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Int. J. Oral Maxillofac. Surg.

2015; 44: 1131–1137


http://dx.doi.org/10.1016/j.ijom.2015.04.009, available online at http://www.sciencedirect.com

Clinical Paper
Dental Implants

The effect of conventional N. Altiparmak1, S. S. Soydan1,


S. Uckan2
1
Department of Oral and Maxillofacial

surgery and piezoelectric Surgery, Baskent University, Ankara, Turkey;


2
Department of Oral and Maxillofacial
Surgery, Istanbul Medipol University, Istanbul,
Turkey

surgery bone harvesting


techniques on the donor site
morbidity of the mandibular
ramus and symphysis
N. Altiparmak, S.S. Soydan, S. Uckan: The effect of conventional surgery and
piezoelectric surgery bone harvesting techniques on the donor site morbidity of the
mandibular ramus and symphysis. Int. J. Oral Maxillofac. Surg. 2015; 44: 1131–1137.
# 2015 International Association of Oral and Maxillofacial Surgeons. Published by
Elsevier Ltd. All rights reserved.

Abstract. The aim of this study was to evaluate the morbidity following bone harvesting
at two different intraoral donor sites, mandibular symphysis and ramus, and to
determine the effects of piezoelectric and conventional surgical graft harvesting
techniques on donor site morbidity. Intraoral block bone grafts were harvested from
the symphysis (n = 44) and ramus (n = 31). The two donor site groups were divided
into two subgroups according to the surgical graft harvesting method used
(conventional or piezoelectric surgery). Intraoperative and postoperative pain was
assessed using a visual analogue scale (VAS). Donor site morbidity and the harvesting
techniques were compared statistically. Of 290 teeth evaluated in the symphysis
group, four needed root canal treatment after surgery. The incidence of transient
paresthesia in the mucosa was significantly higher in the symphysis group than in the
ramus group (P = 0.004). In the symphysis group, the incidence of temporary skin and
mucosa paresthesia was lower in the piezoelectric surgery subgroup than in the
conventional surgery subgroup (P = 0.006 and P = 0.001, respectively). No
permanent anaesthesia of any region of the skin was reported in either donor site
Key words: bone block grafts; bone augmenta-
group. VAS scores did not differ between the ramus and symphysis harvesting groups,
tion; piezoelectric surgery; autologous bone
or between the piezoelectric and conventional surgery subgroups. When the grafts; morbidity.
symphysis was chosen as the donor site, minor sensory disturbances of the mucosa and
teeth were recorded. The use of piezoelectric surgery during intraoral harvesting of Accepted for publication 21 April 2015
bone blocks, especially from the symphysis, can reduce these complications. Available online 5 June 2015

0901-5027/0901131 + 07 # 2015 International Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
1132 Altiparmak et al.

