SMK (P) SRI AMAN

BIOSCORE MODULE

Paper 3
Biology 2021
(SET A)
NAME : ………………………………………………………………………

FORM : 5 SAINS …………………

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SET 1 : Activity 3.1, Page 50, Form 4
TITLE : Studying the Movement of Substances Across a Selectively Permeable Membrane
OBJECTIVE : To Study the Effect of Different Concentration of Sucrose Solution On The Mass
Of Visking Tubing.

SAMPLE QUESTION:

1 An experiment was carried out to study the effect of different concentration of sucrose solution on
the mass of visking tubing.
Satu eksperimen telah dijalankan untuk mengkaji kesan kepekatan larutan sukrosa yang berbeza ke
atas jisim tiub visking.

Step 1/ Langkah 1:
A visking tubing is cut at 15 sm long. Then the visking tubing is immersed in water for five minutes
to soften it. One end of the visking tubing is tied by using a thread.
Satu tiub visking dipotong sepanjang 15 cm panjang. Kemudian tiub visking itu direndam ke dalam
air selama lima minit untuk melembutkannya. Satu hujung tiub visking diikat dengan benang.

Step 2/ Langkah 2:
The visking tubing is filled with 10 cm3 of 10% concentration of sucrose solution. Then the other end
of visking tubing is tied by using another thread.
Tiub visking diisi dengan 10 cm3 larutan sukrosa dengan kepekatan 10%. Kemudian tiub visking
diikat dengan benang yang lain di hujung satu lagi.

Step 3/ Langkah 3:
The outer surface of visking tubing is rinsed with water. The visking tubing is immersed in a beaker
contains 200 ml of distilled water for 30 minutes as shown in Diagram 1.1
Bahagian luar permukaan tiub visking dibilas dengan air. Tiub visking direndamkan ke dalam bika
yang mengandungi 200 ml air suling seperti ditunjukkan dalam Rajah 1.1

Step 4/ Langkah 4:
After 30 minutes, the visking tubing is taken out and dried by using a tissue. Then it is weight by
using electronic balance. The result was recorded in a table.
Selepas 30 minit, tiub visking dikeluarkan dan dikeringkan dengan menggunakan tisu. Kemudian
ditimbang dengan menggunakan penimbang elektronik. Data direkodkan ke dalam jadual.

Step 5/ Langkah 5:
The experiment is repeated by using different concentrations of sucrose solution which are 20%,
30% dan 40%.
Eksperimen diulang dengan menggunakan larutan sukrosa yang berbeza kepekatan iaitu 20%, 30%
dan 40%.

Diagram 1.1/ Rajah 1.1

2
Table 1.1 shows the result of this experiment.
Jadual 1.1 menunjukkan keputusan eksperimen ini

Concentratio Mass of visking tubing (g) Difference in

n of sucrose Jisim tiub visking (g) mass of visking
solution (%) tubing after 30
Kepekatan minutes
larutan Initial mass Final mass (g)
sukrosa Jisim awal Jisim akhir Perbezaan jisim
(%) tiub visking
selepas 30
minit
(g)

10
…………………….
.

20
…………………….
.

30
…………………….
.

40
…………………….
.

Table 1.1/ Jadual 1.1

(a) Record the differences in mass of the visking tubing in the space provided in Table 1.1
Rekodkan perbezaan jisim tiub visking dalam ruangan yang disediakan dalam Jadual 1.1.

[4 marks/ 4 markah]

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(b) Construct a table and record all the data collected in this experiment.
Bina jadual dan rekod semua data yang diperoleh daripada eksperimen ini.

