International Standard 5564

INTERNATIONAL ORGANIZATION FOR STANDARDIZATIONWIE~YHAPOAHAR OPTAHHSALWIR fl0 CTAH~PTM3ALplM~RGANISATION INTERNATIONALE DE NORMALISATION

Black pepper and white pepper, whole or ground -

Determination of piperine content - Spectrophotometric
method
Pokre nok et pokre blanc, entier ou en poudre - DtSterrnination de Ia teneur en pipkine - Methode spectropho tomhique

First edition - 1982-11-15

iTeh STANDARD PREVIEW
(standards.iteh.ai)
ISO 5564:1982
https://standards.iteh.ai/catalog/standards/sist/4ba9bb3d-7ced-44d2-8f39-
058ad0d0c289/iso-5564-1982

iz
- UDC 633.841: 543.8 Ref. No. ISO 55644982 (E)

Descriptors : agricultural products, spices, pepper, tests, spectrophotometric analysis.

Price based on 2 pages

Forewoad
ISO (the International Organization for Standardization) is a worldwide federation of
national Standards institutes (lS0 member bodies). The work of developing Inter-
national Standards is carried out through ISO technical committees. Every member
body interested in a subject for which a technical committee has been set up has the
right to be represented on that committee. International organizations, governmental
and non-governmental, in liaison with ISO, also take part in the work.

Draft International Standards adopted by the technical committees are circulated to

the member bodies for approval before their acceptance as International Standards by
the ISO Council.

International Standard iTeh STANDARD PREVIEW

ISO 5564 was developed by Technical Committee ISO/TC 34,
dlgricultu~alfoodpaoduct~, and was circulated to the member bodies in February 1981.
(standards.iteh.ai)
lt has been approved by the member bodies of the following countries :
ISO 5564:1982
Austria https://standards.iteh.ai/catalog/standards/sist/4ba9bb3d-7ced-44d2-8f39-
Italy Portugal
Canada Kenya 058ad0d0c289/iso-5564-1982
Romania
Czechoslovakia Korea, Dem. P. Rep. sf South Africa, Rep. of
Egypt, Arab Rep. of Korea, Rep. of Spain
Ethiopia Malaysia Sri Lanka
France Netherlands Thailand
Germany, F. R. New Zealand Turkey
Hungary Peru United Kingdom
lndia Philippines USSR
lreland Poland Y ugoslavia

No member body expressed disapproval of the document.

0 International Organkation for Standardkation, 1982

Printed in Switzerland
INTERNATIONAL STANDARD ISO 5564-1982 (E)

Black pepper and white epper, whole’or gr,ound -

Determination of pi erine content - Spectrophotometric
method

0 Introduction 5 Reagent

on a number of international
iTeh STANDARD 5.1PREVIEW
The method described in this international
collaborative
Ethanol, 96 % WV.
Standard is based
studies carried out
(standards.iteh.ai)
over a Iong period of time. Necessarily, the method seeks to
optimize a number of variables in an attempt to define pro- 6 Apparatus
cedures and provide a common measure of the pungency of
ISO 5564:1982
PePPer- Usual laboratory equipment, and in particular
https://standards.iteh.ai/catalog/standards/sist/4ba9bb3d-7ced-44d2-8f39-
058ad0d0c289/iso-5564-1982
6.1 Extraction apparatus, comprising the following com-
1 Scope and field of application ponents joined together by means of ground-glass joints.

This International Standard specifies a spectrophotometric

method for the determination of the piperine content of black 6.1.1 Flask, round-bottomed, of capacity 100 ml.
or white pepper (Pipernigrum L.), in whole or in ground form.
Kl.2 Reflux condenser.

2 References 6.1.3 Anti-bumping granules : glass beads.

ISO 939, Spices and condiments - Determination of moisture

content - En trainmen t me thod. 6.2 Qne-mark wolumetric flasks, of capacities 25, 50 and
100 ml, complying with the requirements of ISO 1042, and
ISO 948, Spices and condiments - Sampling. wrapped in aluminium foil or black tape (6.4).

ISO 2825, Spices and condiments - Reparation of a ground

Sample for analysis.
6.3 Filter Paper.

6.4 Aluminium foil or black tape.

3 Definition

3.1 piperine content : The quantity of pungent compounds 6.5 Pipettes, one-mark, of capacity 5 ml, complying with
(piperine and its analogues) in the product, determined by the requirements of ISO 648.
the procedure specified in this International Standard, and ex-
pressed as a percentage by mass. 6.6 Spectrophotometer, capable of accurately measuring
absorbances at 343 nm, and provided with a pair sf matched
silica cells, or cells transparent to ultraviolet radiation, of Optical
4 Principle path length 1 cm.

Extraction of the pungent compounds with ethanol and spec-

trophotometric measurement at 343 nm. 6.7 Analytical balance.

1
ISO 55644982 (EI

7 Sampling Measure the absorbance (Al of this Solution at 343 nm, by

means sf the spectrophotometer (6.61, using, as the reference
Sample the material by the method described in ISO 948. liquid, the ethanol (5.1).

8 Procedure 9 Expression of resuits

NOTE - It is essential during the operations described below, and The piperine content, expressed as a percentage bY mass on
whiist the piperine is in soiution, to avoid exposure to light. The the dry basis, is equal to
isomerisation of piperine is catalysed by sunlight, and, therefore,
during extraction and storage of the stock solution, iight should be A 50 25 100 100
-x-x-x
excluded by wrapping the flasks with aluminium foil or black tape x5 5 m 100~H
J %
(sec 6.2).
lcm

81. Preparation of test Sample

m is the mass, in grams, of the test Portion (8.2);
Prepare the test Sample as described in ISO 2825.
H is the moisture content, expressed as a percentage by
mass, of the test Sample, determined by the method
8.2 Test Portion
specified in ISO 939;

Weigh, to the nearest 0,000 1 g, about 0,5 g of the’test Sample A is the absorbance of the final test Solution; a
and transfer it to the round-bottomed flask (6.1 .l).
A1 %
is the absorbance at 343 nm of a 1 %
lcm
8.3 Determination Solution of piperine in a cell of Optical path length 1 cm,
which equals 1 238.
Transfer about 50 ml of the ethanol (5.1) to the flask (6.1.1) and
add a few anti-bumping iTeh STANDARD PREVIEW
granules (6.1.3). Connect the con-
denser (6.1.2) and, in a dark place (sec the introductory note to 10 Test report
(standards.iteh.ai)
clause 81, boil under reflux for 3 h. Allow to cool and filter the
Solution into a 160 ml volumetric flask (6.2). Wash the extrac- The test report shall Show the method used and the result ob-
tion flask and filter Paper with successive 10 ml portions of the tained. lt shall also mention any operating conditions not
ethanol and use the washings to make up the volume in the ISO 5564:1982 specified in this International Standard, or regarded as op-
https://standards.iteh.ai/catalog/standards/sist/4ba9bb3d-7ced-44d2-8f39-
volumetric flask to the mark. Transfer, by means of a pipette tional, together with any circumstances that may have influenc-
(6.51, a 5 ml aliquot Portion of this Solution to a058ad0d0c289/iso-5564-1982
50 ml ed the result.
volumetric flask (6.2) and make up to the mark with ethanol.
Transfer, by means of a pipette (6.51, 5 ml of this Solution into a The test report shall include all the information necessary for
25 ml volumetric flask (6.2) and again make up to the mark with the complete identification of the Sample.
ethanol.