Translated on 08/22/2022 Page 1 of 18

In-vitro biological effects of environmental W-Fi

and Wi-Max radiation
Preliminary results of research

Research conducted with the financing of the Otto per Mille of the Waldensian Church
by the Chronic and/or Environmental Diseases Association (A.M.l.C.A.)
Authors:
Fiorenzo Marinelli, the CNR Institute of Molecular Genetics, Bologna
Prof. Ian Marc Bonapace, University of Insubria, Busto Arsizio (VA)
Prof. Mario Barteri, Sapienza University, Rome
By Francesca Romana Orlando, journalist and Vice President of A.M.l.C.A.
www.infoamica.it
We thank Dr. Nicoletta Zini and Dr. Despina Kiriakidu of the IGM-CNR of Bologna for the preparation of samples in optical and electron microscopy.
We thank Dr. Monica Mancini and Dr. Martini Mandruzzato of the Department of Theoretical and Applied Sciences of the University of Insubria for having
collaborated in the genetic analysis.
Thanks to Dr. Ivana Lonigro for her collaboration in the experiments in the province of Rome.

We sincerely thank the school administrators, the professors, the library managers and the public administrators who have allowed the realization of this
study, opening the doors of schools and libraries to allow us to take measurements of the field electromagnetic emitted by Wi-Fi systems and to conduct
biological experiments.Special thanks to the Mayor of Pomezia Fabio Fucci who officially joined this initiative working concretely for its realization.
Translated on 08/22/2022 Page 2 of 18

In-vitro biological effects of environmental Wi-Fi and Wi-Max radiation

Preliminary research results 12/31/2014

Research conducted with the funding of the Otto per Mille of the Waldensian
Church by the Association of Chronic and / or Environmental Poisoning Diseases
(A.M.I.C.A.)

Authors: Fiorenzo Marinelli, CNR Institute of Molecular Genetics, Bologna Prof.

Ian Marc Bonapace, Insubria University, Busto Arsizio (VA) Prof. Mario Barteri, La
Sapienza University, Rome

By Francesca Romana Orlando, journalist and Vice President of A.M.I.C.A.

www.infoamica.it

INDEX

1. Introduction page 3

2. Materials and methods page 8

3. Cell viability test results

3.1 Measurements page 10-11

3.2 Proliferation test results pages 4 & 12

3.3 Morphological analysis and electron optical microscopy results page 13

3.4 Wi-Max results pages 4 & 9

4. Epigenetic analysis pages 4 & 9

5. Dosimetry in electro-sensitive page 9

6. Conclusions page 18

7. Bibliography pag. 19
Translated on 08/22/2022 Page 3 of 18

1. Introduction

Wi-Fi means "wireless fidelity" or "wireless fidelity" and represents a data

transmission system through pulsed radiofrequency signals to connect
computers, phones, tablets as well as toys or household appliances and more
generally objects equipped with special microchips for remote control.

Thanks to Wi-Fi it is possible to create wireless Internet access areas, defined

Land Area Networks (LAN) of about 80-100 meters depending on the power of the
antenna, defined as "hot spots".

In the last five years, there has been a widespread diffusion of Wi-Fi modems in
homes and a proliferation of LANs in public places - such as restaurants, hotels,
campsites, beaches, bars, airports, shopping centers, schools and libraries, etc. -
which in fact expose the general population in a ubiquitous way to pulsed
radiofrequency.

Through the radiofrequency of the Wi-Max system which means さ Wi ヴ eless i ﾐ

te ヴ ope ヴ aHility fo ヴ MiI ヴ o┘a┗e AIIess ざ or "Wireless Interoperability for
Access by means of Micro-waves" you can also create wider geographical areas
of wireless Internet access, up to 10-15 km from the antenna. This system is
mainly used for areas that are not reached by cable Internet, such as mountainous
areas or in Third World countries.

The research of A.M.I.C.A. intended to contribute to the understanding of the

biological effects of the radiofrequency emitted by these devices, even at
exposure levels permitted by law.

