RANDOX CE

STABILITY AND PREPARATION OF REAGENTS

TOTAL PROTEIN (TP) RI. Biuret Reagent
Biuret Method Dilute the contents of bottle RI with 400 ml of double
MANUAL distilled water, rinsing the bottle thoroughly.
RX MONZZA Stable for one year when sealed and stored at
+2 to +25°C.
INTENDED USE
For the quantitative in vitro determination of Total Protein in serum R2. Blank Reagent
and plasma. This product is suitable for manual use and on the RX
ilute the contents of bottle R2 wich 400 ml of double
Monza analyser.
distilled water, rinsing the bottle thoroughly.
Cat. No
Stable for one year when sealed and stored at
TP 245 RI. Biuret Reagent 2x 100 ml +2 to +25°C
2x S00 m R2. Blank Reagent Ix 100 ml
CAL. Standard Ix 5.5 ml CAL. Standard
Ready for use. Stable up to expiry date when stored at +2
GTIN: 0505527320653 to +25°C.

PRINCIPLE
MATERIALS PROVIDED
Cupric ions, in an alkaline medium, interact with protein peptide
Biuret Reagent
bonds resulting in the formation of a coloured
complex. Blank Reagent
SAMPLE Standard
Serum, heparinized plasma or EDTA plasma.
MATERIALS REQUIRED BUT NOT PROVIDED
REAGENT COMPOSITION Randox Assayed Multisera Level 2 (Cat. No. HN I530) and Level
3 (Cat. No. HE I532)
Contents Concentration of solutions Randox Calibration Serum Level 3 (Cat. No. CAL 2351)
-

RI. Biuret reagent PROCEDURE NOTE

Sodium hydroxide 100 mmol f readings cannot be taken at Hg 546 nm, a standard must be
Na-K-tartrate 16 mmoll used to calculate the total protein concentration in the sample.
Potassium iodide 15 mmol/l The sample blank for clear and colourless sera corresponds to
Cupric sulphate 6 mmol/l approximately 0.2 g total protein/dl and can be ignored.
R2. Blank reagent
Sodium hydroxide 100 mmol/l When icteric (5 mg bilirubin/dl), haemolytic or lipaemic sera are
Na-K-tartrate 16 mmol/l to be assayed, a sample blank made up of
CAL. Standard 0.02 ml serum or plasma and I.0 ml of Blank Reagent R2 must be
Protein See lot specific insert measured. This is measured against water and the absorbance
obtained subtracted from the absorbance of the sample.

SAFETY PRECAUTIONS AND WARNINGS

For in vitro diagnostic use only. Do not pipette by mouth. Exercise
PROCEDURE
Perform a new Gain Calibration with a cuvette containing fresh
the normal precautions required for handling laboratory reagents.
ddH2O. Select the TP program in Run Test screen and carry out
a water blank as instructed.
Solutions RI and R2 contain sodium hydroxide which is caustic. In
the event of accidental contact, flush affected area with large
Pipette into cuvette:
quantities of water and seek immediate medical attention.

CAL contains Sodium Azide. Avoid ingestion or contact with skin or Reagent Blank Standard Sample Sample
mucous membranes. In case of skin contact, flush affected area with
SO SI blank
copious amounts of water. In case of contact with eyes or if ingested,
seek immediate medical attention. Distilled H0 0.0 ml
Standard (CAL) 0.01 nl
Sodium Azide reacts with lead and copper plumbing, to form Serum 0.01 ml 0.01 ml
potentially explosive azides. When disposing of such reagents flush RI 0.5 ml 0.5 m 0.5 m
with large volumes of water to prevent azide build up. Exposed metal R2 0.5ml
surfaces should be cleaned with I0% sodium hydroxide.
Mix, incubate for 30 min at +20 to +25°C before reading as
Health and Safety data sheets are available on request instructed on screen.

The reagents must be used only for the purpose intended

by CALIBRATION FOR RX MONZA
suitably qualified laboratory personnel, under appropriate Recommended on change of reagent lot or as indicated by quality
laboratory conditions. control procedures, using CAL Standard provided in kit or
Randox Calibration Serum Level 3.

Randox Laboratories Ltd, 55 Diamond Road, PAGE I OF 2

Crumlin, County Antrim, BT29 4QY, United Kingdom
T +44(0) 28 9442 2413 F +44 (0) 28 9445 2912 E
applications@randox.com I www.randox.com