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  • Ejercicios Procesos Taller 2

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    Juan Jose Calvo
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    The document contains data from a bacterial growth experiment measuring substrate concentration (S), biomass concentration (X), and other variables over time. It shows the bacteria entering exponential growth phase between 2-8 hours as biomass concentration increases exponentially with time. After 10 hours, the bacteria reach stationary phase where the biomass concentration levels off around 6.2 g/L. Kinetic parameters like maximum growth rate and doubling time are calculated from the experimental data.

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    Ejercicios Procesos taller 2

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    The document contains data from a bacterial growth experiment measuring substrate concentration (S), biomass concentration (X), and other variables over time. It shows the bacteria entering exponential growth phase between 2-8 hours as biomass concentration increases exponentially with time. After 10 hours, the bacteria reach stationary phase where the biomass concentration levels off around 6.2 g/L. Kinetic parameters like maximum growth rate and doubling time are calculated from the experimental data.

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    © All Rights Reserved
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    Download as xlsx, pdf, or txt
    16 views10 pages

    Ejercicios Procesos Taller 2

    Uploaded by

    Juan Jose Calvo
    The document contains data from a bacterial growth experiment measuring substrate concentration (S), biomass concentration (X), and other variables over time. It shows the bacteria entering exponential growth phase between 2-8 hours as biomass concentration increases exponentially with time. After 10 hours, the bacteria reach stationary phase where the biomass concentration levels off around 6.2 g/L. Kinetic parameters like maximum growth rate and doubling time are calculated from the experimental data.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    of 10

    t S(g/L) 1/S X(g/L) 1/X ln(X)

    0 9.23 0.108 0.2 5.000 -1.609


    2 9.21 0.109 0.211 4.739 -1.556
    4 9.07 0.110 0.305 3.279 -1.187
    8 8.03 0.125 0.98 1.020 -0.020
    10 6.8 0.147 1.77 0.565 0.571
    12 4.6 0.217 3.2 0.313 1.163
    14 0.92 1.087 5.6 0.179 1.723
    16 0.077 12.987 6.15 0.163 1.816
    18 0 6.2 0.161 1.825

    ln(Xo) -2.3527 Xo 0.0951 g/L


    µ 0.2919 h-1
    Y(x/s) 0.6496933 Y=(X-Xo)/(So-S) Y=(5,6-0,305)/(9,07-0,92)
    Td 2.3746049 h td=ln2/µ td=ln2/0,2919
    K 0.4211227 h-1 K=1/td K=1/2,3746
    µ;t=10h 0.292368 h-1 µ=ln(X/Xo)/t µ=ln(1,77/0,0951)/10
    Chart Title
    2.000

    1.500 f(x) = 0.291927951642488 x − 2.35265818687983


    R² = 0.999934903175819
    1.000

    0.500

    ln(X)
    0.000
    2 4 6 8 10 12 14 16
    -0.500

    -1.000

    -1.500
    tiempo

    Fase exponencial
    6

    0
    2 4 6 8 10 12 14 16
    14 16
    [S]g/L µ(1/h) 1/[S](L/g) 1/µ (h)
    1 0.083 1.000 12.048
    2 0.143 0.500 6.993
    4 0.221 0.250 4.525
    6 0.273 0.167 3.663
    8 0.31 0.125 3.226
    10 0.332 0.100 3.012
    12 0.351 0.083 2.849
    14 0.367 0.071 2.725
    16 0.366 0.063 2.732
    20 0.368 0.050 2.717

    Ks 4.906 g/L Ks=m*µmax Ks=9,9842*0,491


    µmax 0.491 1/h µmax=1/b µmax=1/2,0486
    ecuacion µ=0,491[S]/4,906+[S]

    m 9.9842
    b 2.035 h
    Lineweaver-Burk
    14.000

    12.000
    f(x) = 9.96455138178246 x + 2.04864826550993
    R² = 0.999427254989891
    10.000

