Standard curve for Estimation of Protein(Biuret

Assay
0.6

0.5
Absorbance(nm)

0.4

0.3

0.2

0.1

0
0 1 2 3 4 5 6
Concentration(mg/ml)

Figure 1.1: showing the graph of absorbance to the function of protein concentration.

Figure 1.2: shows the colour of the solutions in the 7 test tubes after they were
incubated.

M=( y 2− y 1) /( x 2−x 1)
M=(0.494-0)/(5-0)
M=0.099

y=mx+c
0,243=0,099(x)+0
X=2.45
That means the concentration of the unknown protein solution is 2,45 mg/ml