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DIAGNOSIS

• PERIPHERAL SMEAR
• SICKLING TEST
• HB ELECTROPHOROSIS
• PCR
SICKLING TEST Principle :
Sodium meta bisulphite reduces the oxygen tension
inducing the typical sickle – shape of red blood cells.
Sample: Fresh blood in any anticoagulant.
Method: mix 1 drop of blood with 1 drop of 2%
sodium meta bisulphate solution on a microscope
slide.

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