Specific studies on the effects of Wi-Fi are few, while those in general on radio
frequency are now numerous and outline a risk assessment that is, according to
the authors (already signatories of the Potenza Picena Resolution of 2013),
underestimated by health agencies. publishes nationally and internationally. The
current safety standards, in fact, are based exclusively on the thermal effects of
electromagnetic fields, or on the heating produced on living tissue by these fields.
The scientific literature has now clarified, however, that there are innumerable
biological effects of electromagnetic fields on living matter that occur for levels of
exposure considerably lower than those that can produce heat, that is, they are
non-thermal effects.

An in-depth review of the scientific literature of the non-thermal effects of

electromagnetic fields was conducted by the International Commission for the
Safety of Electromagnetic Fields (ICEMS) 1 by Livio Giuliani, spokesperson for
 Translated on 08/22/2022
Proliferation test results Page 4 of 18

Duration of WiFi Max Measurements of peak magnitudes

Testing barn for the experimental cows

Epigenetic analysis/cell viability

Minus & Plus Contro & Wi-Fi 2.8 GHz Max 24 hour exposure duration Minus & Plus Contro & Wi-Fi 5.8 GHz Max 24 hour exposure duration
Translated on 08/22/2022 Page 5 of 18

ICEMS, former ISPESL research manager, and Morando Soffritti Honorary

President of the Ramazzini Institute of Bologna. This publication was cited as the
prevailing scientific reference by the Resolution of the Plenary Assembly of the
Council of Europe, which will be discussed in the conclusions.

Another reference publication for the analysis of the biological, thermal and non-
thermal effects of electromagnetic fields is the Bioinitiative Report published in
2007 by a group of independent scientists who then published an update in 2012.

1 www.icems.eu. 4

On June 19, 2012, the Russian National Committee for Non-Ionizing Radiation
Protection issued a Recommendation to restrict the use of Wi-Fi in kindergartens
and schools.

From the assessments of these bodies and these groups of independent

scientists it is clear that current international standards are not sufficient to
protect public health and this pushes research to investigate the biological effects
and mechanisms of action even for very low levels of exposure.

Molecular mechanisms that allow to reveal the link between human diseases and
exposure to electromagnetic fields have not yet been definitively established.
statop ヴ esii ﾐ Io ﾐ side ヴ azio ﾐ e p ヴ i ﾐ Iipal ﾏ e ﾐ te the Ialo ヴ e effect and the
ge ﾐ otoxyIhe actions.ReIe ﾐ te ﾏ e ﾐ te, it has been shown that electromagnetic
fields can cause oxidative stress under certain circumstances. Recent results
show that these fields produce alterations of the cell cycle, i ﾐ duzio ﾐ e of ﾏ o ヴ
teIellula ヴ e, ﾏ odification ﾐ of the exp ヴ essio ﾐ ep ヴ oteiIa and, above all,
oxidative st ヴ ess (Oktem F et al. 2005; Caraglia M et al. 2005; Friedman J et al.
2007).

Among the mechanisms of action of electromagnetic fields on living matter at

non-thermal exposure levels, the most significant is the Zhadin effect (1998)
which, through the synergy of radiofrequency and electromagnetic fields at very
low frequencies, involves the opening of the channels of cellular calcium due to
exposure to the cyclotron frequency, and also the increase in many factors related
to oxidative stress which may represent a plausible mechanism for many chronic,
tumor and degenerative diseases found in epidemiological studies (Zhadin M et al.
1998).

A 2012 study in rats raised serious concern about the effects of Wi-Fi exposure on
fertility in fertile organisms and sperm cell integrity. In fact, the study observed
that exposure to Wi-Fi produces an increase in serum levels of 8-hydroxy-2'-
deoxyguanosine and 8-hydroxyguanosine in the testes of the exposed group,
Translated on 08/22/2022 Page 6 of 18

indicating DNA damage due to the exposure to Wi-Fi (p <0.05). At the same time,
lower levels of catalase and glutathione peroxidase were also found in the group
of exposed rats, which could be due to the effect on the enzymatic activity of
radiofrequency (p <0.05) (Atasoy HI et al. 2012).