Bone grafting techniques for pre-prosthet- technique that reduces the risk of damage flap was reflected, and bone defects were
ic alveolar reconstruction, with varying to the surrounding soft tissues and impor- revealed. Next, the donor sites were ex-
degrees of success, are well documented tant structures, such as nerves, vessels, and posed surgically, and a block bone graft
in the literature.1,2 A variety of allografts mucosa, and produces less collateral tissue was obtained by means of a conventional
and alloplastic grafts have been used, damage, resulting in better healing.25,26 bone harvesting procedure or using piezo-
however the vast majority of authors have This surgery also reduces damage to electric surgical equipment (VarioSurg
reported the superiority of autogenous osteocytes during the bone harvesting pro- 50/60 Hz; NSK, Nakanishi Inc., Kanuma,
bone for alveolar reconstruction.3 Autog- cedure. Furthermore, piezoelectric sur- Tochigi, Japan).
enous bone is considered the gold standard gery minimizes patient psychological To harvest the bone graft from the
for osseous reconstruction because it does stress and fear during osteotomy under mandibular ramus, the mucosal incision
not produce immunological reactions and local anaesthesia. It is believed that the was performed medial to the external
contains osteoinductive components.1–4 use of piezoelectric surgery for autoge- oblique ridge and extended anteriorly to
Although the iliac crest is most often nous alveolar bone harvesting may reduce the buccal sulcus of the molar teeth and
used in jaw reconstruction, there is a sig- the risk of sensory disturbance and other posteriorly to the retromolar region. A
nificant risk of resorption with iliac block donor site morbidities.11 mucoperiosteal flap was reflected, expos-
bone grafts.5,6 This disadvantage, and A few reports in the literature describ- ing the lateral aspect of the ramus and the
the fact that dental implants do not ing long-term follow-up studies, claim third molar area. An anterior vertical bone
require a large amount of bone, has led that the mandibular ramus and the retro- cut was performed (the length of the bone
to the increasing use of autogenous block molar areas are associated with less donor block depended on the size of the bone
bone grafts from intraoral sources, partic- site morbidity than the parasymphyseal graft required), and a posterior vertical
ularly from the mandibular symphysis and region.12 However, there is still a lack bone cut was made on the lateral aspect
ramus.7 of information regarding any differences of the ramus. Following the superior hori-
In the repair of localized alveolar in donor site morbidity between the man- zontal bone cut, an inferior horizontal
defects, bone grafts from the symphysis dibular ramus and the symphysis, espe- bone cut was performed to avoid an unfa-
and the ramus offer several benefits: (1) cially when early postoperative results are vourable fracture. Osteotomy lines were
conventional surgical access; (2) the prox- considered. The aims of this study were as elevated with a thin chisel along the entire
imity of donor and recipient sites reduces follows: (1) to evaluate and compare the length of the osteotomy, and the bone
the length of the operation and the time morbidity of the mandibular symphysis block was separated gently to avoid dis-
spent under anaesthesia, making intraoral and ramus donor sites; and (2) to compare turbance of the inferior alveolar nerve.
bone grafts ideal for outpatient implant the effects of different bone harvesting To harvest the bone graft from the
surgery; and (3) minimal discomfort methods (conventional or piezoelectric symphysis, a full thickness horizontal mu-
reported by patients because of the lack surgery) on donor site morbidity. cosal incision was performed in the ves-
of a cutaneous scar and less morbidity as tibular area of the lower incisor teeth from
compared with extraoral locations.1,8,9 the right canine to the left canine, and a
Materials and methods
Despite these advantages, several compli- mucoperiosteal flap was created. The
cations are mentioned in the literature This prospective study was conducted on superior horizontal bone cut was per-
related to bone graft harvesting from the patients undergoing an onlay bone grafting formed at least 5 mm below the apices
symphysis and the ramus. These include procedure, performed by the same surgeon of the incisor teeth and the inferior hori-
tooth numbness, neurosensory distur- in the Department of Oral and Maxillofacial zontal bone cut was performed at least
bances, alterations of mucosa and skin Surgery of Baskent University, between 5 mm above the lower border of the man-
sensitivity, postoperative discomfort (lim- January 2011 and January 2013. Intraoral dible. The size of the bone graft was
ited mouth opening, bleeding, swelling, grafts were harvested consecutively from determined by considering the size of
and pain), and aesthetic problems the mandibular ramus or symphysis; the bone defect at the recipient site. The
(changes in the contour of the donor area harvesting procedures were performed con- vertical osseous cuts were made under
or soft tissue recession).10–22 secutively by either conventional or piezo- copious irrigation with saline. After re-
An advantage of the ramus donor site electric surgery. Patients who were able to moving the corticocancellous bone block
over the symphysis site is fewer com- attend the clinic for follow-up appointments with a bone chisel, additional bone was
plaints of postoperative discomfort. The at 1 and 6 months postoperative were in- harvested from the caudal site with gauges
disadvantages of the ramus area include cluded in the study. and curettes. Sharp osseous edges and
the limited surgical access, limited graft The following patients were excluded: irregularities were reduced to minimize
volume, and potential hazard of damage to those who smoked, patients on medica- postoperative discomfort. A gelatin
the mandibular neurovascular bundle.10 tion, patients with periodontal disease, sponge was applied as a haemostatic
Although these features appear to indicate patients with any systemic disorder, such dressing to the donor area.
the mandibular symphysis as the graft as hypertension, diabetes, rheumatic dis- The bone blocks were all monocortical
donor site of choice,16,23,24 both mandib- ease, or a neurological disease, and bone grafts. The lingual cortex was left
ular symphysis and ramus bone grafts are patients who required revision for an onlay intact at the donor site. The block bone
commonly preferred in clinical practice. bone grafting procedure. graft was adapted and immediately posi-
The use of ultrasonic vibrations to cut tioned over the recipient area. This graft
bone was first introduced two decades ago was fixed to the recipient site with two
Surgical procedures
to overcome the limitations of traditional titanium miniscrews that were 1.5 mm wide
instrumentation in oral bone surgery; this The recipient area was first prepared. Mid- and 10 mm long (Synthes GmbH, Oberdorf,
was done by modifying and improving crestal and vertical mucosal incisions were Switzerland), and were removed during
conventional ultrasound technology. Pie- made along the recipient area, depending implant placement. Any sharp angles of
zoelectric surgery is a minimally invasive on the graft localization. A mucoperiosteal the bone block that could perforate the
Donor site morbidity of symphysis and ramus 1133

