che contnenecepatin eo Ci Blossom Park, 55, Gwanggyo-ro 42beon-gil, Statement March 12, 2021 ‘CJ CheilJedang Corporation hereby certifies that genetically modified strain, E. coli KCCM 80236, was developed from E. coli LS 5218 as a parent strain. This was verified by 16s DNA sequence analysis with the eubacterial universal primers 27F 5'-AGA GTT TGA TCC TGG CTC AG-3" and 1492R 5°CGT TAC CTT GTT ACG ACT T-3", When the analyzed 16s rDNA sequence was aligned with BLAST program, it was 100% identical with E. coli KCCM 80236. ‘Also, the origin of introduced foreign genes were identified by analyzing the sequence of E. coli KCCM 80236. The phaA, phaB and phaC originated from Z. ramigera, sucD and orfZ from C. Kluyveri, and ssaR from A. thaliana were confirmed, Best regards, yn Jisun Lee Cl CheilJedang Corp. Group Manager CTAISAISEASIAL ra 2 OR ‘% This document is electronically signed by CJ CheilJedana Corp. and is leaally effective.Re CHEILJEDANG MATERIAL SAFETY DATA SHEET Lyophilized Microorganism Preparations The information is provided as a service fo our customers and is intended only for their use. ‘This information is based on technical information believed to be reliable and will be revised as new knowledge or experience is gained, Date of issue: October 22 , 2020 Page tof 3 General Information and Company Identification 1.1 Identification ofthe substance: Escherichia cov KCCM 80236 1.2. Use ofthe substance Fermentation use (ete. production of polynydroxyatkanoates (PHA) 1.3. Company Identification = Manufacture's Name CJ CheilJedang Corporation 1.4. Contact for Correspondence ~ Address CCheijedang Center, 390, Dongho-ro, Jung-gu, Seoul 004560, Republic of Korea -Tel +82-2-6740-2457 Fax +#82.31-8000-2117 2. Composition, Information on Ingredionts Each lyophilized, cylinder Shaped FDifeeze drying) vial contains 2 white power made by Freeze drying of the microorganism suspension in 10% skim mik solution, Since this microorganism is classified as biosafety level 1 ‘exposure to theses microorganism in the laboratory rarely causes infection. Effective prevention and treatment is typically avaliable Hazards Information histochemical Not applicable Heath Risk of infection Enviromental Not applicable First-Aid Measures Inhalation : Immediately relocate to afresh air environment and seek medical attention ‘Skin Contact : Wesh with soap and copious amounts of water. mela 1/3Cig® CHEILJEDANG 5 7. 8. 10. Exe Contact: immediately fush eyes with copious amounts of water for at lesst 16 minutes. Assure adequate ‘tushing by separating eyelids with fingers. contac lenses are being wom, remove lenses and continue rinsing Seek medical attention Ingestion: Avoid hand to mouth contact and seek medical attention, Fire-fighting measures Not applicable Accidental release measures Use personel protection, see section 8 Patient Wash with soap and water. Work clothes should be laundered separately. Launder contaminated clothing before re-use, Do not take clothing home EquipmenvEnviconment: Allow aerosols to settle; wearing protecive ciothing, gently cover spill wih paper towel and apply 1% sodium hypochlorite, starting at perimeter and working towards the center; allow sufficient contact ime before clean-up (30 min), Handling and storage ‘The lyophilized microorganism preparation must be stored at appropriate temperature in the orignal sealed container. The |yophitzed preparations contain visble microorganisms that may, under certain cicumstances, produce disease. Proper techniques must be employed to avoid exposure and contact with microorganism growth. The microbiology laboratory must be equipped, and have the facilites to receive, process, maintain, store and dlepose of biohazard material, The ‘microbiology laboratories personnel using these devices must be trained, experienced and demonstrate proficiency in processing, maintaining, storing end disposing of biohazard material Exposure controls/personal protection Respiratory protection Dusk mask or appropriate respirator. Utlize local exhaust ventilation Protective gloves Rubber Other protective equipment Wear appropriate laboratory apparel, protect exposed skin Occupational exposure limits : Not established Physical and chemical propertios White, odorless and dry material Stability and reactivity When stored as directed, the Iyophiized microorganism preparations are stable until the last day of the stated month of the expiration date, The length of storage does not affect the risk of infection. sol) 2/3cee CHEILJEDANG 1, 12, 13, 14. 15. 16. Toxicological information Not applicable Ecological informat Not applicable Disposal considerations ‘Agencies and statutes regulate the disposal of all biohazard materials. Each laboratory must be aware of, and comply ith, the proper disposal regulation of biohazard materials. Transport information ‘Avoid humidity and high temperature. Prevent damage ofthe container. Refer to national and Intemational regulations regarding the shipment and transport of biohazard materials LUN Ciassifcation: UN3373(Biologial Substance) Regulatory information "None especially Other information Contact Person/Point = Laboratory Manager +82-31-8080-2117 ‘Te information above is belleved fo be accurate and represents the best information curently availabe fous. However, ‘we make no warranty of merchantability or any other warranty expressed or implied with respect to such been advieed of the possibilty of such damage information, and we assume no liability resulting from its use, Users should make their ‘own investigations to determine the suitably of the infomation for their particular purposes. in no event shall the company be lable for any claims, losses, or damages of any third party oF for fst profits or any special, increc, incidental, consequential, or exemplary damages howsoever arising, even if the company has been advised of the possibilty of such damage. 12) 8/3