KOREAN J. FOOD SCI. TECHNOL. Vol. 16, No. 3 (1984) Of AAAe| RSAAD I ABALALS| ofols-at ZA wae aSe eeawled sgedae4 Optimum Conditions for Extracting Alginic Acid from Undaria Pinnatifida and Amino Acid Composition of Its Extraction Residue Kil-Hwan Kim and Jong-Joo Cheong Food Science and Technology Laboratory, Korea Advanced Institute of Science and Technology, Seoul Abstract ‘The optimum conditions for extracting alginic acid from the powdered Undavia pinnatifida and amino acid composition ofits extraction residue were investigated. Extraction with 60 volumes of 1.0% sociium carbonate solution to the sample at 80°C gave a maximum yield of alginic acid and the optimum extraction time was 3 hours when all the other extraction conditions had been satisfied. In the process of precipitating alginic acid gel from algin solution, the highest yield was obtained at pH 2.0 and 1.0% H,SO, was more suitable than 10% HCI as a precipitating agent. Extraction residue remained by separating algin solution contained 51.5% (on the dry baiss) of crude protein, and its limiting amino acid and protein score were lysine and 41.5, respectively. M2 e124 (Undaria pinnatifida) & $3 ob lstot AME 22824 Feldetoldes G2 Aal aae al ireb~soreol aaisia ake aed ala viata wee ade Age delet ee a guleiog ABAe| ABSA Wool olsbtol zwbbwel Lee ol ote} elder Saat de daleed Rea LF MES|a| Zoha Veo] seo] Sr asegs Ageia Qe, ayes eagle] ¥4ab Feley Aol vAo} HF af ofefel of Sys1S abehale|7} 9} Gate Wy 20 Wee Gail, dasa S22A4 LL FE Hobslsol SYtoe ofa 4 ako!” aa) sfo} geo}, aalelol 9) ARH Aells| Fag Bel ATE Fe AE Avs Cad Le} Fasiel eh, elvtel 4S] EAE. vleagye” so) ABupgol walsh wh alt, ela} a ol k 284 vledaed af Hotta} 336 H. QAALS Aebol cabzladol alee ol zabg 4 elt Fell HE SSPE MU oes A alae ae.” aa cerideS| BAS BEAU oh, Fulsl Ualdbel Be tse easel ee aL ofa] alata estab qhalabel 4 BAL OAS ade) obebol Bal Pz told eas Pole dal Baupyerst 4 gan zala Qala Hal Agel) BEd B tae sel orl yopmel4t GL Ecklonia sh he alot] AA Eisenia bicylis®) VAF%| 2 Aol) BU UENO ae, olwel REM GoldE FE Bad AMS obstate Sdol Bel der seal, BAG Woe dale Ta ued maxima?) als 222o] BE LHVol. 16, No. 3 (1984) Assam das) wel $4" aaa qaladclobal (Alginate lyase)ol BE AFM de Vags] 24 Sago] AY APES EF eh clea ATES BAA AAS) AAO Be LFS ARO alae S 4 Aah UE ATEA oletel dade) Bel dt 24 lsh Galtdol Bel APE aol B+ aie. Ha} & APOE Ie FEY Aloe yet HEALS olBs}7] AY AES oHRLaIA1 alalel, # Avid dade Fe2de Asse dad Fou AES Selle FOU colina ade e aaa. AE SY Be ME ABB AS 19638 124 gol Aol Fe EM A1GLSA hammer mille A-F5}2} 60 mesh 2 AD EARS AR Eetelets ye gat SL Gale UHL Table 15} 2d, aaltel Se AR ZY/S-E SOC ~GHOT 2 0.04% NaOH $474, AEA 0.5% HCLSetol AE Ladd ale # ol stakatsh. olold 2~351 al, ofsteh # Na, CO. $28 of Aalshe UM mised dae Re eeto. FSA HAL 8, COOrpmol4 20¥2b Wile ol a ASAT HE Helalstet, lol dade Aad A12\ 18h} 10% HS0.9} 10% HCE At YrHsIe} cle pHs Gadel FH Sasiate, deal V4 ALAS AES] BO%methanol-$ 7+8ho4 Waring blendor = baste) LELE AAG F 15T ovenshd SE eo] 2 yal Aaa ol¥ Pestadeor} ceajoy o] the +H= Albstsict. BAAS