Primary Metabolites

MOLISCH’S TEST
Add 2-3ml of aqueous extract in then take a look at tube. Then, upload some drops of alpha-
naphthol answer in alcohol. The colour of the answer may be yellow shade. After that, shake
it and upload attention of H2SO4 which the end result of the answer becomes superb and the
shade of the answer will alternate from yellow shade to inexperienced shade. The end result
of the answer shade turns from yellow to inexperienced shade. The take a look at grew to
become out to be superb.
BENEDICTS TEST:
Firstly, blend the identical extent of Benedict’s reagent and additionally take a look at
answers withinside the take a look at tube. After done, warmth in boiling water tub tub for
five minutes. The end result of the answer shade is mild inexperienced. No shade
adjustments. The effects for benedict's take a look at are negative
MILLON’S TEST:
Firstly, blend three ml pattern with five ml Millon’s reagent. Then, take a look at it whilst
precipitate. After that, heat it and precipitate which the answer will become brick crimson or
the precipitate dissolving will supply crimson coloured answer. The end result of the answer
shade is yellow. After heating it suggests a crimson line. The take a look at grew to become
out to be superb.
TESTS FOR FAT AND OILS:
Filter paper receives completely stained with oils will superb. And adjustments to little little
bit of darkish blue It superb. And adjustments to little little bit of darkish blue

Secondary Metabolites
Test for Flavonoids
Sulfuric acid Test When sulphuric acid (sixty-six percentage or eighty percentage) is
delivered to it, flavones and flavanols dissolve and a deep yellow answer result. Red or red-
bluish answers are shaped via way of means of chalcones and aurones. Flavones are
answerable for the colour’s orange and red. Add a small quantity of lead acetate approach to
the residue. Precipitation is yellow in colour. The very last statement turning into mild
inexperienced with down sun sunglasses brown colour. (negative)
Test for Alkaloids
Dragendorff's Test Add some drops of Dragendorff's reagent to 2-three mL filtrate. Formed
an orange brown. Mayer's Test Precipitate is produced with the aid of using blending 2-three
mL filtrate with some drops of Mayer's reagent. The very last check for tannins statement
grew to become barely to darkish blue coluar(positive).
Test for Tannins and phenolic
Compounds 5 % Cl3 answer: Deep blue-black colour. Acetic acid answer is a vivid crimson
liquid. The remark is orange brown colour(positive).