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Protease Inhibitors and Phosphatase Inhibitors  PMSF Protease Inhibitor

Thermo Scientific™

PMSF Protease Inhibitor

Catalog number: 36978
Related applications: Protein Purification & Isolation

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Catalog Number 36978

Unit Size 5g

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Product Overview Documents

Thermo Scientific PMSF is a protease inhibitor that reacts with serine residues to
inhibit trypsin, chymotrypsin, thrombin and papain.

Features of PMSF:

• Targets serine proteases, including trypsin, chymotrypsin, thrombin and papain

• Effective in most protein solutions at 0.1 to 1 mM final concentration

• Because PMSF has limited water solubility, it must be dissolved in a small amount of
solvent, such as ethanol, methanol or isopropyl alcohol before addition to a buffer

 PMSF is an abbreviation for phenylmethylsulfonyl fluoride, the most common

chemical name for this small compound. In addition to inhibiting serine proteases,

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PMSF will also inhibit cysteine proteases, like papain (reversible by DTT treatment),
and mammalian acetylcholinesterase.

Properties of PMSF:

• Synonyms: alpha-Toluenesulfonyl fluoride; Benzylsulfonyl fluoride;

Phenylmethylsulfonyl fluoride

• Formula: C6H5CH2SO2F

• Molecular weight: 174.19

• CAS Number: 329-98-6; EC Number: 206-350-2

• R/S Codes: R 25-34; S 26-36/37/39-45

• Storage conditions: room temperature in a dry place protected from light

• Working solution: dissolve PMSF in isopropanol at 1.74 mg/mL (10 mM); store in
aliquots at -20°C.

Specifications of PMSF (Part No. 36978):

• Appearance: white powder

• Purity: > 99.0 %

• Melting point: 90-95°C

• Humidity: < 0.1 %

Related Products

Pierce™ Protease and Phosphatase Inhibitor Mini Tablets, EDTA Free

Pierce™ Protease Inhibitor Mini Tablets, EDTA Free

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Inhibitors Protease Inhibitor

Quantity 5g

Form Powder

Reagent Type Protease Inhibitor

Description Pierce PMSF Protease Inhibitor

Product Type Inhibitor

Contents & Storage

Store in original container protected from direct sunlight in a dry, cool and
well-ventilated area, between the following temperatures: 20°C to 25°C.

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Lot # Certificate Type Date

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 XG355221
Analysis
Jul 23, 2022 36978

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 XF355220
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Jul 08, 2022 36978

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 XE355218
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Jun 01, 2022 36978

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Jun 01, 2022 36978

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 A0441743
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May 02, 2022 36978

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