Agarose GEL Electrophoresis.
21. a) What is bioremediation? give some examples of bioremediation.
b) Write the benefits and risk of Genetically Modified Foods.
Or
What are the restriction enzymes? Mention their role in biotechnology.

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Question prepared by
A.VINOTH KUMAR. DGD., M.Sc.,M.Phil.,B.Ed .
PG.ASST. IN BIOLOGY,
SRI KALAIMAGAL VID.MATRIC.HR.SEC.SCHOOL
PUDHUVAYAL.
CELL.6369060979