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BIOTECH Q3.Week 3-4 - Science 8
BIOTECH Q3.Week 3-4 - Science 8
GENETIC ENGINEERING:
Manipulation of Genetic
Materials
Prepared by:
AILEEN C. MANALO
Potrero National High School
GUIDE CARD
PRE-
Directions: On your activity notebook,
ASSESSMENT
write the letter of the correct answer.
There are slightly different methods that can be used to put together DNA from
different organisms. This activity focuses on the techniques used to study human
DNA.
By using tools that cut, separate, and then replicate DNA base by base,
scientists can now read the base sequences in DNA from any cell.
Manipulating
A. Cutting DNA
Nucleic acids are chemically different from other macromolecules such as proteins
and carbohydrates. This difference makes DNA relatively easy to extract from cells
and tissues.
DNA molecules from most organisms are much too large to be analyzed, so they must
first be cut into smaller pieces.
Many bacteria produce restriction enzymes that cut DNA molecules into precise
pieces, called restriction fragments that are several hundred bases in length.
Of the hundreds of known restriction enzymes, each cuts DNA at a different sequence
of nucleotides.
For example, the EcoRI (pronounced "eco R one") restriction enzyme recognizes the
base sequence GAATTC.
It cuts each strand between the G and A bases, leaving single-stranded overhangs,
called “sticky ends,” with the sequence AATT.
The sticky ends can bond, or “stick,” to a DNA fragment with the complementary
base sequence.
B. Separating DNA
Once DNA has been cut by restriction enzymes, scientists can use a technique known
as gel electrophoresis to separate and analyze the differently sized fragments.
A mixture of DNA fragments is placed at one end of a porous gel.
When an electric voltage is applied to the gel, DNA molecules—which are negatively
charged—move toward the positive end of the gel.
The smaller the DNA fragment, the faster and farther it moves.
The entire process can be automated and controlled by computers, so that DNA
sequencing machines can read thousands of bases in a matter of seconds.
Card 3
Insulin, a protein that helps regulate the sugar levels in
our blood is normally produced in the pancreas. But in
people with type 1 diabetes there is a problem with
The field of genetic insulin production.
engineering has
revolutionized many People with diabetes therefore have to inject insulin to
scientific fields, from control their blood sugar levels.
fundamental sciences to
medicine and Genetic engineering has been used to produce a type of
engineering. Its insulin, very similar to our own, from yeast and
applications are wide bacteria like E. coli.
ranging, but emphasis is This genetically engineered insulin, ‘Humulin’ was
given to its application in licensed for human use in 1982.
the society. But how genetically engineered insulin is
produced!?
Copy on your notebook and sort the following statements using numbers (in
ascending order) into the correct order to describe how human insulin is
produced.
Guided
Activity 1
ENRICHMENT
CARD
Directions: Copy on your notebook and complete the graphic organizer below by
putting on the letters of the description (What is it?) and its use (How is it useful?).
Genetic Engineering Graphic Organizer
Tool/Process What Is It? How Is It Useful?
Restriction Enzymes 1. 10.
Gel Electrophoresis/
DNA Fingerprinting 2. 11.
Recombinant DNA Technology 3. 12.
Gene Cloning 4. 13.
DNA Sequencing 5. 14.
PCR (Polymerase Chain Reaction)
6. 15.
Transgenic Animals 7. 16.
Transgenic Plants 8. 17.
Transgenic Bacteria 9. 18.
Guided Activity 2
H) Animals that have received DNA from
some other organism
Used to make DNA fingerprints.
Directions: Read the basic information given about cloning. Then study the diagram
below and answer the questions that follow on your notebook.
Cloning is the process of taking genetic information from one living thing and creating
identical copies of it. The copied material is called a clone.
Geneticists have cloned cells, tissues, genes and entire animals.
Although this process may seem futuristic, nature has been doing it for millions of years.
For example, identical twins have almost identical DNA, and asexual reproduction in
some plants and organisms can produce genetically identical offspring. And scientists
make genetic doubles in the lab, though the process is a little different.
There are three different types of cloning, according to the
National Human Genome Research Institute (NHGRI):
Gene cloning, also called DNA cloning, creates copies of genes, or segments of DNA.
Reproductive cloning makes duplicates of whole animals.
Therapeutic cloning creates embryonic stem cells, which are used to create tissues that
can repair or replace damaged tissues.
Directions: Complete the Flow Chart below. Fit in the word(s) from the word
bank in their appropriate places and write your answer on your notebook.
WORD BANK
Personal Identification
No two people are genetically exactly like one another, except for identical twins.
DNA fingerprinting is a method to identify people based on their DNA. DNA
fingerprinting has helped solve crimes and convict criminals. It has also helped prove
that people are innocent of a crime.
Look at the fingerprints below. Then answer the following questions on your
notebook.
_______________________________________________
___________________________________________
4. DNA evidence can be used to solve crimes, convict
criminals and free people who were wrongfully
convicted.
___________________________________________
___________________________________________
1. It involves the manipulation of genetic material (DNA) to achieve the desired goal in a pre-
determined way.
