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Available online at www.sciencedirect.com

ScienceDirect

journal homepage: www.intl.elsevierhealth.com/journals/dema

Stability of endodontically treated teeth with


differently invasive restorations: Adhesive vs.
non-adhesive cusp stabilization

Roland Frankenberger a,∗ , Inka Zeilinger a , Michael Krech a , Gernot Mörig a,b ,
Michael Naumann c , Andreas Braun a , Norbert Krämer d ,
Matthias J. Roggendorf a
a Department of Operative Dentistry and Endodontics, Medical Center for Dentistry, University Medical Center
Giessen and Marburg, Campus Marburg, Georg-Voigt-Str. 3, D-35039 Marburg, Germany
b Private Practice, Düsseldorf, Germany
c Department of Prosthetic Dentistry, University of Ulm, Albert-Einstein-Allee 11, D-89081 Ulm, Germany
d Department of Pediatric Dentistry, Medical Center for Dentistry, University Medical Center Giessen and Marburg,

Campus Giessen, Schlangenzahl 14, D-35392 Giessen, Germany

a r t i c l e i n f o a b s t r a c t

Article history: Objectives. Aim of the present study was to evaluate fracture strength of endodontically
Received 11 March 2015 treated molars with different preparations/restorations after thermomechanical loading in
Received in revised form vitro.
11 August 2015 Methods. 264 extracted human third molars were used. Beside the control group, 256 teeth in
Accepted 17 August 2015 32 test groups (n = 8) received root canal treatment (MTwo #40/.6) and root canal obturation
Available online xxx with AH Plus and Guttapercha. After postendodontic sealing and build-up (Syntac, SDR),
specimens were additionally prepared MO or MOD. Postendodontic restorations were: Direct
Keywords: restorations (Tetric EvoCeram Bulk Fill bonded with Syntac; as filling or direct partial crown
Endodontically treated teeth (PC) after reducing the cusps 3 mm; amalgam as filling or direct pin-retained partial crown
Cuspal stabilization (PC)), vs. indirect adhesive restorations (I: Inlay vs. PC; IPS Empress I/PC; Celtra Duo I/PC;
Resin composites e.max CAD I/PC; Lava Ultimate I/PC; Enamic I/PC – all inserted with Syntac/Variolink) vs.
Resin nano ceramic cemented cast gold I/PC. After 300,000 thermocycles (5/55 ◦ C) and 1.2 Mio. 100 N load cycles,
Lithium disilicate ceramic specimens were loaded until fracture.
Leucite-reinforced glass ceramic Results. Whereas IPS Empress showed no difference between I and PC (p > 0.05), in all other
Cast gold groups PC were significantly more stable than fillings/inlays (p < 0.05), this effect was more
Amalgam pronounced after MOD preparations. Cast gold PC exhibited the highest fracture strengths
(p < 0.05), inlays the lowest (p < 0.05). IPS Empress was generally inferior to the other bonded
materials under investigation (p < 0.05) which as PC almost reached the level of control
specimens. Amalgam fillings showed the worst outcome (p < 0.05).


Corresponding author. Department of Operative Dentistry and Endodontics, Medical Center for Dentistry, University Medical Center
Giessen and Marburg, Campus Marburg, Georg-Voigt-Str. 3, D-35039 Marburg, Germany. Tel.: +49 6421 5863240; fax: +49 6421 5863745.
E-mail address: frankbg@med.uni-marburg.de (R. Frankenberger).
http://dx.doi.org/10.1016/j.dental.2015.08.160
0109-5641/© 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

Please cite this article in press as: Frankenberger R, et al. Stability of endodontically treated teeth with differently invasive restorations: Adhesive
vs. non-adhesive cusp stabilization. Dent Mater (2015), http://dx.doi.org/10.1016/j.dental.2015.08.160
DENTAL-2613; No. of Pages 9
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Significances. Less invasive preparation designs were not beneficial for the stability of pos-
tendodontic restorations. Except for IPS Empress, PC were generally more successful in
restabilization of weakened cusps after endodontic treatment and preparation. Cast gold
PC remain the ultimate stabilization tool for ETT in terms of fracture resistance.
© 2015 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.

