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The Feasibility of Fish Amino As Probiotic To Enhance The Growth of Lettuce Lactuca Sativa
The Feasibility of Fish Amino As Probiotic To Enhance The Growth of Lettuce Lactuca Sativa
(Lactuca sativa)
Marlon Anos
Jizabelle V. Baque
May 2022
Chapter I
INTRODUCTION
Lettuce is one of the most widely consumed vegetables worldwide (Kim et al. 2016).
option for those who want to lose weight, as lettuce is low in calories, fat and sodium, as well
as a good source of iron, vitamin C, folate, and fiber (Kim et al. 2016).
Many varieties exist with their own unique flavors and nutritional properties, with
romaine and iceberg as the most common types. Between iceberg and romaine lettuce, the
crisphead (iceberg) lettuce were lower in all nutrients analyzed compared to romaine lettuce
(Kim et al. 2016). As such, romaine lettuce is the most viable option considering its
considered impractical due to its tropical climate (Gonzaga et al. 2018), where the delicate
Probiotics
There have been several ways humanity has profited from the usage of probiotics.
From providing countless health benefits to humans to enhancing the growth of plants, such
acts were made possible with the use of probiotics. The role of probiotics towards the field of
agriculture, particularly plant growth and quality, is developing, along with the discovery of its
effects on plants.
Probiotics adhere to the root and feed the crop daily with no leaching. By building up
natural resistance in plants, usage of fertilizer and pesticides can be reduced or eliminated
(Goh and Bulawan 2018). Moreover, the production of phytohormones, antibiotics and lytic
enzymes, fixation of atmospheric nitrogen, solubilization of soil mineral nutrients, and
induction of systemic resistance in host plants, are beneficial effects probiotics provide to
Philippines, due to the pandemic restricting one’s entertainment, plantita and plantito’s, a
term for those who began to develop a hobby of growing plants, have appeared and spread
amongst the community. As such, common materials that are accessible in one’s home have
been used as an improvisation, a replacement for store-bought fertilizer. Fish amino is one
of those materials which have the potential to be a probiotic, as not only has it been involved
in multiple researchers regarding its capabilities as a probiotic, fish, specifically fish waste,
where fish amino comes from, is easily attained within the town of Llorente.
One of the organic preparations used to improve crop plant development is fish
amino acid (FAA) (Priyanka et al. 2019). FAA is used as a nitrogen source to improve growth
and size, during the early or vegetative stage of development (Weinert et al. 2014). Foliar
application or soil drenching of fish amino acids may also boost uptake and prevent runoff or
leaching, giving the plant enough nitrogen for chlorophyll output to sustain plant health
the effects of fish amino acid (FAA) application on growth and development of okra
(Abelmoschus esculentus) with different sampling times. The number of weeks and the
volume of FAA as a single factor were found to have a substantial impact on shoot length
(Johari et al 2020).
The main problem of this study is to test the feasibility of Fish Amino as a probiotic to
enhance the growth of Lettuce (Lactuca sativa). Specifically, this research aims to answer
sativa)?
2. What amount of Fish Amino Acid is the most effective in enhancing the growth?
i. 1 tablespoon
ii. 3 tablespoons
iii. 5 tablespoons
3. Are there effects that can be observed between the lettuce with the FAA probiotic and the
i. Height
ii. Width
1. Fish Amino Acid as a probiotic can enhance the growth of Lettuce (Lactuca sativa).
3. The FAA probiotic has better effects on the growth of the lettuce than the lettuce that is
1. Determine if applying FAA on lettuce can enhance the growth of the lettuce.
2. Determine what amount in tablespoon is the most effective in enhancing the growth of
lettuce.
3. Compare the lettuce applied with FAA and the lettuce grown normally in terms of size
researchers will use are affordable and easily accessible. By determining the effect of FAA
expected that people would have a greater production and better quality of lettuce. Fish
with materials that do not contain hazardous substances. The researchers will create the
FAA probiotic, to prove that it has a significant effect on the growth of lettuce. Those who
wish to have a quicker production and better quality of lettuce will largely benefit from this
study.