RB4H(%) Bad ALSG) asl 2EEGG) “ UA ABTS! ofolias ZH ANYEL AOAC Mol Zeigleorl, cole 24 Table 1. Proximate composition of the powdered Undaria pinnatifida Moisture (%) BS Crude protein (%) 1.3 Crude fat (%) L4 Ash 30.9 Crude fiber (%) 3.6 Crude sugar (%) 4.3 Allginie acid (%) 4.2 sled galas Sears cboletad 337 & amino acid autoanalyzer (Beckman model 116) 2 #4 seal tryptophan Koblers'" 2) eel at of ARS 7teeal abetz cystine® More'™ 2] Y ot leo} cysteic acided Yat # half-eystine 28 BAsaleo} pola) obelsabe FQ aleh stslol Aas rewbalsted eA abetct Bae De oot aie! Sezz NuCOsl $5 YAEL HE oll dado F #H2 Fig. Lot Ue, lst NaC Be ARE Fo] COMB (w/v) S AFAR ASAAL 341k ol Bor ASS 10% H-SO.F 7Hle] pHE 202 2 asl delslet, astl debe visidel +e-¢574 OT late ae BAA Sel olteyala — Bet NesCO.8] $501 atalel Valalsl RB4Eol 16% olsen} a0 ol 4olle NaCOrss7t 1.0% Yet Fl 2.0624 EE FEE Vat, GT of aol) LE FES Vel PE Zar} See ae sla] Zebol Rggebol olefa] wp aaee alate] =18 x =. 14 5 «+ 90°C = 400 pata © 60°C —e 40°C ++ 25°C 0 05 10 15 20 Concentration of Na,CO4%) 1. Effect of concentration of sodium carbon ate and extraction temperature om the yi- eld of alginic acid from Undaria pinnatifida338 aad ase Adela RAGE Roe Vsiate, £4 NCO, BAT SEH LOGY We FeESO Basle} FETE) 0% MEA YE FEE VRE oe BE Nacleesol Aste} aeesol Mspal Reba deel dee Basis 3 Gade p-1.4-D =mannuronic acid7} B-1, 4 tot eisteh aaa 4 ALS] Label Bol, d-Logluronic acid7h ee F ASLLLA EVA AEslo} AWE hetero polys- accharide ol #2 ¥-bfol carboxyl7i7# Felsto} Sh Absholl Na’, Ca’ $s} olfel Asia yt 2S olFol GF IAS Beal ek aae. 2 B2H AME FZ EEN Ca! SALE NaCOE Azlsol FE4el Neos a BAge2m S80] olfoldd, 2422 NaCO. 2} ES LOA Coolest Nachezts| alabelge) aah ae Zeb) aldol FB4E0] tobe Bes at Slate, ZL NasCOv2} SE 1.5% la le F SF#o See] ots AME Usted ole Ne CO.) SE7 HobAel shel FEAST pH HA He chia) Hee) Ae Ase. Haugé"2 pectin ol dtl BetFold f-climination reaction) sto} He}sto} $2} uronic acid FEMS Babs ol Yield (%) Extraction volume of 1%N (Volume / Sample wt) Fig.2. Effect of extraction volume of 1% Na. C and extration time on the yield of algi acid from Undaria pinnatifida ede at Mita z BALLS. HAE 7latot stata} plo ose od SAS] elt Loy Soha] ast stole dz age aeee ojo gage sea op Bol4S NaCO2) FE Holle tet pH ste seb Ut qaldlol Bekslo} olf ABs al dol oieisl4i7] Heol Fol Ye Aes B2tstal oh, Fig, 2 NaCOBN SSE 1.09, REESE BOTS BYSHD ASA 10% HSOE AAO DHS 2022 BR W OFA] AS SL FHA1E 4 Lo] FEERE 24C Ashe]. NaCO, HAS ARDS 1RSB COMB (w/v) 14 AAR Bt Ada Hel oso 22.0% W919) FEF SS BA 4M oltbolde FH4-He] 20% oO dt BA USkeh, lela ASE NasCOrBol oh ABO} af € de 29] Bee] Urbs Rs] Heel Ae 3 U7 Hib" Eeklonio mazimas} BA Po MY ATE FHT ON NACOS4S AF 2ASDS 4129] 20S AST oH HP se wv teol ALeo) olvet Zoe + Hel seobded) + Uele Fsihda vaselet. eleys] ApoE Ab Seo] soda of FAG FOALE velal ashe 4 Conga sist ISG FES wach ee REA atel dela +H FHFHC Sree 3412b o} Hole 2 Stel FAA YolaA Use YF UW th Aes eto] Bade 1.0% Ne 5) COMBS Bebe} BOL 341 FA ole AGE Wee Brtsiaie. aie tez2 HS BEA Nt HE pastete 24 | stot etal. abe telsiit yepee Se 2 rhalo} AA SBS| MOL sel Uvele F AH BASAL Galas] FHA Nadel Sol elo Abe rbalod qtalol Na‘ olf H' elfap a] ab 4 ESE UA Agel s WA AEA ach Fig. 32 Gate] ASAZA 10% HSOS 10 % HS Bebo} pHs Vat RETES SAF Ala 2 Ab pH2.0F tea aed y- 10% 1.S0.7 10% HCL ASBaIt ol SRO wae. Uebsteh, HaugS'"& Balad Betel pH 5.0 lat AID oe Hele TE Asataat Dold a 4s} deh ealsa4 Gots] aAsol SAA so} Ade ald, aioe lee AeA sel 4) de jagdea sp] We ded dade $4 ALS arpafsn alsboloh OechVol. 