overlying mucosa were removed with a skin was also assessed by two-point dis- Inc., Chicago, IL, USA). Ordinal data
round bur. The periosteum of the buccal crimination test. Patients were asked to were recorded as the median (mini-
flap at the recipient site was incised to allow differentiate the number of contacts on the mum–maximum); the number of cases
the adaptation of the wound margins to be as mucosa and skin with their eyes closed. and percentage were recorded for nominal
tension-free as possible. Both the donor and The results were classified into three data. The Wilcoxon signed rank test was
the recipient sites were sutured with resorb- groups: distance <7 mm indicating no used to determine whether differences in
able sutures, and the sutures were removed alteration, distance 7–11 mm indicating median VAS scores between groups were
1 week later. a slight alteration, and distance >11 mm statistically significant. Nominal data
indicating impaired sensitivity. were analyzed using Pearson’s x2, conti-
Assessments The vitality of the teeth adjacent to the nuity corrected x2, or Fisher’s exact test,
harvesting sites was evaluated using an as applicable. A P-value of less than 0.05
Clinical symptoms such as excessive in- electric pulp test (Pulp Vitality Tester 9V; was considered statistically significant.
traoperative bleeding and a prolonged pe- Parkell Inc., Edgewood, NY, USA) and For all possible multiple comparisons,
riod of healing at the donor site were the necessity for root canal treatment was the Bonferroni correction was applied to
recorded for all graft harvesting proce- evaluated at 6 months postoperative. control type I error.
dures. A visual analogue scale (VAS) The symphysis (n = 44) and ramus
was used to assess both intraoperative (n = 31) groups were divided into sub-
and postoperative pain. Results
groups according to the surgical graft
Parameters of sensory disturbance were harvesting method used: conventional sur- Out of 105 consecutive patients, 64 (16
evaluated by the same clinician (NA) on gery or piezoelectric surgery. First, the males and 48 females) were included in
both the oral mucosa and adjacent skin parameters for donor site morbidity of this study after applying the inclusion and
(Fig. 1) at 1 and 6 months postoperative. the ramus and symphysis groups were exclusion criteria. These 64 patients un-
The superficial sensory function of the oral compared, and then the subgroups of each derwent 75 bone grafting operations. They
mucosa and adjacent skin was assessed by group were compared. ranged in age from 17 to 71 years (mean
means of the pointed–blunt test (cotton age 44.8 years).
pellets were used to lightly touch the Forty-four bone harvests were from the
Statistical analysis
mucosa and skin for the evaluation of mandibular symphysis (symphysis group)
tactile sensitivity). The superficial sensory The data analysis was performed using and 31 were from the mandibular ramus
function of the oral mucosa and adjacent SPSS for Windows version 11.5 (SPSS (ramus group). In the symphysis group, 13
bone harvests were done by conventional
surgery and 31 by piezoelectric surgery. In
the ramus group, 15 bone harvests were
done by conventional surgery and 16 by
piezoelectric surgery.
No sign of major sensory disturbance,
permanent paresthesia (anaesthesia), hy-
peresthesia, or hypoesthesia of the oral
mucosa or the adjacent skin was detected
in either the symphysis group or the ramus
group at 6 months postoperative. Howev-
er, different incidences of minor sensory
disturbance (temporary paresthesia) were
observed in the symphysis and ramus
groups at 1 month postoperative.
There was no statistical difference be-
tween the symphysis and ramus groups
with respect to the incidences of excessive
intraoperative bleeding and a prolonged
healing period. Excessive intraoperative
bleeding was seen in three cases in the
ramus group (9.7%), whereas it was not
seen in any case in the symphysis group. A
prolonged healing period was detected in
five cases in the ramus group (16.1%) and
in four cases in the symphysis group
(9.1%).
When temporary paresthesia of the skin
was assessed in the patients in the ramus
and symphysis groups by pointed–blunt
test, no statistically significant difference
was found between the two groups. How-
Fig. 1. (A) The region of oral mucosa evaluated for the symphysis group; (B) the region of oral ever, temporary paresthesia of the oral mu-
mucosa evaluated for the ramus group; (C) the region of skin evaluated for the symphysis group; cosa, as assessed by the pointed–blunt test,
(D) the region of skin evaluated for the ramus group. was significantly higher in the symphysis
1134 Altiparmak et al.

