Calculation of chemicals in isolation of DNA from vegetables

Calculations for Tris HCL

Data
Tris HCL= 1M
Molar mass of Tris HCL=121.14g
M1=1
V1=1000ml
V2=300
M2=?
Calculations:
M1v1=m2v2
Putting given data in the formula

1 x 1000= m2 x 300

1 x1000 = m2
300
3.34 = m2

The mass of Tris HCL will be= moles x molar mass

3.34m x 121.14g/m

= 404.60 g
 Calculations for EDTA

Data

0.80 moles EDTA

Molar mass of EDTA=181.6g/mol

M1=0.80

V1=100ml

V2=50ml

M2=

M1V1= M2V2

10 x100ml =M2 x50ml

0.80 x100 =M2

50

0.80 x 2= M2

M2= 1.6

Required mass of EDTA= moles x molar mass

= 1.6x 181.6

= 290.50g
Calculations for NACL

Moles of NACL=10 moles

Molar mass of NACL=58.44g/mol

M1=10 moles

V1=1000ml

M2=?

V2=200ml

Putting above data in the formula below

m1v1=m2v2

m2= 10 x1000
200
M2=50
The required Mass of NACL will be
Moles x molar mass
= 50 x 58.44
=2922g