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1. Sample collection from dam
water placed in sterile plastic
container and transported in an
iced box when transferred in the
laboratory.
CASTRO, NAJELA LEILA D.
BSBIO 3-3MB
LONG EXAM #2
8. Primary identification using
differential medium such as
Triple sugar iron test (TSI) agar
and oxidase test.
9. When the observed colony of
bacterium is discovered to be
unknown, morphological, and
physiological differentiation
should be performed.
10. Once identified and
characterized as novel species,
preparation for preservation should
be performed. Bacterium colony
should be stored in a selective
medium with specific
cryoprotectant such as skim milk or
other animal sera.
2. Serial Dilutions of water
sample when transported in the
laboratory.
7. Test for antimicrobial
susceptibility using Kirby-Bauer
disk diffusion method.
9.1 Morphological differentiation
- Endospore staining
- Acid-fast staining
- Capsule staining
9.2 Physiological differentiation
- Biochemical test
- Motility test
- Growth requirement
11. Culture of novel species of
bacterium must be prepared
for cryogenic preservation.
Lyophilization under -20°C that
last for at least a year.
3. To test for microbial 4. Once Filtration is done, the
contaminant, Membrane
filtration should be performed.
6. Purification of each observed
colony by twice subculturing
using streak-plate method.
Isolation, Identification,
and Preservation of
Novel Species of
Bacterium from
Water Sample.
12. Preparation and finalization
of important information
regarding the species of
bacterium.
membranes should be placed in a
specific selective media.
Selective media for identification of
- coliform (mFC agar) 45°C 24hrs
- heterotrophy (nutrient agar) 37°C
24hrs
5. Enumeration, characterization,
and differentiation of colonies
formed.
13. Submission of data to various
Culture Collections Centers in the
Philippines and if possible
international. As well as
verification from them.