CÂU HỎI ÔN TẬP

Câu 1. In atomic absorption spectrophotometry (AAS):

A. lemission = lexcitation
B. lemission < lexcitation
C. lemission > lexcitation
D. lemission ≥ lexcitation
Câu 2. In atomic emission spectrophotometry (AES):
A. lemission = lexcitation
B. lemission < lexcitation
C. lemission > lexcitation
D. lemission ≥ lexcitation
Câu 3. An atomic emission spectrometer operates using which principle?
A. Molecular absorption spectrophotometry
B. Molecular emission spectrophotometry
C. Atomic absorption spectrophotometry
D. Atomic emission spectrophotometry
Câu 4. The hollow cathode lamp consists of a tungsten anode and a cylindrical cathode which is constructed of the
metal
A. Au
B. Fe
C. Hg
D. Whose spectrum is desired or serves to support a layer of that metal
Câu 5. The quantitative methods of analysis for elements in atomic spectrophotometry are:
A. Atomic absorption spectrophotometry
B. Atomic emission spectrophotometry
C. Plasma emission
D. All are correct
Câu 6. For Atomic absorption spectrophotometry quantitative assay of calcium, which wavelength is suitable
A. 766 nm
B. 422.7 nm
C. 589 nm
D. 671 nm
Câu 7. For purposes of comparison, what is the major advantage of atomic emission spectrophotometry (AES)
against atomic adsorption spectrophotometry (AAS)?
A. In AES method, the flame serves as the source
B. AES requires an individual lamp for each element
C. Detection limit of all element is always better
 D. In the hands of a skilled operator, uncertainties are better for AES procedure
Câu 8. The liquid junction potential is:
A. Derived from the difference in ion mobility between two test solutions
B. Derived from the difference in ion mobility between two electrolyte solutions
C. Developped on the interface between metal and its salty solution where that metal immersed
D. Generated by thermal agitation of solutes
Câu 9. The indicator electrode used for acid-base titration is:
A. Silver electrode
B. Ion selective electrode
C. Glass electrode
D. Platinum electrode
Câu 10. For pH measurements, glass electrode can be replaced by:
A. Silver/Silver chloride electrode
B. Gas-sensing probe
C. Hydrogen electrode
D. All are correct
Câu 11. What is the indicator electrode in redox titration?
A. Silver electrode
B. Ion Selective Electrode
C. Glass electrode
D. Platinum electrode
Câu 12. In acid-base titration, the determination of equivalent point is based on:
A. The jump of pH of titrand during titration
B. The jump of voltage of titrand during titration
C. The jump of intensity of diffuse current during titration
D. Sudden change of intensity of diffuse current during titration
Câu 13. Technique for separation of heterogenous mixture is:
A. Filtration
B. Distillation
C. Chromatography
D. Extraction
Câu 14. Phase transition is:
A. Transition of a one phase into two phases
B. Transition of a liquid substance into gaseous substance
C. Transition of a substance from this phase into another phase
D. Transition of a solid substance into gaseous substance
Câu 15. Technique for separation of homogenous mixture is:
A. Centrifugation
B. Filtration
C. Phase transition
D. Handpicking
Câu 16. Gas chromatography is a method for separation:
A. Mixtures of gas using a close tubular column
B. Mixtures of volatile compounds using an open tubular column
C. Volatile compounds at a chromatographic temperature on close tubular column
D. Gaseous compounds
Câu 17. In gas chromatography, mobile phase:
A. Plays an decisive role for separation
B. Interacts with analytes in order to obtain a good looking chromatogram