In the same year, another study showed that the group of subjects who had used
a laptop connected to the Internet with Wi-Fi for 4 hours a day had a significant
reduction in sperm motility and an increase in sperm DNA fragmentation,
compared to the group. not exhibited (Avendaño 2012). This is the first study that
demonstrates the impact of Wi-Fi on human spermatozoa due to a non-thermal
effect of the electromagnetic field.

The damage produced by exposure to Wi-Fi appears to be somewhat recoverable

with melatonin supplementation according to another 2012 study in rats (Oksay T
et al 2012). The rats exposed to Wi-Fi had an increase in lipid peroxidase and a
decrease in vitamin A and E levels which could be prevented by supplementing
with melatonin which also supported the antioxidant system in the testes.

A 2013 study by Aynali G. et al investigated the protective role of melatonin on

oxidative stress induced by Wi-Fi (2.45 GHz) exposure on the laryngotracheal
mucosa of rats. The animals were exposed to 2.45 GHz radiation for 60 min / day
for 28 days. Lipid peroxidase levels were significantly higher (p <0.05) in the
exposed group of rats than in controls and were significantly lower in exposed but
melatonin-treated rats (p <0.01) than in exposed and untreated rats. The activity of
glutathione peroxidase was lower in the group of irradiated and untreated rats
than in controls but was higher in those treated with melatonin (p <0.05). The
reduced glutathione activity did not change in all observed groups of rats. A
possible protective effect of melatonin on the oxidative stress induced by
exposure to Wi-Fi on the mucous membrane of rats can be deduced by inhibiting
the formation of free radicals and supporting the antioxidant system of
glutathione peroxidase.

Since most cancers are now assumed to be activated by damage to the cell's
genome, studies of the effects of electromagnetic fields have mainly been
conducted on DNA and chromosomal structure. Additionally, DNA damage can
lead to changes in cell function and cell death. In 2011, radiofrequency radiation
was classified by the International Agency for Research on Cancer (IARC),
pertaining to the World Health Organization (WHO) as a "possible carcinogen for
humans", or in Class 2B.

A team of researchers led by Prof. Massimo Zucchetti of the Politecnico di Torino

concluded that it would be more appropriate to classify radiofrequency in Class
2A, as a "probable human carcinogen". The article in which this conclusion is
Translated on 08/22/2022 Page 7 of 18

reported is being published in the Journal of Physics.

The identity of the ge ﾐ o ﾏ a is assured by the I ﾐ te action ﾐ and the system of ipa
ヴ or DNA. From an evolutionary point of view, in fact, living organisms have been
equipped with perfect shelter systems and the action ﾐ e da ﾐ osa which is mainly
produced by endogenous factors, such as free radicals, generated by respiration
mitochondrial and metabolism, and DNA polymerase replication errors. Each day
the repair system has to repair about 10,000 endogenous DNA lesions (Lindahl T.
1993), but if exogenous agents, including UV, ionizing and non-ionizing radiation
as well as chemicals, increase the damage dose too much, the system can
undergo aging, cell death or neoplastic degeneration.

The action of the most elect or acting on the identity of the geek has led to
controversial results. Yes ﾐ give a ﾐ ﾐ i 'Γ ヰ of the seIolo yes ヴ so, however,
numerous researchers have shown u ﾐ a Io ﾐ ﾐ essio ﾐ et ヴ a l'i ﾐ I ヴ e ﾏ e ﾐ to of
the ヴ octu ヴﾏ e ﾐ to of DNA I ﾐ exposure ﾐ e to different electromagnetic
frequencies. Lai and Singh reported an increase in single-stranded DNA breaks,
measured with the COMET assay, in brain cells of rats exposed for 2h to 2450 MHz
RFR at a body specific absorption rate (SAR) of 0.6 and 1.2 W / kg. The effects
were blocked by antioxidants, which suggested the involvement of free radicals ﾐ
el ﾏ eIIa ﾐ is ﾏ or action ﾐ e ふ (Lai H, Singh NP. 1995; Lai H, Singh NP. 1996; Lai H,
Singh NP. 1997)).