    8.000

    6.000

    4.000

    2.000

    0.000
    0.000 0.200 0.400 0.600 0.800 1.000 1.200
    McA McB McA
    t(h) Ncelulas/ml Ncelulas/ml ln(McA) ln(McB)
    9.00E+06
    0 2.09E+06 2.29E+05 14.55 12.34
    8.00E+06
    1 2.04E+06 2.24E+05 14.53 12.32
    7.00E+06
    2 1.99E+06 2.25E+05 14.50 12.32
    3 2.63E+06 2.19E+05 14.78 12.30 6.00E+06
    4 3.47E+06 2.23E+05 15.06 12.31 5.00E+06
    5 4.68E+06 3.16E+05 15.36 12.66 4.00E+06
    6 6.17E+06 4.47E+05 15.64 13.01 3.00E+06
    7 7.94E+06 6.46E+05 15.89 13.38
    2.00E+06
    8 8.32E+06 9.33E+05 15.93 13.75
    1.00E+06
    9 8.51E+06 1.32E+06 15.96 14.09
    10 8.33E+06 1.29E+06 15.94 14.07 0.00E+00
    0 2 4 6
    11 8.13E+06 1.26E+06 15.91 14.05

    McA McB
    µ 0.2793 0.3574
    Fase exp (t) 2h - 8h (7h) 4h - 9h (6h)
    celulas/ml totale 6.33E+06 8.97E-01
    td 2.482 td=ln(2)/µ 1.939 td=ln(2)/µ
    14.50
    K 0.403 K=1/td 0.516 K=1/td
    14.00 f(x) = 0.357351602
    R² = 0.9998720638
    McA 13.50

    16.50 13.00

    16.00 12.50
    f(x) = 0.252749498228122 x + 14.0450267177985
    R² = 0.98157472990063
    12.00
    15.50
    11.50
    15.00
    11.00
    3 4 5
    14.50

    14.00

    13.50
    1 2 3 4 5 6 7 8 9
    McA McB
    1.40E+06

    1.20E+06

    1.00E+06

    8.00E+05

    6.00E+05

    4.00E+05

    2.00E+05

    0.00E+00
    2 4 6 8 10 12 0 2 4 6 8 10

    McB
    14.50

    14.00 f(x) = 0.357351602615766 x + 10.8783139119582


    R² = 0.999872063825137
    13.50

    13.00

    12.50

    12.00

    11.50

    11.00
    3 4 5 6 7 8 9 10
    B

    6 8 10 12
    Tiempo (h) AbsorvanciaLn Celulas/ml Celulas/ml
    0 0.0022 15.433 5.04E+06
    1 0.0021 15.430 5.03E+06
    2 0.0088 15.569 5.78E+06
    3 0.0192 15.784 7.16E+06
    4 0.0315 16.039 9.24E+06
    5 0.0506 16.434 1.37E+07
    6 0.1108 17.679 4.76E+07
    7 0.1643 18.786 1.44E+08
    8 0.1905 19.328 2.48E+08
    9 0.2732 21.039 1.37E+09
    10 0.2603 20.772 1.05E+09
    11 0.2611 20.789 1.07E+09
    Fase estacionaria = 9-11h
    Densidad fase estacionaria 1.16E+09 Promedio de las tres densidades resaltadas

    Ln N°Celulas/ml = 20,688Abs + 15,387


    F
    21.100
    21.050
    21.000
    f(x) = − 0.12
    20.950 R² = 0.70061
    20.900
    20.850
    20.800
    20.750
    20.700
    20.650
    20.600
    8.5 9 9
    Curva crecimiento
    1.60E+09

    1.40E+09

    1.20E+09

    1.00E+09

    8.00E+08

    6.00E+08

    4.00E+08

    ^ 2.00E+08

    0.00E+00
    0 2 4 6 8 10 12

    nsidades resaltadas

    Fase estacionaria
    00
    50
    00
    f(x) = − 0.125162400000001 x + 22.1181856
    50 R² = 0.700615708543355
    00
    50
    00
    50
    00
    50
    00
    8.5 9 9.5 10 10.5 11 11.5

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