STERILIZATION, CULTURE MEDIA AND PURE CULTURE

TECHNIQUES

1. Medium used in Membrane filtration is EMR-Vp Medium.

2. Lysol is Disinfecting agent.
3. Neutral strain is Neutral Red.
4. Peptone water medium is Semi synthetic based medium.
5. Method in which cells are dried frozen is called as Lyophilization.
6. Technique used for prevention of all types of microbes in general is called as Sterilization.
7. Thermal death is defined as Temperature that kills all cells in given time.
8. Culture medium where exact composition is not known is said to be Simple culture.
9. Elek’s Gel Diffusion test is used for detection of Diphtheria toxin.
10. Temperature required in Pasteurization is <100 C.
11. Separations of Single bacterial colony is called as Isolation.
12. Ionizing radiation is Gamma rays.
13. Dimerization of Thymine is caused by UV rays.
14. As food materials are preserved/stored just above their freezing temperature it is called as
Chilling.
15. Method that is not suitable for killing of microbes is Drying.
16. Treponema pallidum can be best seen via Bright field microscope.
17. Autoclaving is done at 120C.
18. Temperature in Pasteurization is 62.8C
19. Bacterial culture prepared by Pure culture method is called as Inoculum.
20. Algae are rich in vitamins, proteins and carbohydrates.
21. L-Lysine is prepared from Corynebacterium glutamicum.
22. Orderly increase in quantity of all cellular components is called as Growth.
23. Theo bacillus Thi oxidans can grow at 1C.
24. Slow freezing requires temperature of -15 to 3C for 72 hours.
25. Discontinuous heating process is called as Tindalization.
26. Isolation is separation of Single colony.
27. Conditions required for Autoclaving is 120-150C for about 1 hour.
28. Lysozyme is effective against Gram+ bacteria.
29. Blood agar medium is Enriched medium.
30. Infra-red method of killing microbes is Mechanical method.
31. Lyophilization is Freeze drying.
32. Temperature for Hot air oven is 150-160C for 1 hour.
33. Phenol Co-efficient means Efficiency of Disinfectant.
34. Method used for Estimation of bacteria count in milk as a Agar plate method is also known as
Standard Plate count method.
35. Agar is obtained from Red algae.
36. Gram+ bacteria that produces swarming on culture medium is Proteus.
37. Enhancing virulence of bacteria is called as Exhalation.
38. For effective sterilization in Autoclave temperature required is 120C
39. Spores are killed by Glutaraldehyde and Autoclaving.
40. Glass wares are sterilized by Hot air oven.
41. Tyndallization was introduced by Tyndall.
42. Virus can be cultivated in Living cells.
43. By pasteurization, microbes are killed only that are pathogenic in nature.
44. Temperature required for Pasteurization is below 100C.
45. In medium other than nutrients, if it is in excess quantity it is said to be Enriched medium.
46. Example of Indicator mediums is Wilson and Blair’s.
47. Example of Anaerobic medium is Robertson’s cooked meat medium.
48. To differentiate Lactose and Non lactose fermenters, medium used is Mac-Conkey’s Agar
medium.
49. Best method to obtain Pure culture is Streak Plate method and Agar Slant method.
50. To transfer medium from one place to another, device used is called as Needle.
51. Bacterial culture prepared by pure culture is called as Inoculum.
52. Separation of single colony is called as Isolation.
53. Growth period of culture is called as Incubation.
54. At 150C for 1 hour, complete sterilization is done by Hot Air oven.
55. In Autoclave, Principle involved is Moist air and Steam under pressure.
56. Spores of bacteria that can withstand moist heat effect is of Bacillus stearothermophilus.
57. Factors on which disinfectivity of disinfectant depends is/are Concentration of a substance,
Time of action, Ph of medium and suitable temperature.
58. Most powerful disinfectants among aldehydes are Formaldehyde and Glutaraldehyde.
59. Accridine dye is most suitable for Gram+ bacteria.
60. Sterilizing agent is Ethylene oxide.
61. Disinfectant salts of heavy metals are Thiomersal nitrate, Phenyl mercury etc
62. Cultures that are prepared by penetrating inoculation loop with suspension into medium is
called as Sub-culture.
63. Principle involved in Streak plate method is Dilution method.
64. Culture medium for Fungi are Potato Dextrose Agar (PDA), Sabourauod’s medium and
Czapek dox agar.
65. Spores of Actinomycete are very sensitive and are killed at room temperature for 65C for 30
mins.
66. Bacteria that can withstand pasteurization but can’t grow at higher temperatures are known as
Thermoduric bacteria.
67. Common method for cultivating anaerobic microorganism is Pyro-gallic acid over cotton.
68. Alkaliphiles grows at PH of 7 to 12.
69. Microorganism that grows at High salinity are Halophile and Osmophile.
70. Non lactose fermenting colonies seen on Mac-Conkey’s medium are of Salmonella typhi.
71. Wilson and Blair medium is used for Isolation of Salmonella typhosa.
72. Laboratory diagnosis of Enteric fever is Stool test, urine test and Widal’s test.
73. Shigella was first isolated by Sonnei.
74. Gas producing Salmonella is S.enteritidis.
75. Kauffman white scheme method is used to detect Salmonella sp.
76. On Mac-Conkey’s medium, E.coli forms Pinkish colonies.
77. Method required for Clostridium diphtheria is Potassium tellurite medium.
78. Medium required for Myobacterium tuberculosis is Lowenstein Jensen (LJ) method.
79. Lepra bacillus is best cultured on Footpad of mice.
80. Medium required for Clostridium sp. Is Robertson’s cooked meat method.
81. Clostridium welchii is Positive for Nagler’s Test.
82. Nagler’s Test detects Lecithinase.
 83. Incubation period for C.welchii is 8-12 hours.
84. Average incubation time of tetanus is 7-14 days.
85. Salt agar is used for Staphylo coccus sp.
86. Culturing medium for Leshminia is NNN method.
87. Simple Asexual spore that is formed by Budding is known as Blastophore.
88. Sabourauod’s method is used for Fungi.
89. For sterilization of equipments methods used is/are Heat, Chemical and Radiation.
90. Assays of microorganisms

Crystal violet Lactobacillus aureus

Ampicillin Micrococcus luteus

Plague vaccine Pasteurella sp.

Rifampin Bacillus subtilis

91. Formulations

Lysol Solution for ceresol with soap.

Black fluids Higher boiling fractions of Tar acids.

White fluids Prepared from Refined Tar acids.

Iodophores Iodine combined with Complex organic

compounds

92. Applications

Shick Test Diphtheria toxin.

Montoux Test Tuberculosis
Sterility Test Detection of Extraneous microorganisms.

Potency Test Usefulness of Immunological products.

93. Method of sterilization

Glass syringes Dry heat

Disposable items Gamma Radiations

Respiratory parts Chicken pox in children.

Dialysis equipments By Chemicals

94. Methods used are as

Coomb’s Test Non-agglutinating bodies.
Coagulase Test Candida albicans
95. Diphtheria pneumonia can be cultivated in Chocolate Agar, Glucose broth, Serum Broth and
Agar broth.
96. ..
97. Diagnosis of Tuberculosis is carried out by Emulator, Antiformin method, Petroff’s method
and Concentration method.
98. Size of Virus can be determined by Micrography, Ultra centrifugation, Ultra filtration.
99. Differential straining of bacteria is related to Moller’s straining.
100. Electron Microscopy do not help in studying Corpuscle of spores of microorganisms.
101. Wilson and Blair medium is used for Salmonella typhi.