Your table should have the following titles:

Jadual anda hendaklah mempunyai tajuk-tajuk berikut:

• Concentration of sucrose solution

Kepekatan larutan sukrosa
• Initial mass of visking tubing
Jisim awal tiub visking
• Final mass of visking tubing
Jisim akhir tiub visking
• Difference in mass of visking tubing
Perbezaan jisim tiub visking
• Rate of osmosis unit (gmin-1)
Kadar osmosis

Rate of osmosis
Final mass – Initial mass
Time taken

[3 marks/ 3 markah]

(c) State one precaution that can be observed in Diagram 1.1

Nyatakan satu Langkah berjaga-jaga yang boleh diperhatikan pada Rajah 1.1

…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
[1 mark/ 1 markah]
(d) State the hypothesis for this experiment
Nyatakan hipotesis bagi eksperimen ini.

…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
[2 marks/ 2 markah]

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 (e) State the variables for this experiment and method to handle the variable.
Nyatakan pemboleh ubah bagi experiment ini dan cara menggendalikan pemboleh ubah.

Variables Method to handle the variable

Pemboleh ubah Cara mengendalikan pemboleh ubah
Manipulated variable:
Pemboleh ubah dimanipulasikan: ……………………………………………………………………………..
……………………………………… ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
Responding variable:
Pemboleh ubah bergerak balas: ……………………………………………………………………………..
……………………………………… ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
Constant variable:
Pemboleh ubah dimalarkan: ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
……………………………………………………………………………..
………………………………………

[3 marks/ 3 markah]

(f) Based on the results of this experiment, state the operational definition for osmosis.
Berdasarkan keputusan eksperimen ini, nyatakan definisi secara operasi bagi osmosis.

…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
[3 marks/ 3 markah]

(g) Another experiment is carried out to study the effect of different concentrations of sucrose
solutions on the tissue of spinach strips.
The observation of the experiment is shown in Diagram 2.
Satu lagi eksperimen telah dijalankan untuk mengkaji kesan kepekatan larutan sukrosa yang
berlainan terhadap tisu jalur bayam.
Pemerhatian eksperimen ditunjukkan dalam Rajah 2.

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Classify the sucrose solutions P, Q and R.
Kelaskan larutan-larutan sukrosa P, Q dan R.

Type of solution compared to the concentration of

Types of sucrose solution cell sap of spinach
Jenis larutan sukrosa Jenis larutan berbanding dengan kepekatan sap sel
bayam

[3 marks/ 3 markah]

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SET 2 : (ACTIVITY 9.5, PAGE 158, FORM 4)
TITLE : Determining the Contents of Vitamin C In Fruit Juice
And Vegetable Juice
OBJECTIVE : To determine the contents of Vitamin C in Orange, Lemon Lime Juice.

SAMPLE QUESTION:

2 A group of students carried out an experiment to investigate the percentage of vitamin C in lemon
juice by using dichlorophenolindophenol (DCPIP) solution. The experiment was carried out using
samples of fresh lemon juice and cooked lemon juice. The students carried out the following steps:
Sekumpulan pelajar menjalankan eksperimen untuk mengkaji peratus vitamin C di dalam jus lemon
menggunakan larutan diklorofenolindofenol (DCPIP). Eksperimen dijalankan dengan menggunakan
sampel jus lemon yang segar dan jus lemon yang sudah dimasak. Pelajar tersebut melakukan
kaedah-kaedah berikut:

Step 1: 1 ml DCPIP solution was placed in a test tube.

Langkah 1: 1 ml larutan DCPIP dimasukkan ke dalam sebuah tabung uji.

Step 2: A few drops of 0.1% ascorbic acid was added to the DCPIP solution until the blue coloured
of DCPIP solution turns colourless.
Langkah 2: Beberapa titis larutan asid askorbik 0.1% ditambah ke dalam larutan DCPIP sehinggalah
larutan berwarna biru DCPIP menjadi luntur.

Diagram 1.1 shows the method used in the experiment.

Rajah 1.1 menunjukkan kaedah yang digunakan dalam eksperimen.

Diagram 1.1/ Rajah 1.1

Based on Diagram 1.1, the volume of 0.1% ascorbic acid solution to decolourise 1 ml DCPIP solution
is 1 ml.
Berdasarkan Rajah 1.1, isi padu larutan asid askorbik 0.1% yang digunakan untuk melunturkan
1 ml larutan DCPIP ialah 1 ml.

