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Efficacy of Guyabano Leaves Extract (Annona Muricata) As A Syrup To Reduce Hyperglycemic Effect
Efficacy of Guyabano Leaves Extract (Annona Muricata) As A Syrup To Reduce Hyperglycemic Effect
ABSTRACT
This research study conducted to the efficacy of Guyabano Leaves (Annona Muricata) As
Guyabano leaves were collected from the researcher’s house. Albino rats are the test
organisms used in the experiment and is purchased from a registered pet shop in San Carlos City
Pangasinan.
The guyabano leaves were pulverized in preparation for the different treatments: for
treatment 1, there will be 75% of guyabano leaves extract and 25% of artificial sweetener, for
treatment 2, there will be 50% of guyabano leaves extract and 50% of artificial sweetener, for
The experimentation takes place at the VMU College of Pharmacy Laboratory with the
The blood samples were used to determine the decreasing of platelet of the eighteen white rats.
The results of the study showed that treatment 3 is the most feasible treatment in reducing
the blood pressure of the white rats. There is a significant difference between the initial and final
blood pressure level of white rats before and after the application of treatment. There is a
significant difference among the treatments in terms of effectiveness in reducing blood sugar
victim of white rats. In conclusion, guyabano leaves extract can be used as syrup to reduce blood
sugar.
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Chapter 1
Introduction
Background of study:
One of the most common problems in today's world is the high number of population that
have high blood sugar. Blood sugar control is at the center of any diabetes treatment plan. High
blood sugar, or hyperglycemia, is a major concern, and can affect people with both type one and
type two diabetes. Frequent or ongoing high blood sugar can cause damage to your nerves, blood
vessels, and organs. It can also lead to other serious conditions. People with type two diabetes
are prone to a build-up of acids in the blood called ketoacidosis. If a person has type 2 diabetes
or if he is at risk for it, extremely high blood sugar can lead to a potentially deadly condition in
which the human body can’t process sugar. It's called hyperglycemic hyperosmolar non ketotic
syndrome (HHNS). A person will pee more often at first, and then less often later on, but his
urine may become dark and he could get severely dehydrated. It's important to treat symptoms of
The body of a person with Type 1 diabetes needs insulin because their pancreas is
damaged in a way that will not produce enough insulin as needed. Insulin is a chemical
compound used to break down sugar (glucose) in the bloodstream. Those with type 1 diabetes
must work with their doctor to find their correct dosage of insulin, because different individuals
have different reactions to various types of insulin, and because some individuals with this type
In general, people with diabetes should strive to be physically fit. Physical exercise has the
effect of lowering the body's glucose levels - sometimes for as long as 24 hours. Because the
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
most harmful effects of diabetes are caused by elevated glucose levels (blood sugar "spikes"),
exercise after eating is a valuable tool that uses sugar naturally and allows people with diabetes
If you have Type 2 diabetes, your body is able to produce some insulin, as opposed to
none at all, but has a diminished capacity for producing insulin or is not able to use the chemical
correctly. Because of this crucial difference, Type 2 diabetes symptoms can be more mild than
Type 1 symptoms, and have a more gradual onset, and can require less drastic treatments.
Type 2 diabetes is known to be a progressive disease. This means that it can get worse
over time. This is thought to be because the body's cells responsible for producing insulin get
"worn out" from having to work extra hard in people with Type 2 diabetes. As a result, cases of
Type 2 diabetes that once required relatively minor treatment options can eventually require
more drastic treatments, including insulin therapy, after several years. This is often not due to
Those with Type 1 diabetes are often instructed to check their blood sugar three or
more times a day. This is often done before or after certain meals, before or after exercise, before
bed, and even during the night. If you are sick or are taking a new medication, you may need to
Those with Type 2 diabetes, on the other hand, usually don't have to check their
blood sugar quite as often - they may be instructed to check their levels once or more per day. In
cases where Type 2 diabetes can be managed with non-insulin medications or diet and exercise
alone, your doctor may not even require you to check your blood sugar every day. A1C tests
work by analyzing a molecule in your blood called hemoglobin. When glucose enters your
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
blood, some of it binds to these hemoglobin molecules. Because hemoglobin molecules usually
live for about 3 months, analyzing the percentage of hemoglobin molecules that are bound to
glucose can paint a picture of how high blood sugar levels were over the past few months.
