laboratory

exercise II

GROUP 1 (FROGGLE
BUNWICH)
BS BIOLOGY 1-YA-1
Ms. Lascano
09/02//2022
 Exercise 2
objectives:
1. To be familiar with the parts, basic set up, proper ways of
using, troubleshooting and cleaning the microscope.
2. To strengthen the value of responsibility
3. To enhance personal and communication skills

FROGGLE PROGRAM AND BS BIOLOGY

group # 1
BUNWICH SECTION 1-YA-1

Provided input for questions on part A. (11-12) and B.

Christian P. Amog (Leader) (4), provided visual documentation, managed the role of
groupmates

Played Simulation and provided input and answers for

Shobe Cabrera (Leader) questions on part A. (10) and part B. (2-3)

Played Simulation and provided input for questions on

Vonn Clarence Bate (Arbitrator) part A. and B.

Played Simulation and provided input and answer for

Argeff Jersey Castillo (Arbitrator) question on part B. (1)

Played Simulation and provided input and answers for

Belyn Mae Cianco (Note taker) question on part A. (7-9)
 Played Simulation and provided input and answers for
Julius Belarmino (Note taker) question on part A. (1-3)

Francene Aleli Cañares (Quality Played Simulation, designed and created template for
pdf exercise, encoded answers of members in the
Control) template, and answered question on part A. (10)

Melyssa Candelario (Quality Played Simulation, provided answers and input for
Control) question on part A. (4-6)

Lab Performance
rubric
DOCUMENTARY
DOCUMENTARY
 A.
Label the parts of the microscope.
Encode your answers on the table
provided below

parts functions

is what you look through at the top of the

microscope. The eyepiece magnification of
the eyepieces in Figure 1 is 10X, as indicated
on the housing. The inscription A/24 indicates
the field number is 24, which refers to the
1. Eyepiece or Ocular diameter (in millimeters) of the fixed
diaphragm in the eyepiece. These eyepieces
also have a focus adjustment and a
thumbscrew that allows their position to be
fixed.

The microscope body tube separates the

objective and the eyepiece and assures
continuous alignment of the optics. It is a
standardized length, anthropometrically
2. Body related to the distance between the height of
a bench or tabletop (on which the
microscope stands) and the position of the
seated observers.


Supports the tube and connects it to the

base. Base: The bottom of the microscope,
used for support. Illuminator: A steady light
3. Arm source (110 volts) used in place of a mirror. If
your microscope has a mirror, it is used to
reflect light from an external light source up
through the bottom of the stage.

Also known as the revolving turret. It holds the

objective lenses and attaches them to the
4. Nosepiece microscope head. It is movable hence it can
revolve the objective lenses depending on the
magnification power of the lens.

It is designed to directly observe the object.

Each objective lens has different
magnifications, such as a scanning lens(4x),
low-power lens(10x), mid-power lens(40x),
5. Objective Lens and high-power lens(100x). The objective lens
then focuses reflected light from the object
up a tube toward the ocular lens, which is the
lens the examiner looks through.

A disc-shaped device, it is used to control the

amount of passing light through the stage
opening. The diaphragm is located directly
under the stage or platform where the user
places the specimen or slide. The diaphragm
disc, sometimes called an iris, has tiny holes in
6. Diaphragm it that let varying degrees of light in under the
specimen. By opening the diaphragm, an item
that at first appears too dark is easier to
observe. Adjusting the diaphragm can also
create contrast for better viewing of
transparent specimens.

the main, flat plate that holds and supports

7. Stage the slides for observation, together with the
stage clips that holds the slides in place.
 located beneath the stage to control the
8. Stage adjustment knobs forward, reverse and side to side movements
of the stage.

This is the part of the microscope which

controls the power, commonly in the form of a
9. On and Off switch push-button located on the microscope's
base.

The coarse adjustment knob or coarse focus

can be found on the left side of the
microscope, together with the fine
adjustment or fine focus knob. It's also
described as the knob larger than the fine
focus. The main function of the coarse
Coarse adjustment knob adjustment knob is to move the objective
10. lenses closer or farther away from the
or Coarse Focus
specimen in large steps. In addition to this,
the coarse knob is also notably used to
initiate scanning and low power when using
the microscope. This knob plays an important
role in making sure initial procedures are met
to transition to higher power objectives.

