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ES 120 / ES 252 / ES 600: Cooled Incubators
ES 120 / ES 252 / ES 600: Cooled Incubators
ES 120 / ES 252 / ES 600: Cooled Incubators
COOLED INCUBATORS
USER’S MANUAL
We would like to take this opportunity to thank you for preferring this Nüve product. Please
read the operating instructions carefully and keep them handy for future reference.
Please detain the packing material until you see that the unit is in good condition and it is
operating properly. If an external or internal damage is observed, contact the transportation
company immediately and report the damage. According to ICC regulations, this responsibility
belongs to the customer.
In case of a problem contact your Nüve agent for an authorized service or maintenance.
The validity of the guarantee is subject to compliance with the instructions and precautions
described in this manual.
Nüve reserves the right to improve or change the design of its products without any obligation
to modify previously manufactured products.
Information contained in this document is the property of Nüve. It may not be duplicated or
distributed without our permission.
NÜVE
SANAYİ MALZEMELERİ
İMALAT VE TİCARET A.Ş.
2
WARRANTY CERTIFICATE
1. Nüve warrants that the equipment delivered is free from defects in material and
workmanship. This warranty is given for a period of two years. The warranty period begins
from the delivery date.
2. Warranty does not apply to parts normally consumed during operation or general
maintenance or any adjustments described in the operating instructions provided with the
instrument.
3. Nüve does not accept any liability in case where the goods are not used in accordance with
their proper intent.
4. The warranty may not be claimed for damages incurred during the shipment, for damages
resulting from improper handling or use, abuse, fire, liquid spillage, tampering or
unauthorized repairs by any persons, use of defective or incompatible accessories, exposure
to abnormally corrosive conditions, use of the product in non-standard environmental
conditions, including but not limited to failure to meet requirements of ambient
temperature, lubrication, humidity or magnetic field influences, from the defects in
maintenance, negligence, bad functioning of auxiliary equipment, in the case of force
majeure or accident and incorrect power supply.
5. Any injury, loss or damage caused; due to a failure resulting from negligence of the
instructions given in this manual; is beyond the scope of the warranty conditions.
To register your warranty online, please visit our webpage www.nuve.com.tr and fill in the
“Warranty Registration Form”.
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CONTENT
Page
1. INTRODUCTION ........................................................................................................................ 5
1.1. USE AND FUNCTION .......................................................................................................... 5
2. TECHNICAL SPECIFICATIONS .................................................................................................... 6
2.1. TECHNICAL SPECIFICATIONS TABLE ................................................................................... 6
2.2. ACCESSORIES ..................................................................................................................... 6
3. PRECAUTIONS AND LIMITATIONS ON USE ............................................................................... 7
4. SYMBOLS .................................................................................................................................. 7
5. INSTALLATION .......................................................................................................................... 8
5.1. ENVIRONMENTAL CONDITIONS ........................................................................................ 8
5.2. HANDLING AND TRANSPORTATION .................................................................................. 8
5.3. UNPACKING ....................................................................................................................... 8
5.4. MAINS SUPPLY ................................................................................................................... 8
5.5. POSITIONING ..................................................................................................................... 9
5.6. GENERAL PRESENTATION .................................................................................................. 9
5.7. CONTROL PANEL .............................................................................................................. 10
5.8. PRIOR TO OPERATION ..................................................................................................... 12
6. OPERATING PRINCIPLES ......................................................................................................... 12
6.1. PROGRAMMING .............................................................................................................. 12
6.2. COMPLETION OF THE OPERATION .................................................................................. 15
6.3. MENUS ............................................................................................................................. 15
6.3.1. SETTINGS .................................................................................................................. 15
6.3.2. MEMORY .................................................................................................................. 17
