Columbus Washer

Technical Manual for
Columbus Washer
Document Part No.: 30000294

2006-02
Document Revision No.: 1.7
Firmware Level: 4.xx
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2 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 2006-02
WARNING
CAREFULLY READ AND FOLLOW THE INSTRUCTIONS
PROVIDED IN THIS MANUAL BEFORE OPERATING THE
INSTRUMENT.
Notice
Every effort has been made to avoid errors in text and diagrams, however, Tecan
Austria GmbH assumes no responsibility for any errors which may appear in this
publication.
It is the policy of Tecan Austria GmbH to improve products as new techniques
and components become available. Tecan Austria GmbH therefore reserves
the right to change specifications at any time.
We would appreciate any comments on this publication.
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AUSTRIA / EUROPE
Telephone: 0043 (0)6246/8933
FAX: 0043 (0) 6246/72770
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www.tecan.com
Copyright Information
The contents of this manual are the property of Tecan Austria GmbH and are not
to be copied, reproduced or transferred to another person or persons without our
prior written permission.
Copyright © Tecan Austria GmbH
All rights reserved.
Printed in Austria.
Declaration for EU Certificate
See the back of this manual.
About this Manual
This technical manual describes the servicing of the Columbus Washer, designed
to wash microplates. It is intended only for service personnel.
This manual instructs how to:
• Install the instrument
• Operate the instrument
• Programming the wash procedure
• Definition on the wash parameters
• Cleaning and maintenance procedures
2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 3
Warnings, Cautions and Notes
There are three types of informational notices used in this manual. These notices
highlight important information or warn the user of a potentially dangerous
situation. The following notices are:
Note
Gives helpful information.
Caution
Indicates a possibility of instrument damage or data loss if instructions are
not followed.
WARNING
INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS OF
LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT
FOLLOWED.
Table of Contents
1. General
1.1 Overview......................................................................................... 1-1
1.2 Instrument Description ................................................................. 1-2
1.2.1 Rear Panel Connections.................................................................. 1-2
1.2.2 Connection Diagram for Columbus (1 Liquid Channel)*.................. 1-4
1.2.3 Connection Diagram for Columbus (4 Liquid Channels)* ................ 1-5
1.2.4 Keyboard and Display Unit .............................................................. 1-6
1.3 Interconnection Diagram .............................................................. 1-7
2. Installation Procedure
2.1 Unpacking and Inspection ............................................................ 2-1
2.2 Unpacking Procedure ................................................................... 2-2
2.3 Power Requirements ..................................................................... 2-2
2.4 Installation Procedure ................................................................... 2-3
2.4.1 Installing the Instrument .................................................................. 2-3
2.4.2 Connecting the Waste Tubing ......................................................... 2-3
2.4.3 Connecting the Solution Tubes ....................................................... 2-4
2.4.4 Fitting and Connecting the Liquid Sensors (Optional) ..................... 2-5
2.4.5 Connecting the Barcode Reader (Optional)..................................... 2-6
2.4.6 Connecting the Printer (Optional) .................................................... 2-6
2.4.7 Power Connections ......................................................................... 2-6
3. Firmware and Software Description

3.1 Instrument Software Features ...................................................... 3-1
3.2 Onboard Software Menus ............................................................. 3-2
3.2.1 Run .................................................................................................. 3-2
3.2.2 Prime ............................................................................................... 3-2
3.2.3 Rinse ............................................................................................... 3-3
3.2.4 Program........................................................................................... 3-6
3.2.5 Clear ................................................................................................ 3-6
3.2.6 Show................................................................................................ 3-6
3.2.7 Adjust............................................................................................... 3-7
3.2.8 Service Menu................................................................................. 3-24
3.3 External Software ........................................................................ 3-61
3.3.1 Columbus Computer Software ...................................................... 3-61
3.3.2 Firmware Update ........................................................................... 3-61
3.4 Serial Interface............................................................................. 3-62
3.4.1 Hardware Specifications................................................................ 3-62
3.4.2 Contents ........................................................................................ 3-65
3.4.3 A. Protocol ..................................................................................... 3-67
3.4.4 Sequence Number......................................................................... 3-68
3.4.5 B. Commands - Overview.............................................................. 3-69
3.4.6 Commands - Description ............................................................... 3-71
3.4.7 D. Examples for Remote Control - Command Sequences.............3-88
3.5 Description of Error Messages ...................................................3-95
4. Removing and Replacing the Instruments Cover

4.1 Removal of Instruments Top Cover..............................................4-1
4.2 Replacing the Instruments Top Cover .........................................4-3
5. Liquid System
5.1 Introduction ....................................................................................5-1
5.1.1 Liquid System Diagram ....................................................................5-2
5.1.2 Dispensing System Description........................................................5-3
5.2 Replacement Procedure ................................................................5-4
5.2.1 Replacing the Dispensing Pump ......................................................5-4
5.2.2 Replacing the Solution Solenoid Valves (Instruments with Pinch
Valves) .............................................................................................5-6
5.2.3 Replacing the Solution Solenoid Valves...........................................5-7
5.2.4 Replacing the Dispense Solenoid Valve ..........................................5-8
5.2.5 Replacing the Aspirating Pump ........................................................5-9
5.2.6 Replacing the Aerosol Pump..........................................................5-10
6. Mechanical System
6.1 Manifold Arm Movement System..................................................6-1
6.1.1 Description of Manifold Arm Movement Parts ..................................6-2
6.1.2 Adjusting the Manifold ......................................................................6-2
6.2 Plate Transport System .................................................................6-3
6.2.1 Plate Support ...................................................................................6-4
6.2.2 Leveling the Plate Support ...............................................................6-5
6.2.3 Microplate Positioning System .........................................................6-7
6.3 Replacement Procedure ................................................................6-8
6.3.1 Replacing the Manifold Arm Error Sensor........................................6-8
6.3.2 Replacing the Motor .........................................................................6-9
6.3.3 Replacing the Top Position Optical Switch.....................................6-10
6.3.4 Replacing the Plate Support Home Position Optical Switch ..........6-11
7. Electronic System
7.1 Introduction ....................................................................................7-1
7.2 Interconnection Diagram ...............................................................7-2
7.3 Main Board......................................................................................7-3
7.3.1 Replacing the Main Board ................................................................7-4
7.3.2 Replacing the Battery .......................................................................7-5
7.4 Power Supply Board ......................................................................7-6
7.4.1 Replacing the Power Supply Board..................................................7-7
7.5 Keyboard and Display Unit............................................................7-8
7.5.1 Replacing the Keyboard or Display Unit...........................................7-9
7.6 Manifold Detect Board .................................................................7-11
7.6.1 Replacing the Manifold Detect Board.............................................7-11
7.7 Fill Verification Board ................................................................. 7-13
7.7.1 Replacing the Fill Verification Board.............................................. 7-13
7.8 Optical Switches.......................................................................... 7-15
8. Serial Interface
8.1 Hardware Specifications............................................................... 8-1
8.1.1 Pin Designation ............................................................................... 8-1
8.1.2 RS-232-C Interface Lines ................................................................ 8-2
8.1.3 Synchronization and Data Format ................................................... 8-3
8.1.4 Daisy Chaining Instruments............................................................. 8-3
9. Description of Options
9.1 Instrument Options ....................................................................... 9-1
9.2 Liquid Detection Option ................................................................ 9-3
9.3 Barcode Reader Option ................................................................ 9-4
9.4 Printer Option ................................................................................ 9-5
9.5 Manifold Detection Option ............................................................ 9-6
9.6 Fill Verification Option .................................................................. 9-8
9.7 Aerosol Pump Option.................................................................... 9-8
9.8 Activating the Options .................................................................. 9-8
10. Maintenance
10.1 Cleaning Maintenance................................................................. 10-1
10.1.1 Cleaning the Manifold.................................................................... 10-1
10.1.2 Exchanging the Manifold ............................................................... 10-3
10.1.3 Exchanging the Plate Support ....................................................... 10-5
10.1.4 Instrument Disinfection .................................................................. 10-6
10.2 Preventive Maintenance Plan ..................................................... 10-8
10.2.1 Daily............................................................................................... 10-8
10.2.2 Weekly........................................................................................... 10-8
10.2.3 Every Six Months........................................................................... 10-8
10.2.4 Yearly (Service Technician Required) ........................................... 10-8
10.3 Service Tools ............................................................................... 10-9
10.3.1 Adjustment Plate (Reference Plate) .............................................. 10-9
10.4 Electrostatic Discharge Information ........................................ 10-10
10.4.1 Generating Static......................................................................... 10-10
10.4.2 Preventing Electrostatic Damage to Equipment .......................... 10-11
10.4.3 Personal Grounding Methods ...................................................... 10-11
11. Trouble Shooting

11.1 Introduction.................................................................................. 11-1
12. Instrument Specifications

12.1 Introduction.................................................................................. 12-1
Index
1. General
1. General
1.1 Overview
This is a technical manual for servicing the Columbus Washer.
The instrument is a microprocessor controlled washer, designed to wash
microplates.
The instrument can be fitted with either an 8, 12 or 16 way manifold or a 384 Well
Manifold.
The manifolds are fitted with one aspirating and one dispensing needle per well.
The instrument can store up to nine software user-defined plate types including
dispensing and aspirating positions as well as the shape of the plate.
The dispensing speed can be selected from five set speeds and dropwise.
These features allow the instrument to be used for all washing applications
including Cell Washing applications.
The instrument should only be opened by authorized personnel. Before the

instrument is opened the mains power cable must be disconnected from
the mains power socket.
Ensure that the instrument has been disinfected before it is serviced.
2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 1-1
1. General
1.2 Instrument Description

The diagram below shows the main components of the instrument:
Manifold Arm
Manifold
POWER
Power ON LED
Keyboard and
Display Unit
Waste Sink
Plate Support
Guide Bar
1.2.1 Rear Panel Connections

The instrument has the following connections on the back panel.
Mains Power
Socket
Fuse
Compartment
ON/OFF Inlets for channels 1 - 4

Switch
Hand held Bottle Holder with Printer Connector Aerosol Waste

Barcode Liquid Sensor for or Tubing Tubing
Reader each bottle Serial RS 232 for (option) (red)
Remote Control
1. General
(Instrument with Pinch Valves)
Channel 1 Channel 2 Channel 3 Channel 4

Inlet Inlet Inlet Inlet
Mains Power
Socket
Fuse Prime
Compartment valve
ON/OFF
Switch

Reader each bottle Serial RS 232 for (yellow) (red)
Remote Control
All connected devices must be proved and listed with regulations

EN 60950, UL 1950 or CSA C22.2 No. 950 for Data Processing Devices
1. General
1.2.2 Connection Diagram for Columbus (1 Liquid Channel)*
Hand held
Barcode
Reader (option)
Prime
valve
Printer (option)
or Marked
red
Marked
Inlet
green Rinse filter
solution
grey red
Waste
Wash green Bottle
Liquid
Computer for
Remote Control Bottle Holder with liquid
sensor for each bottle
* Some of the instruments may include the prime valve.
1. General
1.2.3 Connection Diagram for Columbus (4 Liquid Channels)*
Hand held Barcode

Reader (option)
Marked
Printer (option) red
Marked
yellow
Marked
purple
Marked
blue
or
Marked green
Marked grey
None Return Aerosol

Marked Valve Filter
Computer for blue
Water
Remote Control Seperator
Inlet
filter Inlet
filter
Waste Foam
Wash Rinse Bottle Wash Wash
Bottle
Liquid 1 solution Liquid 3 Liquid 2
grey red yellow purple
Bottle Holders with liquid

sensor for each bottle green blue
1. General
1.2.4 Keyboard and Display Unit

The Washer is operated via a keyboard and a control panel consisting of a two
row, sixteen digit Liquid Crystal Display (LCD) unit.
The top row of the display unit is used for messages and the bottom row for the
key functions or options.
A Light Emitting Diode (LED) in the center of the letter "O" of the word "POWER"
indicates when the Washer is switched ON.
Key 1 Key 2 Key 3 Key 4

POWER
Power
ON LED
1. General
1.3 Interconnection Diagram

The diagram below shows how the various parts of the instrument are
interconnected.
Dispensing Pump
Fill Verify and
Aspirating Pump Plate Mismatch
Board
Solenoid 4
Manifold Typeand
Aerosol Pump Plate Transport Manifold Arm Plate MismatchBoard Solenoid 3
Motor Motor
Solenoid 2
Plate Home Position
Optical Switch
Home Position Solenoid 1
Optical Switch
Dispense Liquid
Prime Valve Solenoid Detection
System
Orbital Shaking
Motor *
Printer / Serial
Connector
EEPROM
CPU
Barcode Reader
Keyboard /
Display Unit
Power Supply Board
Fuses
Mains Input
ON/OFF Switch
* The Orbital Shaking option is not used.
2.1 Unpacking and Inspection
The instrument is shipped in one carton, which contains:
• Instrument
• Accessory package which contains:
Spare manifold cover with two screws and Allen key
Operating manual
Mains power cable
Cleaning needles
2 spare fuses
Solution tube
3 spare 0 – ring seals for manifold
• Bottle set containing:
3 x 2.5 l bottles for the standard instrument
Liquid Sensor System Accessory package

(optional) and operating manual
Bottles Instrument
2.2 Unpacking Procedure

1. Visually inspect the container for damage before it is opened.
Report any damage immediately.
2. To place the instrument, select a location that is flat, level, vibration free,
away from direct sunlight, and free from dust, solvents and acid vapors.
Allow 10 cm distance between the back of the instrument and the wall or any
other equipment.
3. Place the carton in an upright position and open it.
4. Lift the instrument out of the carton and place it in the selected location.
5. Visually inspect the instrument for loose, bent or broken parts.
Report any damage immediately.
6. Compare the serial number on the rear panel of the instrument with the
serial number on the packing slip.
Report any discrepancy immediately.
7. Check the instrument accessories against the packing list.
8. Save packing materials for future use.
2.3 Power Requirements

The instrument is designed to operate at either 100 - 120 volt or 220 - 240 volt.
No voltage setting is required as the washer automatically senses the supplied
voltage.
The washer requires 2 x T 1.6 A / 250 V (slow blow) fuses.
WARNING
TO PREVENT THE RISK OF FIRE, THE MAINS FUSES SHOULD ONLY BE
REPLACED WITH THE SAME TYPE AND RATING OF FUSE.
2.4 Installation Procedure

Before the instrument is installed and switched on, it should be left to stand
for at least three hours, so there is no possibility of condensation causing a
short circuit.
2.4.1 Installing the Instrument

• Unpack the instrument and check the contents.
See 2.2 Unpacking Procedure.
• Connect the solution tubes to the inlet connectors.
See 2.4.3 Connecting the Solution Tubes.
• Setup possible instrument options. See page 2 – 5 and 2 – 6.
• Connect instrument to the power supply. See 2.4.7 Power Connections.
• Define the plate parameters in the Adjust menu. See chapter 3.
• Program the wash procedure. See chapter 3.
2.4.2 Connecting the Waste Tubing

Turn the instrument onto the right hand side.
Slide the waste tube through the hole in the back panel.
Aerosol Tube Hole Waste Tube Hole

(optional)
Connect the waste tube onto the top connector of the aspirating pump.
Standard Instrument
Waste Bottle
Dispensing
Pump
Waste Tube
Aspirating Tube to
Aspirating Pump
Solution Tubes to Manifold
Turn the instrument back to the normal position.