The ﾐ experiments ﾏ e ﾐ ti performed for ヴ study ヴ and exposure ﾐ and for ヴ te ﾏ

pi lu ﾐ ghi, (Sarkar et al. 1994) exposed mice to 2450-MHz microwave at a power
density of 1 mW / cm2 for 2 h / day for a period of 120, 150, and 200 days. The
authors highlighted re-arrangement of DNA segments in the testes and brains of
exposed animals, suggesting also in this case the rupture of DNA strands after
exposure to the radio frequencies tested (Sarkar S et al. 1994).

"UIIessi┗a ﾏ e ﾐ te, ﾏ olti alt ヴ i ヴ iIe ヴ Iato ヴ i ha ﾐ ﾐ o di ﾏ ost ヴ ato u ﾐ a Io ﾐ ﾐ

essio ﾐ et ヴ to exposure ﾐ and electromagnetic waves and damage to DNA, while
others have failed, as summarized extensively in the 2009 work of Phillips et al
(Phillips JL et al. 2009). The REFLEX study funded by the EU and concluded in
2004 confirmed the results obtained in previous years (F. A. 2005), but
subsequently some articles that appeared in specialized journals partially
disputed the data obtained (Speit G et al. 2007; Speit G. 2014) .

It is necessary to underline, however, that the differences found between the

different articles are often due to non-congruent experimental approaches that
Translated on 08/22/2022 Page 8 of 18

sometimes make it difficult to compare the results achieved by the different

groups.

U ﾐ a possibility is that the from ﾐ ﾐ to the DNA is generated by the ヴ eatti┗e

species of oxygen ﾐ or ふ RO "ぶ ge ﾐ and ヴ atesiall'i ﾐ te ヴ ﾐ or of the cell or oヴ
ga ﾐ is ﾏ o.I ヴ liHe ヴ i adiIals damage cells by targeting macromolecules, such as
DNA, proteins and membrane lipids. Various reports have indicated that the
exposure is to the most elect or to the activity of the cells (Lai H, Singh NP. 1995;
Lai H, Singh NP. 2004; Oral B et al. 2006.), in particular through the Fenton
reaction (Lai H, Singh NP 1995).

The prevailing paradigm for explaining the effects of acute and chronic
environmental toxicity addresses genotoxic and cellular effects. However, more
than fifteen years of studies show that many chronic diseases, including cancers -
including leukemia - and neurodegenerative diseases, are caused by epigenetic
alterations, which affect how genes are regulated at the transcriptional level. post-
transcriptional, translational and post-translational (Sharma S et al. 2010;
Urdinguio RG et al. 2009). It is ﾏ olto i ﾐ te ヴ it ﾐ te ﾐ ota ヴ e Ihe high ヴ action ﾐ i
epige ﾐ etiIhe can ﾐ cause gene mutations at different levels, suggesting that for
some events the modificationsヴ and genotoxic effects. The effect at lu ﾐ go te ヴ
ﾏ i ﾐ and the Hasse doses of electromagnetic fields (and other environmental
pollutants), therefore, could be explained by variations of the epige ﾐ etiIo type, p
ヴ i ﾏ a opa ヴ allela ﾏThe characteristic of epigenetic alterations is that they are
reversible, but in some cases they can also be fixed transgenerationally.

2. Materials and methods

Our investigation started with the hypothesis that the electromagnetic emissions
of Wi-Fi and Wi-Max can cause biological effects on human cells even at levels
below the legal limits and at non-thermal levels, i.e. not capable of producing a
heating of matter. living.

Effects on DNA regulation have been hypothesized with the activation and
repression of some cell cycle control genes and with the presence of possible
damage at the epigenetic level.

The maintenance of life, in fact, depends on the balance of the expression of

apoptotic genes, which control cell death, and of survival, which control its
survival. With apoptosis, particularly damaged cells, which could not survive
Translated on 08/22/2022 Page 9 of 18

Epigenetic analysis

Dosimetry in electro-sensitive
Translated on 08/22/2022 Page 10 of 18

regularly, are eliminated and it is a process that occurs naturally in cellular life,
but can undergo alterations due to environmental electromagnetic exposures.
Previous publications have shown that there is an activation threshold for these
genes by the power of the electromagnetic field and by the time of exposure.