7. PERIODIC MAINTENANCE AND CLEANING ............................................................................. 18
7.1. PERIODIC MAINTENANCE ................................................................................................ 18
7.2. CLEANING ........................................................................................................................ 18
8. DISPOSAL MANAGEMENT CONCEPT ...................................................................................... 19
9. TROUBLESHOOTING ............................................................................................................... 19
9.1. ERROR CODES .................................................................................................................. 19
9.2. FUSE REPLACEMENT ........................................................................................................ 20
10. OPTIONS .............................................................................................................................. 20
10.1. COMMUNICATION UNIT .............................................................................................. 20
10.2. EXTERNAL MEMORY (USB STICK) ............................................................................. 20
10.3. AlerText™ GSM MODULE ............................................................................................. 21
10.3.1. SMS ....................................................................................................................... 22
10.4. E-MAIL ...................................................................................................................... 24
11. ELECTRICAL CIRCUIT DIAGRAM ........................................................................................... 26
11.1. ES 120/252 ELECTRICAL CIRCUIT DIAGRAM ................................................................ 26
11.2. ES 600 ELECTRICAL CIRCUIT DIAGRAM ........................................................................ 27
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1. INTRODUCTION
1.1. USE AND FUNCTION
ES 120, ES 252 and ES 600 Cooled Incubators are designed for freezing, incubation, drying,
heating, different types of tests and long storage in the fields of biology, zoology, botany and in
the quality control and R&D laboratories in industries as pharmaceutical, food and cosmetics.
The Cooled Incubators operate in temperature range between -10°C and 60°C and keeps the
temperature stable within the given tolerances. ES series Cooled Incubators are safely operated
via N-SmArt™ microprocessor control system. Adjustable safety thermostat offers an additional
safety for your operations.
Stable and homogeneous temperature in the useful volume is provided by means of the forced
air circulation through the stainless steel heater and the evaporator through which refrigerant
circulates. The insulation proportional to the dimensions of the incubator not only provides a
homogenous temperature distribution but also saves energy.
A well-insulated glass window and switchable interior lighting permit samples to be observed
throughout the incubation process. Fluorescent lamb is used for interior lighting.
ES series have audible and visual alarm systems for open door more than 2 minutes, power
failure, high and low temperature conditions, temperature sensor failure and other faults
associated with the microprocessor control system. ES series store last 50 failures in the
memory and these errors can be followed on the display. Optional Ethernet connection feature
enables email sending to five different e-mail addresses in case of any error. ES series offer
AlerText™ GSM module as an option and SMS can be sent to five different telephone numbers
in case of error.
ES series incubators have internal memory which can store temperature values for 10 years. In
addition to internal memory, temperature values can be recorded in USB stick with
communication unit as an option.
If the warnings mentioned in this manual are not considered, NUVE will not be responsible
from their results.
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1. TECHNICAL SPECIFICATIONS
2.1. TECHNICAL SPECIFICATIONS TABLE
TECHNICAL SPECIFICATIONS ES 120 ES 252 ES 600
Temperature Range -10°C / +60°C
Temperature Sensor Pt-100
Temperature Set and Reading Sensitivity 0.1°C
Temperature Fluctuation (at 37°C) 0.5°C 1°C
Timer 1 min – 99.9 hours / Hold position
Number of Program and Step 20 / 20
Safety System Gas expansion safety thermostat (0 – 90°C)
Defrost Automatic
Refrigerant R134a R404a
Power Supply 230 V / 50-60 Hz
Number of Shelves (Standard/Max) 2/10 3/20 2/25
Power Consumption 800 W 1250 W 2000W
Useful Volume (liter) 120 252 600
Internal Material Stainless Steel
External Material Epoxy-polyester powder coated stainless steel
Control System N-SmArt™ Programmable Microprocessor Control
System
Temperature Indicator Colorful LCD
Internal Memory 10-year memory
USB Optional
Ethernet Optional
RS 232 Optional
Internal Dimensions (WxDxH) (mm) 480x545x465 475x545x1110 735x640x1210
External Dimensions (WxDxH) (mm) 655x745x1020 655x745x1855 925x850x1965
2.2. ACCESSORIES
A 08 190 Communication Unit
A 08 191 AlerText™ GSM alarm module
A 08 195 NüveCloser™ Software CD with 3 m. RS 232 cable
R 01 130 Shelf for ES 120 and ES 252
K 23 040 Shelf Carrier for ES 120/252
R 01 165 Shelf for ES 600
K 23 064 Shelf Carrier for ES 600
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2. PRECAUTIONS AND LIMITATIONS ON USE
The user shall pay attention to the following:
• Do not operate the instrument for purposes other than its main purpose.