C. Only carries analytes throughout the column
D. Is a liquid which is vaporized, migrated throughout the stationary phase in a specific direction
Câu 18. In gas chromatography, which carrier gas is the most commonly used:
A. Nitrogen
B. Hydrogen
C. Inert gas
D. Air
Câu 19. A wall coated open tubular is an open capillary column in which……is chemically bonded with the interior
surface of the capillary
A. Liquid stationary phase
B. Solid stationary phase
C. Solid support
D. Solid support coated with liquid stationary phase
Câu 20. In gas chromatography, the column temperature should be:
A. High enough to maintain the sample in a gaseous phase and equal to the detector temperature
B. Equal to the detector temperature but not to damage the stationary phase
C. High enough to maintain the sample in a gaseous phase but not to damage the stationary phase
D. Equal to the injection and the detector temperature
Câu 21. The absorption of a molecule in the UV-Vis region may indicate:
A. Molecular formula
B. Structure determination
C. Number of carbon atom
D. Number of hydrogen atom
Câu 22. Radiation in the UV-Vis region is divided into:
 A. Far UV region
B. Near UV region
C. Visible region
D. All are correct
Câu 23. The   * transition requires………energy than   * transition
A. Less
B. More
C. Much less
D. Much more
Câu 24. The Woodward Rules predicts the base value for maximal adsorption wavelength of heteroannular diene
as:
A. 214 nm
B. 217 nm
C. 254 nm
D. 365 nm
Câu 25. Capillary electrophoresis is an analytical technique that separates ions based on their: 
A. Electrophoretic mobility
B. Electroosmotic flow
C. Migration time
D. Column efficiency
Câu 26. Electrophoretic mobility is affected by:
A. Charge of solute, size of solute
B. The viscocity of the analytes
C. The length of the column
D. The sensitivity of the detector
Câu 27. The basic difference between gel elcreophoresis and capillary electrophoresis is:
A. No support is needed
B. There exists a Joule heating
C. The electroosmotic flow is formed
D. The applied voltage is up to 30 kV
Câu 28. The electroosmotic flow:
A. Is formed due to the buffer solution containing ions
B. Bears positive charges
C. Is formed due to the formation of negatively charged silica-surface
D. Is formed due to the presence of zeta potential
Câu 29. The disadvantage of Capillary Zone Electrophoresis is that it cannot separate
A. Weak acids
 B. Weak bases
C. Neutral analytes
D. Mixture of cation, anion and neutral analyte
Câu 30. In capillary electrophoresis, the capillary column efficiency is predicted by:
A. Rf
B. Capacity factor
C. Resolution
D. Number of theoretical plate
Câu 31. Micellar Electrokinetic Chromatography
A. Being able to separate a mixture of cation, anion and neutral analyte
B. The mode of separation is based on electrophoretic mobility of analyte
C. Using micelle as a pseudo-stationary phase
D. All are correct
Câu 32. Which adsorbent are most commonly used in thin layer chromatography?
A. Cellulose, aluminum oxide
B. Sephadex, active charcoal
C. Polyamide, active charcoal
D. Silica gel, aluminum oxide
Câu 33. Thin layer chromatography is an analytical method in which:
A. Mobile phase is a liquid phase, stationary phase is a solid phase
B. Stationary phase is spread as a thin layer in a glass, metal or plastic plate
C. Mobile phase flows as a thin layer throughout the support
D. Stationary and solid phase are liquid phase
Câu 34. Cellulose as stationary phase in thin layer chromatography for separation the mixture using which mode?
