1olslia

Exno: PREPARAIION OE MOHRS 6ALT

AIM
loprepare a pu1e sample of FeTOUS Ammoníurm
Sulphate IMohrs Salt. [Fe s04 (NHaS046Hao]

APPARATUS RENUIRED
_a Beake1S (a5oml, chfnadfsh, unnel. glass zod
unnel stand, Wash boltle, wiye qauze
FerrousSulpbatQ crystais. Amoonium Suiphate
CTyskals,dii: Has04, ethyi alcohol.

THEORY

Mohs salt is prepared by disspluing an equivmolar

mixture Df hydrated Ferrous Suiphate and Amoonium
8ulphate in water coptaining a iitle of suiphuricacid
and then subjecting the resuiting saluion to
cryskalisation uhen gheen ciyatals oi Fexious
Ammoniuum Sulpbate Lfesa4 (NHA),so 6HaD]
Sepaiate out
FesDA 1 H20 (NH4)2 SD4 FesD4:CNH4,SD4 6Hat
Ferrous Sulphoate Ammonium Mahrs salE.
Sulphate
913 29
 PROCEDORE.
A Take a 260 mi beaker and uwash It wlth woater
OBSERVATION and tnen transfer 19 of ferrOUs Sulpbate
and3:5 q of Ammonfum &uipbatec rystals
wefght of the crusEals 7i03- Then add 2-3 mi of dil: Haso4 to prevent thee
obtain 1
hydrolysis of errous Sulphate
* Take anotbe beake and boil 20nml oi water-
FXpected yield
Apr 6 minutes to _expel the dissolued air
COlous Of the CYys tals L r g t GiTeen a add ihe boiling hot watcr to the firat beaker
in small amount and stir it with a qlass vod
Snape O the
crystals mOnoclinic until the salt gets completey discolved
a Ellter the solution to remove the _undissolued
impurlties. and trasfer tharilterate a the china dish.
Heat the solution In the chinadish for some time
.i
until it qets tbe crystallisation point:
Piace the china dish econtaining catiuaked enlLutlon
Dver a beaker sull oR old wate: oD cooling
crystalg DR Mohr[ Salt separatea out
DecantDf the mother liquor quickig and the
wash ibe_cxystals in the cbina slisb_wftb a
Small amaunt ot alcobol ko 1empve the suNphunic acld
Sticking to the Crustals.
Dy ne cTyskals by-placing_them between the
giter paper pad

RESOLT
The puxg sample ofMDbrs Salt is prepared
 4l6l11
EXno:2 PR FPAREATION OE PoIASHAIUM CRySTAIS

OBSERATION

cYJstals 5.3 AIM

wcfght of the
To prepaie apUre sanenple of crystals of patasbalum.
Expectedl yield Ka SO4 Al, CSD)24 HaD
;

Cotor of the cystals: White

APPARATUS REDOIRED-
Shape of the crysals: Optahedral Tuwobeakers (26.0mi)ebinadish, SunneSunnelstand,-
glass od,wasbbottla, bripod ctand, wire quaze
Potassium Sulphate, Aluminlum suiphoate,
dil: Has04

THEORY
PotashAlum fs prepared by dlissahlng an equimolar
oixture of Hydrated Alumlnium Slpbate and Btasstua
Sulphate in_minimum amaunt ot water coniaining a
lîtHle_bfSlphuric aid and nen subjecte the
res.ultiog solutlon to crystaluisatinn when ockahedral
Lryskals af paBashalum separate.out
_KaSD4t ACs04°1RHD+6H0 K S04 Pi,(Go4)3 4HO
14 potash alum