• The instrument should only be used by authorized and trained staff after the instruction
manual has been read carefully. Only authorized technical staff can handle the product in
case of a failure.
• Only original spare parts and original accessories supplied by Nüve should be used.
• Correctly grounded power supply should be used.
• The set temperature should not destroy the structure of the samples without user’s notice.
• The boiling points of the samples should be higher than the set temperature.
• The freezing points of the samples should be lower than the set the temperature.
• The samples which may liquefy and expand should not be in a sealed container.
• Liquids which may expand during heating should not overflow from their containers.
• The safety thermostat should be adjusted to the temperature higher than the set
temperature.
• There should not be any material in the chamber that can damage to the device.
• The materials which will be subject to heating or drying should not be combustible,
explosive, heat susceptible, flammable, adhesive and fusible.
• Ensure that the vapors and gases generated during the operation are not harmful to human
health and flammable or explosive.
3. SYMBOLS
Symbol in the operating instructions:
This symbol refers to safety relevant warnings and indicates possibly dangerous
situations. The non-adherence to these warnings can lead to material damage and injury
to personal.
Earthed Wall Socket ES 120 / ES 252 Fuses (2 x 10A) ES 600 Fuses (2 x 13A)
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4. INSTALLATION
4.1. ENVIRONMENTAL CONDITIONS
All handling and transportation must be carried out by using proper equipment and experienced
staff. The instrument must be supported underneath and never be turned upside down.
4.3. UNPACKING
Remove the cardboard box packing and the second nylon wrapping around the instrument.
Ensure that no damage occurred during transportation. The below mentioned are provided with
the instrument, please check them;
Please make sure that the supplied mains matches the required power ratings which are written
on the name of plate of the instrument located at the back of the instrument.
A supply fitted with a circuit breaker should be used for protection against indirect
contact in case of isolation fault.
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4.5. POSITIONING
EXTERNAL SENSOR
ACCESS PORT
DOOR OBSERVATION
WINDOW
COMMUNICATION
CONTROL PANEL
UNIT (OPTIONAL)
POWER SWITCH
Figure 1
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Figure 2
Figure 3
The functions of F1, F2, F3 and F4 buttons depend on the meaning of the corresponding symbol
appearing on the display. The following table shows the meaning of these symbols.
This symbol denotes the menu. You can enter the menu by pushing the button
corresponding to this symbol.
This symbol denotes the settings page. You can enter the settings page by pushing
the button corresponding to this symbol.
This symbol denotes the value increase key. You can increase the value by
pushing the button corresponding to this symbol while adjusting the numerical
values such as temperature or password.
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This symbol denotes the value decrease key. You can decrease the value by
pushing the button corresponding to this symbol while adjusting the numerical
values such as temperature or password.
This symbol denotes the left key. It appears on the graph screen. The next graph
appears when the button corresponding to this symbol is pushed.
1) This symbol denotes the right key when the right and left keys appears on the
same screen. The previous graph is passed when the button corresponding to this
symbol is pushed.
2) It denotes the tab button when the left and the right keys do not appear on the
same screen.
This symbol denotes backspace. You can return the previous page or exit from the
page by pushing the button corresponding to this symbol.
The symbol is on the green button which is used to start the program.
The symbol is on the red button which is used to stop the program.
The key corresponding to this symbol is used to turn the illumination on or off. If
the key is green, it shows that the lamp is off. The lamp goes on when the key is
pushed and the key turns red. Lamp goes off when the red key is pushed.
This symbol denotes the explanation of the error codes. You can learn detailed
explanation about the error by pushing the button corresponding to this symbol.
It appears in case of failures.