A. Adsorption
B. Partition
C. Size-exclusion
D. Ion-exchange
Câu 35. High Performance Thin Layer Chromatography (HPTLC) plate is different from traditional Thin Layer
Chromatography (TLC) plate at:
A. The size of the plate is bigger, the thickness of the stationary phase is thinner
B. The particle size of stationary phase is smaller, the thickness of the stationary phase is thinner
C. The particle size of stationary phase is bigger, the thickness of the stationary phase is thicker
D. The size of the plate is smaller, the thickness of the stationary phase is smaller
Câu 36. In quality control, thin layer chromatography is used for
A. For identification of organic compounds only
B. For quantitative assay of organic compounds only
 C. For identification, semi-quantitative assay and determination of related substances
D. For identification and quantitative assay of organic compounds
Câu 37. The number of theoretical plate of a chromatographic column is:
A. The number of continuous countercurrent extraction
B. The parameter need to change for separation of multi-component
C. The parameter for prediction of the separation power of the column with a specific compound
D. The parameter for prediction of a dynamic and kinetics process that occur in the column
Câu 38. The stationary is:……in the chromatographic system
A. A mobile phase
B. A non-mobile phase
C. A gaseous phase
D. The most important phase
Câu 39. The symmetry factor (Tailing factor) of a peak for a quantitative assay:
A. 0.5 ≤ T < 0.8
B. T = 0.8
C. 1.0 ≤ T < 1.5
D. 0.8 ≤ T < 1.2
Câu 40. In chromatography, the mobile phase are:
A. Gas, liquid or supercritical fluid
B. Always liquid
C. Always gaseous
D. Sometimes solid
Câu 41. The ion-exchange mode in chromatographic method is the separation of analytes based on:
A. Size of their ion molecule
B. The exchange of counter ion between solute ion and stationary phase
C. The exchange of ion between solute ion and stationary phase
D. Their ion dissociation property
Câu 42. Which parameter is the most important one for a quantitative assay of two components by chromatography
A. Number of theoretical plate
B. Symmetry factor
C. Resolution
D. Retention time
Câu 43. Infrared spectroscopy is:
A. Vibrational rotational spectroscopy
B. Electronic spectroscopy
C. Molecular spectroscopy
 D. Atomic spectroscopy
Câu 44. Which infrared region used in drug quality control
A.  = 375 – 1100 nm
B.  =1100 nm – 2500 nm or = 9090 - 4000 cm-1
C.  =2500 nm – 25000 nm or = 4000 - 400 cm-1
D. All are incorrect
Câu 45. Which unit represents the wavenumber in infrared spectroscopy:
A. nm
B. s-1
C. cm-1
D. cm
Câu 46. The infrared spectrophotometry method can measure:
A. Solid sample in KBr
B. Gaseous sample
C. Liquid sample in solution
D. All are correct
Câu 47. What type of vibrations in infrared spectrophotometry?
A. Stretching and Bending
B. Stretching and Rocking
C. Stretching and Twisting
D. Stretching and Wagging
Câu 48. Theoretically, water (H2O) that adsorps radiation in the infrared region has how many fundamental
vibrations?
A. 3
B. 4
C. 5
D. 6
Câu 49. In the electrochemical cell, the reduction reaction occurs in which half-cell?
A. Anode
B. Cathode
C. Indicator electrode
D. Double electrode
Câu 50. Which statement related to Karl Fischer titration is NOT correct?
A. Basing on the reaction of: I2 + SO2 + H2O = 2HI + SO3
B. Being able to determine water content in solvent
C. Being able to determine water content in drug substance
D. Being able to determine water content in drug product
Câu 51. HPLC is a chromatographic technique for:
A. Separation of a mixture using the column packed with anionic resin
B. Separation of a mixture using the column packed with cationic resin
C. Separation of a mixture using the capillary column
D. Separation of a mixture using the column packed with ≤ 10 mm particle size
Câu 52. In order to inject sample onto the column, one can perform as the following:
A. Using the syringe for direct injection of sample onto the column
B. Using the autosampler
C. Using the loop
D. All are correct
Câu 53. In liquid chromatography, what is normal-phase mode
A. Polar stationary phase and non-polar mobile phase
B. Polar stationary phase and polar mobile phase
C. Non-polar stationary phase and non-polar mobile phase
D. Non-Polar stationary phase and polar mobile phase
Câu 54. In liquid chromatography, what is reversed-phase mode
A. Polar stationary phase and non-polar mobile phase
B. Polar stationary phase and polar mobile phase
C. Non-polar stationary phase and non-polar mobile phase
D. Non-Polar stationary phase and polar mobile phase
Câu 55. In liquid chromatography, for identification, one can refer to:
A. Rf and Rs
B. Retention time
C. Capacity factor
D. Peak area or peak height
Câu 56. In liquid chromatography, for quantitative assay, one can refer to:
A. Rf and Rs
B. Retention time
C. Symmetric factor
D. Peak area or peak height
Câu 57. In spectrophotometry, energy of any radiation is in inverse ratio to:
A. Frequency
B. Cycle
C. Number of vibration
D. Wavelength
Câu 58. The diffraction and interference are used to explain which nature of light?
 A. Wave
B. Particle
C. Vibration
D. Rotation
Câu 59. Visible beam, UV beam, infrared beam … are different forms of electromagnetic radiation. They are only
different about:
A. Transmittance
B. Absorbance
C. Energy
D. Wavelength
Câu 60. What is the value of Planck’s constant?