PROCEDURE
Take a 250mi beaker wash 1t wlth uwater apd
Hhen anster 5 otKaSO4CTystals to it 0dd
about20mlOf HaOSHY_D_dissalVe tne
crystals
Take the olh07 ano mi e o k , uwah It u i n uata asd

then tyanu fer log 0 A1s0d, Crystals to 1t hdd acouE

a0 ml o uater and then transfer 1o n i of dute

Hzitig
Lo pevent hydroluyci s of Au S04) Heat for accut
rfout9s L milkiness 6t1 pergists, nen iite1 it
Mfx the Sotuttons fo a Chffra d b ardpiaLa tre
Chfna dfsh. On a wire gua e placed over aeuTner.
9tfr ttbe soutionwih aqlass_aod: Concentrate h e

solutionHU he cngsta.llisaion_pofnt P reathad. Pore te

dFsh
over a beokar contatning Coldunte
* S o o r h e crystals o t -potash aum separake cut:
Decapk

O he mpthe lRquuor Lwash he crystalsaith

asmallquantity of ice-_coldpater
Dry he crystals by PlacYnq thara bl AtGT
paper Pads or by-spreading Over Ene_pomu plake-

RE 8ULT:2
Potaah alum Crystau ar
piepaed
 orginal
line
line ance
solUte

otmula: moed by lire.

Dis
lance
fovm orginal
mDved t solvedt

xn0:15: PAPER CHROMAIO GRA PHY.

calcutatiou

o47

R; 4 AIM
i)Red A
colouiedsobstance present lo the mixture
To sepaiate
ot redand blue
i n k by paperchromatngraphy and iind
Pred < R 4 blue thelr ekardatHon_sactol_

APPARATBS REQUIRED!
alass jo Glass.wd, kilkerpaper Staip(whatmanh Na:1
ilter papgadjan cover,Aneaiuany tube.
REQUIREMENTS
A infs, alcohals aistledwater
mixture_oi redand blue

PROCEDURE
Rectonqulais skip os whatman £iltey paper as kaken_Atith
thehelp ot fine_capillary ube anda drop_ot red.andblue
înk is kept atthe
centreie: 1 crm abnve the bottom of iha_sheel-

Distance travelted Dis tance

The filter papez was kepk in the_conica flask such that
Substance by diiserent trav elled by the botkom touches the alcohal andwater_mixtur
RF Vale
Componenls SOlv ent It isnoticed that the adsoarbent rises withthe blot of mlxture
when left undistur.bed.
Red ink A cro (Mdlnk) ASten the cobapletiDn pt adsDrbentt h eflter
paperi s
?
Ax nemoved_and Rfvalue Is calauated
BUe int RESULL:
Bcm (BluenE
crn
The Tetardation fackoY_of Blue ink greater than
nRdi n k
 ExnD:8 EEFECTIVENESS_OE DIEEERENT coMMON OJLS.
0BSERVATION

for ihe separanion

Time taken
AIM
eottia Name Of
layers
To study the ekectivencss Df dleserent copmmon oils-
OT he o7 wlthout sap uwf soapl
Tube
deteigent detargenu Ccaskor oih otton seed oil, coconut bil kemsangeoil-
mustard oil lo fDrmfng ernulsinn
6oS
Coconut oP SO

Caitoy oP b6s
THEORY2
Depending upo0 the physicalpupeKEtos and cheraical
Kersose 30s tos composition of the ails, comeail form emulsions
Teadiluy coitm uJator ho1oaA man4 ofoers Sormmith
gaE_difeicultu

APPARR IDS
Three stoppered bottlesor baitng ibesmeasaxing-
_cinder, aopsatch Dr SimplgLDatch amLPippetes=

MAIERIAlS REQDLRED:
Castay atl, coton sed oil, caconuboll,kernCene.cl
muskara ofi and soap salukinn ar: scdfum aleate
salutien Clqm dfscolved in aon L Of Asiiled vwateY)