A. BiotechnolgyC. Genetic engineering
B. GeneticsD. Recombinant DNA
2. A process done to manipulate the genome of an organism in order to produce desired traits.
A. gene manipulationC. gene splicing
B. gene pharmingD. gene therapy
3. All the DNA in an organism.
A. cloneB. genomeC. plasmidD. stem cells
4. What is a vector?
A. Any rDNA molecule that has the ability to replicate inside the host.
B. Any DNA molecule that has the ability to replicate outside the host.
C. Any DNA molecule that has the ability to replicate inside the host to which the desired gene has
integrated for cloning.
D. Any rDNA molecule that has the ability to replicate outside the host to which the desired gene
has integrated for cloning.
5. DNA cut with a restriction enzyme produces many smaller fragments, of varying sizes and can
be separated using
A. DNA ligaseC. Polymerase chain reaction (PCR)
B. Gel electrophoresisD. Restriction enzyme
6. Circular DNA molecules that can replicate independently of the main chromosomes of bacteria.
A. cloneB. plasmidC. cellD. gene
7. Animal with foreign DNA in their cells.
A. Genetic engineeringC. Recombinant DNA
B. Human Genome ProjectD. Transgenic animal
8. Which is the most common organism considered for genetic manipulations?A. Bacillus Subtilis
C. E. coli
B. CyanobacteriaD. Saccharomyces cerevisiae
9. The first commercial application of genetic engineering was the use of bacteria to make insulin,
a medicine needed by diabetics. Before the use of genetically-engineered bacteria, insulin had to
be harvested from the pancreases of slaughtered animals.
How has genetic engineering most likely improved the lives of diabetics?
A. It has made the demand for insulin decrease.
B. It has made the incidence of diabetes decrease.
C. It has made insulin cheaper and more readily available.
D. It has made it easier for diabetics to inject themselves with insulin.
10. Genetic engineering is the process of manipulating genes for practical purposes.
How could genetic engineering most likely benefit people with genetic diseases in the future?
A. Genes causing diseases could be repaired through the use of recombinant DNA.
B. Genes causing diseases could be replaced with various types of plant genes.
C. Genes causing diseases could be eradicated from the human genome through the use of
restriction enzymes.
D. Genes causing diseases could be cloned and placed into other human cells.
REFERENCE
CARD
https://www.imedpub.com/scholarly/genetic-engineering--biotechnology-journals-articles-ppts-list.ph
p#:~:text=Genetic%20engineering%2C%20also%20called%20genetic,produce%20improved%20or%20
novel%20organisms.
_(Boundless)/17%3A_Biotechnology_and_Genomics/17.1%3A_Biotechnology/17.1B%3A__Basic_Tech
niques_to_Manipulate_Genetic_Material_(DNA_and_RNA)
https://www.storyboardthat.com/storyboards/meloc1/ge-vs-gm
https://explorebiotech.com/7-important-molecular-tools-genetic-engineering/
https://www.nature.com/scitable/content/ne0000/ne0000/ne0000/ne0000/25886366/diagram_1_2.j
pg
https://www.google.com/search?sa=X&rlz=1C1CHBD_enPH833PH833&source=univ&tbm=isch&q=sto
ryboard+on+the+process+of+genetic+manipulation&ved=2ahUKEwjhjr2U0YPqAhXKDaYKHT7CDnAQsA
R6BAgGEAE&biw=1680&bih=939#imgrc=ZQaMcj
https://study.com/academy/lesson/genetic-manipulation-definition-pros-cons.html
https://www.researchgate.net/publication/233953717_Recent_Advances_in_Genetic_Engineering-A_
Review
ANSWER
CARD
Pre-Assessment Activity Card 2 Assessment 1
1. B 6. D 1. D 4. F 7. C 1. clones
2. A 7. C 2. E 5. B 2. defects
3. C 8. C 3. A 6. G 3. poodles
4. increasing variation
4. A 9. B
5. DNA extraction
5. B 10. B Activity Card 3 6. separating DNA
a) 2, b) 5, c) 1, 7. restriction enzymes
d) 6, e) 4, f) 3 8. hybrids
Let’s Do Some 9. DNA
10. bacteria
Review Guided Activity 1
11. transgenic
1. C 4. E 7. D 1. I 10. Q
2. A 5. F 2. A 11. M
3. B 6. G 3. F 12. K Assessment 2
4. B 13. J 1. 2
5. E 14. L 2. No, the bonding patterns in the
6. C 15.N two samples are different, so the
Activity
7. H 16. O people do not have identical DNA.
Card 1-A
8. D 17. P
1. True
9. G 18. R 3. False. Mitochondrial DNA is
2. nucleotides passed directly from mother to the
3. True child.
4. polymerase Guided Activity 2 4. True
5. color-coded 1. Sheep A 5. Sample 1 and 4 may be identical
2. The nucleus was twins, they have the same bonding
removed from the egg cell pattern.
to make sure that all of the
Activity DNA in the clone is a
Card 1-B single sheep.
1. base 3. The lamb is a clone Assessment Card
2. fragments 4. Sheep B 1. C 5. B 9. C
5. Sheep A and the lamb
3. nucleotides 2. A 6. B 10. C
are genetically identical
4. faster 3. B 7. D
5. bands 4. C 8. C