Place the other end of the waste tubing into the waste bottle.
Connect the aerosol tube onto the aerosol pump if this option is fitted. This is not
necessary for the standard instrument.
Instrument with Aerosol Protection Option
Waste Bottle
Dispensing Pump
Aerosol
Aerosol Bottle Pump
Aerosol Tube to Manifold
Waste Tube
Aspirating Tube to Manifold
Aspirating Pump
Turn the instrument back to the normal position.

Place the other end of the aerosol tubing into the aerosol bottle.
2.4.3 Connecting the Solution Tubes

The solution tubes are connected onto the inlet connectors on the back panel of
the instrument. The instrument is supplied with four meters of tubing that can be
cut to the required length (maximum 1 meter).
Inlets for channels 1 - 4
Aerosol Tube* Waste Tube

(optional) red
2.4.4 Fitting and Connecting the Liquid Sensors (Optional)

Connect the detector unit cable into the appropriate socket in the back panel of
the instrument. The liquid sensor system can be extended to control up to four
different liquid bottles and one waste bottle.
rinse waste
grey red
liquid 1
green
Liquid Sensor
Fix the tube support in the bottle holder as shown below.
Tube support
Insert the tubes and connect the bottle holder to the instrument.
2.4.5 Connecting the Barcode Reader (Optional)

Before the barcode reader can be connected to the instrument, the supplied
barcode chips must be added to the main board. For more details contact your
service technician.
Hand Held Barcode Liquid Sensor

Reader
2.4.6 Connecting the Printer (Optional)

Before the printer can be connected to the instrument, the supplied chip must be
added to the main board. For more details contact your service technician.
Connect the printer to the instrument using the supplied cable.
Connect the power supply cable to the printer.
Power Cable
ON /OFF
Switch
POWER OFF 1 2
ON
Connection Cable
BATT. LOW
PAPER
POWER FEED
2.4.7 Power Connections
Before the instrument is installed and switched on, it should be left to

stand for at least three hours, so there is no possibility of condensation
causing a short circuit.
Ensure that the on / off switch in the back panel of the instrument is in the off
position.
Insert the mains power cable into the mains power socket on the rear of the
instrument.
3. Firmware and Software

Description
3.1 Instrument Software Features
The instrument has the following software features:
• 30 user definable washing programs, permanently stored under the
numbers 1 to 30.
• Up to 60 processing steps per program, each processing step can be
identical or different from the previous step.
• 3 washing modes, normal washing, bottom washing and overflow washing.
Set by the Plate Type parameters.
• Soak time. selectable from 1 to 999 seconds.
• Software selectable dispensing speeds
• Software selectable aspirating speeds
• Software selectable bottom position, for aspiration contact to the bottom
of the wells.
• 4 rinse modes, the different rinse modes can be used to select how the
instrument is rinsed before it is left to stand or switched OFF.
• Automatic microplate centering, the microplate is automatically centered
before the start of the washing program.
• Programmable strip selection, the program can be defined so that the
strips to be washed can be selected before starting the washing procedure.
3.2 Onboard Software Menus

The instrument can be used in the following onboard software menus.
3.2.1 Run
Selection of the run procedure to perform the selected program.
3.2.2 Prime
Before the instrument can be used, all dispensing channels must be primed
with the required solution.
Ensure that the bottle of priming solution is always full at the beginning of
the priming procedure and check that the inlet filters in the liquid tubes are
clear.
Please ensure that the dispensing and aspiration pumps are not run for
longer than a few minutes without liquid or they will be damaged.
The priming procedure is performed using the following procedure:
Run XX yyyyyyyy
- + other yes
Prime Ch1 Select required channel to be primed

- + other yes
Prime Solution ? Required solution in bottle and inlet

exit yes
tube inserted into solution
Waste Bottle OK? Waste bottle empty

exit yes
Prime ChX Y sec Priming procedure being performed

Stop
Priming Procedure
performed
Run XX yyyyyyyy Instrument ready to wash microplate

- + other yes
Caution
Do not use the instrument to aspirate or dispense any acidic solutions as
this could damage the instrument.
3.2.3 Rinse
There are four Rinse modes to flush the liquid system and prevent needle
blockages. They should be carried out when the instrument is left to stand or is
switched off at the end of working.
The Rinse modes are:
Rinse Soak
Rinse Day
Rinse Night
Rinse Auto
Rinse: Soak
This procedure is used if the instrument is to be left to stand for a short time.
(1/2 hr. or less).
With this procedure you can use either distilled water or wash solution.
Rinse: Soak Select and confirm Soak Rinse procedure

- + other yes
Soak: CH1 Select required channel (1 - 4)

- + exit yes
Rinse Solution ? Confirm that the rinsing solution

exit yes and the tube are in the bottle
Waste Bottle OK? Confirm waste bottle is empty

exit yes
The waste sink is filled with solution
and the manifold is lowered into it
Soaking Manifold left in solution until Stop key pressed

Stop
Run XX:yyyyyyy Standby mode

- + other yes Washing procedure can be performed
Rinse: Day
This procedure is used to rinse the wash solution system if the instrument is to be
left standing for a longer time. Alternatively it can be used to rinse the wash
solution system after the instrument has been left for a long time.
With this procedure you can use either distilled water or wash solution.
If the instrument is left to stand for 1 hr to 1/2 day, wash solution can be used, for
longer times use distilled water.
Rinse: Day
- + other yes
Select and confirm Day Rinse procedure
Day: CH1 Select required channel (1 - 4)

- + exit yes
Rinse Solution ? Confirm rinsing solution and tube in bottle

exit yes

exit yes
Rinse ChX YY sec Wash system rinsed for set rinse time
Stop
The waste sink is filled with solution and

the manifold is lowered into it
Soaking Manifold left in solution until

Stop Stop key pressed

- + other yes
Washing Procedure can be performed
Rinse: Night
This procedure is used to thoroughly rinse the wash solution system and leave
the manifold soaking in the waste sink full of solution at the end of working. This
procedure requires distilled water.
Rinse: Night Select and confirm Night Rinse procedure

- + other yes
Night: CH1 Select required channel (1 - 4 or ALL)

- + exit yes
Rinse Solution ? Confirm rinsing solution and tube in bottle

exit yes

exit yes
Stop
The waste sink is filled with the distilled

water and the manifold is lowered into it
Soaking xx sec Manifold left in solution for set soak time (1 min)
Stop
Stop
The waste sink is filled with the distilled water

and the manifold is lowered into it.
Soaking Manifold left in solution until Stop key pressed

Stop

- + other yes Washing procedure can be performed
Rinse: Auto
This procedure is the same as the Rinse: Day procedure, except that the rinsing
procedure is automatically repeated after a set repeat time. The repeat time can
be set in the Adjust menu between 1 and 24 hrs.
3.2.4 Program
Selection of the programming procedure, to define the required program.
For more information about the programming procedure, see chapter 4 in

the Columbus Operating Manual.
3.2.5 Clear
This submenu is used to clear a program from the instrument's memory. If the
program is locked it can not be cleared and the following error message is
displayed:
Program locked
yes
Clear XX:yyyyyyy Select required program to be

- + other yes cleared from instrument's memory
Clear XX:yyyyyyy Confirm that selected program is

no yes to be cleared
Clear XX:yyyyyyy Select next program to be cleared or

- + other yes required option in Mode Selection menu
The function Clear ALL is available within the Clear Program option. This
function enables you to clear all of the programs (excluding those that are
locked!). The Clear ALL function is found before program 1 and after program
30. Use the cursor keys to skip through the programs and once Clear ALL is
displayed press yes and all of the programs (with the exception of those that are
locked) will be cleared.
This option is not to be confused with the Clear ALL function in the Service
menu.
3.2.6 Show
Procedure to show the parameters for a program.
For more information about the Show procedure, see Columbus Operating
Manual.
3.2.7 Adjust
The Adjust menu has the following options:
Plate Type Adjustment procedure for setting the parameters for up

to nine different types of microplates
Prime Time Adjust the time that the Priming procedure is performed
for.
Rinse Time Adjust the time that the Rinsing procedure is performed
for.
Autorinse Adjust the time between the Autorinse cycles.
Kit Select This option is not available in this instrument.
Date Set the date
Time Set the time
Sel. Manifold Set the manifold that is fitted to the instrument
Adj. Liq. Sensor The switch thresholds for the wash, rinse and waste
liquid can be defined.
Exit Adjust Exit from the Adjust menu.
Entering the Adjust Menu

When the instrument is in the standby mode and the following message is
displayed:
Run XX yyyyyyyy
- + other yes
To select the Adjust menu, press other until the following message is displayed:
Adjust Menu
other yes
Select yes to enter the Adjust menu and then the various options in the Adjust
menu can now be selected using other.
When the required option is displayed, press yes to enter the parameter setting
procedure.
Adjust Menu Confirm selection of Adjust Menu

other yes
Platetype: 1 Plate Type definition menu

- + other yes Use '-' and '+' keys to select required platetype
Plate Type definition procedure
Priming Time setting

Primetime:10 sec
- + other yes Use '-' and '+' keys to select required priming time
(5 - 99 sec.)*
Rinsetime: 10sec Rinse Time setting

- + other yes Use '-' and '+' keys to select required rinsing time
(5 - 99 sec.)*
AutoRinse: 1 h Automatic Rinsing Time setting

- + other yes Use '-' and '+' keys to select required time between
Automatic Rinsing procedures (1 - 24 hrs.)
Kit Select: Off This option is not available in this instrument

- + other yes
Date Setting
Date: ddmmmyyyy Use 'd' key to change day setting
d m other y
Use 'm' key to chang month setting
Use 'y' key to change year setting
Time: hh:mm xx
Time Setting
h m other form Use 'h' key to change hour setting
Use 'm' key to chang minute setting
Use 'Form' key to change from 12 to 24 hrs format
Sel.Manifold: xx Manifold setting

- + other yes Use '-' and '+' keys to select fitted manifold. (8, 12, 16)
If Manifold detect option fitted, this setting is used
as the default when programming
Adj.Liq.Sens.:
other yes
Liquid Detection Sensor setting
Exit Adjust Exit Adjust Menu

other yes
* Instruments that have the prime valve have a priming time of 8 - 99 seconds.
Adjust Plate Type

This option is used to set the following parameters for the different types of
microplates. The default is a 96 well Greiner microplate.
The following parameters are set:
Parameters Options
Plate type number 1-9
Bottom Form Set whether the microplate has flat or round
bottom wells.
Dispensing Position Set the position in the wells where the solution
will be dispensed.
Aspirate Position Set the position in the wells where the solution
will be aspirated.
Aspirate Position 2 Set the position in the wells for the crosswise
aspiration procedure. (flat bottom wells only)
Bottom Position Set the position of the aspirating needles for the
bottom of the well.
Bottom Wash Position Set the position of the aspirating needles for the
bottom washing procedure.
Overflow Wash Set the position of the aspirating needles for the
Position overflow washing procedure.
Before starting the adjustment procedure, insert the microplate to be

adapted into the plate support.
Round Bottom Plates

Adjust Menu Confirm selection of Adjust menu
other yes
Plate: X
Plate Type definition menu
- + other yes Use '-' and '+' keys to select required platetype (1 - 9)
Enter the required name for the plate

Plate: X (up to 8 characters). 1,2,3,4,5,6,7,8,9,0 & A-Z
890 , , ABCDE To enter a name use the ‘<’ and ‘>’ keys to place a character
between the commas. Confirm the selection with the ‘↵’ key.
To delete the last character use the ‘ ’ sign.
When the name is complete, confirm with the ‘↵’ key.
Bottomform Round
Plate Bottom Form setting
- + exit yes Use '-' and '+' keys to select required bottom form
(flat or round)
Disp.Pos XXX Set Dispensing position

- + exit yes Use '-' and '+' keys to move the plate support so
that the dispensing needles are in the correct position
Asp.Pos XXX Set Aspirating position

that the aspirating needles are in the correct position
Set Bottom Aspirating position

Asp.Pos Botm XXX Use '-' and '+' keys to move the manifold so that the
- + exit yes
aspirating needles are in the correct position for
aspirating the bottom of the wells
Washpos Botm XXX

Set Bottom Washing position
- + exit yes Use '-' and '+' keys to move the manifold so that the
the Bottom Washing procedure
Washpos Ovfl XXX Set Overflow Washing position

the Overflow Washing procedure
Platetype: X Select plate type to be defined

- + other yes
Flat Bottom Plates

Adjust Menu Confirm selection of Adjust menu
other yes
Plate: X Plate Type definition menu

- + other yes Use '-' and '+' keys to select required platetype (1 - 9)
Enter the required name for the plate

Plate: X (up to 8 characters). 1,2,3,4,5,6,7,8,9,0 & A-Z
890 , , ABCDE To enter a name use the ‘<’ and ‘>’ keys to place a character
between the commas. Confirm the selection with the ‘↵’ key.
To delete the last character use the ‘ ’ sign.
When the name is complete, confirm with the ‘↵’ key.
Bottomform Flat Plate Bottom Form setting

- + exit yes Use '-' and '+' keys to select required bottom form
(flat or round)
Disp.Pos XXX Set Dispensing position

that the dispensing needles are in the correct position
Asp.Pos 1 XXX Set Aspirating position 1

Asp.Pos 2 XXX Set Aspirating position 2

Set Bottom Aspirating position

Asp.Pos Botm XXX
aspirating the bottom of the wells
Washpos Botm XXX

Set Bottom Washing position
the Bottom Washing procedure
Washpos Ovfl XXX Set Overflow Washing position

the Overflow Washing procedure
Platetype: X Select Plate type to be defined

- + other yes
To enter the Plate type option, press other until the following message is
displayed:
Plate: XXXXX
- + other yes
Where X is the displayed plate type number and plate name.