This symbol denotes the alarm mute key. You can mute the audible alarm by
pushing the button corresponding to this symbol when the alarm is activated in
case of error.
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4.8. PRIOR TO OPERATION
5. OPERATING PRINCIPLES
5.1. PROGRAMMING
At most 70% of the surface area of the shelves should be used in order to obtain a
uniform temperature distribution.
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The step settings screen on the left appears,
when enter key (F4) is pushed when the selection
is on program on the operations settings screen.
On the upper line the program number whose
steps are set is shown. Increase (F1) and decrease
(F2) keys are used to change the selection value.
Enter key (F4) is used to change the selection.
The settings for the specific step number are displayed when the step number is changed by
using increase (F1) and decrease (F2) keys.
Back key (F3) is used to exit the screen. Push back key (F3) to return the operation settings
screen.
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12- Selection is on Starting Step No. it shows the step number which starts to run when the
program starts. Choose the step number which starts to run by using increase (F1) and
decrease (F2) keys and push tab key (F3).
13- Selection is on Repeated Operation. Set repetition number by using by using increase (F1)
and decrease (F2) keys and push tab key (F3).
14- Selection is on Stop at Power Failure. It can be chosen as ON or OFF by using increase (F1)
and decrease (F2) keys.
If Stop at Power Failure is ON;
• The program will be interrupted in case of power failure. When the power failure is over,
‘Power Failure’ error appears on the screen.
If Stop at Power Failure is OFF;
• The program will continue to run in case of power failure.
o If the temperature is in the temperature alarm range, there will be notification
regarding power failure. Push question mark key (F3) to see the notification.
o If the temperature is out of the temperature alarm range, ‘Out of Temperature
Range’ error appears.
15- Push tab key (F3). The selection is on Delay. It can be set from 0 to 99 hours 59 minutes by
using increase (F1) and decrease (F2) keys. The program which is started starts to run after
the delay time ends.
Figure 4
N-SmArt™ microprocessor control system offers the option to follow the running program on a
graph. Follow the steps below to monitor the program on a graph:
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5.2. COMPLETION OF THE OPERATION
If the unit is in START position when the door is open, it will keep operating and the
heaters will be over-heated. This situation may result in irreparable damage in the
heaters and other components.
5.3. MENUS
When menu key (F1) is pushed on the working screen, password query screen appears. Enter
password by using increase (F1) and decrease (F2) keys and push enter key (F4). The password is
user password and it can be set on settings menu (See Section 6.3.1). The password is 0000 for
the first use.
5.3.1. SETTINGS
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“Date/Time Settings” submenu is selected on
the settings page by the value increase (F1) and
decrease (F2) keys. The page shown on the left
appears when enter key (F4) is pushed. Time is
shown in the format of “hour:minute” and the
cursor is on hour part when “Date/Time
Settings” page comes to the screen. Hour is
adjusted by pushing the value increase (F1) and
decrease (F2) keys. Push enter key (F4) to
continue to adjust. If you want to exit this page without any change, push backspace key (F3).
“Select Language” submenu is selected on the
settings page by using the value increase (F1)
and decrease (F2) keys. The page on the left
appears when enter key (F4) is pushed. The
language of the control panel can be set as
Turkish, English, French, Russian or Spanish.
Push the value increase (F1) and decrease (F2)
keys to select the language and then push the
enter key (F4) to save the selection. If you want
to exit this page without any change, push the
backspace key (F3).
“Screen Saver Settings” submenu is selected
on the settings page by using the value
increase (F1) and decrease (F2) keys. The page
on the left appears when enter key (F4) is
pushed. Screen saver can be on or off by
pushing the value increase (F1) and decrease
(F2). If the option of “open” is selected,
activation time of screen saver is adjusted on
screen saver time in minutes. If you want to
exit this page without any change, push the
backspace key (F3).
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the backspace key (F3).
5.3.2. MEMORY
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When the temperature sensor is broken, “–“appears in the temperature column since
temperature cannot be reading by the sensor.