A. 6.626 x 10-34 J s
B. 6.626 x 10-34 erg/sec
C. 6.626 x 10-27 J s
D. 6.626 x 10-27 erg/sec
Câu 61. The electromagnetic radiation as particles in a bundle of light energy called
A. Photon
B. Electron
C. Proton
D. Neutron
Câu 62. The diffraction and interference are used to explain which nature of light?
A. Wave
B. Particle
C. Vibration
D. Rotation
Câu 63. The hollow cathode lamp consists of a tungsten anode and a cylindrical cathode which is constructed of the
metal
A. Au
B. Fe
C. Hg
D. Whose spectrum is desired or serves to support a layer of that metal
Câu 64. In atomic emission spectrophotometry (AES):
A. lemission = lexcitation
B. lemission < lexcitation
C. lemission > lexcitation
D. lemission ≥ lexcitation
Câu 65. An atomic emission spectrometer operates using which principle?
 A. Molecular absorption spectrophotometry
B. Molecular emission spectrophotometry
C. Atomic absorption spectrophotometry
D. Atomic emission spectrophotometry
Câu 66. The quantitative methods of analysis for elements in atomic spectrophotometry are:
A. Atomic absorption spectrophotometry
B. Atomic emission spectrophotometry
C. Plasma emission
D. All are correct
Câu 67. For Atomic absorption spectrophotometry quantitative assay of calcium, which wavelength is suitable
A. 766 nm
B. 422.7 nm
C. 589 nm
D. 671 nm
Câu 68. For purposes of comparison, what is the major advantage of atomic emission spectrophotometry (AES)
against atomic adsorption spectrophotometry (AAS)?
A. In AES method, the flame serves as the source
B. AES requires an individual lamp for each element
C. Detection limit of all element is always better
D. In the hands of a skilled operator, uncertainties are better for AES procedure
Câu 69. The liquid junction potential is:
A. Derived from the difference in ion mobility between two test solutions
B. Derived from the difference in ion mobility between two electrolyte solutions
C. Developped on the interface between metal and its salty solution where that metal immersed
D. Generated by thermal agitation of solutes
Câu 70. The indicator electrode used for precipitation titration is:
A. Silver electrode
B. Ion selective electrode
C. Glass electrode
D. Platinum electrode
Câu 71. For pH measurements, glass electrode can be replaced by:
A. Silver/Silver chloride electrode
B. Gas-sensing probe
C. Hydrogen electrode
D. All are correct
Câu 72. What is the indicator electrode in complexation titration?
A. Silver electrode
 B. Ion Selective Electrode
C. Metallic indicator electrode
D. Platinum electrode
Câu 73. In acid-base titration, the determination of equivalent point is based on:
A. The jump of pH of titrand during titration
B. The jump of voltage of titrand during titration
C. The jump of intensity of diffuse current during titration
D. Sudden change of intensity of diffuse current during titration
Câu 74. Technique for separation of heterogenous mixture is:
A. Filtration
B. Distillation
C. Chromatography
D. Extraction
Câu 75. The apparent partition coefficient depends on:
A. Temperature
B. Pressure
C. pH
D. Solvent
Câu 76. Technique for separation of homogenous mixture is:
A. Centrifugation
B. Filtration
C. Phase transition
D. Handpicking
Câu 77. Structure of a gas chromatograph includes sequentially:
A. Gas system, injection port, column/column oven, detector, data processor
B. Gas system, column/column oven, injection port , detector, data processor
C. Gas system, detector, injection port, column/column oven, data processor
D. Injection port, gas system, column/column oven, detector, data processor
Câu 78. In gas chromatography, for separation of polar mixture, which stationary phase should be used:
A. Diethylene glycol adipate
B. Polyehylene glycol
C. Diethylene glycol succinate
D. Methyl silicone polymers 
Câu 79. Which factor most influence the extraction and spectrophotometry method for quality control?