Table 1. Results of the pointed–blunt test for the ramus and symphysis groups. pointed–blunt test results, there was a
Parameters Ramus group (n = 31) Symphysis group (n = 44) P-valuea statistically significant difference in the
incidence of temporary paresthesia
Skin 1.000b
Normal 27 (87.1%) 38 (86.4%) between the two bone graft harvesting
Temporary paresthesia 4 (12.9%) 6 (13.6%) subgroups (conventional surgery and pie-
Mucosa 0.004c zoelectric surgery) of the symphysis group
Normal 28 (90.3%) 25 (56.8%) when the results of the two-point discrim-
Temporary paresthesia 3 (9.7%) 19 (43.2%) ination test of the oral mucosa and the skin
a
According to the Bonferroni correction, P < 0.025 was considered statistically significant. were evaluated: temporary paresthesia of
b
Fisher’s exact test. both the skin and the oral mucosa was
c
Continuity corrected x2 test. significantly lower in those patients in the
symphysis group who underwent piezo-
electric surgery (Table 5).
Table 2. Results of the two-point discrimination test for the ramus and symphysis groups. There was no statistically significant
Parameters Ramus group (n = 31) Symphysis group (n = 44) P-valuea difference between the two bone graft
harvesting techniques (conventional sur-
Skin 0.039b
<7 mm 31 (100.0%) 38 (86.4%) gery and piezoelectric surgery) used in the
7–11 mm 0 (0.0%) 6 (13.6%) ramus group when intraoperative and
Mucosa 0.142c postoperative median VAS pain scores
<7 mm 26 (83.9%) 29 (65.9%) were considered (1.8 vs. 1.9). However,
7–11 mm 5 (16.1%) 15 (34.1%) the median intraoperative VAS score of
a
According to the Bonferroni correction, P < 0.025 was considered statistically significant. the symphysis group was higher with con-
b
Fisher’s exact test. ventional surgery (3.1) than with piezo-
c
Continuity corrected x2 test. electric surgery (1.6) (P < 0.001). There
was no statistically significant difference
between the two bone graft harvesting
group (P = 0.004) (Table 1). When tempo- There was no statistically significant techniques in the symphysis group when
rary paresthesia of the skin and mucosa difference in the incidence of temporary the postoperative median VAS pain scores
was assessed in patients in the ramus and paresthesia in the ramus group according were considered (Table 6).
symphysis groups by two-point discrimina- to the two bone graft harvesting techni- In the ramus group, 32 teeth were eval-
tion test, there was no statistically signifi- ques used (conventional surgery and pie- uated in the conventional surgery sub-
cant difference between the two groups zoelectric surgery), when the results of the group and 28 teeth in the piezoelectric
(Table 2). pointed–blunt test of the oral mucosa and surgery subgroup. In the symphysis group,
There was no statistically significant the skin were evaluated. However, there 113 teeth were evaluated in the conven-
difference between the symphysis and was a statistically significant difference in tional surgery subgroup and 177 in the
ramus groups for intraoperative and post- the incidence of temporary paresthesia in piezoelectric surgery subgroup. There
operative VAS scores, the necessity for the symphysis group according to the two was no statistically significant difference
root canal treatment to the adjacent tooth, bone graft harvesting techniques used between the two bone graft harvesting
or for a negative vitality test result. The (conventional surgery and piezoelectric techniques in the ramus group when the
intraoperative and postoperative median surgery), when the results of the point- necessity for root canal treatment and the
VAS pain scores were similar in the ramus ed–blunt test of the oral mucosa and the loss of vitality of adjacent teeth were
and symphysis groups (Table 3). Sixty skin were evaluated: temporary paresthe- considered. In the symphysis group, the
teeth related to the bone graft harvesting sia of both the skin and the oral mucosa loss of vitality of adjacent teeth was sig-
site were evaluated in the ramus group and was significantly lower in those who un- nificantly lower in the piezoelectric sur-
290 teeth were evaluated in the symphysis derwent piezoelectric surgery (Table 4). gery group than in the conventional
group. Root canal treatment was not re- The results of the two-point discrimina- surgery group. The necessity for root canal
quired in the ramus group; in the symphy- tion test of the oral mucosa and the skin treatment (n = 4) was observed only in the
sis group, however, four root canal were similar for the conventional surgery conventional surgery subgroup of the sym-
treatments of an adjacent tooth were need- and piezoelectric surgery subgroups in the physis group. The loss of vitality of adja-
ed (Table 3). ramus group. In concordance with the cent teeth was higher with conventional