Use the - and + keys to select the required plate type number that is to be
defined. (1 - 9).
Pressing - decreases the displayed plate type.
Pressing + increases the displayed plate type.
When the required plate type is displayed press yes and the following message is
displayed:
Bottomform Flat
- + exit yes
Use the - and + keys to select the required bottom form of the wells. (flat or
round).
When the required bottom form is displayed press yes to confirm the selection.
Flat Bottom Wells
The following procedure is used when a microplate with flat bottom wells is to be
defined.
After yes has been pressed to confirm the selection of flat bottom wells, the
instrument initializes the motors and moves the microplate under the manifold.
When the microplate is under the manifold, the following message is displayed:
Disp-Pos: XXX
+ exit yes
Where XXX is the displayed number of steps.

Use the - and + keys to move the microplate until the dispensing needles are in
the correct position.
Pressing the - and + keys moves the microplate forwards and backwards so that
the dispensing needles are positioned further back/forward in the wells.
When the dispensing needles are in the correct position, press yes and the
following message is displayed:
Asp.Pos 1 XXX
- + exit yes
Use the - and + keys to move the microplate until the aspirating needles are in
the correct position for aspirating the side of the well.
the aspirating needles are positioned further back/forward in the wells.
When the aspirating needles are in the correct position, press yes and the
Asp.Pos 2 XXX
- + exit yes
the correct position for aspirating the side of the well. (crosswise aspiration)
When the aspirating needles are in the correct position press yes and the
Asp.Pos Botm XXX

- + exit yes
Use the - and + keys to move the manifold until the aspirating needles are in the
correct position for aspirating the bottom of the well.
Pressing the - and + keys move the manifold up and down so that the aspirating
needles are positioned higher/lower in the wells.
Washpos Botm XXX

- + exit yes
correct position for aspirating the solution in the bottom washing position.
Pressing the - and + keys moves the manifold up and down so that the aspirating
Washpos Ovfl XXX

- + exit yes
correct position for aspirating the solution in the overflow washing position.
Pressing the - and + keys move the manifold up and down so that the aspirating
Platetype: X
- + other yes
The parameters for the next plate type can be defined or press other for the next
option in the Adjust menu.
Round Bottom Wells

The following procedure is used when a microplate with round bottom wells is to
be defined.
After yes has been pressed to confirm the selection of round bottom wells, the
instrument initializes the motors and moves the microplate under the manifold.
When the microplate is under the manifold, the following message is displayed:
Disp.Pos XXX
- + exit yes

Use the - and + keys to move the microplate until the dispensing needles are in
the correct position.
the dispensing needles are positioned further back/forward in the wells.
When the dispensing needles are in the correct position, press yes and the
Asp.Pos XXX
- + exit yes
the correct position for aspirating the middle of the well.
Asp.Pos Botm XXX

- + exit yes
correct position for aspirating the bottom of the well.
Washpos Botm XXX

- + exit yes

correct position for aspirating the solution in the bottom washing position.
Washpos Ovfl XXX

- + exit yes
correct position for aspirating the solution in the overflow washing position.
Platetype: X
- + exit yes
The parameters for the next plate type can be defined or press other for the next
option in the Adjust menu.
Adjust Prime Time

This option is used to set the time that the Prime procedure is performed for.
Primetime:10 sec Use '-' and '+' keys to select required priming
+ - other yes time (5- 99 seconds)*
Adjust menu
other yes
Rinsetime:10 sec
- + other yes
To enter the Prime Time option, press other until the following message is
displayed:
Primetime:10 sec
- + other yes
Use the - and + keys select the required priming time in seconds. (5 - 99 sec)*
When the required prime time is displayed, either press other to proceed to the
next option or press yes to exit from the Adjust menu.
Adjust Rinse Time

This option is used to set the time that the rinse procedure is performed for.
Rinsetime:10 sec Use '-' and '+' keys to select required rinsing
+ - other yes time (5 - 99 seconds)*
Adjust menu
other yes
Autorinse:1 h
- + other yes
To enter the Rinse Time option, press other until the following message is
displayed:
Rinsetime:10 sec
- + exit yes
Use the - and + keys select the required rinse time in seconds. (5 - 99 sec)*
When the required rinse time is displayed, either press other to proceed to the
next option or press yes to exit from the Adjust menu.
* Instruments that have the prime valve have a priming time of 8 - 99 seconds.
Adjust Auto Rinse

This option is used to set the time between the rinse procedures when the
Autorinse mode is selected.
AutoRinse: 1h Use '-' and '+' keys to select required Auto

+ - other yes rinsing time (1 - 24 hours)
Adjust menu
other yes
Kit Select: Off

- + other yes
To enter the AutoRinse Time option, press other until the following message is
displayed:
AutoRinse: 1 h
- + exit yes
Use the - and + keys select the required time in hours between each automatic
rinse procedure. (1 - 24 hours)
When the required time is displayed, either press other to proceed to the next
option or press yes to exit from the Adjust menu.
Kit Select
This option is not available in the instrument.
Date
The setting for the date can only be altered if the Printer option is fitted.
Date: dd mmm yyyy Use the 'd' key (1st key) to set the required Day
d m other y Use the 'm' key (2nd key) to set the required Month
Use the 'y' key (4th key) to set the required Year
Time: hh:mm:ssXX
h m other form
To enter the Date option, press other until the following message is displayed:
Date:dd mmm yyyy

d m other y
Where dd mmm yyyy is the displayed date.
Use the d, m and y to set the required date.

Pressing d increases the displayed day setting
Pressing m increases the displayed month setting
Pressing y increases the displayed year setting
When the required date is displayed, press other to confirm the setting and the
Time: hh:mm:ssXX
h m other form
Time
The setting for the time can only be altered if the Printer option is fitted.
Time: hh:mm:ssXX Use the 'h' key (1st key) to set the required Hour
h m other form Use the 'm' key (2nd key) to set the required Minute
Use the 'Form' key (4th key) to set the required form
AM/PM or 24 hr.
Sel.Manifold: XX
- + other yes
To enter the Time option, press other until the following message is displayed:
Time: hh:mm:ssXX
h m other form
Where hh:mm:ss is the displayed time and XX is the time form (AM/PM).
Use the h, m and form to set the required time and format.
Pressing h increases the displayed hour setting
Pressing m increases the displayed minute setting
Pressing form changes the time format (a.m./p.m. or 24 hr.)
When the required time and format is displayed, press other to confirm the
setting and the following message is displayed:
Sel.Manifold: XX
- + other yes
Select Manifold
This option is used to set the type of manifold that is fitted.
This setting is used if neither the manifold detect option nor the fill
verification option are fitted.
Sel.Manifold: XX Use the '-' key (1st key) and '+' (2nd key) to set
- + other yes the setting for the manifold (8, 12 or 16 way)
Press 'yes' key (4th key) to confirm and save setting
Press 'other' key (3rd key) to move to next option
without saving setting
Exit Adjust
other yes
To enter the Select Manifold option, press other until the following message is
displayed:
Sel.Manifold: XX
- + other yes
Use the - and + keys to set the required manifold. (8, 12 or 16 way)
Press other to proceed to the next option without saving any changes in the
setting.
Press yes to confirm the setting and proceed to the next option.
When other or yes has been pressed, the following message is displayed:
Adjust Liq. Sens.:

other yes
Adjust Liquid Sensors
Caution
This procedure must be performed when the instrument is installed.
Please prepare the bottles before performing the procedure below. The bottles
marked green (wash solution) and gray (rinse solution) should be filled with
600ml of water and the bottle marked red (waste) should be filled with 2000 ml of
water. Place all three bottles on the corresponding colored bottle holders. Go to
the Adjust menu and select Adj. Liq. Sens. as described below:
Adj. Liq. Sens.: Press yes to start the adjustment procedure

other yes
Using the - and + keys select the liquid sensor to
be calibrated.
1 = Green (wash solution)
Liq. Sens. 1 2 = Gray (rinse solution)
- + exit yes W = Red (waste)
Press yes to continue the adjustment procedure.
Check that the adjustment of the magnet screw has

a blank value of between 5 and 20. See photo1.
Empty 1 XX 1 XX = displayed sensor value (does not directly
exit yes correlate to the volume of liquid in the bottles).
1 = values above limit
Press yes to confirm the limit.
Empty 1 XX 0 XX = displayed value 0 = values below limit

exit yes Press exit to continue the adjustment of other sensors.
Use the cursor keys to repeat this procedure

for the grey (rinse solution) and red (waste)
bottles. See also below for more details.
Photo 1
Check that the adjustment of the magnet

screw has a blank value of between
5 and 20
If it was necessary to readjust the
settings, secure the screws with
adhesive.
Liquid Sensors 1 – 2 (3 – 4)
When a liquid sensor (1 - 2) has been selected, the following message is
displayed:
Empty S XX Y
exit yes
Where S = the sensor number, XX is the displayed value and Y = limit

(0 = values below limit, 1 = values above limit)
Adjust the volume of solution in the bottle to the lowest volume that can be used
before starting the procedure.
When the minimal volume is in the bottle, press yes to confirm the value
(volume).
Green bottle (wash solution) = 600 ml
Gray bottle (rinse solution) = 600 ml
When the sensor detects a volume less than this entered volume, it will display
an error message either before starting the test procedure or after the procedure
has been completed.
When the value has been set, press exit and the following message is displayed:
Liquid Sens. 1
- + exit yes
Use the < and > keys to set the next required sensor (1 – 2 or W)
Waste Sensor
When the waste sensor has been selected, the following message is displayed:
Full W XX Y
exit yes
Where W = Waste sensor, XXX is the displayed value and Y = limit

(0 = values below limit, 1 = values above limit)
Adjust the volume of solution in the waste bottle to the highest volume that can be
used before starting the procedure.
When the maximum volume is in the bottle, press yes to confirm the value
(volume).
Red bottle (waste) = 2000 ml
When the sensor detects a volume greater than this entered volume, it will
display an error message either before starting the test procedure or after the
procedure has been completed.
When all the sensors have been set, press exit and the following message is
displayed:
Adj.Liq.Sens.:
other yes
Press other to proceed to the next option.
Exit Adjust
This option is used to exit from the Adjust menu.
Exit Adjust Select if the Adjust Menu is to be exited or not

other yes
Run X:yyyyyyy
- + other yes
Platetype: 1
- + other yes
When the following message is displayed:
Exit Adjust
other yes
Select if the Adjust menu is to be exited or not.

Remain in Adjust Menu
Press other to remain in the Adjust menu and the following message is
displayed:
Platetype: 1
- + other yes
Select the required option in the Adjust menu.

Exit Adjust Menu
Press yes and the following message is displayed:
Run X:yyyyyyy
- + other yes
The instrument is now in the Standby mode and can be used to wash a
microplate.
3.2.8 Service Menu

The Service submenu has the following options:
Clear All ? Procedure to clear all the defined programs and

instrument settings from the memory.
Prog Access Procedure to select if access to the programming
procedure is allowed.
Adj. Access Procedure to select if access to the Adjust menu is
allowed.
Header Procedure for defining the start-up header for the
instrument.
Channels Procedure for setting the number of dispensing
channels to be used.
Skip Messages Procedure to set if the reminder messages are to be
displayed.
Duration Test Procedure for life testing the instrument.
Function Test Procedure for testing various instrument component.
Select Options Procedure for defining fitted instrument options.
Adj. Dispens. Pump Procedure for adjusting the dispensing pump.
Adj. Prime Pos. Procedure for adjusting the position of the plate support
for the Prime / Rinse procedures. (Important adjustment
with 16 way manifold)
Adj. Plate Ref. Procedure for adjusting the position of the manifold
needles.
Service
other yes
Enter required code '121418' (for firmware

Code: V4.20 and higher)
1 2 1 4 1 8
(Enter ‘1248’ for firmware V4.01 up to but not
including V4.20)
Clear All ? Clear All programs and settings from memory

yes exit no Attention: This deletes parameter settings
which require special tools to be redefined.
Prog Access: On Select access to programming procedure

- + exit yes If access is off the programme menu is omitted
from the Mode Selection menu.
Adj. Access: On Select access to Adjust menu

- + exit yes If access is off the Adjust menu is omitted
from the Mode Selection menu.
Header: Set startup header for instrument

Set exit yes
Channels: X Set number of dispensing channels fitted (1 - 4)

- + exit yes
Skip Messages:
yes exit no Select if warning message are to be skipped
Duration Test
yes exit no Life testing procedure
Function Test Procedure for testing the various

yes exit no
instrument componants
Select Options Procedure for defining fitted instrument options

yes exit no
Adj Dispens Pump Procedure for adjusting dispensing pump

yes exit no
Adj.Prime Pos. Procedure for adjusting the position of the

yes exit no plate support for the Prime / Rinse procedures.
(Important adjustment with 16 way manifold)
Service End
yes no Exit Service menu
Standby Mode
Entering the Service Menu

The Service menu is entered using the following procedure:
When the instrument has been switched on, it is in the standby mode and
displays the message
Run XX yyyyyyyy
- + other yes
Where XX is the displayed program number and yyyyyyyy the program name.
To select the Service menu, press other until the following message is displayed:
Service
other yes
Press yes to enter the Service menu and the following message is displayed:
Code:
1 2 1 4 1 8
The Service submenu can only be entered by entering the required code.
Enter required code '121418' (for firmware V4.20 and higher)
(Enter ‘1248’ for firmware V4.01 up to but not including V4.20)
When the code has been correctly entered the first option of the Service menu is
displayed:
Clear ALL ?
yes exit no
Clear ALL
This option is used to clear ALL the defined programs and instrument settings
from the instrument's memory.
This deletes parameter settings, which require special tools to be

redefined.
Service
other yes
Clear All ? Clear All defined programs and settings

yes exit no
Are you sure ? Confirm that All defined programs and

exit yes settings are to be cleared
Initializing Instrument re-initialized

please wait
Run 1: Instrument in Standby Mode

- + other yes
Prog Access: On Select if access to the programming procedure

- + exit yes
is to be allowed or not.
Clear ALL ?
yes exit no
Select if all the programs and settings are to be cleared or not.

