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Physioex Lab Report: Pre-Lab Quiz Results
Physioex Lab Report: Pre-Lab Quiz Results
You correctly answered: Enzymes are specific about the substrates they can act upon.
4 Proteins and peptides are formed by joining amino acids with a special type of covalent bond called a peptide
bond. Which of the following enzymes do you think would be specific for a peptide bond?
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3/8/2020 PhysioEx Exercise 8 Activity 2
Experiment Results
Predict Question
1 Predict Question: Do you think test tube 3 (amylase, cellulose, pH 7.0 buffer) will show a positive Benedict's test?
You correctly answered: see what a positive Benedict's test should look like.
Experiment Data
Tube No. Reagent 1 Reagent 2 Reagent 3 Time (min) Temp (°C) IKI Benedict's
1 Amylase Starch pH 7.0 Buffer 60 37 - ++
2 Amylase Glucose pH 7.0 Buffer 60 37 - ++
3 Amylase Cellulose pH 7.0 Buffer 60 37 - -
Deionized
4 Cellulose pH 7.0 Buffer 60 37 - -
Water
5 Peptidase Starch pH 7.0 Buffer 60 37 + -
6 Bacteria Cellulose pH 7.0 Buffer 60 37 - ++
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3/8/2020 PhysioEx Exercise 8 Activity 2
3 Why was the cellulose in tube 6 (bacteria, cellulose, pH 7.0 buffer) hydrolyzed to glucose?
You correctly answered: The bacterial suspension contained the enzyme cellulase, which digested the cellulose.
4 Which tube allows you to conclude that the buffer was not contaminated with amylase?
2 Describe the result in tube 3 (amylase, cellulose, pH 7.0 buffer). How well did the results compare with your
prediction?
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