Michaelis-Menten: Velocidad Vs Sustrato

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Michaelis-Menten

Asparato Sin CTP Con CTP


[C] Vs Vc Velocidad vs Sustrato
1 0.45 0.2 Vs Linear (Vs) Vc Linear (Vc)
2 0.8 0.4
7
3 1.7 0.7 f(x) = 0.330128970623358 x + 1.1339144972534
4 2.9 1 6 R²
f(x)==0.847286124105403
0.379197516121328 x − 0.233245760687843
R² = 0.969878205953818
5 3.4 1.4 5
7 4.3 2.4
4
9 5.1 3.7
10 5.3 4.2 3

12 5.6 4.8 2
15 5.8 5.5
1
16 5.8 5.6
17 5.8 5.6 0
0 2 4 6 8 10 12 14 16

Lineweaver-Burk
Asparato Sin CTP Con CTP Velocidad vs Sustrato
1/[C] 1/Vs 1/Vc
1 2.22222222 5 Sin CTP 1/Vs Linear (Sin CTP 1/Vs)
Con CTP 1/Vc Linear (Con CTP 1/Vc)
0.5 1.25 2.5
6
0.33333333 0.58823529 1.42857143
0.25 0.34482759 1 5
f(x) = 5.24140285404686 x − 0.244174358159172
0.2 0.29411765 0.71428571 R² = 0.996656091937166
4
0.14285714 0.23255814 0.41666667
0.11111111 0.19607843 0.27027027 3
0.1 0.18867925 0.23809524
2 f(x) = 2.25841257156398 x − 0.0463620129895316
0.08333333 0.17857143 0.20833333
R² = 0.975299967427024
0.06666667 0.17241379 0.18181818 1
0.0625 0.17241379 0.17857143
0
0.05882353 0.17241379 0.17857143 0 0.2 0.4 0.6 0.8 1

Eadie-Hofstee
Asparato Sin CTP Transformad Con CTP Transformada Velocidad vs Sustrato
[C] Vs Vs/[C] Vc Vc/[C]
Vs/[C] Vc/[C]
1 0.45 0.45 0.2 0.2
7
2 0.8 0.4 0.4 0.2
3 1.7 0.56666667 0.7 0.23333333 6
4 2.9 0.725 1 0.25
5
5 3.4 0.68 1.4 0.28
7 4.3 0.61428571 2.4 0.34285714 4
9 5.1 0.56666667 3.7 0.41111111 3
10 5.3 0.53 4.2 0.42
2
12 5.6 0.46666667 4.8 0.4
15 5.8 0.38666667 5.5 0.36666667 1

0
0.1 0.2 0.3 0.4 0.5 0.
3

1
16 5.8 0.3625 5.6 0.35
0
17 5.8 0.34117647 5.6 0.32941176 0.1 0.2 0.3 0.4 0.5 0.

Hanes-Woolf
Asparato Sin CTP Transformad Con CTP Transformada Velocidad vs Sustrato
[C] Vs [C]/Vs Vc [C]/Vc
[C]/Vs [C]/Vc
1 0.45 2.22222222 0.2 5
6
2 0.8 2.5 0.4 5
3 1.7 1.76470588 0.7 4.28571429 5
4 2.9 1.37931034 1 4
5 3.4 1.47058824 1.4 3.57142857 4
7 4.3 1.62790698 2.4 2.91666667
3
9 5.1 1.76470588 3.7 2.43243243
10 5.3 1.88679245 4.2 2.38095238 2
12 5.6 2.14285714 4.8 2.5
15 5.8 2.5862069 5.5 2.72727273 1
16 5.8 2.75862069 5.6 2.85714286
0
17 5.8 2.93103448 5.6 3.03571429 0 2 4 6 8 10 12
Sustrato
Vc Linear (Vc)

72534
0687843

10 12 14 16 18

Sustrato (1/V0) = (km/Vmax) (1/[C]) + (1/Vmax)

near (Sin CTP 1/Vs)


near (Con CTP 1/Vc)
y = 5.2414x - 0.2442
(1/Vs0) = 5.2414(1/[C]) - 0.2442

59172
y = 2.2584x - 0.0464
(1/Vc0) = 2.2584(1/[C]) - 0.0464

895316

0.8 1 1.2

Velocidad vs Sustrato
Vs/[C] Vc/[C]

3 0.4 0.5 0.6 0.7 0.8


3 0.4 0.5 0.6 0.7 0.8

Velocidad vs Sustrato
[C]/Vs [C]/Vc

6 8 10 12 14 16 18
Actividad para puntos extras
Ejercicio 2
Cte de V
Tiempo (h) Cp (mg/L) Ln(Cp)
2.5
0.25 2.231 0.80244992 2
0.5 1.932 0.65855574 1.5 f(x) = − 0.527903614457831 x + 2.07572289156626
R² = 0.92209044663824
0.75 1.671 0.51342225 1
1 1.45 0.37156356 0.5 f(x) = − 0.577530920856263 x + 0.947086673196738
0 R² = 0.999998443153588
1.5 1.083 0.07973497 0 0.5 1 1.5 2 2.5 3 3.5 4 4.5
-0.5
2 0.812 -0.20825494 -1
2.5 0.608 -0.4975804 -1.5
3 0.456 -0.78526247 -2
4 0.256 -1.36257783
Cp (mg/L) Linear (Cp (mg/L))
Ln(Cp) Linear (Ln(Cp))
Orden cero
89156626 y = -0.5279x + 2.0757
Orden uno
673196738 y = -0.5775x + 0.9471
3 3.5 4 4.5

Linear (Cp (mg/L))


Linear (Ln(Cp))

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