PROCEDURE
Take Sopper bottl6 and aash themnfth ator.
Label_thonn as _A4B, C
Take zml of each o Haa oYlesepanatey in
bofHies-A BC
a Add zomL ok dicliled water o eacb bottle
 Take bottle AStopper ft _and ghake vigomiisly-
for pne mlnute Iben allolw it n skand:-
Note the ime taken for the Ewo layers ta
Separate Out
Similar1y take bottles KandC qndDote the tim
kaken for he s2naration of a layon In each cane
Noun_add daop ot 11 epap SOuion for
Socdtlam oeiate goiLion_to each of the 3 botties cand
Lnd 0ut he_Hme taken_for the tuwo laijox o
Sepasa te kecord the _observahons

RESULT'
I i feclRar_ Prom fbe abOve obs eINatan tha
ofL takes iong aat kime ip qekseparated exam T
emulsion ad ratea land oftakas iae
AminfmLuA inna is akad 3
The decreasinorder of stablikg left ectivenEG is
 1lals
Exno:1 ESTIMAION DE MOHR SALT USINGKMn.04.
volbm Oti
POrette Reading
Votome o KMnO4
SND Mon calt
CmL)
AIM
Tnitlal Flna
soln Cm)
TO deterine the Molaaity ana strenqth of KM004
Y ain fermuu amnonluxn sulphate. IeAs
90mL : G
20
APPARATUS REQUIRED
20m 0
Lb2unette, PBLsette Stand. Beaken pippalfa
conicalflask and funne
elculation
THEORY
VIM VM
kMnD4 oxidises Fe tn 84 as he follouwIn4
20mL
Jonic 8quaion.
Vblume KMn04 CV)
Bfe 2 Fe 5e-=
NO 0 moles ot Mn Ch =

Mn04 RH Be Mn 4 4HD
VolUme O mohYS Lalt soln CV2) 20mL MnD4+ 8H'45Fe Mn2 4 Ho +4Fe B
NO OHmoles of mohrs Lalt (na) =S
PROCEDURE
O1aTiy o MDhrs salt 2) O oS
=

MOhr salt soluHon is pippeled but In a

20 x M

20 X ODS clean concal flask.

men add 2oDL of dPL: H2SD4. soluHeu
M O O1t
KMn04 is taken In the Buaette:
Stnength Moarity x Molay ma te of Tme content Di he Coni Cal tlaAk Js titrated againatE
KMnD
KMnDA
ale pPok ploua tsobseryaol.
Titraion iS1epeoted unHI fha concacdant da
RESULT
AIne wuolaatyot KMnD4 Is oOIM
A Theskrength of KMnoq_ s 611lmi
 n184t4k
10
Exho:4 ESTIMTION OF KMno4 vsIN
OXALIG ACID. (

Vo\UmL al
BUYette Readlng
Yolurne KMnO4
Cml ALM
Oxoic na
SNO Tntia
aio
Cm loprepaie oaitc actd colth h e heip of mQtarl y
and stnengBh-oKMno4 sDluiu
20

APPARATUS REQuIRE D.
- C o n t a a l e l a s k f u D n e l A B O . m i B e a k e Votunnctrie

flask, wire qauzeBUnette andpippea

Ilonior
THEORY
DYa r a l d 6
'eCy'o o
Oxatcacid Is_Oxidsad to tOa by tme oxidtsing
MOOI t u v o1 w t * O l U v
vit Ovotc ocld
agonE KMnD4aA follo
a KMn04+3Ha S4 5Ha Ca04 Ka sog t8OtaMaDs1 1OCA
Kn0a nEun04 CKMna4+8Ht* Be Mn +Ho)
Chatcattd ovaticacid CCa04
Mno4+OH +9C204 Mn 8HOt1g coa
20 oos KMn04is2elf=_tnadtcator aPzearance o f
Pala pin acoo 1s end poin6

Sstrengn o kMnO4 M tMo1aviy PROLEDU RE

(9 XO 02 aDmL of 0caic actd is taken in a clean conical flask

a0 mlof Suiphuric actoal ts acdd o t: and the content

ot the f lask is heated at B0°c
Pala pink colou is Oeved: Repaat tha pnacedure
until tha concorkant
reaaig is Dblatned
alalh
Ex no: PREPARATION OE SIARCH SOLUTIDN