Step 3: Step 1 and 2 were repeated by using fresh lemon juice and cooked lemon juice to replace
the 0.1% ascorbic acid solution.
Langkah 3: Langkah 1 dan 2 diulang dengan menggunakan sampel jus lemon segar dan jus lemon
yang sudah dimasak bagi menggantikan larutan asid askorbik 0.1%.

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The volumes of fruit juice used to decolourise 1 ml DCPIP solution are shown in Table 1.1.
Isipadu jus buah-buahan yang digunakan untuk melunturkan 1 ml larutan DCPIP ditunjukkan
dalam Jadual 1.1.

Volume of fruit juice (ml) Volume of fruit juice

Isi padu jus buah (ml) used to decolourise
Sample of fruit juice DCPIP solution (ml)
Sampel jus buah Isipadu jus buah yang
Initial volume Final volume digunakan untuk
Isi padu awal Isi padu akhir melunturkan larutan
DCPIP (ml)

Fresh lemon juice

Jus lemon segar (i)…………………………….

Cooked lemon juice

Jus lemon yang (ii)……………………………
sudah dimasak

Table 1.1/ Jadual 1.1

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(a) Record the volumes of fruit juice that are needed to decolourise the 1 ml DCPIP solution in
the space provided.
Rekodkan isi padu jus buah yang diperlukan untuk melunturkan warna 1 ml larutan DCPIP
dalam ruang yang disediakan.
[2 marks/ 2 markah]

(b) Based on Table 1, calculate the concentration of Vitamin C in fresh lemon juice and cooked
lemon juice by using formula below:
Berdasarkan Jadual 1, kira kepekatan Vitamin C dalam jus lemon segar dan jus lemon
dimasak menggunakan formula di bawah:

Concentration of vitamin C in fruit juice

= Volume of 0.1% ascorbic acid solution X 1.0 mg cm-3
Volume of fruit juice used

Kepekatan vitamin C di dalam jus buah

= Isi padu larutan askorbik 0.1% X 1.0 mg cm-3
Isipadu jus buah yang digunakan

i. Cooked Lemon Juice / Jus lemon dimasak:

ii. Fresh Lemon Juice / Jus lemon segar:

[2 marks/ 2 markah]

(c) State one precaution that can be observed in Diagram 1.1

Nyatakan satu Langkah berjaga-jaga yang boleh diperhatikan pada Rajah 1.1

…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
[1 mark/ 1 markah]

(d) State the hypothesis for this experiment

Nyatakan hipotesis bagi eksperimen ini.

…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
[2 marks/ 2 markah]

9
 (e) State the variables for this experiment and method to handle the variable.
Nyatakan pemboleh ubah bagi experiment ini dan cara menggendalikan pemboleh ubah.

Variables Method to handle the variable

Pemboleh ubah Cara mengendalikan pemboleh ubah
Manipulated variable:
Pemboleh ubah dimanipulasikan: ……………………………………………………………………………..
……………………………………… ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
Responding variable:
Pemboleh ubah bergerak balas: ……………………………………………………………………………..
……………………………………… ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
Constant variable:
Pemboleh ubah dimalarkan: ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
……………………………………………………………………………..
………………………………………

[3 marks/ 3 markah]

(f) Based on the results of this experiment, state the operational definition for vitamin C.
Berdasarkan keputusan eksperimen ini, nyatakan definisi secara operasi bagi vitamin C.

…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
[3 marks/ 3 markah]

(g) The following list shows fruits that contain high and low contents of vitamin C.
Senarai berikut menunjukkan buah-buahan yang mengandungi kandungan vitamin C yang
tinggi dan rendah.

Pineapple Papaya Starfruit

Nenas Betik Belimbing

Orange Banana Watermelon

Oren Pisang Tembikai

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Complete Table 1.3 by classifying the fruits into groups of ‘high vitamin C fruit’ and ‘low
vitamin C fruit’.
Lengkapkan Jadual 1.3 dengan mengelaskan buah-buahan tersebut ke dalam kumpulan
‘buah berkandungan vitamin C tinggi’ dan ‘buah berkandungan vitamin C rendah’.