To verify these mechanisms, experiments were carried out by exposing cells to

the electromagnetic field of Wi-Fi at 2437 MHz with power density -27 dbm in the
laboratory. The first experiments were carried out on primary cultures of healthy
muscle cells taken from biopsy which, exposed for short times, from one hour to
six hours, showed apoptotic activation and a decrease in cell proliferation. When
they were exposed for over 12 hours, they showed a statistically significant
increase in cell proliferation.

TABELLA PROLIFERAZIONE CELLULE TABELLA PROLIFERAZIONE CELLULE

MTT TEST (tempi lunghi) MTT TEST (tempi brevi ) SURVIVAL
Translated on 08/22/2022 Page 11 of 18

3.1 Measurements

The legal limits in Italy for fixed antennas, such as Wi-Fi hot spots, is 6 V / m. For
mobile devices connected to Wi-Fi (which themselves become antennas but
mobile) there is no ministerial decree indicating specific limits in implementation
of the Framework Law on electrosmog 36/2001. We therefore refer to the European
regulation of 2 Watt / Kg of SAR.

Studies report measurements of significant levels of radiation in the immediate

vicinity of Wi-Fi routers, i.e. access points to Wi-Fi and in the vicinity of laptops
connected to Wi-Fi: for example, in 2010 the Ecolog Institute found at 20 cm from
a Wi-Fi router 8.8 V / m = ヲ ヰ ヵ, ヰ ヰ ヰ μW / ﾏ ², and at the same distance from a
Wi-Fi access point, 7.5 V / m = 1 ヴ Γ, ヰ ヰ ヰ μW / m²; the IMST study (2005) has ﾏ
isu ヴ ato ヲ Α, ヰ ヰ ヰ μW / ﾏ ² (8.7 V / m) 50 cm away from a laptop. According to
the Building Biology Evaluation Guidelines, these levels (over 1,000μW / ﾏ ² = 0.65
V / m) are ﾐ o Ilassified Io ﾏ e u ﾐ a さ est ヴ e ﾏ a p ヴ eoIIupazio ﾐ e. ざ I ﾐ pヴ
existence of many users (for example, 20 students in a classroom) exposure
levels are even higher.

Compared to this data, our measurements reported rather low levels of emission
from Wi-Fi routers. The average of all the measurements we make in libraries and
schools is 0.65 V / m, equal to just over a tenth of the legal limit (6 V / m).

It should also be considered that, at the time of our measurement, the Wi-Fi
antennas were not in fact fully operational in their use as there was not yet the
provision of tablets in the schools, which is instead provided for by the current
training digitization policies.

It is easy to predict that in the future, when there are 15, 20 or 25 tablets
connected to the Wi-Fi router in each class, the summed emissions between Wi-Fi
router and tablet will be tens of times higher than those detected by our
measurements.

In our opinion it will be useful to replicate in the future the same type of research
we conducted this year in schools that equip each student with a tablet connected
via Wi-Fi to the electronic board, placing the incubator on one of the benches to
study the genetic effects of environmental exposure that presents the sum of the
Translated on 08/22/2022 Page 12 of 18

emission deriving from the Wi-Fi router (greater than that found by us as it serves
several devices at the same time) and from the same mobile device which in turn
functions as a receiving and transmitting antenna.

MEASUREMENTS OF THE ELECTROMAGNETIC FIELD EMITTED BY WI-FI

ROUTER

* Places where measurements were made.

3.2 Proliferation test results

In the high school in the province of Bolzano, the cells exposed to a Wi-Fi field of
0.75 V / m for 48 hours showed a significant decrease in cell viability compared to
controls, a phenomenon that did not occur after a 24-hour exposure. .

In the other upper secondary school in Bolzano, no appreciable differences

emerged in the viability of exposed cells and control cells after 24 and 48 hours of
exposure.