“History (GRAPH)” submenu is selected on the memory page by the value increase (F1) and
decrease (F2) keys. “Select Record Date” page appears when enter key (F4) is pushed. Enter a
date to analyze corresponding operation and then push the enter key (F4). You can analyze the
data in the graph. The data belonging to the other dates can be followed by using left (F2) and
right (F3) keys. Push the backspace key (F4) to return the previous page.
Values are recorded every one hour and while program is running.
6.2. CLEANING
• Prior to cleaning please unplug the device and clean the device at the room temperature.
• After every operation wipe down the chamber surface to remove any undesirable effects of
the operation as spillage.
• A piece of wet cloth may be used in order to remove the dust and dirt from the device.
• Mild detergent use is recommended to remove difficult dust and dirt.
• Clean the condenser filter regularly.
• Please be aware of the undesirable effects of the chemicals and be careful while applying
them.
• Protect the device against rust from outside. Please clean the rust as soon as possible when
it is detected.
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7. DISPOSAL MANAGEMENT CONCEPT
The currently valid local regulations governing disposal must be observed. It is in the
responsibility of the user to arrange proper disposal of the individual components.
All parts which may comprise potentially infectious materials have to be disinfected by suitable
validated procedures (autoclaving, chemical treatment) prior to disposal. Applicable local
regulations for disposal have to be carefully observed.
The instruments and electronic accessories (without batteries, power packs etc.) must be
disposed of according to the regulations for the disposal of electronic components.
Batteries, power packs and similar power source have to be dismounted from electric/electronic
parts and disposed of in accordance with applicable local regulations.
8. TROUBLESHOOTING
If the device fails to operate, please check the followings:
• The power switch is on;
• The fuse is sound;
• The plug is plugged-in properly;
• The plug is not defective;
• The mains supply is present.
If the Cooled Incubator does not heat, check the followings;
• The program has started.
• The safety thermostat is set at a temperature higher than the set temperature.
If the temperature exceeds the set value, check the followings;
• The internal circulation fan operates.
If the cooled incubator does not cool, check the followings;
• The internal circulation fan operates,
• The ambient temperature does not exceed 20 (± 5)°C limits.
EoFF: This error code shows power failure. If Stop at Power Failure is ON, the program is
interrupted in case of power failure. When the power failure is over, ‘Power Failure’ error
appears on the screen.
After power interruption, push the key corresponding to question mark (F3) in order
to see the time when power interruption occurs.
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Err2: It appears when there is a communication error between display and main PCB.
Err6: It shows that the temperature in the chamber is not in the temperature alarm range.
Err7: It appears when software version is not compatible. Push the F3 key corresponding to the
question mark to see the explanation. Inform the authorized NÜVE agent regarding the error
explanation.
A list consisting of the codes of errors can be accessed from the memory page (See
section 6.3.2). ‘EoFF’, ‘Err3’, ‘Err5’ and ‘Err6’ do not appear on working screen and
they are only shown in the list of the error history.
If an error occurs, please contact to an authorized Nüve agent to seek technical help.
9. OPTIONS
9.1. COMMUNICATION UNIT
There are 4 outlets on communication unit (Figure 6). The function of each outlet is explained in
the following sections.
USB PORT-1
USB PORT-2
ETHERNET
RS 232
In case of error, SMS can be sent to five different phone numbers by GSM module as an option.
LED
Figure 7
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ANTENNA INLET
SMS LED
POWER LED
SIM CARD INLET
Figure 8 Figure 9
Please carry out the following steps for connection of GSM module:
SIM card is provided by the user. The cost of SIM card and SMS differs according to
the GSM providers and all charges will be covered by the user.
SIM card which will be used for GSM module should not have PIN code.
9.3.1. SMS
In order to use SMS, optional GSM module is mandatory to have. Refer to Section
10.1 for information regarding GSM module connection.
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“SMS” submenu is selected on the menu page by
the value increase (F1) and decrease (F2) keys.