preparation and (2) the restoration would have no influence on


1. Introduction fracture strength of previously thermomechanically fatigued
ETT.
Since decades, vital teeth can be safely restored and kept vital
using amalgam and more and more resin composites in order
to act minimally invasive [1–5]. During and especially after
completed endodontic treatment, the situation considerably
2. Methods and materials
changes due to the weakening effect of endodontic access
preparation and the often large amount of tissue having been 264 intact, non-carious, unrestored human lower third molars
lost by extensive caries excavation [6–12]. It is proven that the with similar size and fully developed roots, extracted for ther-
biomechanical stability of posterior teeth after access cavity apeutic reasons under informed consent of the patients and
and even further preparation is dramatically decreased with upon approval by a local ethics committee (Ref no. 143/09),
a high risk of vertical root fracture [13–18]. Thus, it is logical were stored in an aqueous solution of 0.5% chloramine T at
that clinical studies focusing on endodontically treated teeth 4 ◦ C for up to 30 days. The teeth were debrided of residual
(ETT) reveal inferior outcomes compared to vital teeth [19–24], plaque and calculus, and examined to ensure that they were
although recent studies could not find less tactile sensitivity free of defects under a light microscope at ×20 magnifica-
of ETT compared to vital teeth which makes the “cherry stone” tion. Beside the control group without any preparation (n = 8),
theory questionable, i.e. that the opening reflex for ETT is be 256 teeth in 32 groups (n = 8) received endodontic access cavi-
delayed, involving heavier load input during routine mastica- ties and root canal preparation at a working length of −1 mm
tion of hard items [25]. from the apical foramen using MTwo rotary instruments (VDW,
However, a deeper look into clinical data exhibits that Munich, Germany) up to size .04/#40. Tooth length was estab-
vertical root fractures accounted only for 12% of extractions lished using a C-Pilot file ISO 10 (VDW) that was inserted
of ETT, compared to 15% cusp fractures and 40% periodon- into the root canal until it could be visualized at the api-
tal problems [20]. Nevertheless, this altogether sums up to cal foramen. Working length was determined by subtracting
27% fractures as reason for ETT loss [20]. So the appropriate 1 mm from this length. Root canals were filled with laterally
preparation and/or restoration was always a matter of con- compacted gutta-percha (VDW) and AH Plus sealer (Dentsply
cern in the literature, having been reflected by several studies DeTrey, Konstanz, Germany), and immediately sealed (Syntac
and reviews with a special focus on postendodontic restora- and SDR/Dentsply).
tions [26–34]. Whereas for the restoration of vital teeth it is Specimens were additionally prepared MO or MOD. The
generally accepted that minimally invasive preparations are cavities were cut using coarse diamond burs under profuse
supporting clinical long-term success [1,4,35], the influence of water cooling (80 ␮m diamond, Komet, Lemgo, Germany), and
preparation invasiveness with ETT is still not fully understood. finished with a 25 ␮m finishing diamond (one pair of diamonds
For eample, the clinical outcome of direct resin compos- per four cavities). Inner angles of the cavities were rounded
ite restorations is discussed contradictorily in the literature and the margins were not beveled except for direct restora-
of the field ranging from simply catastrophic to acceptable tions where margins received a 0.5 mm bevel. Polymerizable
[14,20,36–41]. The same is true for the preclinical estimation materials were light-cured with a Bluephase light-curing unit
of innovative ceramics and hybrid materials, which have been (Ivoclar Vivadent, Schaan, Principality of Liechtenstein). The
claimed to act as better shock absorbers during mastication intensity of the light was checked periodically with a radiome-
which also makes them interesting as ETT restorations [28,51]. ter (Demetron Research Corp, Danbury, CT, USA) to ensure that
Although prospective, randomized clinical long-term trials are 1000 mW/cm2 was always delivered during the experiments.
the optimum instrument to investigate clinical behavior of The different restorative procedures are displayed in
different restorative procedures in vivo, these studies are time- Table 1. Direct adhesive procedures: cavities (MO, MOD, par-
consuming, expensive, and permanently suffering potential tial crown preparations, Figs. 1 and 2) were surrounded with a
patient drop out which is finally corroborating statistical metal matrix band, bonded with Syntac, and restored with Tet-
impact [3,35]. Therefore, in vitro studies are still of concern, ric EvoCeram Bulk Fill in oblique layers of 2–4 mm thickness.
especially when thermomechanical loading scenarios are The increments were separately light-cured for 40 s each with
applied being close to the clinically observed circumstances the light source in contact with the coronal edge of the matrix
[7,8,42,43]. band. After removal of the matrix band, the restorations were
Aim of the present study was to evaluate the fracture light-cured from their buccal and lingual aspects for an addi-
resistance and behavior of different preparation/restoration tional 20 s on each side. Prior to the finishing process, visible
concepts of ETT. The null hypothesis was two-fold, that (1) the overhangs were removed using a posterior scaler (A8 S204S,