Also for the two schools in the province of Rome, no significant differences were
found regarding the proliferation of exposed and control cells, but it should also
be specified that the signal was given that it was limited to the value of the carrier
(0.16 V / m) given that the Wi-Fi broadcaster, although active, was not used by the
students' tablets or computers and, in fact, only the basic signal was present.

In the experiments carried out in the library in the province of Bologna, on the
other hand, significant differences in cell viability were found between the
exposed and control samples, as evidenced by the image above in which the
control wells show a more intense color than those of the controls. of exposed
cells, indicating that exposure caused a decrease in cell viability in all cases, for
both 24 and 48 hours of exposure.

In summary, with regard to cell viability analyzes, 4 of the 7 experiments with

cultured cells in public settings, i.e. schools and libraries, showed proliferation
alteration effects due to exposure to Wi-Fi at significantly lower levels than those
predicted by the legal limits.
Translated on 08/22/2022 Page 13 of 18

3.3 Morphological analysis and electron optical microscopy results

Library in the province of Bologna WI-FI 24h

Library in the province of Bologna WI-FI 48h

Morphologically significant differences are evident between the control cells (C in

the label) and the exposed cells (E in the label) at both 24 and 48 hours. At 48
hours of exposure the cells appear to be particularly degraded with evident signs
of alteration of the nuclear membrane. All exposed cells have evident thickened
nucleoli and a greater number of micronuclei.

3.4 Wi-Max results

Our observation also concerned Wi-Max emissions, whose frequency is 5.8 GHz.
Field tests were carried out at a farm in the Bolognese Apennines which has a Wi-
Max antenna installed about 35 meters from the barn. where the cows stay.

Also in this case, cell cultures were carried out using a portable incubator which
gave significant differences in cell viability between the exposed and control cells.
As can be seen in the photograph below, after 24 hours of exposure, in the control
wells (C) there is a greater vitality than in the exposed ones (E). The darker color,
in fact, indicates that the vital dye has been metabolized by live cells which are to
a greater extent than the lighter control wells.

1 to 7 are control HL60 cells. 8 to 14 are exposed HL60 cells. 17 to 22 are control
CEM cells.

As can be seen in the exposure measurement graph, by chance the

electromagnetic field emitted by the antennas had increased up to 5.2 V / m, a sign
of sudden changes in emissions during use by mobile devices.

4. Epigenetic analyzes

Epigenetic control is exercised at at least three levels: DNA methylation (and other
modifications), histone modifications and non-coding RNA. In particular, the
methylation state of DNA is controlled at different levels by numerous enzymes
capable of adding or removing the modification. DNMT ヱ, DNMT ン A and DNMT ン
Translated on 08/22/2022 Page 14 of 18

B and UHRF ヱ so ﾐ or Ioi ﾐ ┗olte ﾐ in the addition of a methyl group, while TET1,
TET2 and TET3 are involved through a complex mechanism, which also involves
repair processes of DNA, in the removal of methyl groups.

In the present study, ┗oluto a┗┗ia ヴ e u ﾐ 'i ﾐ dagi ﾐ and on the ┗a ヴ iazio ﾐ i of esp
ヴ essio ﾐ and ge ﾐ i IheIo ﾐ t ヴ olla ﾐ or the ﾏ ethylation ﾐ edel DNA i is exposed to
the exposure and to the pielect or agents generated by WiFi. In particular, the
levels of DNMT1 and UHRF1 were evaluated. We also evaluated the expression
levels of Caspase 3 to evaluate the possible activation of apoptotic processes. It
was performed in ﾐ Iheu ﾐ ap ヴ i ﾏ a ﾏ isu ヴ adi ﾏ ethylation ﾐ and gloHaledel ge ﾐ
o ﾏ a, using LINE1 sequences as a surrogate for global methylation.

Unfortunately, the two samples of the exhibitions at 24 and 48 hours of the high
school in the province of Bolzano and of the ヲ ヴ o ヴ and of the exhibition ﾐ e and
of the I ﾐ t ヴ ollo and of the exhibition ﾐ and a ヴ Β of the Institute "upe ヴ io ヴ e ﾐ
ellap ヴ o┗i ﾐ Iiadi Bolza ﾐ o ed of the exhibition ﾐ and 48 hours of high school in
the Province of Rome, were quantitatively inferior to the other samples. This
limited the possibility of making a comparative evaluation of the samples.