AIM:
To prepane the_colloldal solution.(soL) 05 starch

APPARATUS REQUIRED
=

Beakers,Ca5Dml)_and (50mi),qlass rod funnel

Silterpaper, pestle Tripod Standwireqauxe and burner

MATERIALs REGUIRED:
Soluable starch Clq) and distlled watey-

ROCEDURE
1q of skarch is taken andioml o _distilled water 1s
added

qrind the
storchomake a thin paste_and transtar the
PAsta_to a 6oml Beaker.

Jake 10om of_diskilled water n a 260 mL baker _and

heat the benker so that tha water wil stait botling
Pour tha posta slowly biy sHrring into lho boiling_
water in the
beaker
_Continae otiing for about 10min and hen allauo the
beakey to cool
FlUter the coDtent o£ the beakar threugh afilker poaper
which is tixed in a funnel and Label the iiltrate as
Starch sal

RESUL:-

yogmite starch soiution is prepar.ed

51H1 8)
Exno:4 REPARATION OF COLL01DAL soLUTIDN OF EG ALBUMIN.

AIM:
o
prepare the Lolloidal solukonCor SoL) Of egg_albLumin:

APPARATUS REQUIRED
Bea ker
L25oml)andé6onmL), egqdistlled snater,
glass rod, Hanne, wire gauzepestle and burner

THEORY:
E49 albumPa_uuhfch Pa chtained fom eggsorms igaphillie
Sol wi olc water The sol is quite stable and s nak
alsected by Ehe_presence of impir ikies.

MAIERALS REQUIRED
Fgg disklled water

PROCE DURE
Break hc outer
she1 ok the egg by stricking ik with glass Dd
and coiLect I F colouYlCss lquid along with yelloo uolk:
Iyansfer the (olourless liauid into ottner beaker. mats
colourless liquid l s named as e44 albumin

Prepare ioDmL o solukion_ Of Nacl In a J6a ml

beaker
To this sojutian egq aloumin is addad în semall partlon
pIth constantsHTYing Tbis _pncesS Should take place Within
1p n0 min
* FlEoY he Lontent OE
f h e beak Qy fhrOugh a HltRr

paperwhich isixed tn_thaPLonel_and

Collect the filt erateand labelthe fil terate a2s
egg albumin _sol

RESDL
Eg4 albumin ispYApared
no:6| IEST EOR PRDIEINS

AIM
To studs someY gimpleutest fo pratelnsB

APPARATUS REQUIRED
egg albumin, dispersion. ,qelatln dlsperslon, nliahydab
Teagent milloas 1engenA

PRDCEDURE:

Experiment Obsexyation. Interence

1:Biuret test
To khe dispersion. of Bluish
V lolek Tndicates th2
the substance_ ko be tested,| Lolar is pRsence o£
add Aml pf NaDH observed pmteins.
Solution: and tnen cid
4-3 dmps of 1Y. af lusaQ4
S0lutio12

2 Xantho proteie teste

Take abDIAtRml af Yelloun ppt Indica kes ha
gg alblimin dispeTsion is oblained _presernce af
in a test ibe_and then PDteins
ad a fe drops of
Con HNDz and it is being.
heated
3. Ninbudrin kost:
take 2ml oE A Intens blue Contirms the
albumio dispersion în a2 PPE Isobkalned: presence pk-
test eilbe ad then pmleins
add a-4 dmps of
ninhydrin solution and
bail the test tuoe.

4: MillonsTest
Take -Amlo£ eaa wlnltePpt Confirms the
albumin dispersion which chanqs presence o£
and han addAdops ko brick red Pokelns

Df mlilon[ 12aqent- on baling..