This study will help in reducing the blood sugar in the human body by producing a syrup
that is made out from Guyabano leaves. It is a known fact that Guyabano leaves has its ability to
treat high blood sugar. A group of scientist published their report in the 2008 African Journal of
Traditional Complementary and Alternative Medicine that looked into the effect of Guyabano
leaves extracts in diabetes melitus-induced rats. The test subject was induced with diabetes
melitus and then fed with the extracts of Guyabano leaves for an extended period. The study
reported positive effects of leveling down the blood sugar of the animal test subjects. It’s worth
to note that there haven’t been any studies that research the effects of Guyabano leaves tea and
extract on humans in regard to diabetes. However, people in Southeast Asia have been using the
leaves tea as herbal medication for diabetes and continually reporting positive effects, especially
This study will determine The Efficacy of Guyabano Leaves (Annona Muricata) as Syrup
to Reduce Blood sugar. Specifically, this study aims to seek the answers to the following
questions.
1. Is the Guyabano Leaves (Annona Muricata) has an effect to reduce blood sugar?
3. How long it takes before the Guyabano Leaves reduce the blood sugar?
Alternative Hypothesis
Null Hypothesis
1. Guyabano Leaves (Annona Muricata) is not effective to reduce high blood sugar.
This study aimed to test the Guyabano leaves (Annona Muricata) as Syrup to reduce blood
sugar.
1. To determine the significant effect of the different treatment to reduce blood sugar.
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
treatments in trials.
The Efficiency of Guyabano Leaves (Annona Muricata) as Syrup to Reduce Blood sugar
To the People who can’t afford the commercial medicine for reducing blood sugar, it
helps people to buy cheaper cure for the high blood sugar
To the Department of Health, that is finding more sources of different medicines. We can
To the Community, to let them know that Guyabano (Annona Muricata) leaves can be
made as Syrup to cure or to reduce blood sugar. To let the people in the community know that
To the Environment, that provides our needed resources and natural systems that keep all
This study had been conducted to determine the efficiency of Guyabano leaves (Annona
Muricata) as syrup to reduced blood sugar. We have conducted this research in Alaminos City
National High School Science Laboratory. This research has taken five days. This is used a
For clearer understanding of this study, the following terms and variable are defined in
which there are high blood sugar levels over a prolonged period. Symptoms of high blood sugar
include frequent urination, increased thirst, and increased hunger. If left untreated, diabetes can
Hyperglycemia or high blood sugar is an abnormally high blood glucose (blood sugar)
level in the blood. Hyperglycemia is a hallmark sign of diabetes (both type 1 diabetes and type 2
diabetes) and prediabetes. The main symptoms of hyperglycemia are increased thirst and a
Angiosperms are vascular seed plants in which the ovule (egg) is fertilized and develops
into a seed in an enclosed hollow ovary. The ovary itself is usually enclosed in a flower, that part
of the angiospermous plant that contains the male or female reproductive organs or both.
does not conform to the International Code of Nomenclature for algae, fungi, and plants.
The circumscription of a subclass will vary with the taxonomic system being used. The only
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
used by some researchers to avoid the confusion that recently surrounds the name
have proposed, "Magnoliidae" as the name used for the entire group of flowering plants, and the
formal name "Magnolianae" for the group of four orders are discussed here.
Clade a group of organisms believed to have evolved from a common ancestor, according
Eczema is a condition that makes your skin red and itchy. It's common in children but can occur
at any age. Atopic dermatitis is long lasting (chronic) and tends to flare periodically. It may be
Emetic a medicine or other substance that causes vomiting. It is something that is given to
Poultice a soft, moist mass of material, typically of plant material or flour, applied to the body to
Beaker refers to a simple container for stirring, mixing and heating liquids commonly used in
many laboratories. Beakers are generally cylindrical in shape, with a flat bottom.
Test tubes refers to a culture tube or sample tube, is a common piece of laboratory glassware
consisting of a finger-like length of glass or clear plastic tubing, open at the top, usually with a
CHAPTER II
A. GUYABANO LEAVES
Kingdom : Plantae
Clade : Plantae
Clade : Angiosperms
Order : Magnoliids
Family : Annonaceae
Genus : Annona
Species : A.muricata
General Features
Guyabano or Soursop Fruit Nutrition Guyabano belongs to the family of Annonaceae, (A.
Distribution
Annona muricata is tolerant of poor soil and prefers lowland areas between the altitudes of 0
metres (0 ft) to 1,200 metres (3,900 ft). It cannot stand frost. The exact origin is unknown; it
Cultivation
The plant is grown for its 20–30 cm (7.9–11.8 in) long, prickly, green fruit, which can have
the Junglesop. Away from its native area, some limited production occurs as far north as
local consumption. It is also grown in parts of Southeast Asia and is abundant on the Island
of Mauritius.
Nutritional Value
The flesh of the fruit consist of a white edible pulp that is high in carbohydrates and
considerable amounts of Vitamin C, Vitamin B1, Vitamin B2, Potassium and dietary fiber.
