Effect of Formaldehyde and Methanol mixture against feed grain

pathogen Salmonella Sp.

(Disc diffusion method)

 100 ml Nutrient Agar media is prepared and sterilized by heating

at 100◦c temperature for 20 mints.
 Prepared and heated media is cooled at room temperature and
poured into 5 petriplate and allow to solidify inside the Biosafety
cabinet.
 Disc of whatsman filter papers are incorporated with 0.01%,
0.02%, 0.03%, 0.05% and 0.1% concentration of formaldehyde
and methanol mixture.
 Previously cultured Salmonella sp were spread by spreader over
the 5 petriplates, and Disc are placed on the surface of the Agar
media.
 After 24 hr of incubation at 37◦C result can be concluded.