Table 3. Comparison of the intraoperative and postoperative VAS pain scores, the necessity for root canal treatment, and the incidence of loss of
vitality of the tooth adjacent to the bone harvest site in the ramus and symphysis groups.
Parameters Ramus group (n = 31) Symphysis group (n = 44) P-valuea
Intraoperative VAS score, median (range) 1.9 (0.0–6.1) 1.9 (0.0–5.0) 0.500b
Postoperative VAS score, median (range) 1.3 (0.0–4.0) 1.5 (0.0–5.8) 0.862b
Necessity for root canal treatment 0 (0.0%) 4 (1.4%) 1.000c
Negative vitality test result 8 (13.3%) 40 (13.8%) 1.000d
VAS, visual analogue scale.
a
According to the Bonferroni correction, P < 0.025 was considered statistically significant.
b
Mann–Whitney U-test.
c
Fisher’s exact test.
d
Continuity corrected x2 test.
Donor site morbidity of symphysis and ramus 1135

Table 4. Results of the pointed–blunt test for the ramus and symphysis groups according to the surgery in both the ramus and the symphy-
bone harvesting technique. sis groups, although this was not significant
Conventional Piezoelectric for the ramus group (Table 6).
Parameters surgery surgery P-valuea
Ramus group Discussion
Skin 0.043b
Normal 11 (73.3%) 16 (100.0%) Many studies on intraoral alveolar bone
Temporary paresthesia 4 (26.7%) 0 (0.0%) harvesting have focused mainly on the
Mucosa 0.600b reconstructive procedure at the recipient
Normal 13 (86.7%) 15 (93.8%) site,9,27–30 or on the complications related
Temporary paresthesia 2 (13.3%) 1 (6.3%) to harvesting. Only a very limited number
Symphysis group
Skin 0.006b
of studies have reported the results of the
Normal 8 (61.5%) 30 (96.8%) harvesting procedure,30 and all have con-
Temporary paresthesia 5 (38.5%) 1 (3.2%) firmed the superiority of the ramus over
Mucosa 0.001c the symphysis as an intraoral donor site,
Normal 2 (15.4%) 23 (74.2%) since it has less associated morbidity. In
Temporary paresthesia 11 (84.6%) 8 (25.8%) contrast to the common use of mandibular
a
According to the Bonferroni correction, P < 0.0125 was considered statistically significant. bone grafts, data related to the comparison
b
Fisher’s exact test. of mandibular ramus and symphysis donor
c
Continuity corrected x2 test. site morbidity are still sparse. For this
reason, the present study was conducted
to compare the donor site morbidity result-
ing from the harvesting of bone from the
Table 5. Results of the two-point discrimination test for the ramus and symphysis groups mandibular symphysis and the ramus.
according to the bone harvesting technique. Cordaro et al.12 reported significantly
Conventional Piezoelectric higher paresthesia of the oral mucosa ad-
Parameters surgery surgery P-valuea jacent to the mandibular symphysis bone
Ramus group harvesting site than the mandibular ramus
Skin – site as assessed with the pointed–blunt
< 7 mm 15 (100.0%) 16 (100.0%) test, similar to the present results. Howev-
7–11 mm 0 (0.0%) 0 (0.0%) er, a markedly lower percentage of tem-
Mucosa 1.000 porary paresthesia of the oral mucosa
< 7 mm 13 (86.7%) 13 (81.3%) adjacent to the mandibular symphysis
7–11 mm 2 (13.3%) 3 (18.7%) bone harvesting site was reported in the
Symphysis group
Skin 0.006
study of Cordaro et al.,12 compared with
< 7 mm 8 (61.5%) 30 (96.8%) the first month result in the present study
7–11 mm 5 (38.5%) 1 (3.2%) (5.4% vs. 43.2%). A possible reason for
Mucosa <0.001 this difference may be the long-term fol-
< 7 mm 2 (15.4%) 27 (87.1%) low-up (mean 29 months) of the study by
7–11 mm 11 (84.6%) 4 (12.9%) Cordaro et al.12
a
According to the Bonferroni correction, P < 0.0125 was considered statistically significant; Recent studies have reported that
Fisher’s exact test. both ramus and symphysis harvesting
procedures are well accepted by patients,
but that the ramus procedure is generally
preferred.30 The harvesting and grafting