REOLT
Ihe qiven_Sample is icdenifted as pmteins
 Ex nD6 EFFECT OF CONCENTRATIDN ON THE
RATE Oe THE REACTION

AIM:
To _studs _the eiect of corcentraHon_on the rate oi
he reacton betwen sodlumThio Sulphateand Hsdmehlarie2
acid.

APPARTUS RADUIRED
Skopuatch.2 Bhrettes_and s conical£lask LiaDm)

MATERIA3 REQUIRED
01M_ot 6odium Tnia SuLphal and IMpf HCL-

PROCEDORE
JWash fhe conical flask with water_and label khem ds

*wih the help ot the burette add io20, 30,40,and BD.ml

Of Iodium thlosulplaate Solutlon to the Hask L2,3,45
Tespactively
Alo add 4oao 1RD and io ml at distiled water to the
iask 23,4 respecively so thah the volume et tha
Solunon n_each task_is 5om
Take iQml ni HCL in a Lest tube with ihe help ot the
VolUme o thio Tn Ynl
ounette
AddLomL o£ HaL in a tesE RILe kD the cantcal flask.
no: nfS Slask cantalnlng Loml ef OlM_o6
30aluna Tuin Suiphate and som o dishlled water
and 8kark_the 8topwatch when half O{ ne
 2D

HCI SOution has been added Shake tne content

Reactlan:
_Ot the oncal task_and placa lt on the tile
2NOC SO Ho Gan on observing fiwm top o_down wards 2o the-
Hask and stop he stop iwatch when the crossroauk.
s+
Va SO DHC
Junction betpmes invigible and then note down the-
Observahon. bime
Repeat he experiment by addinga loml of 1M Df
HCL to the fiask R, 3, 4 and 5 ad necod the
volu me of vo lUme o lume Total Time
Hme taken iu each cOse r e the cos to become
o volume Taken
NOa Sa D HC
H0
-

Juat invisible
Cml) cm L
cm

6 320 O D03125
50
60 43 O 002292
A0
20
60 600 O' 001666
30
30
60 1120 O o00 &92
20
40
O 9980 O o0o1

RESOLT
pmportional-
Fmm the graph it s clear that T is direcdly
AediumThingulpnate Goluton
kg the L0ncontration of
but T ls directly pmportional in the _concentration D£
SodlumThioSulphcte which Is the direct meaaure ot Hhe
Neqction
 lela

Fxo: KATE OE THE REACTION WTH RESPECTTO TEMDERATURaE

AIME
tae
To
Study the eheato f_changent e r m peratuie with
rate of the _reacion botween Sodium Thia SuLphate_and
Hyduo cnLoric acid

APPRRATUSREQUIRED:
Conicalftask A6omi m2asuring cyuinder CO and smi)-
Volume o
TempeTaure t fme
Stup 1Natch ThennametesTaipodstandwlfe qauze-
olume of TA ken
HC Tafsod and BUrner
S NO Noa Sz03

28 MATERIALS RESUIRED:
0'c disHlled
15 OLM Sodlum Thinsulphae O YM OA HC
10'c
0 12 Water and Con HAIO3
20'c
30c THEORY
in_a-
The rate of the chemical reactton depends
10 4o'e qreat exkent _upon temperature. and The rate of the
50
increasos with increcke in_kemperature-
rat reacion
EDergy at
increase fa kampeyaura IncreaaOA ttha kinetic
mole ceules baviog
Hhe molecules There fore The racion at
energy wnich then
enargy greater than it threshhDId
nD: D&
increaseA and restlk in increase in he
TE ha baen observed
KH ective coIlisiDn per second
theasessDY every lacrise fn
that ln moskof
at tne 19ackion almast becnmas
Femperatura ne rate
olouble
SDdlum ThiDSuIphate
The Yake ot he reacon lbekueen
Pafsed Tempexattna=
RESOLT
The rate_pf r2acHoa between the Sodfumthin
SIphate and HC fnereases wfto Pncrease
fa
Temperatare
18lsl
Exno'9 ANALYSIS OF THE 61IVEN ORGANIC coMPOUND. 2-

