MOLISCH’S TEST SCHIFF’S TEST

1. In 3 test tubes place 3 mL of Sucrose, Glucose and Lactose

1. Place 3mL of the solutions in 3
EXPERIMENT 2 solution
2. Add 3 drops of Molisch’s reagent to each. Shake the solution,
incline each tubes and slowly add 3 mL of concentrated separate test tubes
MONOSACCHARIDE & sulfuric acid. DO NOT SHAKE.
3. Carefully put tubes upright and note color of ring formed at
2. Add 1mL of Schiff’s reagent into
DISACCHARIDE the junction of two liquids
4. Compare the results in each test tubes.
each tubes and note for color
change
3. Record results

REDUCING PROPERTIES FEHLING’S TEST BENEDICT’S TEST

TOLLEN’S TEST
1.Place 2mL of the test solutions in 3 separate
tubes and add 3mL of ammoniacal silver
nitrate in each.
2.Shake tubes gently then warm in water bath.
3.Observe for changes
1. Place 2mL of test solutions in separate tubes. Add
3mL of Fehling’s solution (Fehling’s A and Fehling’s
B combined in equal ratio) in each tubes.
2. Heat test tubes in water bath
3. Note reaction and color change giving attention
for any precipitate formed at the bottom
1. Place 3mL of test solutions in separate tubes.
Add 3mL of Benedict’s solution in each
2. Warm in water bath
3. Observe the reactions and record results.

MOORE’S TEST PICRIC ACID TEST NYLANDER’S TEST

1. Place 2 mL of test solutions in separate
tubes
2. Add 3 mL strong NaOH in each and boil
for 3-5 minutes
3. Note for changes in each
1. In separate tubes place 2mL of test • Place 2mL test solutions in separate
solutions. tubes
2. Add 2 mL picric acid and 0.5 gram • Add 1mL of Nylander’s reagent in each
Sodium carbonate and heat tubes in water bath
3. Warm test tubes in water bath • Note changes in test tubes.
4. Observe the color change