High content of vitamin C fruit Low content of vitamin C fruit

Buah berkandungan vitamin C tinggi Buah berkandungan vitamin C rendah

[3 marks/ 3 markah]

11
SET 3 : (ACTIVITY 9.4, PAGE 156 - 157, FORM 4)
TITLE : Studying the Energy Value of Food Sample
OBJECTIVE : To Study the Energy Value in Cashew Nuts and Groundnuts

SAMPLE QUESTION:

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 Table 1/ Jadual 1

(a) Record the increase in water temperature in Table 1.

Rekod kenaikan suhu air di dalam Jadual 1.
[3 marks/ 3 markah]
(b) Based on Table 1, calculate the energy value for bread, anchovy, and cashew nut.
Berdasarkan Jadual 1, kira nilai tenaga bagi roti, ikan bilis dan kacang gajus.

iii. Bread / Roti:

iv. Anchovy / Ikan bilis:

v. Cashew Nut / Kacang gajus:

[3 marks/ 3 markah]

(c) State one precaution that can be observed in Diagram 2.

Nyatakan satu Langkah berjaga-jaga yang boleh diperhatikan pada Rajah 2.

…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
[1 mark/ 1 markah]

16
 (d) State the hypothesis for this experiment
Nyatakan hipotesis bagi eksperimen ini.

…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
[2 marks/ 2 markah]

(e) State the variables for this experiment and method to handle the variable.
Nyatakan pemboleh ubah bagi experiment ini dan cara menggendalikan pemboleh ubah.

Variables Method to handle the variable

Pemboleh ubah Cara mengendalikan pemboleh ubah
Manipulated variable:
Pemboleh ubah dimanipulasikan: ……………………………………………………………………………..
……………………………………… ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
Responding variable:
Pemboleh ubah bergerak balas: ……………………………………………………………………………..
……………………………………… ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
Constant variable:
Pemboleh ubah dimalarkan: ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
……………………………………………………………………………..
………………………………………

[3 marks/ 3 markah]

(f) Based on the results of this experiment, state the operational definition for energy value.
Berdasarkan keputusan eksperimen ini, nyatakan definisi secara operasi bagi nilai tenaga.

…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
[3 marks/ 3 markah]

17
(g)

Based on diagram above, classify the following information according to the food class.
Berdasarkan rajah di atas, kelaskan maklumat berikut mengikut kelas makanan.

Carbohydrates Lipids Proteins

Karbohidrat Lipid Protein

[3 marks/ 3 markah]

18
SET 4 : Activity 13.1, Page 257, Form 4
TITLE : Effects of Consuming Different Volumes of Water on Urine Production
OBJECTIVE : To Study the Effect of Drinking Different Amount of Water on The
Reabsorption of Water in The Kidney.

SAMPLE QUESTION:
4 Kidney is an organ that regulates the salts and water balance in the body to maintain the blood
osmotic pressure. This process is called osmoregulation. It is controlled by two hormones that are
antidiuretic hormone (ADH) and aldosterone. The reabsorption of water and salts in the kidneys
determine the volume of urine produced.
Ginjal adalah organ yang mengawal atur keseimbangan air dan garam dalam badan supaya tekanan
osmosis darah dapat dikekalkan. Proses ini dikenali sebagai proses pengosmokawalaturan. Ianya
dikawalkan oleh dua hormon, iaitu hormon antidiuresis (ADH) dan aldosteron. Penyerapan semula
air dan garam di dalam ginjal menentukan isipadu air kencing yang dihasilkan.

An experiment was carried out to study the effect of drinking different amount of water on the
reabsorption of water in the kidney.
Satu eksperimen telah dijalankan untuk mengkaji kesan minum jumlah air yang berbeza ke atas
penyerapan semula air dalam ginjal.