Medical Uses
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Medicinal Uses of Guyabano has been used as folkloric herbal medicine in many regions
The crushed fresh leaves are also applied on skin eruptions for faster healing. A poultice of
young guyabano leaves is applied on the skin to alleviate rheumatism and other skin
infections like eczema. Applied during the healing of wounds, this can result in less or no
skin scars.
The juice of the fruit is taken orally as a herbal remedy for urethritis, haematuria and liver
Culinary Uses
Aside from being eaten raw, the guyabano fruit is processed into candies, tarts, shakes, ice-
Description
(glucose) levels that result from defects in insulin secretion, or its action, or both. Diabetes
mellitus, commonly referred to as diabetes (as it will be in this article) was first identified as
a disease associated with "sweet urine," and excessive muscle loss in the ancient world.
Elevated levels of blood glucose (hyperglycemia) lead to spillage of glucose into the urine,
Diabetes is a chronic condition associated with abnormally high levels of sugar (glucose) in
the blood. Insulin produced by the pancreas lowers blood glucose. Absence or insufficient
Causes
We can have a diabetes if our blood gets too much sugar. It occurs when your immune
system, the body’s system for fighting infection, attacks and destroys the insulin-producing
CHAPTER III
Methodology
This chapter presents the research design and methodology, including the materials and
Guyabano Leaves. It refers to the main ingredient that is going to use in the study.
Casserole. It refers to the container for the Guyabano leaves (Annona Muricata) in boiling
process.
Knife. It is used to cut the Guyabano leaves (Annona Muricata) from the Guyabano tree.
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Plastic Cylinder. It refers to the containers which contain the Guyabano leaves (Annona
Muricata) extract.
Test tubes. It refers to a culture tube or sample tube, is a common piece of laboratory glassware
consisting of a finger-like length of glass or clear plastic tubing, open at the top, usually with a
Beaker. It refers to a simple container for stirring, mixing and heating liquids commonly used in
many laboratories. Beakers are generally cylindrical in shape, with a flat bottom.
Ladle. It is used to stir the Guyabano leaves (Annona Muricata) from boiling.
Gathering of Materials.
The materials Guyabano Leaves (Annona Muricata), Stove, Casserole, Knife, Plastic Cylinder,
The other materials are gathered from the house of the researchers.
Preparation of Treatments
The research study used 3 treatments. Treatments 0, 1, 2, and it was test through the alternative
Procedure
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Collect Guyabano Leaves from the backyard. Wash the Guyabano leaves with tap water. Get the
casserole and put tap water on it for the boiling process. And put the casserole that filled with
water in the stove. Then, put the washed Guyabano leaves. And get the other materials for
boiling. Boil it until you observe that the water became brownish.
Pre-treatment Evaluation
Identify the level of blood sugar before the treatment. The researchers shall record or test the
0 0 220
1 30ml 220
2 25ml 220
3 20ml 220
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Application of Treatment
The application of the treatments will be held in the ACNHS Laboratory. The rats will receive
each treatment daily for one week. Treatment 0 will have 0% of guyabano leaves extract and 0%
of artificial sweetener. Treatment 1 will have 75% of guyabano leaves extract and 25% of
artificial sweetener. Treatment 2 will have 50% of guyabano leaves extract and 50% of artificial
sweetener. Treatment 3 will have 25% of guyabano leaves extract and 75% of artificial
sweetener.
Post-treatment evaluation
After 3 trials, the blood sugar shall test again if it is reduced by the guyabano leaves (Annona
Muricata) syrup. And if the guyabano leaves (Annona Muricata) syrup has an effect to the high
blood sugar.
The effectiveness of the syrup will be determined, the data were recorded, tabulated, and
Treatment 1 (T1) 1
extract 4
Treatment 2 (T2) 1
Treatment 3 (T3) 1
Table 2. Mean Percentage of Survival Rate of Anti- Dengue capsule treated with varied
concentrations of Dragon fruit peeling and Bamboo leaves extract after 1 week-days observation
period.
SURVIVAL RATE
of survival rate
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Treatment 1
Treatment 2
Statistical Design
Based on the central composite design, two quadratic models were developed to correlate the
preparation variables to the two responses. From the analysis of variance (ANOVA), the most
Workflow of Gathering
Exposure of Treatments
Application of Treatments
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Data Log
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Apendix A
Data Book
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect
Activities Days
Choosing a Topic
Problem Hypothesis
Preparation of Treatments
Documentation
Apendix B
Materials
Efficacy of Guyabano Leaves Extract (Annona Muricata) As a Syrup to Reduce Hyperglycemic Effect