Please cite this article in press as: Frankenberger R, et al. Stability of endodontically treated teeth with differently invasive restorations: Adhesive
vs. non-adhesive cusp stabilization. Dent Mater (2015), http://dx.doi.org/10.1016/j.dental.2015.08.160
DENTAL-2613; No. of Pages 9
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Table 1 – Experimental set up of the study.

Abbreviations: F: filling, DPC: direct partial crown, I: inlay, PC: partial crown, RC: resin composite, IP: IPS Empess, EM: e.max CAD, CD: Celtra
Duo, LU: Lava Ultimate, EN: Enamic, CG: Cast gold, AM: Amalgam, MO: additional MO preparation, and MOD: additional MOD preparation.

Hu-Friedy, Leimen, Germany). Proximal margins were finished consecutive layers. In partial crown situations, one para-
with flexible disks (SofLex Pop-on, 3M ESPE, St. Paul, USA). pulpal pin was applied on each replaced cusp (Edenta, Au,
Direct amalgam procedures: cavities (MO, MOD, partial Switzerland). Amalgam restorations were polished after 24 h.
crown preparations with flattened cusps) were surrounded Indirect adhesive procedures (I: Inlay vs. PC) were IPS
with a metal matrix band, and amalgam (Dispealloy, Empress CAD I/PC and e.max CAD I/PC (Ivoclar Vivadent),
Dentsply, Konstanz, Germany) was applied with a syringe in Celtra Duo I/PC (Dentsply), Lava Ultimate I/PC (3 M Espe),

Fig. 1 – Illustration of the experimental setup with (a) Control tooth. (b) Endodontic access cavity. (c) Situation directly after
root canal filling. (d) Inlay preparation for ceramic/hybrid materials. (e) Inlay dimensions and rounded angles. (f) Cast gold
partial crown preparation with step and bevel.

Please cite this article in press as: Frankenberger R, et al. Stability of endodontically treated teeth with differently invasive restorations: Adhesive
vs. non-adhesive cusp stabilization. Dent Mater (2015), http://dx.doi.org/10.1016/j.dental.2015.08.160
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Fig. 2 – Recordings from the restorative procedures. (a) Partial crown preparation with postendodontic seal (SDR) during
further adhesive pretreatment. (b) e.max CAD inlay after finishing, polishing, and individualization. (c) Celtra Duo partial
crown during adhesive luting in vitro. (d) The inlay from (b) directly after adhesive luting.