A. Ion pair agent
B. pH
 C. Extraction time
D. Solvent
Câu 80. A wall coated open tubular is an open capillary column in which……is chemically bonded with the interior
surface of the capillary
A. Liquid stationary phase
B. Solid stationary phase
C. Solid support
D. Solid support coated with liquid stationary phase
Câu 81. In solvent extraction of metal chelates: the formation of………between metal ion and a ligand is used
A. Precipitation
B. Complex
C. Ion pair
D. Double salt
Câu 82. The absorption of a molecule in the UV-Vis region may indicate:
A. Molecular formula
B. Structure determination
C. Number of carbon atom
D. Number of hydrogen atom
Câu 83. Radiation in the UV-Vis region is divided into:
A. Far UV region
B. Near UV region
C. Visible region
D. All are correct
Câu 84. The   * transition requires………energy than   * transition
A. Less
B. More
C. Much less
D. Much more
Câu 85. The Woodward Rules predicts the base value for maximal adsorption wavelength of heteroannular diene as:
A. 214 nm
B. 217 nm
C. 254 nm
D. 365 nm
Câu 86. Capillary electrophoresis is an analytical technique that separates ions based on their: 
A. Electrophoretic mobility
B. Electroosmotic flow
C. Migration time
 D. Column efficiency
Câu 87. Electrophoretic mobility is affected by:
A. Charge of solute, size of solute
B. The viscocity of the analytes
C. The length of the column
D. The sensitivity of the detector
Câu 88. The basic difference between gel elcreophoresis and capillary electrophoresis is:
A. No support is needed
B. There exists a Joule heating
C. The electroosmotic flow is formed
D. The applied voltage is up to 30 kV
Câu 89. The electroosmotic flow:
A. Is formed due to the buffer solution containing ions
B. Bears positive charges
C. Is formed due to the formation of negatively charged silica-surface
D. Is formed due to the presence of zeta potential
Câu 90. The disadvantage of Capillary Zone Electrophoresis is that it cannot separate
A. Weak acids
B. Weak bases
C. Neutral analytes
D. Mixture of cation, anion and neutral analyte
Câu 91. In capillary electrophoresis, the capillary column efficiency is predicted by:
A. Rf
B. Capacity factor
C. Resolution
D. Number of theoretical plate
Câu 92. Micellar Electrokinetic Chromatography
A. Being able to separate a mixture of cation, anion and neutral analyte
B. The mode of separation is based on electrophoretic mobility of analyte
C. Using micelle as a pseudo-stationary phase
D. All are correct
Câu 93. Which adsorbent are most commonly used in thin layer chromatography?
A. Cellulose, aluminum oxide
B. Sephadex, active charcoal
C. Polyamide, active charcoal
D. Silica gel, aluminum oxide
Câu 94. TLC plate efficiency is predicted by:
A. Capacity factor
B. Retardation factor
C. Number of theoretical plate
D. Resolution
Câu 95. Cellulose as stationary phase in thin layer chromatography for separation the mixture using which mode?
A. Adsorption
B. Partition
C. Size-exclusion
D. Ion-exchange
Câu 96. High Performance Thin Layer Chromatography (HPTLC) plate is different from traditional Thin Layer
Chromatography (TLC) plate at:
A. The size of the plate is bigger, the thickness of the stationary phase is thinner
B. The particle size of stationary phase is smaller, the thickness of the stationary phase is thinner
C. The particle size of stationary phase is bigger, the thickness of the stationary phase is thicker
D. The size of the plate is smaller, the thickness of the stationary phase is smaller
Câu 97. In quality control, thin layer chromatography is used for
A. For identification of organic compounds only
B. For quantitative assay of organic compounds only
C. For identification, semi-quantitative assay and determination of related substances
D. For identification and quantitative assay of organic compounds
Câu 98. The number of theoretical plate of a chromatographic column is:
A. The number of continuous countercurrent extraction
B. The parameter need to change for separation of multi-component
C. The parameter for prediction of the separation power of the column with a specific compound