Press no if all the programs are not to be cleared, the instrument then proceeds
to the next option.
Press exit to exit from the Service menu.
Press yes to clear all the programs from the instrument's memory and the
Are you sure ?

exit yes
This message is displayed as a control to ensure that the programs and

settings are not accidentally cleared.
Press exit to return to the previous message.
Press yes to confirm that all the programs are to be cleared.
After yes has been pressed, all the programs and settings are cleared from the
instrument's memory, the instrument is re-initialized and the following message is
displayed:
Run 1:
- + other yes
The instrument is now in the Standby mode.

Re-program the instrument for the required programs and settings.
Instrument options, dispensing pump calibration, priming position.
The following options must be re-calibrated: the dispensing pump calibration, the
liquid sensor and bottle management system and the X and Z offset value using
adjustment plate.
The following data must be reprogrammed using the WinWash software (see
software manual): test parameters, options and plate types.
Programming Access
This option is used to select if access to the programming procedure is permitted.
Service
other yes
Prog Access: On Use '-' and '+' key to select if access to

- + exit yes programming procedure is permitted.
Prog Access: Off

- + exit yes
Adj. Access: On Use '-' and '+' key to select if access to

- + exit yes Adjust menu is permitted
Prog Access: On
- + exit yes
st nd
Use the 1 and 2 keys to select if access is permitted.
On = access permitted, Off = access NOT permitted
When the required setting is displayed, press yes and the following message is
displayed:
Adj. Access: On
- + exit yes
When the access is not permitted, the menu point Programme is omitted from the
Mode Selection menu.
Adjust Access
This option is used to select if access to the Adjust menu is permitted.
Service
other yes
Adj. Access: On Use '-' and '+' key to select if access to

- + exit yes Adjust Menu is permitted.
Adj. Access: Off

- + exit yes
Header: Procedure to define startup header

Set exit yes
Adj. Access: On
- + exit yes
st nd
Use the 1 and 2 keys to select if access is allowed or not.
On = Access allowed, Off = Access NOT allowed
When the required setting is displayed press yes and the following message is
displayed:
Header:
Set exit yes
When the access is not allowed, the Adjust menu is omitted from the Mode
Selection menu.
Header
This option is used to define the start-up header for the instrument.
Service
other yes
Header: Select if header is to be altered or not

Set exit yes
COLUMBUS Edit or enter the required header

890 ↵ ABCDE
Channels: X Select number of dispensing channels fitted

- + exit yes
Header:
Set exit yes
Press Set to enter the header setting procedure.

Press yes to confirm the displayed header and proceed to the next option.
When Set has been pressed, the following message is displayed:
COLUMBUS
890 ABCDE
In the bottom row of the display are shown the characters that can be selected for
the header. A maximum of sixteen characters can be used.
The possible characters for the header are:
1234567890ABCDEFGHIJKLMNOPQRSTUVWXYZ
There are two special characters ' ' and ' ' .
The character ' ' is used to clear the last entered character from the header.
The character ' ' is used to confirm that the completed header has been entered
and is used to exit from the header programming procedure.
st nd
To enter the required header, use the 1 and 2 keys to scroll the characters
across the display.
When the required character is displayed between the two highlighters in the
th
middle of the display, press the 4 key. The character is then entered into the top
line of the display.
After the selected character has been entered, the instrument returns to the start
position with the ' ' character between highlighters. For example:
WASHER
890 ABCDE
When the required header has been entered, the header programming procedure
th
is exited by pressing the 4 key when the ' ' character is between the two
highlighters.
The following message is displayed:
Channels: X
- + exit yes
Channels
This option is used to select the number of dispensing channels that are fitted to
the instrument.
Service
other yes
Channels: Use '-' and '+' keys to define the

- + exit yes of dispensing channels fitted (1 - 4)
Skip Select if warning messages are to be

yes exit no skipped or not.
Channels: X
- + exit yes
st nd
Use the 1 and 2 keys to select the number of channels that are fitted and that
can be used. (1 - 4)
When the required setting is displayed, press yes and the following message is
displayed:
Skip Messages:
yes exit no
Skip Messages
This option is used to select if the warning messages are to be displayed or not.
Skip Messages:
yes exit no
Channel primed? Select if this message is to be displayed

show exit skip Press 'show' key to display message
Press 'skip' key to omit message
Plate inserted ? Select if this message is to be displayed

Waste Bottle OK? Select if this message is to be displayed

Rinse Solution ? Select if this message is to be displayed

Prime Solution ? Select if this message is to be displayed

You should Rinse Select if this message is to be displayed

Skip Messages:
yes no
Service Duration Test

other yes yes exit no
Skip Messages:
yes exit no
Press no if the warning messages are not to be skipped. The instrument then
proceeds to the next option.
Press yes if the warning messages are to be skipped.
When yes is pressed, the following message is displayed:
Channel X primed?
show exit skip
Press show if this message is to be displayed.

Press skip if this message is not to be displayed.
When the key for the required selection has been pressed, the following message
is displayed:
Plate inserted ?
show exit skip
Press show or skip and the following message is displayed:
Waste Bottle OK?

show exit skip
Rinse Solution ?
show exit skip
Prime Solution ?
show exit skip
You Should Rinse

show exit skip
Skip Messages:
yes no
Press yes to exit from the Service menu.

Press no to proceed to the next option in the Service menu.
When no is pressed, the following message is displayed:
Duration Test
yes exit no
Duration Test
This option is used to select the life test procedure for the instrument.
Before starting the Duration Test procedure, place a microplate into the plate
support.
Service
other yes
Duration Test Duration Testing procedure

yes exit no
Function Test
yes exit no
C:XX h:mm:ss Instrument displays values from last

clr exit cont
Duration Test procedure
Press 'clr' to clear values
Press 'cont' to start procedure
Run XX yyyyyyyy
- + other yes Select required program
Nr.of Strips: XX
- + exit yes Select required number of strips
Channel X primed?
no yes Required channel primed
Priming
procedure
Plate inserted ?
exit yes Plate inserted
Waste Bottle OK? Is the waste bottle empty and

exit yes the waste tube connected?
Duration Test
procedure started
C: X Yxxxxx Instrument displays number of cycles

h:mm:ss performed, the type of step being performed
and the total time for the Duration Test.
Press 'Stop' key (3rd key)
to stop Duration Test
Run XX yyyyyyyy Instrument in Standby Mode

- + other yes
Duration Test
yes exit no
Press no if the life test procedure is not to be performed. The instrument then
proceeds to the next option and the following message is displayed:
Function Test
yes exit no
Press yes to enter the life test procedure and the following message is displayed:
C:XX h:mm:ss
clr exit cont
This message displays the values from the last Duration Test procedure C:XX =
number of cycles performed, h:mm:ss = time that the procedure was performed
for.
Press clr to clear the values.
Press exit to return to the previous message.
Press cont to start the Duration Test procedure.
When cont is pressed, the following message is displayed:
Run 1:XXXXXXX
- + other yes
Use the - and + keys to select the required test that is to be used for the Duration
Test procedure. Any defined test procedure can be used.
When the required test procedure is displayed, press yes to confirm the selection.
Nr of Strips: XX
- + exit yes
Use the - and + keys to select the required number of strips to be used (1 - 12).
When the required number of strips is displayed, press yes to confirm the
selection.
Channel Xprimed?
no yes
Where X is the displayed channel number.

This optional message can be displayed as a reminder to ensure that the
instrument has been primed with the correct solutions.
If the required channel has not been primed, press no and the instrument
automatically exits from the Run mode and enters the priming procedure.
If the required channel has been primed, press yes and the following message is
displayed:
Plate inserted ?
exit yes
This optional message can be displayed as a reminder to ensure that the

microplate has been inserted into the plate support.
When the microplate has been inserted into the plate support, press yes and the
Waste Bottle OK?

exit yes
This optional message can be displayed as a reminder to ensure that the waste
bottle is empty and that the waste tube is in the waste bottle.
This message is not displayed if Waste Full Sensor is fitted.
Press yes to confirm that the waste bottle is empty and the waste tube is inserted
into the waste bottle and the instrument then starts the Duration Test procedure.
While the instrument is performing the Duration Test procedure, messages are
displayed giving information about the procedure. For example:
C:XX Yxxxx
h:mm:ss
Where C:XX = number of cycles performed, Yxxxx = the type of program step
that is being performed and h:mm:ss = the time that the Duration Test has been
performed for.
The life test procedure is repeated until the Stop key is pressed.
To stop the life test procedure, press Stop and the instrument then returns to the
standby mode and the following message is displayed:
Run XX yyyyyyyy
- + other yes
If you wish to restart the Duration Test press the 4th key and the test will continue.
Where XX is the displayed program number and yyyyyyyy the program name.
Function Test
This option is used to check various instrument component functions.
Service
other yes
Function Test Function Testing procedure

yes exit no
Select Options
yes exit no
Motor Test
yes exit no
Motor testing procedure
Solenoid Test
yes exit no
Solenoid testing procedure
Sensor Test
yes exit no
Sensor testing procedure
Test Fill Verif.

yes exit no
Fill Verification testing procedure
Barcode Test
yes exit no
Barcode Reader testing procedure
Orbit.Shake:
yes exit no
Orbital Shaking testing procedure
(This option is not used.)
Liq.Sens.
yes exit no
Liquid Detection testing procedure
uC: 80517A
or uC: 80537
exit yes
exit yes
Function Test
yes exit no
Press yes to enter the Function Test procedure and the following message is
displayed:
Motor Test
yes exit no
Use no to select the Function Testing procedure that is to be performed.

The following function test can be performed:
Motor Test Test the various motors
Solenoid Test Test the various solenoid valves
Sensor Test Test the various sensors.
Test Fill Verif. Test Fill Verify option
Barcode Test Test Barcode Reader
Liq. Sens. Test the Liquid Sensor System
Motor Testing
This option is used to check that the various motors are functioning correctly.
Function Test
yes exit no
Motor Test Motor testing procedure

yes exit no
Solenoid Test
yes exit no
MotTest:Plate
yes exit no
Plate motor testing procedure
MotTest:Manifold
yes exit no
Manifold motor testing procedure
MotTest:Pumps
yes exit no
Pump testing procedure
To enter the Motor Testing procedure, press yes when the following message is
displayed:
Motor Test
yes exit no
MotTest:Plate
yes exit no
Use no to select which type of motor is to be tested.

Plate Motor Plate support movement (forwards and backwards)
Manifold Motor Manifold movement (up and down)
Pumps Aspirating, dispensing and aerosol pumps switched on or off
Plate Motor Testing

Motor Test
yes exit no
MotTest:Plate Plate motor testing procedure

yes exit no
MotTest:Manifold
yes exit no
Platemotor: X X = Status of home position switch

- + exit home Press '-' to move the plate support
out of the instrument.
Press '+' to move the plate support
into the instrument.
Press 'home' to return the plate support
to the home position.
Press 'exit' to exit from plate motor
testing procedure
MotTest:Plate
yes exit no
To test the plate support motor, press no until the following message is
displayed:
MotTest:Plate
yes exit no
Press yes to enter the plate motor testing procedure and the following message
is displayed:
Platemotor: X
- + exit home
Where X is the displayed status of the home position switch.

st nd
Use the 1 and 2 keys to move the plate support.
Pressing the - and + keys moves the plate support out of the instrument.
When the plate support has been moved away from the home position, pressing
home returns the plate support to the home position.
When the plate support motor testing has been completed, press exit and the
MotTest:Plate
yes exit no
Press no to select the next motor to be tested or press exit to return to the Select
Testing option.
Manifold Motor Testing

Motor Test
yes exit no
MotTest:Manifold Plate motor testing procedure

yes exit no
MotTest:Pumps
yes exit no
Manifoldmotor: X X = Status of home position switch

- + exit home Press '-' to rotate the motor anticlockwise
Press'+' to rotate the motor clockwise
Press 'home' to return the manifold
to the home position.
Press 'exit' to exit from manifold motor
testing procedure
MotTest:Manifold
yes exit no
To test the manifold motor, press no until the following message is displayed:
MotTest:Manifold
yes exit no
Press yes to enter the manifold motor testing procedure.

Manifoldmotor: X
- + exit home
Where X is the displayed status of the home position switch (1 = home position)
st nd
Use the 1 and 2 keys to rotate the manifold motor.
Pressing - rotates the motor anticlockwise.
Pressing + rotates the motor clockwise.
When the manifold has been moved away from the home position, pressing
home returns the manifold to the home position.
When the manifold motor testing has been completed, press exit and the
MotTest:Manifold
yes exit no
Testing option.
Pump Testing
Motor Test
yes exit no
MotTest:Pumps Plate motor testing procedure

yes exit no
MotTest:Plate
yes exit no
0 XXX 0 Press Asp to switch the aspirating pump

Asp Dis exit Aer on (1) and off (0)
Press Dis to switche the dispensing pump
on and off (when pump is rotating)
XXX = number of rotations performed)
Press Aer to switch the aerosol pump
on (1) and off (0)
Press exit to exit from the Pump Testing procedure
MotTest:Pumps
yes exit no
To test the pump motors, press no until the following message is displayed:
MotTest:Pumps
yes exit no
Press yes to enter the Pump Motor Testing procedure.

0 XXX 0
Asp Dis exit Aer
Pressing Asp starts and stops the aspirating pump.

Pressing Dis starts and stops the dispensing pump.
Pressing Aer starts and stops the aerosol pump (if fitted).
Aspirating Pump
To test the Aspirating pump, press Asp to start the pump.
When the pump is started, the pump status is changed from 0 to 1.
1 XXX 0
Asp Dis exit Aer
Check that the aspirating pump is running.

If the pump is not running, the possible causes are:
Pump is defective Replace Pump
No power to the pump Replace Main Board, Power Supply Board
Aerosol Pump*
To test the Aerosol pump, press Aer to start the pump.
When the pump is started, the pump status is changed from 0 to 1.
0 XXX 1
Asp Dis exit
Check that the Aerosol pump is running.