and Enamic I/PC (Vita Zahnfabrik, Bad Säckingen, Germany), Feldkirchen, Germany). The mechanical action and the water
all adhesively luted using Syntac/Variolink (Ivoclar Vivadent) temperature within the chewing chambers were checked peri-
after pretreatment of the intaglio surface as recommended odically to ensure a reliable thermo-mechanical loading (TML)
by the manufacturers (HF etching or airborne particle abra- effect. After completion of the thermomechanical fatigue reg-
sion). The chosen indirect conventional procedure was cast imen, specimens were loaded in a universal testing machine
gold I/PC (Degunorm, Degudent, Hanau, Germany), cemented (Zwicki, Zwick, Ulm, Germany) until fracture. Failed specimens
with Ketac Cem (3M Espe, Seefeld, Germany). The experimen- were subjected to photographic documentation (Figs. 3 and 4).
tal set up is displayed in Table 1, compositions of involved For statistical appraisal of recorded results, the
materials are shown in Table 2. CAD/CAM restorations were Kolmogorov–Smirnov test proved values to be consistent
made in a Cerec 3-D device (Sirona, Bensheim, Germany), with a normal distribution curve, so parametric statistical
cast gold was manufactured on stone cast dies according to analyses were carried out (one-way ANOVA and post hoc
the lost-wax technique. Inlay preparations for indirect adhe- Tukey–Kramer test), considering the preparation and restora-
sive restorations were butt-joint, preparations for cast gold tion techniques as variable. The significance level was set as
received a 1 mm bevel. PC preparations for indirect adhesive 5% (SPSS 15.0, SPSS Inc., Chicago, IL, USA).
procedures were cut according to previously published guide-
lines for CAD/CAM restorations (Fig. 1), PC preparations for
cast gold were conventional with step and bevel (Fig. 2).
Thermo-mechanical loading of all specimens – also control 3. Results
teeth – was then performed in an artificial oral environ-
ment (CS4 professional line, SD Mechatronik, Feldkirchen, The results are displayed in Tables 3 and 4. For MOD prepa-
Germany). One specimen was arranged in one simulator rations, statistically higher fracture strengths were recorded
chamber and occluded against a steatite (a multi-component for all groups except IPS Empress CAD (p > 0.05). The results
semi-porous crystalline ceramic material) antagonist (6 mm of partial crowns made of e.max CAD, Celtra Duo, Lava Ulti-
in diameter, Fig. 3a) obliquely hitting cusps of restored teeth mate, and Enamic showed fracture strengths being almost
for 1,200,000 cycles at 100 N at a frequency of 0.5 Hz. The comparable to the control teeth without restoration and com-
specimens were previously subjected to 300,000 thermal parable to each other (p > 0.05). However, cast gold PCs even
cycles between +5 ◦ C and +55 ◦ C (THE 1100, SD Mechatronik, outperformed control teeth (p < 0.05). Amalgam partial crowns

Please cite this article in press as: Frankenberger R, et al. Stability of endodontically treated teeth with differently invasive restorations: Adhesive
vs. non-adhesive cusp stabilization. Dent Mater (2015), http://dx.doi.org/10.1016/j.dental.2015.08.160
DENTAL-2613; No. of Pages 9
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Table 2 – Materials under investigation.