D. The parameter for prediction of a dynamic and kinetics process that occur in the column
Câu 99. The stationary is:……in the chromatographic system
A. A mobile phase
B. A non-mobile phase
C. A gaseous phase
D. The most important phase
Câu 100. The symmetry factor (Tailing factor) of a peak for a quantitative assay:
A. 0.5 ≤ T < 0.8
B. T = 0.8
C. 1.0 ≤ T < 1.5
D. 0.8 ≤ T < 1.2
Câu 101. In chromatography, the mobile phase are:
 A. Gas, liquid or supercritical fluid
B. Always liquid
C. Always gaseous
D. Sometimes solid
Câu 102. The ion-exchange mode in chromatographic method is the separation of analytes based on:
A. Size of their ion molecule
B. The exchange of counter ion between solute ion and stationary phase
C. The exchange of ion between solute ion and stationary phase
D. Their ion dissociation property
Câu 103. Which parameter is the most important one for a quantitative assay of two components by chromatography
A. Number of theoretical plate
B. Symmetry factor
C. Resolution
D. Retention time
Câu 104. Infrared spectroscopy is:
A. Vibrational rotational spectroscopy
B. Electronic spectroscopy
C. Molecular spectroscopy
D. Atomic spectroscopy
Câu 105. Which infrared region used in drug quality control
A.  = 375 – 1100 nm
B.  =1100 nm – 2500 nm or = 9090 - 4000 cm-1
C.  =2500 nm – 25000 nm or = 4000 - 400 cm-1
D. All are incorrect
Câu 106. Which unit represents the wavenumber in infrared spectroscopy:
A. nm
B. s-1
C. cm-1
D. cm
Câu 107. The infrared spectrophotometry method can measure:
A. Solid sample in KBr
B. Gaseous sample
C. Liquid sample in solution
D. All are correct
Câu 108. What type of vibrations in infrared spectrophotometry?
A. Stretching and Bending
 B. Stretching and Rocking
C. Stretching and Twisting
D. Stretching and Wagging
Câu 109. Theoretically, paracetamol (C8H9NO2) that adsorps radiation in the infrared region has how many
fundamental vibrations?
A. 44
B. 34
C. 24
D. 54
Câu 110. What is the electrode potential of hydrogen electrode at 25 oC, PH2 = 1 atm
A. 0,000 V
B. 0,59 V
C. - 0,59 V
D. -0,059 V
Câu 111. Glass Electrode is a:
A. Metallic Indicator Electrode
B. Ion selective membrane indicator electrode
C. Reference electrode
D. Crystalline membrane electrode
Câu 112. The absorption of radiation energy……make(s) an increase in mass of adsorbent
A. Cannot
B. Can
C. May
D. Definitely
Câu 113. In order to inject sample onto the column, one can perform as the following:
A. Using the syringe for direct injection of sample onto the column
B. Using the autosampler
C. Using the loop
D. All are correct
Câu 114. In liquid chromatography, what is normal-phase mode
A. Polar stationary phase and non-polar mobile phase
B. Polar stationary phase and polar mobile phase
C. Non-polar stationary phase and non-polar mobile phase
D. Non-Polar stationary phase and polar mobile phase
Câu 115. In liquid chromatography, what is reversed-phase mode
A. Polar stationary phase and non-polar mobile phase
B. Polar stationary phase and polar mobile phase
 C. Non-polar stationary phase and non-polar mobile phase
D. Non-Polar stationary phase and polar mobile phase
Câu 116. In liquid chromatography, for identification, one can refer to:
A. Rf and Rs
B. Retention time
C. Capacity factor
D. Peak area or peak height
Câu 117. In spectrophotometry, energy of any radiation is in inverse ratio to:
A. Frequency
B. Cycle
C. Number of vibration
D. Wavelength
Câu 118. The molecule or ion absorbed radiation to produce some type of transtion including:
A. Electronic, radiative, rotational transition
B. Electronic, vibrational, rotational, translational transition
C. Electronic, emission, rotational transition
D. Radiative, vibrational, rotational transition
Câu 119. For Atomic absorption spectrophotometry quantitative assay of magnesium, which wavelength is suitable
A. 285.2 nm
B. 422 nm
C. 589 nm
D. 671 nm
Câu 120. Visible beam, UV beam, Infrared beam … are different form of electromagnetic radiation. They are only
different about:
A. Transmittance
B. Absorbance
C. Energy
D. Wavelength