Dispensing Pump
To test the Dispensing pump, press Dis to start the pump.
When the pump is started, the number of rotations performed (XXX) is displayed.
0 XXX 1
Asp Dis exit
In firmware versions with the prime valve, the Aerosol pump doesn't function.
If the pump is not running or there is no current flow to the pump, the following
message is displayed:
0 0 0
Disp.Sens.Err.!!
The instrument is now locked with this error message, switch the instrument off
and on again.
When the pump motor testing has been completed, switch all the pumps off and
press the exit.
MotTest:Pumps
yes exit no
Testing option.
* In firmware versions with the prime valve, the aerosol pump does not function.
Solenoid Testing
This option is used to check that the various solenoid valves are functioning
correctly.
Function Test
yes no exit
Solnoid Test
yes exit no
Sensor Test
yes exit no
Disp. Valve: Off

yes exit no
Switch dispensing solenoid
valve on and off
Valve Ch 1: Off
yes exit no
Switch channel 1 solenoid
valve on and off
Valve Ch 2: Off
yes exit no
valve on and off
Valve Ch 3: Off
yes exit no
valve on and off
Valve Ch 4: Off
yes exit no
valve on and off
Prime Valve: Off
yes exit no
Switch prime solenoid valve on
and off
This valve is no longer fitted to the instruments
Pinch Valve
Solenoid Valve
Inlets for channels 1 - 4
The number of solenoid valves that is fitted is dependent on the number of

dispensing channels fitted.
To select the Solenoid Testing procedure, press no until the following message is
displayed:
Solenoid Test
yes exit no
Press yes to enter the testing procedure and the following message is displayed:
Disp.Valve: OFF
yes exit no
Press yes to switch the dispensing solenoid valve on and off.

Check that the solenoid valve is activated (pinch valve in) when valve is switched
on.
Press no to select the next solenoid valve and the following message is
displayed:
Valve Ch. 1: Off

yes exit no
Press yes to switch the channel 1 solenoid valve on and off.

displayed:
Valve Ch. 2: Off

yes exit no

displayed:
Valve Ch. 3: Off

yes exit no

displayed:
Valve Ch. 4: Off

yes exit no

displayed:
Prime Valve:Off
yes exit no
Press yes to switch the channel prime valve on and off.

Press no to select the next valve and the following message is displayed:
When all the required valves have been tested, press exit and the following
Solenoid Test
yes exit no
Press no to select the next testing procedure or press exit to return to the
Function Test option.
Sensor Function Test

This option is used to check that the various sensors are functioning correctly.
Function Test
yes no exit
Sensor Test
yes exit no
Test Fill Verif.

yes exit no
Sensor Test
XX XXX exit
To select the Sensor Testing procedure, press no until the following message is
displayed:
Sensor Test
yes exit no
Press yes and the instrument disables all the motors and the following message
is displayed:
Sensor Test
MM AXP exit
Where MM AXP WF is the displayed status of the various sensors

MM Manifold type sensors (00 = no manifold, 01 = 8 way,
10 = 16 way, 11 = 12 way)
A Manifold arm home position sensor (1 = home position)
X Plate mismatch sensor (1 = plate mismatch)
P Plate support home position sensor (O = home position)
Move the various parts to check that the sensor are working correctly.
When the sensor testing has been completed, press exit and the following
Sensor Test
yes exit no
When the sensor testing has been completed, press exit and the following
Sensor Test
yes exit no
Press no to select the next Testing procedure or press exit to return to the
Fill Verification Testing

If the Fill Verify manifold is fitted, it can be tested using the following procedure.
To select the Fill Verify testing procedure, press no until the following message is
displayed:
Test Fill Verif.

yes exit no
XX: yyyyyyyyyyyy
Asp Dis exit CLR
Where XX: is the type of manifold fitted (8,12 or 16) and yyyyyyyyyyyyy is the
status of the needles.
st nd
Use the 1 and 2 keys to start and stop the aspirating and dispensing pumps.
Pressing Asp starts and stops the aspirating pump.
Pressing Dis starts and stops the dispensing pump.
When the instrument is dispensing liquid the status of the needles changes.
Press the CLR key to clear the display and then redisplay the new status.
When the testing has been completed, press exit and the following message is
displayed:
Test Fill Verif.

Yes exit no
Barcode Reader
If the Barcode Reader option is fitted, it can be tested using the following
procedure.
To select the Barcode Testing procedure, press no until the following message is
displayed:
Barcode Test
yes exit no
exit
Move the barcode reader over a barcode strip, when the barcode strip has been
read the instrument beeps and displays the read barcode number. For example:
AE 2345
exit
This procedure can now be repeated for another barcode strip.

When the testing has been completed, press exit and the following message is
displayed:
Barcode Test
yes exit no
Liquid Detection
If the Liquid Detection Option is fitted, it can be tested using the following
procedure.
To select the Liquid Detection testing procedure, press no until the following
Liq.Sens.
yes exit no
Liq.Sen.1 XXX Y
- + exit yes
Where XXX is the displayed value and Y is the limit (0 = lower 1 = higher)
Use the - and + keys to select the required liquid sensor to be tested.
(1 - 4 & W*)
When the required sensor is displayed, gently press on the bottle and check that
the displayed value has increased. Carefully lift the bottle and check that the
value has decreased.
When the testing has been performed on the selected liquid sensor, use the - and
+ keys to select the next required sensor.
When the testing has been completed on all the sensors, press exit and the
Liq.Sens.
yes exit no
* 1 - 4 and W are the five sensors
1 Green
2 Gray
3 Blue (4 liquid channels)
4 Purple (4 liquid channels)
W Waste
Show Detected CPU
uC: 80C517A uC: 80537

exit yes exit yes
Shows which version of micro-controller that is used on the main board.
Exiting the Function Test

When all the required function tests have been performed, the function test
procedure is exited by pressing exit.
Service
other yes
Function Test Function Testing procedure

yes exit no
Select Options
yes exit no
Motor Test
yes exit no
Motor testing procedure
Solenoid Test
yes exit no
Sensor Test
yes exit no
Sensor testing procedure
Test Fill Verif.

yes exit no
Fill Verify testing procedure
Barcode Test
yes exit no
Barcode Reader testing procedure
Orbit.Shake:
yes exit no
Orbital Shaking testing procedure
(This option is not used.)
Liq.Sens.
yes exit no
Liquid Detection testing procedure
uC: 80517A uC: 80537

or
exit yes exit yes
Function Test
yes exit no
Press yes to re-enter the Function Test submenu.

Press exit to exit from the Service menu.
Press no to select the next option in the Service menu.
Select Options
This option is used to select which options that are fitted to the instrument.
Select Options
Select fitted instrument options
yes exit no
Adj Dispens Pump
yes exit no
Code:
Enter required code '121418' (for firmware V4.20 and higher)
1 2 1 4 1 8 (Enter ‘1248’ for firmware V4.01 up to but not including V4.20)
Waste Full: XXX Use '-' and '+' key to select if Waste Full
is fitted.
- + other yes Press 'yes' to confirm and save setting
Press 'other' to move to next option
Barcode: XXX Use '-' and '+' key to select if Barcode

- + other yes
is fitted.
Press 'yes' to confirm and save setting
Printer: XXX Use '-' and '+' key to select if Printer is fitted.
- + other yes Press 'other' to move to next option
Fill.Verif.: XXX Use '-' and '+' key to select if Fill

- + other yes
is fitted.
Manif. Det.: XXX Use '-' and '+' key to select if Manifold
- + other yes is fitted.
Aerosol Pump: XXX Use '-' and '+' key to select if Aerosol
Liq.Sens. XXX Use '-' and '+' key to select if Liquid

Orbit.Shake: XXX Use '-' and '+' key to select if Orbital

- + other yes is fitted. This option is not used.
Drip Mode: XXX Use '-' and '+' key to select if Drip Mode is used.
- + other yes Press 'yes' to confirm settings
Press 'other' to move to next option without saving setting
Options END Press 'yes' to exit procedure

other yes Press 'other' to repeat procedure
Adj. Prime
yes exit no
Select Options
yes exit no
Press yes to enter the Select Options submenu and the following message is
displayed:
Code:
1 2 4 8
The Select Options submenu can only be entered by entering the required
code: 1248
When the code has been correctly entered, the following message is displayed:
Waste Full: NO
- + other yes
Use the - and + keys to set if the Waste Full Sensor option is fitted and to be
used.
NO = option not fitted and not used
YES = option fitted and used
Pressing other moves to the next option without saving the setting.
When the required setting is displayed, press yes to save the setting and the
Barcode: NO
- + other yes
Use the - and + keys to set if the Barcode option is fitted and to be used.
Printer: NO
- + other yes
Use the - and + keys to set if the Printer option is fitted and to be used.
Fill Verif.: NO
- + other yes
Use the - and + keys to set if the Fill Verify option is fitted and to be used.
Manif Det.: NO
- + other yes
Use the - and + keys to set if the Manifold Detection option is fitted and used.
Aerosol Pump NO
- + other yes
Use the - and + keys to set if the Aerosol Pump option is fitted and to be used.
Liq.Sens. NO
- + other yes
Use the - and + keys to set if the Liquid Detection option is fitted and to be used.
Orbit.Shake: NO
- + other yes
This option is not used.

Drip Mode: NO
- + other yes
This option is only available together with the cell wash manifold (M8, M12, M16).
Use the - and + keys to set if the Drip Mode option is fitted and to be used.
When selecting yes the dispensing pump must be calibrated for Drip Mode
speed. For more information, see next page - Adjust Dispensing Pump.
Options END
other yes
Press other to return to the start of the select options submenu.

Press yes to exit from the Select Options submenu and the following message is
displayed:
Adj. Prime Pos.
yes exit no
Adjust Dispensing Pump

This option is used to calibrate the dispensing pump so that the correct volume of
solution is dispensed with each manifold that is used.
Service
other yes
Adj Dispens Pump

yes exit no Adjust Dispensing Pump option
Service
other yes
Disp. Speed X Select required dispensing speed

- + exit yes
(3 or drip)
Weight_300: xx.x
- + exit yes Enter weight for 300 µl
Weight_100: xx.x
- + exit yes Enter weight for 100 µl
Adj Dispens Pump Repeat procedure for another

yes exit no
dispensing speed
The delivered instrument has been calibrated for the supplied manifolds.
Calibration must be performed if another manifold is to be used or if the fitted
manifold is exchanged for another manifold of the same type.
Before the calibration procedure can be performed, programs that dispense
300 µl and 100 µl solution into each well must be defined with the required
dispensing speed.
The dispensing pump is calibrated using the following procedure:
Weight 300
1. Accurately weigh a clean dry microplate (x)
2. Select the program that dispenses 300 µl of solution into each well.
3. Accurately weigh the microplate containing the solution. (y)
4. Calculate the weight of solution (y - x = Weight 300)
Weight 100
1. Accurately weigh a clean dry microplate (x)
2. Select the program that dispenses 100 µl of solution in to each well.
3. Accurately weigh the microplate containing the solution. (z)
4. Calculate the weight of solution (z - x = Weight 100)
Enter the Service menu and scroll through the options until the following message
is displayed:
Adj Dispens Pump

yes exit no
Disp. Speed X
- + exit yes
Use the - and + keys to select the required dispensing speed.(3 or drip)
When the required dispensing speed is displayed, press yes and the following
Weight_300: xx.x
- + exit yes
Where XX.X is the displayed weight of 300 µl solution in each well.

st nd
If the displayed weight is not correct use the 1 and 2 keys to scroll the correct
weight.
Pressing - decreases the weight by 0.1 g.
Pressing + increases the weight by 0.1 g.
When the required weight is displayed, press yes and the following message is
displayed:
Weight_100: xx.x
- + exit yes
Where XX.X is the displayed weight of 100 µl solution in each well.

nd
If the displayed weight is not correct, use the 1st and 2 keys to scroll the correct
weight.
Pressing - decreases the weight by 0.1 g.
Pressing + increases the weight by 0.1 g.
When the required weight is displayed, press yes and the following message is
displayed:
Adj Dispens Pump

yes exit no
When the calibration has been performed press exit to exit from the Service
menu or press no to select the next option in the Service menu.
Repeat the procedure with Disp. Rate: Drip if this option is enabled.
Ensure that the calibration procedure is repeated if the weight (300 µl) is
greater than 33 g or less than 24 g before calibrating the instrument for the
first time.
Adj. Prime Pos.

This option is used to adjust the position of the plate support for the Prime/Rinse
procedures.
The defined position for 8 and 12 way manifolds is different to that of the 16 way
manifold. It may be necessary to adjust the position of the 16 way manifold
otherwise the solution could be dispensed outside the waste sink.
Use the following procedure to adjust the Prime Position.
Service
other yes
Adj Prime Pos.

yes exit no Adjust Prime Position option
Service End
yes no
Prime Pos.: XXX Use the '-' and '+' keys to position
- + exit yes
the waste sink correctly under
the manifold
Service End
yes no
Adj.Prime Pos.
yes exit no
Press yes to enter the Prime Position following message is displayed:
Prime Pos.: 75
- + exit yes
Use the - and + keys to move the position of the plate support.
Pressing - moves the plate support more under the manifold.
Pressing + moves the plate support out from under the manifold.
Waste Sink
Plate Support
When a key is pressed, the instrument first moves the plate support to the home
position and then moves the plate support to the new prime / rinse position.
Each press of the key moves the plate support by five steps.
When the plate support is correctly positioned so that no solution is dispensed
outside the waste sink, press yes, the position is saved and the following
Service End
yes no
Adj. Plate Ref.

The Adjust Plate Reference is used to define the plate position in order that the
manifold needles are correctly positioned. The adjustment should always be
positioned as shown below for all manifolds. The procedure is the same for
manifolds; 8, 12 and 16. Place the adjustment plate on the plate support and
enter the Service menu.
alignment
points for the
8 manifold
needle
Adjustment Plate
Use the - and + keys to move the manifold needles in the X (left and right) and Z
(up and down) directions. The needles must be set exactly on the crosses
marked on the adjustment plate.
Service
other yes
Adj Plate Ref.

yes exit no Adjust Plate Reference option
Service
other yes
Ref. Pos. X: 390 Position the manifold needles

- + exit yes
using the '-' and '+' keys to move
left and right
Ref.Pos. Z: 130 Position the manifold needles
- + exit yes
using the '-' and '+' keys to move
up and down
Ref. Pos. OK? Confirm that the set

yes no positions are correct
Init Manifold Manifold is initialized
Service End
This option is used to select if the Service menu is to be exited.
Service End Exit Service Menu
yes no
Clear All ?
yes exit no
Run X:yyyyyyyy Standby Mode

- + other yes
Service End
yes no
Select if the Service menu is to be exited.

Remain in Service Menu
Press no to remain in the Service menu and the following message is displayed:
Clear All ?
yes exit no
Select the required option in the Service menu.