The analysis of the exp ヴ essio ﾐ et ヴ asI ヴ izio ﾐ ale of the ge ﾐ i IodifiIa ﾐ ti the
same p ヴ otei ﾐ and ﾐ o ﾐ was

possible as the RNA samples extracted from the cells were completely degraded

or absent. No ﾐ was, du ﾐ ケ ue, possible p ヴ oIede ヴ and Io ﾐ l’a ﾐ alisi pe ヴ PCR

of essio ﾐ e ge ﾐ iIa.

From the Western blot data it is therefore not possible to conclude that there is or
is not an alteration in the expression of the UHRF1, DNMT1 and Casaspase ン i ﾐ
genes following exposure ﾐ and electromagnetic fields generated by WiFi
systems.

In order to obtain statistically significant results, it is necessary to carry out

further experiments in the field, also to identify the genetic markers most sensitive
to exposure to radiofrequency radiation.

5. Dosimetry in electro-sensitive

Dosimetry in electrosensitive patients has allowed to verify in some patients that

exposure to electromagnetic fields in living environments involves the onset of
symptoms characteristic of hypersensitivity. In particular, this was highlighted for
DECT telephone exposures and GSM exposures.
Translated on 08/22/2022 Page 15 of 18

The statistical analysis of the data collected will allow to evaluate the actual
correlation between symptoms and exposure.

Graph 1. Graph of the recording of radiofrequency electromagnetic emissions

carried out on a patient with electrosensitivity characteristics, which shows the
emissions at different frequencies, highlighted with different colors of GSM
telephony (brown uplink, red downlink), GPRS (dark green uplink, green clear
downlink), UMTS (clear uplink, blue downlink), DECT (burgundy) and Wi-Fi
(purple). In this case we observe the markers (blue squares) that identify the
moment in which the patient perceived the symptoms of electrosensitivity, which
in this case are particularly correlated with the signal power density).

The observation of these dosimetric measurements has also made it possible to

highlight some characteristics of the type of exposure, showing that it is above all
GSM telephony that presents very high peaks, even higher than 100 V / m at the
time of connection with the radio base station, or at the time of activation of the
phone call.

It was also noted that three patients asked to remove the dosimeter before the end
of the scheduled 24 hours as they believed that the same device was causing their
symptoms worsening.

6. CONCLUSIONS

Marinelli F, La Sala D, Cicciotti G, Cattini L, Trimarchi C, Putti S, Zamparelli A,

Giuliani L, Tomassetti G, Cinti C., Journal of cellular physiology, 198: 324–332
(2004).

Nazı ヴ oglu et al, 2.45GHz electromagnetic radia The published literature on the
biological effects of Wi-Fi already offers indications on the multiple effects that
this type of emissions can have at the cellular and enzymatic level, suggesting the
need to apply a precautionary principle in exposures .

Our research aimed to identify a possible mechanism of action, at the cellular

level, finding that indeed some proliferative and epigenetic genetic effects also
appear at non-thermal levels of exposure.

To reach definitive conclusions on the possible genetic and epigenetic effects of

environmental exposure to Wi-Fi and Wi-Max frequencies it is necessary to carry
out further experiments to have consistent data beyond these preliminary results.

7. BIBLIOGRAPHY
Translated on 08/22/2022 Page 16 of 18

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Aynali G et, Modulation of wireless (2.45 GHz) -induced oxidative toxicity in

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270 (5): 1695-700. doi: 10.1007 / s00405-013-2425-0. Epub 2013 Mar

12. https://www.ncbi.nlm.nih.gov/pubmed/23479077

Atasoy HI, et al. Immunohistopathologic demonstration of deleterious effects on

growing rat testes of radiofrequency waves emitted from conventional Wi-Fi
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Avendaño C, et al. Use of laptop computers connected to internet through Wi-Fi

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