A student was chosen to carry out the experiment. The student was not allowed to eat or drink one
hour before the experiment and had to empty his/her bladder before drinking 100 ml of distilled
water. After an hour, the student collects his/her urine in a measuring cylinder.
Seorang pelajar telah dipilih untuk menjalankan eksperimen tersebut. Pelajar tersebut tidak
dibenarkan makan atau minum satu jam sebelum eksperimen dan perlu mengosongkan pundi
kencing sebelum minum 100 ml air suling. Selepas satu jam, pelajar itu mengumpulkan air kencing
di dalam silinder penyukat.

The student repeated the experiment for the next four consecutive days with different volumes of
distilled water which were 200 ml, 300 ml, 400 ml and 500 ml.
Pelajar itu mengulangi eksperimen untuk empat hari berturut-turut tetapi meminum jumlah air
suling yang berbeza iaitu 200 ml, 300 ml, 400 ml dan 500 ml.

Diagram 1 shows the volume of urine collected by the student.

Rajah 1 menunjukkan isipadu air kencing yang dikumpul oleh pelajar tersebut.

Diagram 1/ Rajah 1

19
Table 1 shows the volume of urine output when the student drank different volumes of distilled
water.
Jadual 1 menunjukkan isipadu air kencing yang dihasilkan apabila pelajar itu meminum isipadu air
suling yang berbeza.

Volume of urine output (ml) Isipadu air

Volume of distilled water drunk kencing yang dihasilkan (ml)
(ml)
Isipadu air suling yang
diminum (ml)

100
………… ml

200

………… ml

300 ………… ml

20
 400
………… ml

500
………… ml

Table 1/ Jadual 1

(a) Record the volume of the urine output in Table 1.

Rekod isipadu air kencing yang dihasilkan di dalam Jadual 1
[5 marks/ 5 markah]

(b) Construct a table and record all the data collected from this experiment.
Your table should have the following titles.
Bina satu jadual dan rekodkan semua data yang dikumpul daripada
eksperimen ini.
Jadual anda hendaklah mengandungi tajuk-tajuk berikut:

a. Volume of distilled water drunk / Isipadu air suling yang diminum

b. Volume of urine output / Isipadu air kencing yang dihasilkan
c. Percentage volume of urine output / Peratus isipadu air kencing yang dihasilkan

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 [3 marks/ 3 markah]

(c) State the hypothesis for this experiment

Nyatakan hipotesis bagi eksperimen ini.

…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
[2 marks/ 2 markah]

(d) State the variables for this experiment and method to handle the variable.
Nyatakan pemboleh ubah bagi experiment ini dan cara menggendalikan pemboleh ubah.

Variables Method to handle the variable

Pemboleh ubah Cara mengendalikan pemboleh ubah
Manipulated variable:
Pemboleh ubah dimanipulasikan: ……………………………………………………………………………..
……………………………………… ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
Responding variable:
Pemboleh ubah bergerak balas: ……………………………………………………………………………..
……………………………………… ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
Constant variable:
Pemboleh ubah dimalarkan: ……………………………………………………………………………..
……………………………………………………………………………..
………………………………………
……………………………………………………………………………..
………………………………………

[3 marks/ 3 markah]

22
(e) Based on the results of this experiment, state the operational definition for osmoregulation.
Berdasarkan keputusan eksperimen ini, nyatakan definisi secara operasi bagi
pengosmokawalaturan.

…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
…………………………………………………………………………………………………………………….
[3 marks/ 3 markah]

(f) Using the list provided below, classify all the materials and apparatus correctly in Table 3
Menggunakan senarai yang disediakan di bawah, kelaskan semua bahan dan radas dalam
Jadual 3

Measuring cylinder Distilled water Beaker

Silinder penyukat Air Suling Bikar

Stopwatch Sodium Chloride Solution Watermelon

Jam randik Larutan Natrium Klorida Tembikai

Apparatus Material
Radas Bahan

[3 marks/ 3 markah]

Prepared by: Nurbahiyah binti Ab Rahman

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