16, No. 3 (1984) oo a 10% -H,80, , 10%-HCI 60 40 PH Fig.3. Effect of pH of algin solution on the fo- forming alginic acid gel 2015 dase BY! ze FEE HE VRE BIE aeslE UAFS BA 1S ASAT Gohwrl Astoy Fal AEE EAs Abo] Uae 24 L Tablezol) HELE wbstato) Zabel] SLSWIAEANT) 24% 7 18.2% AO} Ofsted ZalMo| 11.1%, SSHES/ 9.1 % NAH. cetat ass sab] dey at gepol of F ERE E+ seal ol deal oid age = AML AME Table 33} Fd. £4 Table 4= att aetael ME Aes astol Qpebolcabal 2 AG ANE Wee, a Yat dar} 41.584 sine o| Adofojaatel Boe vested vleagel Ase Abgolal o}4}= lysineo] Ao}o| abo ey Ebel Crude fiber (%6) Crude sugar (%) ‘Table 2. Proximate composition of alginic acid extraction residue Moisture (%) 90.1 Crude protein (%) 5.1 Crude fat(%) La Ash(%) 09 18 1.0 ciel dats) Sears bended 339 Table 3. Amine acid composition of alginic acid extraction residue Content ‘Amino acid mg/100g sample dry basis (%) Lysine 139.8 Lal Histidine 97.6 0.99 Arginine 219.7 2.83 Tryptophan 80.9 0.82 Aspartic acid 585.0 5.91 Threonine 262.7 2.65 Serine 252.7 2.55 Glutamic acid 694.9 7.02 Proline 217.9 20 Glycine 318.4 3.19 Alanine 428.5 31 Half cystine 28.2 28 Valine 328.6 32 Methionine 17.5 19 Isoleucine 230.7 3 Leucine 532.5 38 Tyrosine 176.5 8 Phenylalanine 282.2 85 Toul 49.5 51.01 ‘Table 4. Essential amino acid composition of Undarte pinnotfdat fond and pe) and Mine extraction vesdue Undaria pinnatifida’” Alginic Amino aid Reference” scien frond stipe tion residue iiss DC Lye LTA Netoine Ei wo 96 a 8a Cyiie Netione 6.05.3 BL Cyne 08 Peplaine a7.g 2 a8.9 Trine Phalaine 1.4 LSA Tree ei mest tome mn a ce ane ot eeoneeo senn Vale ma 37 A Teme = 9.295 10.0 LS Typos 2.6 5.03.6 Lining enna acid _Iysine aoleveine sine ee as Protein score 4340 VAR ASE eases JHE H.AVSOY AE AFM ALE isoleucine’! MdobleAeca eat7yt 83 solgich seve eel vlatel Gaze a das Evel ade olan ASS E+ Loot Fa Robel nabs) shy al cystine) #Gs aM Broz USS t+ alot fo 0 ofstoldsl bala 2a zz 4 24g Azad. dade aol BBA COMBA 1.0% Ni 49 2842 of OCIA FSH Wel Ha Hol Eatee, + FAVE BAA oldol Aeeletd. Ee Yager A UALALAS ASHE aol Solel pHE 2.02 SSATP Fol Eiken}, old AeA 10% HSOF AE B47h 10% HCI Wwe He 2 FES Us UMELS Hea Fee SUAS ALS LAG Gat aed tol 51.5% (AE FE) ROvi, obvxat ZAoll lela A eobol sab ol lysine 22.4) MH 41 Soh. 1. SRP, ERP Gace: ea Estes, 8.15 1975) 2, HN, SmRHM, thE B44 ets] 213, 120(1970) 3. 148, MES, ola, Sai eadses.s, 371970) 4, ANE ASAPH Agius (Bole AMSA) (I97S) 5. as, Ul: G34 eabetalal, 14, 336 (1982) 6. dae, UA: G4 Boteh ala, 15, 277 (1983) 7. SHAME, AGH, FAC: AFIS a1, 9, 691976) 8. EM, ARAN, IB, fKRR: See 44 (1968) 9. 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