Theoretical Framework
Fish Waste
Fish Waste
Fish Amino Acid
(FAA) is a liquid made
from fish waste. FAA is
a great fertilizer for soil
and plant leaves
because it is rich in
nitrogen which helps
improve the growth of
the crops during their
vegetationFish
periodwaste and
Fish Amino
(Hubilla 2020)Molasses
Acid as together, is
Probiotic toexpected to
Enhance thecreate an
effective
Growth of probiotic to
Lettuce enhance the
(Lactuca growth of
sativa) Lettuce (Lactuca
Molasses
According to asativa)
master
gardener, molasses is high
in calcium, magnesium, iron,
and Fish Waste It also
potassium.
contains sulfur and a host of
micronutrients. Using
molasses as a fertilizer
provides plants with a quick
source of energy and
encourages the growth of
beneficial microorganisms
(Patterson 2021).
Chapter II
METHODOLOGY
(Lactuca sativa)
Sub-Problems:
1. How feasible is Fish Amino Acid (FAA) in enhancing the growth of lettuce (Lactuca
sativa)?
2. What amount of Fish Amino Acid is the most effective in enhancing the growth?
i. 1 tablespoon
ii. 3 tablespoons
iii. 5 tablespoons
3. Are there effects that can be observed between the Lettuce applied with the treatment
i. Height
ii. Width
Hypotheses:
1. Fish Amino Acid as a probiotic can enhance the growth of Lettuce (Lactuca sativa).
3. The FAA probiotic has better effects on the growth of the lettuce than the lettuce that is
1. To determine if applying FAA on lettuce can enhance the growth of the lettuce.
2. To determine what amount in tablespoon is the most effective in enhancing the growth of
lettuce.
3. To compare the lettuce applied with FAA and the lettuce grown normally in terms of size
Variables
The first thing the researchers will do is plant the seeds on planting boxes for the
seeds to grow into seedlings. The second thing that the researcher’s will do is prepare the
Fish Amino Acid (FAA). In making the FAA probiotic, the researchers will need fish waste
and molasses. The researchers will go to the market to gather the fish waste and then they
will wash it to drain the blood. Then, they will measure the fish waste’s weight and mix it with
equal parts of molasses. After preparing the mixture, they will put it in a container with a tight
lid to be fermented for 28 days. By the time the fermentation of the FAA probiotic is finished,
they will prepare the different amounts of FAA in separate containers to be mixed with 1.5
liters of water. But for the researchers to conserve time, they will prepare the plots that will
seedlings and divide it into 4, making each group consist of 10 plants. 1 group will be the
control; 1 group will have the 1 tbsp FAA and 1.5 L water mixture; 1 group will have 3 tbsp
and 1.5 L mixture; and 1 group will have the 5 tbsp and 1.5 L water mixture. The lettuce
seedlings will be then planted in the plots that were prepared with each group being 1 foot
away from another. Then, the researchers will apply 4 full sprays of FAA probiotic once a
day every after 2 days for 4 weeks. In recording the results of the experiment, the
researchers will make an observation on the plants weekly by taking pictures and measuring
the height and width of the lettuce. This will be done in order for the researchers to make an
analysis on the effects of the FAA probiotic on the lettuce in terms of size and rate of growth.
In analyzing the results, the researchers will take all of the measurements of each plant on
each group then take the average growth of the lettuce on each group and accept it as the
result.
This process will be done 3 times to ensure the effectiveness of the FAA probiotic in
The expected outcome of this study is that Fish Amino Acid as a probiotic will
enhance the growth of lettuce than the lettuce grown normally with only water. The 3
tablespoons of FAA combined with 1.5 liters of water is expected to be the most effective
growth enhancer compared to the other amount of FAA combined with water. It is also
anticipated that the size and rate of the lettuce will increase.
The people who will mostly benefit from this study are the people who want to gr\ow
lettuce quickly. And it will also be beneficial to those people who demands an organic growth
enhancer.
The FAA probiotic is expected to improve the size and rate of growth of the lettuce.
And, if these results are met, the production rate will be higher. Also, the FAA probiotic is
organic which makes it not harmful to the people and the environment. It is cheap and easy
to make; therefore, people won’t be having problems with the possible complexity of the
process. In addition, the materials needed to make the FAA probiotic is cheap and easily
accessible. And with these, people don’t have to worry about the money they’re going to
A. Pre-Implementation Stage
1) Gathering the 1 Day Researchers Completely
materials gathered the
needed to materials
create the
Feasibility FAA probiotic
of Fish 2) Creating the 1 Day Researchers Finished
FAA probiotic creating the
Amino as
FAA probiotic
Probiotic to
3) Fermentation 28 Days Researchers Finished
Enhance the of the fermenting
probiotic the probiotic
Growth of
B. Implementation Stage
Lettuce
1) Start the first Four Researchers Completed
(Lactuca trial weeks the first trial
sativa)