AIM

To test the the functi onal qmoup prasent m fhe

Dranccompound

PROCEDURE

EXperiment Obser vaton Tnference.

xTOllen[ Test :
a m l ofsiverNitrate SPLver N{Erate Presence DE

is taken fn a tesE Obbaioes aldebyde f

ube and then tew the fonerall Contironed.

alros of NH4 OH 1S O tne kosttuube

added fn exeess.
along _nfth the given
organi compound.anD
the inhale mixture is
beaked înawater balh.
Feitings Test
imi af Fenlingssoin
A_and B S OakenIna Red ppt ts Presence of
kcs tube alpog o Pth ie tormed aldehyde is
gien ELQaoke.conpoind Confirmed
and iho mfrkure Fi
eatodfo a wa ter bo
o r -LO rmin
RESULT:

me afven orqanfc coopound fs denHtied aA aldehgde

5lal14
Ex no: 10 NALNSIS OF THE GIVEN ORGANIC COMPoUND 3)
AIM
No test the
functionalqDup present ln me O1ganic compoona.

PROCEDURE

EXPERIMENT O3SERVATION INFERENCE

i)sodium Nitmpurrieide test
To thegiven oxqanic Appeaaance o Indicakes the
compeund 8odium
Nitopuxlsside Red coliDred presence of
Is added and then teu sOluHiDu KeiDne.qoiup
dhops oSodtum Hydmxide
So tuhou is addad

i)Tocdoformtest-
TO he glven orqanic YellotoPpt: is- Pres.ence of
Compauund exeess Ot indine obtained: Ketonegroup iss
SolLLtion is added ConfLrned:

RESULT

Ihe qiven_orqanic-comnaupd is_identified as

Kekon2
1414
x*no:1 ANALYSIs OF THE 6IVEN ORGnNIC coMPOUND(LL)

AIM
o testFhe functional Jroup present ln fhe-
Onganic compound.

PROCE DURE

ExPERIMENT baSERVATION RESULTL

i Litnus test
he qiven oraanic compoupd Coloua chang2 May be.
Is dmpped on. he ALmuA frDm Aue toD acidlic
Papea Rd

i Ferric Chloride Test: VioleE coloua Pnenol-

To Hhe 4len is obtained iS pzaesent:-
osgAniC
-
Compound Feraicehloridle
solutiDia is added:

i) Bpermanítest Red colouA PhenoD

To the given _orqaniccompond ls obtatned may be
Liber mank so1u Eion and jaresent
conHsD4 is added-

Av Phthalein.dye
test:
Th theqiven orqanic
Compaund Ps taken fn
a_beaker and 2
 Hhen fou daops o1 Radcolo Paesenca oi.
Soafum Hyatoxide sOblained at. Phenol
Soluhon Is odded fist and then is cantirmed
HhecoloL Ps-

chargaa
jxeen

RESULL
The 4Íven oZanic compouDd is identified
a phenol
Erno1 ANALY319 OF THE GlVEN ORGIANIL tOMPOUND-

AIM
To tast tne functtonal grOup prasent inE
orqantccompoupd.

PROCEDURE:_

E XPERIMENT OBSERVATION RES OLT

i LUtmus tesk
blue colo- May be
Thegiven organfc
ehange
n actdic.
COmpound fa dhoped ew
the eitrpA papea e d

To tha qiven organiG- Brisk etferyErCandz= Organic-

Lompound KlaHLo% is- is obsexued acids nmay be

pesent.
added

ii To the 4iven onganlt- EIuly Oaganle-

Compound altohoi ia Smell ts acta is
Obtained cen fined.
added and hen feuw_
dhops ot onH2S04 Ig-
Cddd

RESOLT
as
The qfven orZants comPOUnd_is_Jaten tlfied
OqanBe
acid..