Restorative material Classification Composition (%wt) Manufacturer
Tetric EvoCeram Bulk Fill Nanohybrid resin Dimethacrylate, prepolymer, Ivoclar Vivadent, Schaan,
composite Barium glass, Ytterbium principality of
trifluoride, mixed oxides, Liechtenstein
initiators, and stabilizators
IPS Empress Leucite reinforced glass 57–80% SiO2 , 11–19% Li2 O, 0–13%
ceramic K2 O, 0–11% P2 O5 , 0–8% ZrO2 , 0–8%
ZnO, ZnO, 0–5% Al2 O3 , 0–5% MgO
e.max CAD Lithium disilicate ceramic 57–80% SiO2 , 11–19% Li2 O, 0–13%
K2 O, 0–11% P2 O5 , 0–8% ZrO2 , 0–8%
ZnO, ZnO, 0–5% Al2 O3 , 0–5% MgO
Celtra Duo Zirconia reinforced lithium Lithium silicate with ∼10% ZrO2 Dentsply DeTrey, Konstanz,
silicate ceramic Germany
Lava Ultimate Hybrid A: resin nano 80% Nanoceramic, 20% resin 3M Espe, Seefeld, Germany
ceramic matrix
Enamic Hybrid B: polymer 86% Feldspathic ceramic, 14% Vita Zahnfabrik, Bad
infiltrated ceramic network polymer Säckingen, Germany
Degunorm Cast gold 73.8% Au, 9% Pt, 9.2% Ag, 4.4% Cu, Degudent, Hanau, Germany
2% Zn, 1.5% In, 0.1% Ir
Dispersalloy Amalgam 50% Hg, 25–50% Ag, 5–15% Cu, 1% Dentsply, Konstanz,
Zn Germany
Luting material
Syntac 4-Step etch-and-rinse Etchant: 36% phosphoric acid Ivoclar Vivadent, Schaan,
adhesive Primer: maleic acid, TEGDMA principality of
(triethyleneglycol dimethacrylate), Liechtenstein
water, acetone
Adhesive (2nd primer)
Polyethyleneglycol dimethacrylate,
glutaraldehyde, water
Heliobond: Bis-GMA
(1-methylethylidene)bis[4,1-
phenyleneoxy(2-hydroxy-3,1-
propanediyl)] bismethacrylate),
TEGDMA, UDMA (urethane
di-methacrylate: 1,6-dimethacryl-
ethyl-oxy-carbonylamino-2,4,4-
trimethylhexane)
Variolink II Luting resin composite Base: Bis-GMA, UDMA, TEGDMA,
fillers, ytterbium trifluoride,
stabilizers, pigments
Catalyst: Bis-GMA, UDMA,
TEGDMA, fillers, ytterbium
trifluoride, stabilizers, pigments,
benzoyl peroxide
Ketac Cem Luting glass ionomer Powder: calcium FASG 3M Espe, Seefeld, Germany
cement Liquid: copolymer of acrylic and
maleic acid + water

Fig. 3 – Recordings from the fracture strength evaluation in the universal testing machine. (a) Cast gold partial crown during
loading. (b) Crashing of the roots because for cast gold partial crowns no restoration fracture occurred. (c) Occlusal view after
root fracture in a cast gold partial crown: a distinct plastic deformation of the gold is visible (arrows).

Please cite this article in press as: Frankenberger R, et al. Stability of endodontically treated teeth with differently invasive restorations: Adhesive
vs. non-adhesive cusp stabilization. Dent Mater (2015), http://dx.doi.org/10.1016/j.dental.2015.08.160
DENTAL-2613; No. of Pages 9
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Fig. 4 – Recordings from the fracture strength evaluation in the universal testing machine. (a) Lava Ultimate inlay during
loading exhibiting a cusp fracture. (b) Failure mode in direct resin composite restoration: the resin-composite interface as
well as the cusp tip show distinct fracture lines.

were in the range of natural teeth without cavity or root canal The most pronounced difference was seen among cast gold
treatment (p > 0.05). and amalgam restorations. Whereas PC restorations revealed
For MO restorations, I/PC groups showed less difference in the best outcome throughout the complete investigation, cast
fracture resistance than in MOD restorations, but still with gold inlays and amalgam fillings showed the lowest values
advantages for PCs (p < 0.05). Also here, cast gold PC exhib- for fracture strength for both preparations, however, also here
ited significantly higher fracture strengths than the other the stabilizing effect of the unprepared distal lateral ridge in
restorations and even the control group (p < 0.05). Regarding MO cavities lead to a significantly better outcome compared
restorative materials, it was clearly evident that IPS Empress to MOD inlays made of cast gold or MOD amalgam fillings
showed a generally lower performance than the other mate- (p < 0.05).
rials, irrespectively of the mode of preparation and its Photographic recordings of fracture mode showed cata-
invasiveness. Also fracture modes in these groups exhibited strophic vertical fractures in the vast majority of specimens.
that the limitation in that particular case was not preparation Between the materials under investigation, no single group
design or durability of adhesion but the intrinsic weakness of could achieve less catastrophic fracture patterns than others
the glass ceramic itself. (p > 0.05).

Table 3 – Results (N) for MOD preparations.

