Protocol report

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General info

Protocol information
Protocol name ....
Modified by hannah.pezzi
Kit name MagMAX MVPI and MVP II
Description MagMAX MVP for Viral RNA extraction from 400uL Liquid sample

Run information
Execution started 2022-06-27 13:45:10+08:00
Executing user ameer.affandi
Total warnings 0
Total errors 0

Laboratory information
Laboratory name
Address
Phone
E-Mail

Instrument parameters
Instrument name 713-8G0100
Instrument type KingFisher Presto
Firmware version 1.2.3
Instrument serial number 713-8G0100

Software parameters
Software version BindIt Software 4.0 for KingFisher Instruments

Run log
2022-06-27 13:45:10 ....: started
2022-06-27 13:45:10 Temperature: 29 / 33 °C
2022-06-27 13:45:12 Pick-Up: 'Tip1' load.
2022-06-27 13:45:12 Load plate: started
2022-06-27 13:45:16 Load plate: started
2022-06-27 13:45:28 RotateDial: started
2022-06-27 13:45:30 Change plate: started
2022-06-27 13:45:30 Remove plate: started
2022-06-27 13:45:30 Load plate: started
2022-06-27 13:45:30 Extraction0: started
2022-06-27 13:45:30 Change plate: started
2022-06-27 13:45:30 Remove plate: started
2022-06-27 13:45:30 Load plate: started
2022-06-27 13:45:33 Change plate: started
2022-06-27 13:45:33 Remove plate: started
2022-06-27 13:45:33 Load plate: started
2022-06-27 13:45:33 Extraction: started
2022-06-27 13:45:33 Change plate: started
Protocol report
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2022-06-27 13:45:33 Remove plate: started

2022-06-27 13:45:33 Load plate: started
2022-06-27 13:45:40 Temperature: 29 / 33 °C
2022-06-27 13:46:10 Temperature: 28 / 33 °C
2022-06-27 13:46:40 Temperature: 29 / 33 °C
2022-06-27 13:47:10 Temperature: 29 / 33 °C
2022-06-27 13:47:40 Temperature: 29 / 33 °C
2022-06-27 13:48:10 Temperature: 29 / 33 °C
2022-06-27 13:48:40 Temperature: 29 / 33 °C
2022-06-27 13:49:10 Temperature: 29 / 33 °C
2022-06-27 13:49:40 Temperature: 29 / 33 °C
2022-06-27 13:50:10 Temperature: 29 / 33 °C
2022-06-27 13:50:40 Temperature: 29 / 33 °C
2022-06-27 13:51:10 Temperature: 29 / 33 °C
2022-06-27 13:51:40 Temperature: 29 / 33 °C
2022-06-27 13:52:10 Temperature: 29 / 33 °C
2022-06-27 13:52:12 Wash1: started
2022-06-27 13:52:14 Change plate: started
2022-06-27 13:52:14 Remove plate: started
2022-06-27 13:52:14 Load plate: started
2022-06-27 13:52:40 Temperature: 29 / 33 °C
2022-06-27 13:53:10 Temperature: 29 / 33 °C
2022-06-27 13:53:40 Temperature: 30 / 33 °C
2022-06-27 13:54:10 Temperature: 30 / 33 °C
2022-06-27 13:54:40 Temperature: 30 / 33 °C
2022-06-27 13:54:50 Wash2: started
2022-06-27 13:54:52 Change plate: started
2022-06-27 13:54:52 Remove plate: started
2022-06-27 13:54:52 Load plate: started
2022-06-27 13:55:10 Temperature: 30 / 33 °C
2022-06-27 13:55:32 Elution1: started
2022-06-27 13:55:34 Change plate: started
2022-06-27 13:55:34 Remove plate: started
2022-06-27 13:55:34 Load plate: started
2022-06-27 13:55:40 Temperature: 30 / 33 °C
2022-06-27 13:56:10 Temperature: 30 / 33 °C
2022-06-27 13:56:40 Temperature: 30 / 33 °C
2022-06-27 13:57:10 Temperature: 30 / 33 °C
2022-06-27 13:57:40 Temperature: 30 / 33 °C
2022-06-27 13:58:10 Temperature: 30 / 33 °C
2022-06-27 13:58:40 Temperature: 30 / 33 °C
2022-06-27 13:59:10 Temperature: 30 / 33 °C
2022-06-27 13:59:40 Temperature: 30 / 33 °C
2022-06-27 14:00:10 Temperature: 30 / 33 °C
2022-06-27 14:00:40 Temperature: 30 / 33 °C
2022-06-27 14:00:56 Elution2: started
2022-06-27 14:01:10 Temperature: 30 / 33 °C
2022-06-27 14:01:40 Temperature: 30 / 33 °C
2022-06-27 14:02:10 Temperature: 30 / 33 °C
2022-06-27 14:02:40 Temperature: 30 / 33 °C
2022-06-27 14:03:05 Leave: 'Tip1' leave.
2022-06-27 14:03:07 Remove plate: started
2022-06-27 14:03:10 Temperature: 30 / 33 °C
2022-06-27 14:03:22 Remove plate: started
2022-06-27 14:03:24 ....: completed
Protocol report
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Reagent info

Sample 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
Binding/Bead Mix 275 - Reagent
Sample 200 - Reagent
PK/MS2 10 - Reagent

Wash 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
Wash Soln 500 - Reagent

80p_EtOH 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
80% Ethanol 1000 - Reagent

Elution 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
Elution Solution 100 - Reagent

TipComb 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
- - - -

Empty 96 DW plate
Name Well volume [µl] Total reagent volume [µl] Type
NoReagent 50 - Reagent

Dispensed reagents

The protocol does not contain dispensed reagents

Protocol report
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Steps data

Tip1 96 DW tip comb

Pick-Up TipComb

RotateDial Empty
Beginning of step Precollect No
Release beads No
Mixing [none]
Heating during mixing No
End of step Postmix No
Collect beads No

Extraction0 Sample
Beginning of step Precollect No
Release beads No
Mixing [none]
Heating during mixing No
End of step Postmix No
Collect beads No

Extraction Sample
Beginning of step Precollect No
Release beads Yes
Mixing / heating: Mixing time, speed 00:05:00, Fast
Heating temperature [°C] 70
Preheat Yes
End of step Postmix No
Collect count 5
Collect time [s] 0

Wash1 Wash
Beginning of step Precollect No
Release time, speed 00:00:10, Bottom mix
Mixing / heating: Shake 1 time, speed 00:00:10, Bottom mix
Shake 2 time, speed 00:00:10, Fast
Loop count 2
Heating during mixing No
End of step Postmix No
Collect count 5
Collect time [s] 1

Wash2 80p_EtOH
Beginning of step Precollect No
Release beads No
Mixing / heating: Mixing time, speed 00:00:20, Medium
Heating during mixing No
End of step Postmix No
Collect beads No
Protocol report
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Elution1 Elution
Beginning of step Precollect No
Release beads Yes
Mixing / heating: Shake 1 time, speed 00:00:15, Bottom mix
Shake 2 time, speed 00:00:45, Medium
Loop count 5
Heating temperature [°C] 70
Preheat Yes
End of step Postmix No
Collect beads No

Elution2 Elution
Beginning of step Precollect Yes
Release beads No
Mixing / heating: Mixing time, speed 00:01:00, Slow
Heating during mixing No
End of step Postmix No
Collect count 2
Collect time [s] 15

Leave TipComb