The page on the left appears when enter key (F4)
is pushed. Submenus on SMS page is selected by
using increase (F1) and decrease (F2) keys.
Selected submenu can be adjusted by using enter
key (F4). If you want to exit the page, push the
backspace key (F3).
• Activation or deactivation of SMS function can be adjusted on “SMS” submenu. If you want
to activate this function, choose “on” by pushing the enter key (F4). If you want to
deactivate SMS function, choose “Off” by pushing the enter key (F4).
• “Repeat time” is the frequency of sending SMS. The user is notified again by sending SMS if
the failure still continues. Repeat time can be adjusted to 8 hours, 16 hours and 24 hours by
pushing enter key (F4).
• SMS can be sent to 5 different mobile phones. According to demand, 1st, 2nd and 3rd
numbers can be users’ numbers and 4th and 5th numbers can be technical service staffs’
numbers.
• Enter country code before the phone numbers.
• A cursor flashes under a digit which means you can set this digit of the number. Each digit of
phone numbers is entered one by one. The first digit of the phone number is entered by
pushing value increase (F1) and decrease keys (F2) and tab key (F3) is pushed to enter the
second digit. The second number is entered pushing value increase (F1) and decrease keys
(F2) and tab key (F3) is pushed to enter the next digit. After all digits of the phone number
are entered completely by this way, push the enter key (F4) to enter the next phone
number.
• Tab key (F3) is used for changeover from one digit of phone number to another.
• After all phone numbers from “Phone 1” to “Service 2” is entered as mentioned above, push
the enter key (F4) and return to main page.
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9.4. E-MAIL
“E-Mail” submenu is selected on the menu
page by the value increase (F1) and decrease
(F2) keys. The page shown on the left appears
when enter key (F4) is pushed. The choice of
submenus on e-mail page is changed by
pushing increase (F1) and decrease (F2) keys.
After choosing a submenu, the adjustments for
this submenu is made by pushing enter key
(F4). If you want to exit this page, push the
backspace key (F3).
• Activation or deactivation of e-mail function can be adjusted from “E-mail” submenu. If you
want to activate this function, choose “on” by pushing the enter key (F4). If you want to
deactivate e-mail function, choose “off” by pushing the enter key (F4).
Ethernet settings should be adjusted by technical service staff for the first usage.
Otherwise, this function does not work.
• Each character of e-mail addresses is typed one by one. The first character is entered by
pushing value increase (F1) and decrease keys (F2) and tab key (F3) is pushed to enter the
second character. The second character is written by pushing value increase (F1) and
decrease keys (F2) and tab key (F3) is pushed to enter the next character. After all characters
are written completely in this way, push the enter key (F4) to enter the mail server. After
mail server is written in the same way, push the enter key (F4) to write next e-mail address.
After third email address is written, push enter key (F4) to enter other e-mail addresses.
• After all e-mail addresses are written as mentioned above, push enter key (F4) and return to
main page.
• Tab key (F3) is used for changeover from one character of address to another.
24
“Login” is selected on the e-mail page by the
value increase (F1) and decrease (F2) keys. The
page on the left appears when enter key (F4) is
pushed. Each character of e-mail addresses is
written one by one. The first character is entered
by pushing value increase (F1) and decrease keys
(F2) and tab key (F3) is pushed to enter the next
character. The second character is entered by
pushing value increase (F1) and decrease keys
(F2) and tab key (F3) is pushed to enter the next character. After all characters of user’s name
are entered by this way, push enter key (F4) to enter the mail server of the e-mail address. After
mail server is entered in the same way, push enter key (F4) to pass the “password” submenu.
Enter the password and push enter key (F4).
The port provided by internet server is entered on the part of “Port”. After port is entered,
enter key (F4) is pushed to return the main menu. Tab key (F3) is used for changes from one
character of address to another.
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10. ELECTRICAL CIRCUIT DIAGRAM
10.1. ES 120 / ES 252 ELECTRICAL CIRCUIT DIAGRAM
26
10.2. ES 600 ELECTRICAL CIRCUIT DIAGRAM
27