Exit Service Menu
Run X:yyyyyyy
- + other yes
The instrument is now in the Standby mode and can be used to wash a
microplate.
3.3 External Software

3.3.1 Columbus Computer Software
Consisting of WinWash and Download including the operating manual and
RS232 interface cable.
3.3.2 Firmware Update

Consisting of the Download software, the latest version of the firmware 3.x, as
well as 5 language software files (English, German, French, Spanish and Italian)
that can change the on-board language of the Columbus (from firmware level
2.23 onwards) in the field.
3.4 Serial Interface

A description of the serial interface fitted to the instrument.
3.4.1 Hardware Specifications

The instrument is controlled by the computer and the communication between the
computer and the instrument is done through a RS-232-C interface.
The interface specification may be either to the CCITT interface standard or to
the EIA RS-232-C interface standard.
Pin Designation
The illustration below shows the pin assignment of the DB 9 connector fitted to
the instrument.
Pin 5 Pin 1
Pin 9 Pin 6
All connected devices must be approved and listed as per EN 60950, UL 1950 or
CSA C22.2 No. 950 for Data Processing Devices
RS-232-C Interface Lines

The Serial Interface of the instrument is connected to a start-stop synchronized
serial RS-232-C circuit.
The list below names the interface lines of the instrument's 9 pin connector.
PIN Number DESCRIPTION
1 RS 485 IN/OUT (Option)
2 (RS232) TD Transmit Data
3 (RS232) RD Receive Data
4 RS 485 IN/OUT
5 GND Ground
6 RS-config: if connected to GND RS 485, if open RS 232
is active (only if jumper x100 on the mainboard is open!)
7 No connection
8 RS232 CTS Clear To Send
9 +24V INPUT (Fused 4AF)
The connecting cable used to connect the instrument to the computer should be
wired as given below:
INSTRUMENT COMPUTER
TD connected to RD
RD connected to TD
GND connected to GND
RTS connected to CTS
DSR connected to DCD and DTR
Use the computer handbook to find the correct pin connections.
Instrument Computer 25 pin Instrument Computer 9 pin

1 1 1 1
2 2 2 2
3 3 3 3
4 4 4 4
5 5 5 5
6 6 6 6
7 7 7 7
8 8 8 8
9 20 9 9
Synchronization and Data Format

For the instrument to communicate with the computer correctly, the instrument
and the computer must be set for the same communication parameters.
Check that following parameters are set correctly on both the computer and the
instrument:
1. Start bit length: 1 Bit
2. Stop bit length: 1 Bit
3. Data format: 8 Bits
4. Parity bit: None
5. Baud Rate: 9600 Baud
6. Mode: half duplex
Daisy Chaining Instruments

The instrument can be connected to a computer, which controls a number of
instruments using a Daisy Chain cable. (RS 485 version only).
Da isy Cha in Ca b le
Co m p uter
Ad d re ss #30
Wa sher 1 Wa sher 2 Wa sher 3

Ad d re ss #31 Ad d re ss #32 Ad d re ss #33
Each of the instruments must be defined with a different device address

Each instrument can be controlled by sending the commands to the required
device address.
To select a different device address, start the Remote Mode with the default
device address (#31) and send the command to X 37 (to select the device
address #37).
3.4.2 Contents
A. Protocol 3 – 65
B. Commands - Overview 3 – 67
C. Commands - Description 3 – 69
1. Switch communication on/off 3 – 69
2. Starting and Stopping a Program / Prime 3 – 69
Procedure /Rinse Procedure
3. Define Programs 3 – 70
Define Header / Procedure 3 – 70
Define Program Lock Status 3 – 70
Common parameters 3 – 71
Procedures 3 – 71
4. Upload Program 3 – 73
Upload Header 3 – 73
Upload Procedure 3 – 73
Read Program Lock Status 3 – 73
5. Clear Program 3 – 74
6. Define Plates 3 – 74
Write Plate name / Define Plate Lock Status 3 – 74
7. Upload Plate Data 3 – 75
Read Plate name / Plate Lock Status 3 – 75
8. Define Rinse Parameters 3 – 75
9. Upload Rinse Parameters 3 – 76
Read Prime Time
10. Read Device Information 3 – 76
11. Read Header & Firmware Version 3 – 76
12. Define Power on Header 3 – 76
13. Read Option Status 1 & 2 3 – 77
14. Define Option Status 1 & 2 3 – 78
15. Read Device Status 3 – 78
16. Read Number of Liquid Channels 3 – 79
17. Read Manifold Information 3 – 79
18. Define Manifold Type 3 – 79
19. Read Dispense Rates 3 – 79
20. Define Dispense Rates 3 – 80

21. Read Aspirate Rates 3 – 80
22. Define Aspirate Rates 3 – 80
23. Calibrate Dispense Pump 3 – 81
24. Read Total Test Time & Power On Time 3 – 81
25. Clear Total Test Time & Power On Time 3 – 81
26. Adjust Times 3 – 82
27. Change Device Address 3 – 82
28. Read Plate Identity (Barcode) 3 – 82
29. Clear All 3 – 82
30. Start Flash Download Mode 3 – 82
31. Read / Write Settings 3 – 83
32. Write time & date 3 – 83
33. Enable / Disable Fill Verification 3 – 83
34. Read Fill Verification Needle Status 3 – 84
D. Examples for remote control - command sequences 3 - 85

Enter Remote Mode 3 – 85
Leave Remote Mode 3 – 85
Change Device address 3 – 85
Start Program 3 – 86
Start Prime 3 – 86
Download Program 3 – 87
Download Plate 3 – 90
Download Rinse Parameter 3 – 91
3.4.3 A. Protocol
1. Data format
Baud Rate: 9600 Baud
Databits: 8
Parity: No
Stop Bit: 1
Mode: Half duplex
Command Block Format (Master -> COLUMBUS)

hex dec keyboard
0 Synchronization 0xFF 255 ALT-'255'
character
1 STX 0x02 2 ALT-'2' / ^B
2 Device Address 0x32 50 '2' (example)
(Columbus Add.)
3 Sequence Number 0x31 49 '1'
4 Datablock (n
characters long)
4+n ETX 0x03 3 ALT-'3' / ^C
5+n Checksum
Answer Block Format (COLUMBUS -Master)

0 Synchronization character (FFh) 0xFF 255 ALT-'255'
1 STX (^B or 02h) 0x02 2 ALT-'2' / ^B
2 Master Address (30h) 0x30 48 '0'
3 Status Code
4 Datablock (n characters long)
4+n ETX (^C or 03h) 0x03 3 ALT-'3' / ^C
5+n Checksum
6+n Line turn around character 0xFF 255 ALT-'255'
a.) Synchronization character for Bus Synchronization

b.) STX (^B or 02h) start of Command string
c.) Device Address is a hexadecimal number for every device on the bus
(for addressing in 'Daisy Chain')
d.) Master Address is the address of the host system. This address is always 30hex.
e.) Status includes device status (Ready, Busy) and the error codes.
f.) Sequence Number (for repetitions of commands at incorrect transmission).
g.) Datablock includes data and/or commands received from or sent to the washer.
h.) ETX (^C or 03h) defines the end of the command/data-string (except CS character).
i.) Checksum is the last byte of the command/data-string. All bytes (except
synchronization and checksum) are XOR-calculated. The receiver compares the sent
checksum with the calculation. When they are different the command/data-string is
ignored (Error).
j.) Line turn around character - to make sure that the last byte (Checksum) is received
complete. It is possible that this character will not be sent complete.
3.4.4 Sequence Number

7 6 5 4 3 2 1 0
0 0 1 1 REP SQ2 SQ1 SQ0
SQ0 ... SQ2 1 ... 7 Sequence Number, 0 not allowed

REP 1 Repeated Command
0 First Command
Status Code
7 6 5 4 3 2 1 0
0 1 RDY 0 EC3 EC2 EC1 EC0
EC0 ... EC4 0 No Error

1 Init Error
2 Invalid Command
3 Invalid Operand
4 Invalid Command Sequence
5 System Error (not used)
6 res.
7 Plate Mismatch
8 Plate Transport Error
9 Manifold Transport Error
10 Dispense Pump Error
11 Fill Verification Error
12 Manifold Error
13 Waste Full Error
14 Test Not Defined
15 Command Overflow
16 Fill Verification Function Error
17 Fill Verification Aspiration Error
RDY 1 Ready
0 Busy
3.4.5 B. Commands - Overview

Command Abr. Parameter 1 Parameter 2 Page
Read Dispense Rates A1 08/12/16 3 – 79
Define Dispense Rates A2 08/12/16 Drip 005 - 050 3 – 80
6x 000 - 999
Read Aspirate Rates A3 3 – 80
Define Aspirate Speed A4 5x 000 - 999 3 – 80
Read Total_ Test_Time & A6 3 – 81
Power_On_Time
Clear Total_ Test_Time & A7 3 – 81
Power_On_Time
Set_Time (Sort_of_Time, Time) AT p/r/a 00 - 99 (24) 3 – 82
Download_Program (Prog. Nr., ...) D 01 - 30 3 – 70
Clear Program (Prog. Nr) DC 01 - 30 3 – 74
Download Header/Procedure DL 00 - 58 3 – 70
(Select, ...)
Remote_Mode_OFF E 3 – 69
Start_Flash_Load_Mode FL 3 – 82
Read Fill Verification Needle Status FR 3 – 84
Enable / Disable Fill Verification FV 0/1 3 – 83
Start_Prime (Liquid Channel) GP 1-4 3 – 69
Start_Rinse (Modus, Liquid Channel) GR 0-3 0-4 3 – 69
Stop Program / Prime procedure HP 3 – 69
Stop_Rinse HR 3 – 69
Read_Device_Status I 3 – 76
Define Options 2 LA '0' , '1' 3 – 78
Define Power On Header LH 'Header' 3 – 76
Select Options LO '0' , '1' 3 – 78
Read_Manifold_Information M 3 – 79
Define Manifold Type MA 08 / 12 / 16 3 – 79
Define_Program_Lockstatus N ´#´,´ ´ 3 - 70
Read_Program_Lockstatus NL 3 – 73
Read Options_1 O 3 – 77
Read Options_2 OA 3 – 77
Read Plate ID (Barcode) PI 3 – 82
Download_Plate (Plate Nr., ...) PD 1-9 ... 3 – 74
Read_Platename (Plate Nr.) PR 1-9 3 – 75
Upload_Plate (Plate Nr.) PU 1-9 3 – 75
Write_Platename (Plate Nr., ...) PW 1-9 ´Plate name´ 3 – 74
Query Q 3 – 78
Download_Rinse_Para. (Rtime,ARtime) RD 01 - 99 01 - 24 3 – 75
Upload_Prime_Parameter RP 3 – 76
Upload_Rinse_Parameter RU 3 – 76
Read Setting RS 0-8 0-4 3 – 83
Start_Program (Prog. Nr., Nr. of Strips) S 01 - 30 01 - 12 (8, 6) 3 – 69
Start_Program (Prog. Nr., Sel. Strips) ST 01 - 30 12 / 8 x 0 / 1 3 – 69
Upload_Program(Prog.Nr.) U 01 - 30 3 – 73
Upload Header/Procedure (Select, ...) UL 00 - 58 3 – 73
Calibrate Dispense Pump (Speed 1 – 5) W 00.0 – 3 – 81
99.9
Calibrate Dispense Pump (Speed Drip) WD 00.0 – 3 – 81
99.9
Write Settings WS 0-8 3 – 83
Write Time & date WT ... 3 – 83
Read_Number_Of_Solenoids V 3 – 79
Change_Device_Address (New Address) X 0x31 - 3 – 82
0x3F
Clear_All XX 3 – 82
Remote_Mode_ON Y/! 0 / 1 / 'M' 3 – 69
Read_Header & Firmware Version Z/? 3 – 76
For Genalyser – Firmware Version 3.06

Command Abr. Parameter Validity Page
Abort Program FA None Run time 3 – 84
Continue Program FC None Run time 3 – 85
Last Processed Strip FS None Run time 3 – 85
Fill Verification Status F? None Always 3 – 85
3.4.6 Commands - Description

1.Switch Communication On/Off
Command Parameter Answer
Remote ON ! or Y <p> p = 0: send no end after +CW
program & prime
p='M': MINILYSER Mode
(no 'BUSY' in 'ACK'
else: send end.
Remote OFF E - ACK
2. Starting and Stopping a Program / Prime Procedure /Rinse Procedure

Start Program 1 S<p1><p2> p1: number of program ACK
p2: number of strips
Start Program 2 ST<p1><p2> p1: number of program ACK
p2: 8(12) x 0/1 selection of
strips
Start Prime GP<p> p: liquid channel ACK
Start Rinse GR<p1><p2> p1: Rinse mode ACK
0:SOAK 1: DAY
2: NIGHT 3: AUTO
p2: liquid channel
(0 = all channels)
Stop Rinse HR - ACK
Stop Program/Prime HP - ACK
3. Define Programs
Download_Program D<p1><p2> p1: Program number ACK
<p3><p4>... p2: Number of following
columns
p3: common parameters
p4: parameters of
procedures
Define Header
Download_header DL<p1><p2> p1: Selection 00: Header ACK
<p3><p4>... p2: Program number
p3: number of following
columns
Define Procedure
Download_procedure DL<p1><p2> p1: Procedure number 01 - 59 ACK
<p3><p4>... p2: Program number
p3: parameter of procedures
Define Program Lock Status

Define Program Lock Status N<p1> p1: Program number ACK
<p2>
p2: Lock Status
P2 = ´#´ ⇒ Prog. locked
P2 = ´ ´ ⇒ Prog. unlocked
Common Parameters
Program name: '' 1st & 8th character (8 characters)
Plate type: 1-9 9th character (1 character)
th th
Manifold type: 08 / 12 / 16 10 & 11 character (2 characters)
Dispense rate: 0 - 5 /0=drip 12th character (1 character)
th
Aspirate rate: 1-5 13 character (1 character)
Aspirate mode: 0-3 14th character (1 character)
Global / Final Asp.