Please cite this article in press as: Frankenberger R, et al. Stability of endodontically treated teeth with differently invasive restorations: Adhesive
vs. non-adhesive cusp stabilization. Dent Mater (2015), http://dx.doi.org/10.1016/j.dental.2015.08.160
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Table 4 – Results (N) for MO preparations.

sound tooth hard tissue during preparation of the endodon-


4. Discussion tic access cavity, the root canal, and often additionally of a
post space [19,20,30,52]. This is the reason why – in contrast
Daily all over the world, laboratory scientists try to simu- to restorative aspects of vital teeth – crown preparations are
late clinical conditions in order to predict clinical behavior of often recommended for ETT, and to be honest, clinical find-
dental biomaterials [7,8,17,18,42]. Although it is well-known ings are really encouraging, especially when being compared
that prospective clinical trials remain the ultimate tool for to reports about direct restorations of ETT with inacceptably
the evaluatin of novel restoratives [35], it was described in high annual failure rates [23,37,41,53–56].
the introduction that this is simply not routinely possible. The chosen in vitro setup of the present study already
Moreover, when it comes to more experimental setups, it is proved to give reliable results in several studies with an array
ethically not acceptable to burden patients with possibly inap- of different classes of materials intended for intraoral use,
propriate restorative approaches such as cast gold inlays in the also with a certain correlation to in vivo findings at least for
present study. Especially in questions of more scientific nature the case of marginal quality of etch-and-rinse adhesives vs.
like in the present investigation, preclinical evaluation is the self-etch adhesives [8,43,42]. It simply is closer to intraoral
only favorable way to do so. Whereas in restorations of vial conditions to thoroughly fatigue different kinds of restora-
teeth marginal integrity, wear, biocompatibility and biologi- tives before ultimate loading until fracture is performed in due
cal issues like biodegradation and absence of postoperative course [8]. Compared to previous studies on non-ETT, a higher
hypersensitivities are of primary concern [4,43,44], ultimate fatigue load and a higher number of thermomechanical load
fracture strength was repeatedly investigated in ETT due to cycles was chosen as shown before on zirconia crowns [57].
the fact that 27% of clinical failures are attributed to fractures The chosen materials for restoration of ETT were an array
[45–47]. of traditional vs. innovative biomaterials, and even cast gold
Fatigue loading has considerably gained importance in was included due to the reason that fractures never occur
materials science not only regarding to adhesion but also here. Among the newer indirect materials also hybrid mate-
concerning fracture resistance over time [7,8,48–51]. Intraoral rials such as Enamic and Lava Ultimate were chosen, which
measurements revealed that average masticatory forces are were recently shown to provide good adhesion in vitro [58].
20 MPa, however, also with much higher peak impacts [18,26]. However, it was shown that cast gold inlays and amalgam
It was empirically assumed that in ETT, after access cavity fillings provide the worst stabilizing effect for ETT due to miss-
preparation and root canal treatment, a lower threshold level ing cusp coverage and also missing adhesive stabilization.
for tactile sensitivity would result. Although recent reports On the other hand, cast gold PCs provided the highest frac-
were not able to confirm this, it is still widely accepted that ETT ture strengths all over the study with not a single fracture.
are still more prone to fracture than vital teeth [15,25,26,28]. The forces in the cast gold PC group were so high that finally
Therefore it can be concluded that the reason for increased the roots crashed (Fig. 3b). Another extreme finding was the
fracture risk of ETT is based on the often dramatical loss of behavior of the leucite-reinforced glass ceramic IPS Empress.

Please cite this article in press as: Frankenberger R, et al. Stability of endodontically treated teeth with differently invasive restorations: Adhesive
vs. non-adhesive cusp stabilization. Dent Mater (2015), http://dx.doi.org/10.1016/j.dental.2015.08.160
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Please cite this article in press as: Frankenberger R, et al. Stability of endodontically treated teeth with differently invasive restorations: Adhesive
vs. non-adhesive cusp stabilization. Dent Mater (2015), http://dx.doi.org/10.1016/j.dental.2015.08.160
DENTAL-2613; No. of Pages 9
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Please cite this article in press as: Frankenberger R, et al. Stability of endodontically treated teeth with differently invasive restorations: Adhesive
vs. non-adhesive cusp stabilization. Dent Mater (2015), http://dx.doi.org/10.1016/j.dental.2015.08.160

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