Table 6. Comparison of the intraoperative and postoperative VAS pain scores, the necessity for root canal treatment, and the incidence of loss of
vitality of the tooth adjacent to the bone harvest site in the ramus and symphysis groups according to the bone graft harvesting technique.
Parameters Conventional surgery Piezoelectric surgery P-valuea
Ramus group
Intraoperative VAS score, median (range) 1.8 (0.0–6.1) 1.9 (0.0–5.8) 0.446b
Postoperative VAS score, median (range) 1.8 (0.0–4.0) 1.1 (0.0–4.0) 0.922b
Necessity for root canal treatment 0 (0.0%) 0 (0.0%) –
Negative vitality test result 7 (21.9%) 1 (3.6%) 0.057c
Symphysis group
Intraoperative VAS score, median (range) 3.1 (1.8–4.3) 1.6 (0.0–5.0) <0.001b
Postoperative VAS score, median (range) 1.8 (1.3–2.5) 1.0 (0.0–5.8) 0.051b
Necessity for root canal treatment 4 (3.5%) 0 (0.0%) 0.022c
Negative vitality test result 28 (24.8%) 12 (6.8%) 0.001d
VAS, visual analogue scale.
a
According to the Bonferroni correction, P < 0.0125 was considered statistically significant.
b
Mann–Whitney U-test.
c
Fisher’s exact test.
d
Pearson’s x2.
1136 Altiparmak et al.

procedures were most often performed in Competing interests harvesting intraoral block bone graft. J Pharm
the same surgical field in the ramus group Bioallied Sci 2012;4(Suppl. 2):S165–8.
None declared. 11. Misch CM. Comparison of intraoral donor
in the present study; therefore, it may have
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ate the discomfort caused by each of these placement. Int J Oral Maxillofac Implants
Ethical approval
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study, there was no significant difference tional Review Board and Ethics Commit- resanto MV, Eliopoulos D. Mandibular bone
tee of Baskent University (Project No. D- harvesting for alveolar reconstruction and
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bleeding were considered. Excessive in- Res 2011;22:1320–6.
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and clinical indications of piezoelectric bone device to harvest bone grafts from the man- 11
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collagen membranes: a clinical study with 42 bone graft. Presentation. Second Balkan Tel: +90 3122151336; Fax: +90 3122152962
patients. Clin Oral Implants Res 2006;17: Congress of the Balkan Association of Max- E-mail: nuraltiparmak@hotmail.com
359–66. illofacial Surgeons (BAMFS) and 5th Oral

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