0: normal / normal
1: crosswise /
crosswise
2: normal /
crosswise
3: crosswise /
normal
Working mode: 0/1 plate / strip mode 15th character (1 character)
Strip selection: 0/1 NO / YES 16th character (1 character)
Printer mode: 0/1/2 NO / YES / ASK 17th character (1 character)
Final Asp. Time: 00 - 20 (in sec)/ 00 = NO 18th & 19th (2 characters)
character
Final Asp. Speed: 00 - 20 (in mm/s) 20th & 21th (2 characters)
character
Procedures
1. Wash
Code: 'W' 1 character
1. Parameter: Aspirate Speed 01-20 2nd & 3rd character
2. Parameter: Dispense / Aspirate Position 0/1 4th character
Parameter Dispense Postion Aspirate position
0 Bottom Bottom aspirate
1 Overflow Bottom aspirate
2 Bottom Bottom wash
3 Overflow Bottom wash
3. Parameter: Dispense Channel 1-4 5th character
4. Parameter: Dispense Volume 0050-3000 6th – 9th character (in µl)
2. Aspirate
Code: 'A' 1th character
1. Parameter: Aspirate Time 01-20 2th & 3th character
2. Parameter: Aspirate Speed 01-20 4th & 5th character
3. Dispense
Code: 'D' 1th character
1. Parameter: Dispense Position 0/1 2th character
2. Parameter: Dispense Channel 1-4 3th character
3. Parameter: Dispense Volume 0050-0400 4th & 7th character (in µl)
4. Soak
Code: 'S' 1 character
1. Parameter: Soak Time 001-999 2nd - 4th character in sec
2. Parameter: Shake Speed 0-4 5th character
0 No shaking
1-3 Linear shaking
4 Orbital shaking
3. Parameter: Shake Mode 0-2 6th character
Parameter: Shake Speed (orbital) 0-2 6th character
5. End
a. End of Cycle
Code: 'E' 1st character
1. Parameter: Type of END 0 2nd character
2. Parameter: Cycle Number 00-30 3rd & 4th character
3. Parameter: Repeat Number 00-09 5th & 6th character
4. Parameter: Proc. in Cyc. 01-59 7rd & 8th character
b. End of Program
Code: 'E' 1st character
1. Parameter: Type of END 1 2nd character
4. Upload Program
Upload_Program U<p1> p1: Program p2: number of following
number columns
p4: parameters of
procedures
(see 3. Define Programs)
Upload Header
Upload_header UL<p1><p2> p1: Selection p3: number of
<p3><p4> 00: Header following columns
p2: Program p4: common
number parameters
Upload Procedure
Upload_procedure UL<p1><p2> p1: Procedure p3: parameters
<p3> of procedures
number
p2: Program
number
Read Program Lock Status

Read Program Lock Status NL p1: Program p2: Lock Status
number
p2 = ´#´ ⇒ Prog.
Locked
P2 = ´ ´ ⇒ Prog.
unlocked
5. Clear Program
Clear_Program DC<p1> p1: Program ACK
number
6. Define Plates
Download_Plate PD<p1><p2> p1: Plate Number ACK
<p3><p4>... p2: Bottom Form
p3: Bottom Aspirate
Position
p4: Bottom Wash
Position
p5: Overflow Position
p6: Dispense Position
p7: Aspirate Position 1
p8: Aspirate Position 2
Plate-Nr .: 1-9
Bottom form 0/1 (0 -> flat, 1 -> round)
Bottom aspirate position (4 characters)
Bottom wash position ( - ║- )
Overflow position ( - ║- )
Dispense position ( - ║- )
Aspirate position 1 ( - ║- )
Aspirate position 2 ( - ║- )
Write Plate name / Define Plate Lock Status

Write_Platename PW<p1>< p1: Plate Number ACK
p2>
p2: ´PLATENAME´
Plate-Nr .: 1-9
Platename (9 characters): 1 - 8: Name
9: Lock status of ´#´ ⇒ Plate locked
plate
´ ´ ⇒ Plate unlocked
7. Upload Plate Data

Upload_Plate PU<p1> p1: Plate Number <p2><p3><p4>...
• p2: Bottom Form
• p3: Bottom Aspirate Position
• p4: Bottom Wash Position
• p5: Overflow Position
• p6: Dispense Position
• p7: Aspirate Position 1
• p8: Aspirate Position 2
Read Plate name / Plate Lock Status

Read_Platename PR<p1><p2> p1: Plate Number p2: ´PLATENAME´
Plate-Nr .: 1-9
Platename (9 characters): 1 - 8: Name
9: Lock status of plate ´#´ ⇒ Plate locked
´ ´ ⇒ Plate unlocked
8. Define Rinse Parameters

Download_Rinse_ RD<p1><p2> p1: Rinse Time ACK
Parameter
p2: Auto Rinse
Time
p1: Rinse Time (01 - 99 / in seconds)

p2: Auto Rinse Time (01 - 24 / in hours)
9. Upload Rinse Parameters

Upload_Rinse_Parameter RU - <p1><p2>
p1: Rinse Time (01 - 99 / in seconds)

p2: Auto Rinse Time (01 - 24 / in hours)

Read_Prime_Time RP - <p1>
p1: Prime Time (01 - 99 / in seconds)
10. Read Device Information

Read_Device_Information I - <p1>(<p2><p3>)
p1: Sort of Procedure (P, R, T, S)

P: Prime active
R: Rinse active
T: Testrun (Program) active
S: Standby
p1 = 'T':
p2: Number of Program
p3: Number of Strip in Progress
11. Read Header & Firmware Version

Read_Model&FW-Version Z? - <p1>
p1: String of 32 characters

• 0 ... 15: Power On Header
• 16 ... 31: Firmware Version
12. Define Power On Header

Define_Header LH <p1> ACK
p1: String of 16 characters (Power On Header)
13. Read Option Status 1 & 2

Read_Options 1 O - <p1>
p1: String of 8 characters ('0' or '1' / 0 = no, 1 = yes)

1. Position: Orbital Shake
2. Position: Manifold Error Detection
3. Position: Aerosol Protection
4. Position: Manifold Detection
5. Position: Fill Verification
6. Position: Serial Printer (Real Time Clock)
7. Position: Barcode Pen
8. Position: Waste Full Detector

Read_Options 2 OA - <p1>

1. Position: reserved
7. Position: Dispense Drip Mode
8. Position: Liquid Sensors
14. Define Option Status 1 & 2

Define_Options 1 LO <p1> ACK

1. Position: Orbital Shake
2. Position: Manifold Error Detection
3. Position: Aerosol Protection
4. Position: Manifold Detection
5. Position: Fill Verification
6. Position: Serial Printer (Real Time Clock)
7. Position: Barcode Pen
8. Position: Waste Full Detector
Define_Options 2 LA <p1> ACK

7. Position: Dispense Drip Mode
8. Position: Liquid Sensors
15. Read Device Status

Query Q - ACK
Used to check the Status Code of the communication (Busy/Ready & Error
Codes)
16. Read Number of Liquid Channels

Read_Channels V - <p1>
p1: 1 ... 4 (Number of Liquid Channels)
17. Read Manifold Information

Read_Manifold M - <p1>
p1: 00 / 08 / 12 / 16 (Number of Dispense Needles / Manifold, 00 = no

Manifold)
18. Define Manifold Type

Define_Manifold MA <p1> ACK
p1: 08 / 12 / 16 (Number of Dispense Needles)
19. Read Dispense Rates

Read_Disp_Rates A1<p1> p1: Manifold Type <p2,...<p8>
p1: Manifold Type (00 / 08 / 12 / 16)

p2: Dispense Rate for Speed 'drip' (3 characters) (005 – 050)
Impulses on dispensing pump optical switch per 100ms
p3: Dispense Rate for Speed Nr. 1 (3 characters) (000 - 999: 00.0% to 99.9%)
p4: Dispense Rate for Speed Nr. 2 (3 characters)
p8: Dispense Rate for priming (3 characters)
20. Define Dispense Rates

Define_Disp_Rates A2<p1>...<p8 p1: Manifold Type <p2,...<p8>
>
p2: Disp. Rate for 'drip'
p3: Disp. Rate Speed 1
p8: Disp. Rate for Prime
p1: Manifold Type (00 / 08 / 12 / 16)

p2: Dispense Rate for Speed 'drip' (3 characters) (005 – 050)
Impulses on dispensing pump optical switch per 100ms
p3: Dispense Rate for Speed Nr. 1 (3 characters) (000 - 999: 00.0% to 99.9%)
p8: Dispense Rate for priming (3 characters)
21. Read Aspirate Rates

Read_Asp_Rates A3 - <p1,...<p5>
p1: Aspirate Rate for Speed Nr. 1 (000 - 999: 00.0% to 99.9%)
p2: Aspirate Rate for Speed Nr. 2 (3 characters)
22. Define Aspirate Rates

Define_Asp_Rates A4 - <p1,...<p5>
p1: Aspirate Rate for Speed Nr. 1 (000 - 999: 00.0% to 99.9%)
23. Calibrate Dispense Pump

Cal_Disp_Pump W<p1>_<p2> p1: Weight at 100 µl ACK
Speed 1 -5
p2: Weight at 300 µl
Cal_Disp_Pump WD<p1>_<p2> p1: Weight at 100 µl ACK
Speed Drip
p2: Weight at 300 µl
('_' between <p1> & <p2> = BLANK)

p1: Measured netto-weight when Dispense 100 µl in MTP. (00.0 to 99.9)
p2: Measured netto-weight when Dispense 300 µl in MTP. (00.0 to 99.9)
24. Read Total Test Time & Power On Time

Read_Test&_PowerOn_Time A6 - p1:Times
p1: Test & PowerOn Time (hhhhhh:mm:ss- hhhhhh:mm:ss)
25. Clear Total Test Time & Power On Time

Clear_Test&_PowerOn_Time A7 - ACK
26. Adjust Times

Set_Time AT<p1><p2> p1: Sort of Time ACK
p2: Time
p1: Sort of Time (p / r / a: p = PRIME, r = RINSE, a = AUTORINSE)

p2: Time @ p / r: 00 - 99 in seconds
@ a: 00 - 24 in hours
27. Change Device Address

Change_Address X p1: new address ACK
p1: selected address (hex) in ASCII - format (2 characters). ('30' to '6F')
28. Read Plate Identity (Barcode)

Read_Plate_ID PI - p1: Plate Identity
p1: 16 characters plate id. (when barcode option is enabled otherwise return
ACK)
29. Clear All

Clear_All XX - ACK
Resets all the device ram to 0xFF and waits for a reset from the external
watchdog.
The device starts again and is out of remote mode.
(All settings are reset to factory settings and all programs are empty).
30. Start Flash Load Mode

Start Flash Load Mode FL - ACK
31. Read / Write Settings

Read Settings RS <p1> <p2>
Write Settings WS <p1><p2> ACK
p1: Number of setting p2: select

0 ... access program 0: not allowed, 1: allowed
1 ... access adjust
2 ... channels 1-4
3 ... skip: Channel x primed? 0: show, 1: skip
4 ... skip: Plate inserted ?
5 ... skip: Waste bottle OK?
6 ... skip: Rinse_solution?
7 ... skip: Prime_solution?
8 ... skip: You should rinse
32. Write Time & Date

Write time & date WT <p1>...<p6> ACK
p1: hour 00 - 24
p2: minute 00 - 59
p3: second 00 - 59
p4: day 01 - 31
p5: month 01 - 12
p6: year 00 – 99
33. Enable/Disable Fillverification

Enable / Disable FV p1: Selection ACK
Fillverification
p1 = 0: disable fillverification p1 = 1: enable fillverification
34. Read Fillverification Needle Status

Command Parameter Answer check
Read Fillverification FR - <p1>
Needle Status
p1: Antwort: ABCD (4 Byte for needle satus )
Byte A,B,C,D – Bit Structure:
Bit Nr. 7 6 5 4 3 2 1 0
Byte A 0 0 1 1 A3 A2 A1 A0
Byte B 0 0 1 1 B3 B2 B1 B0
Byte C 0 0 1 1 C3 C2 C1 C0
Byte D 0 0 1 1 D3 D2 D1 D0
Connection Bits – Needles:

8 Needle Manifold:
Needle 1 2 3 4 5 6 7 8
Buffer A3 A2 A1 A0 B3 B2 B1 B0
12 Needle Manifold:
Needle 1 2 3 4 5 6 7 8 9 10 11 12
Buffer A3 A2 A1 A0 B3 B2 B1 B0 C3 C2 C1 C0
16 Needle Manifold:
Needle rear 1 2 3 4 5 6 7 8
Buffer A3 A2 A1 A0 B3 B2 B1 B0
Buffer C3 C2 C1 C0 D3 D2 D1 D0
Needle front 1 2 3 4 5 6 7 8
When a Bit =1 then the needle had no contact and if the Bit = 0 the needle had
contact.
Unused Bits for a used manifold are always 1.
Needle 1: right side Needle 8 (12): left side of manifold
35. Abort Program

Command Parameter Validity Answer
Abort Program FA - Run time ACK
The current active wash program is stopped.
36. Continue Program

Continue Program FC - Run time ACK
The current active wash program is continued after the Fill Verification Error.
Note: The Fill Verification Error is diagnosed at the end of the faulty strip.
Therefore, the wash step for the faulty strip is fully completed. The FC command
then continues with the next strip or wash step. (The wash step for the faulty strip
may not be completed).
37. Last Processed Strip

Last Processed Strip FS - Run time P2: Strip number
p2: 0 - 12 for 8 / 16 way manifold

0 - 8 with 12 way manifold
Gives a reply of the strip number in which the Fill Verification Error has occurred.
38. Fill Verification Status

Fill Verification Status F? - Always P2: Status Fill
Verification
p2: 1 = On
0 = Off
3.4.7 D. Examples for Remote Control - Command Sequences

Enter Remote Mode
(FF)(STX)(ADR)(SNr.) : Start
Y0 : Command 'Y0'
(ETX)(CS) : End
Meaning HEX DEC Character Keyboard
FF 255 ALT-'255'
STX 02 002 ALT-'2' / ^B
ADR 31 049 1
Snr 31 049 1
DATA: 59 089 Y
30 048 0
ETX 03 003 ALT - '3' / ^C
CS 68 104 ALT - '104'
Leave Remote Mode

E : Command 'E'
(ETX)(CS) : End
FF 255 ALT-'255'
STX 02 002 ALT-'2' / ^B
ADR 31 049 1
Snr 31 049 1
DATA: 45 069 E
ETX 03 003 ALT-'3' / ^C
CS 44 068 ALT-'68'
Change Device Address