This one is the smallest of the two adjustment

knobs that you can interact with in a
microscope. It is utilized to bring the
objective lenses nearer to or further from the
specimen while maintaining a very close
11. Fine adjustment knob proximity. This adjustment knob is used to
fine-tune the focus on any area of a
specimen after first adjusting the focus with
the coarse adjustment knob. It is strongly
advised to use this knob only for higher
magnifications.

This is the microscope's supporting structure.

It is the point at which the arm extends to
meet the upper part where the eyepiece is
12. Base located. The illuminator can be found at the
top of the base. To move a microscope, place
one hand beneath the base and the other
holding the arm.
 Watch the video entitled “Microscopes: Basic-

B.
Setup”
Summarize your learning on the provided table
below
Indicate the name/s of member/s who
contributed

topics learning contributors

In order to set up a microscope, one must first acquire a

microscope from the laboratory cabinets or from the laboratory
technicians. It is principal to handle the microscope with utmost
care. When handling the microscope, always use both hands,
one holding the arm and the other under the base of the
instrument. Always remember to ask for assistance if the
microscope is too heavy. To turn on the microscope, you need to
Setting up plug its cords into an outlet, then locate the ON/OFF switch at Argeff Jersey
the back of the scope and switch it to ON. Afterwards, rotate
the the ocular lenses (eyepiece) until the number 64 lines up with
Castillo
microscope the white line beneath the rotating part of the lens. Next, (Arbitrator)
calibrate the condenser lens by rotating its adjustment knob,
then elevate it so it is directly under the stage. Set the scanning
objective lens (4X) directly over the stage, then elevate the
stage to its highest position. Lastly, adjust the control knob of
the light and set it to the medium or medium-high range. We
can always adjust the light later to get a better view of the
image.

To start using the microscope, the clip on the stage must be

opened wide enough to fit a microscope slide which must be
pushed to the back of the stage and held into place by the clip
but not pushed too much as to slide under the clips. Next, the
specimen on the slide must be centered over the condenser lens
by using the knobs on the lower side of the stage to move the

slide to its proper position. Once the specimen slide is settled,

Using the start looking first through the specimen using the low power Shobe Cabrera
microscope objective lens (4X) with the stage in its highest position, making (Leader)
use of the ocular lens to locate and focus on the specimen by
turning the coarse adjustment knob. Once the specimen is

centered in the field of view, it is proper to move to the next

objective lens (10X) and refocus with the fine adjustment knob.
Again, the specimen must be centered before it is deemed
proper for the highest power objective lens (40X) for refocusing
for sharper, clearer details.
 In the process of using the microscope you might come across
some potential issues that require troubleshooting. If the slide
does not move when it is being adjusted by the slide holder,
check if you are using the right knobs and if the slide is properly
and sturdily clipped in place. If you are having difficulty focusing
on the specimen, make sure that the specimen is centered first
and then start with the objective lens with the lowest power and
the stage in its highest position. Gradually make minor
Trouble adjustments only as not to get frustrated and eventually get too
far out of focus. You may also move the slide using the slide Shobe Cabrera
shooting holder knobs when focusing on the specimen. When observing (Leader)

unstained specimens, reduce the amount of light passing
through with the use of the stage diaphragm to better focus on
the specimen. You may also try locating easily observed objects
within the unstained specimen for easier focusing. If the
specimen becomes blurry as you move to a higher power lens,
wipe the lens first with a lens paper that was soaked with lens
cleaner, then wipe the lens dry with clean less paper after.
Repeat this a couple of times and if it doesn’t resolve the issue,
consult with the instructor.

After using the microscope, simply remove the slide from the
stage by loosening the stage clips. To change the magnification
of the objective lens, one must rotate the revolving nosepiece.
Clean Check that the stage is clean and dry, and that the slide holder Christian P. Amog
up is positioned to the left. Turn off the light and power, then lift it (Leader)
with two hands back to its storage location, making sure the arm
is facing outward and the microscopes are not cluttered
together.