(FF)(STX)(ADR)(SNr.) :Start
X37 : Command 'X', New Device Address: 37hex
(ETX)(CS) : End
FF 255 ALT-'255'
STX 02 002 ALT-’2’ / ^B
ADR 31 049 1
Snr 31 049 1
DATA: 58 088 X
33 051 3
37 055 7
ETX 03 003 ALT-’3’ / ^C
CS 5D 093 ALT-’93’
Start Program
S0112 : Command 'S' (Start program Nr. 01 run 12 Strips)
(ETX)(CS) : End
FF 255 ALT-’255’
STX 02 002 ALT-’2’ / ^B
ADR 31 049 1
Snr 31 049 1
DATA 53 033 S
30 048 0
31 049 1
31 049 1
32 050 2
ETX 03 003 ALT-’3’ / ^C
CS 50 080 ALT-’80’
Start Prime
GP1 : Command 'GP' (Start Prime with liquid channel Nr. 1)
(ETX)(CS) : End
FF 255 ALT-’255’
STX 02 002 ALT-’2’ / ^B
ADR 31 049 1
Snr 31 049 1
DATA: 47 071 G
50 080 P
31 049 1
ETX 03 003 ALT - ‘3’ / ^C
CS 27 039 ALT-’39’
Download Program
Example 1
(FF)(STX)(ADR)(SNr.) Start
D0105 Command 'D', Program-Nr. 01,
following columns 05
ASPIRATE Name: 'ASPIRATE’
1082300000000 Plate type 1, Manifold 08, Dispense rate 2, Aspirate rate 3,
Aspirate mode normal/normal (0), Working mode Plate (0),
Strip Selection no (0), Printer Mode no (0),
fin.asp time NO (00), fin asp. speed NO (00)
A0210: Aspirate procedure, Aspirate time 02sec, Manifold speed
10mm/s
E0010202: End of Cycle (0), Cycle Nr.01, Repeat Factor 02, Proc. in
Cycle 02
E1 End of Program (1)
(ETX)(CS) End
Resulting Character Sequence

FF 255 ALT-’255’
STX 02 002 ALT-’2’ / ^B
ADR 31 049 1
Snr 31 049 1
DATA: 44 068 D
30 048 0
31 049 1
30 048 0
35 053 5
41 065 A
53 083 S
50 080 P
49 073 I
52 082 R
41 065 A
54 084 T
45 069 E
30 048 1
30 048 0
38 056 8
32 050 2
33 051 3
30 048 0
30 048 0
30 048 0
30 048 0
30 048 0
30 048 0
30 048 0
41 065 A
30 048 0
32 050 2
31 049 1
30 048 0
45 069 E
30 048 0
30 048 0
31 049 1
30 048 0
32 050 2
30 048 0
32 050 2
45 069 E
31 049 1
ETX 03 003 ALT-’3’ / ^C
CS 32 050 ALT-’50’
Example 2
D0207 Command 'D', Program-Nr. 02, following columns 07
WASH Name: 'WASH'
1082300000000 Plate type 1, Manifold 08, Dispense rate 2, Aspirate
rate 3,
Asp. mode normal/normal (0), Working Mode Plate (0),
Strip Selection no (0), Printer Mode no (0),
fin.asp time NO (00), fin asp. speed NO (00)
W07110400 Wash procedure, Asp. time 07 sec., Dispense pos.
Overfl.(1),
Disp. channel 1, Dispense volume 0400µl
S01010 Soak procedure, Soak time 010 sec, Shake speed 1,
Shake mode low (0)
A0214 Aspirate procedure, Aspirate time 02 sec, Manifold
speed 14mm/s
E0010204 End of Cycle (0), Cycle Nr.01, Repeat Factor 02, Proc.
in Cycle 04
E1 End of Program (1)
(ETX)(CS) End
Resulting Character Sequence

FF 255 ALT-’255’
STX 02 002 ALT-’2’ / ^B
ADR 31 049 1
Snr 31 049 1
DATA: 44 068 D
30 048 0
32 050 2
30 048 0
37 055 7
57 087 W
41 065 A
53 083 S
48 072 H
20 032 ‘‘
20 032 ‘‘
20 032 ‘‘
20 032 ‘‘
31 049 1
30 048 0
38 056 8
32 050 2
33 051 3
30 048 0
30 048 0
30 048 0
30 048 0
30 048 0
30 048 0
30 048 0
57 087 W
30 048 0
37 055 7
31 049 1
31 049 1
30 048 0
34 052 4
30 048 0
30 048 0
53 083 S
30 048 0
31 049 1
30 048 0
31 049 1
30 048 0
41 065 A
30 048 0
32 050 2
31 049 1
34 052 4
45 069 E
30 048 0
30 048 0
31 049 1
30 048 0
32 050 2
30 048 0
34 052 4
45 069 E
31 049 1
ETX 03 003 ALT-’3’ / ^C
CS 02 002 ALT-’2’
Download Plate
PD20028002700150037603200370 Command Plate type 2, Bottom Form
Flat(0),
Bottom aspirate position (0280), Bottom wash position (0270)
Overflow position (0150), Dispense position (0376)
Aspirate position 1 (0320), Aspirate position 2 (0370)
(ETX)(CS) End

FF 255 ALT-’255’
STX 02 002 ALT-’2’ / ^B
ADR 31 049 1
Snr 31 049 1
DATA: 50 080 P
44 068 D
32 050 2
30 048 0
30 048 0
32 050 2
38 056 8
30 048 0
30 048 0
32 050 2
37 055 7
30 048 0
30 048 0
31 049 1
35 053 5
30 048 0
30 048 0
33 051 3
37 055 7
36 054 6
30 048 0
33 051 3
32 050 2
30 048 0
30 048 0
33 051 3
37 055 7
30 048 0
ETX 03 003 ALT-’3’ / ^C
CS 1B 027 ALT-’27’
Download Rinse Parameter

(FF)(STX)(ADR)(SNr.): Start
RD1503: Command Rinse Time 15 sec, Auto Rinse Time 03
hours
(ETX)(CS): End

FF 255 ALT-’255’
STX 02 002 ALT-’2’ / ^B
ADR 31 049 1
Snr 31 049 1
DATA: 52 082 R
44 068 D
31 049 1
35 053 5
30 048 0
33 051 3
ETX 03 003 ALT-’3’ / ^C
CS 10 016 ALT-’16’
3.5 Description of Error Messages

The instrument can display the following error messages:
Standard Instrument
Program not defined
If the selected program has not been defined, the following message is
displayed:
Program not def

exit
Press exit to remove the message and return to standby.
Run XX yyyyyyyy
- + other yes
Select a defined program that is to be performed.

Wrong Manifold
If the selected program requires a different type of manifold than the manifold that
is fitted to the instrument, the following message is displayed:
WRONG MANIFOLD
exit
Press exit to return to the standby mode.

Either exchange the manifold for the required type of manifold or re-program the
procedure for the type of manifold that is fitted.
If the correct type of manifold is fitted, check the setting in the Adjust
menu.
Manifold Arm Error Conditions
The following error conditions can occur with the manifold arm movement system:
1. Manifold arm does not move up and down.
2. Manifold arm continually moves up and down and the following message is
displayed:
Init_Err. cont?
no yes
3. The following message is displayed:
Manif. arm Error !

Exit
When an error occurs, the Service menu can be used to check the various
motors and sensors.
Manifold Arm does not Move Up and Down

This error can be caused by the following conditions:
Cam not fitted on the motor shaft.
Check that the cam is fixed onto the motor shaft.
Motor is defective or no power to the motor
Replace the motor
Replace the power supply board
Replace the main board
Manifold Arm Continually Moves Up and Down
This error can be caused by the following conditions:
Top Position Optical Switch incorrectly positioned
Check that the optical switch is correctly positioned and the positioning disk is in
the center of the optical switch.
Top Position Optical Switch defective
Replace the optical switch.
Plate Carrier Error Conditions
When a plate transport error occurs and the instrument cannot detect the home
position optical switch, the following message is displayed:
Init-Err. cont?
retry yes
The following error conditions can occur with the plate transport system:
1. Plate support not inserted or incorrectly inserted
2. Plate transport motor defective or blocked
3. Home position optical switch defective
When an error occurs the Service menu can be used to check the motors and
sensors.
Plate Support not Correctly Inserted

Check that the plate support is correctly positioned and can be pushed into the
instrument when the instrument is switched off.
If the plate support can not be pushed in, remove the plate support and check the
following:
1. The guide bar is clean and there is nothing that could stop the plate support
moving.
2. Insert the plate support and ensure that it is fitted over the automatic
microplate positioning pin.
Plate Support Correctly Inserted Plate Support Incorrectly Inserted
Microplate Microplate
Positioning Positioning
Pin Pin
The plate support cannot be moved under the manifold arm if it is not fitted
over the pin.
Program too Big
If more than 60 process steps have been defined, the following message is
displayed:
Program too Big

Cont
Press cont to remove the message and re-define the program using less than 60
process steps.
Dispensing Pump Error
If the instrument detects that the dispensing pump is not rotating during a
procedure the following message is displayed:
XXXXXXXXXXXXXXX
Disp.Sens.Err.!!
Where XXXXXXXX is the displayed procedure message.

The instrument is now locked with this error message, switch the instrument off
and then switch it on again.
Change the dispensing pump.
Instrument with Liquid Sensor and Bottle Holder

Waste Bottle Full at START
When the selected program is confirmed, the instrument checks if the waste
bottle is full.
If the waste bottle is full, the following message is displayed:
Wastebottle Full
yes
Press yes to remove the message to return to the start and the following
Run XX yyyyyyyy
- + other yes
Empty the waste bottle and restart the program.

Waste Bottle Full during PROCEDURE
If the waste bottle is full during the washing procedure, the following message is
displayed after the washing procedure is completed:
Wastebottle Full
yes
Press yes to remove the message to proceed to the standby mode. The following
Run XX yyyyyyyy
- + other yes
Empty the waste bottle and the instrument is ready to start the next procedure.
Liquid Bottle Empty
If the liquid bottle is empty before program is started, the following message is
displayed:
Liq. Empty Ch: X

yes
Fill the liquid bottle and press yes to remove the message and continue with the
program.
Waste Sensor Error
This message is displayed only when the Liquid Sensor option is set to yes in the
service menu. The bottle holder should not be connected to the instrument.
Waste Sens Error

yes
Instrument Fitted with Printer

Printer Error
If the protocol can not be printed, the following message is displayed:
Printer Error
cont retry
Check that the printer is connected to the instrument and switched on and that
there is paper in the printer.
When the printer error has been corrected, press retry to repeat the printing
procedure.
If the printout is not required, press cont to return to the standby mode.
Instrument Fitted with Manifold Detect Sensors

Manifold Arm Error
If the manifold arm is not lowered correctly due to the manifold catching on
something. (For example: the side of the wells) The following message is
displayed:
Manif. arm Error !

Exit
Press exit to remove the error message and the instrument returns to the
standby mode.
Check that the microplate is correctly inserted into the plate support.
Check that the manifold arm is not blocked.
Check if the needles are catching on the side of the microplate, use the Adjust
menu to adjust the positions for this type of microplate.
Wrong Manifold
The following message may be displayed when a program is started.
Wrong Manifold
exit
Press exit to remove the error message and the instrument returns to the
standby mode.
Check that the correct manifold is fitted for this program.
If the correct manifold is fitted, use the Service menu to check the manifold type
optical switches are working correctly.
If the Fill Verification option is fitted, check that the ribbon cable is connected
correctly. Connector 1 should be used for 16 manifolds and connector 2 for 8 and
12 manifolds.
Fill Verify Board Screws
Sensor
For an 8 or 12
manifold only
use connector 2
1 2
Instrument Fitted with Fill Verification

The Fill Verification option is a method of checking the filling of the wells in a
microplate. Possible errors with the dispensing system as well as the aspiration
system can be recognised.
The Fill Verification only works with the OVERFLOW wash procedure.
The wash buffer forms an electrical connection between the dispensing needles
and the aspirating needles. As soon as the wash liquid touches the aspirating
needles the electric circuit is closed.
The following errors occur using this method.
• Fill Comm. Error
• Fill Dispens Error
• Fill Aspiration Error
• Fill Function Error
Fill Comm. Err.
If there is no communication between the main board and the fill verification
board, the following message is displayed:
Fill Comm. Err.

Call Service !
Check U14 on the main board and ensure that the fill verification cable is
connected correctly.
Fill Dispens Error
If the instrument detects that any of the needles are dispensing no or too little
solution, the instrument stops and the following error messages are scrolled on
the display:
Inadequate Wash!
exit
Check Disp. Path

exit
Press the exit key to remove the error message and to return to the standby
mode.
Possible errors could be a result of blocked dispensing needles, bubbles in the
dispensing channels, empty liquid bottles, the dispensing pump no longer
functions or a non-conductive liquid is being used.
If no connection between the dispensing needles and the relevant aspirating
needles is made at least 30 µl before the end of the dispensing step, the wash
program will be interrupted.
Check that the dispensing needles are clear and that the reagent bottle has
enough solution and the inlet tube is in the solution.
Use the Prime menu to fill the liquid system with solution.
Fill Aspiration Error

When the well is not correctly aspirated the following error is displayed:
Fill Asp. Err !

exit
The problem is caused by a blocked aspirating needle or the aspirating pump

may not be functioning correctly. Use the cleaning needles to clean the aspirating
needles or replace the pump.
In order to prevent the wells from overflowing, the instrument checks when the
individual aspirating needles come into contact with the wash solution. If this
happens before 200 µl has been dispensed into the wells the program is stopped
immediately. This can occur when the well was not or not completely aspirated,
due to a blocked aspirating needle / tube or the general aspiration being too
weak.
Fill Function Error
If the fill verification board needle status can not be deleted, the following error
Fill Funct.Err.!
exit
Press exit to remove the error message and return to the standby mode.
The problem is caused by an electrical conducting liquid connection from the
dispensing to the aspirating needles on the manifold. Disassemble the manifold
and clean it. Test it using the Service menu/Function Test or change the manifold
if the old manifold does not work.
The prerequisite for a definite identification of the above mentioned error, is the
lack of an electrical connection between the dispensing and aspirating needle(s)
before the wash procedure is started and after the priming procedure. The
instrument checks this continually before the wash procedure is started and at the
beginning of each row. If a connection already exists, the program is interrupted
immediately or will not even be started.
The underside of the manifold must then be cleaned, as the deposits from the
wash buffer can produce an electrical connection between the needles, which
could prevent the Fill Verification option from functioning correctly.
Test Conditions
The following conditions are required for the Fill Verification to function flawlessly.
• The conductance of the wash buffer must be between 5 mS/cm and
30 mS/cm.
• The amount of wash liquid dispensed must be enough so excluding the
Overflow Position at least 100 µl overflow is produced.
• At least 450 µl should be dispensed with an Overflow Position slightly above
the upper well limit.
Conductance of a Standard Wash Buffer

Most of the commercial wash buffers lie in the mentioned conductance ranges.
The conductance is proportional to the wash buffer concentration and can be
changed.
The following values were measured using a normal dilution:
Wash buffer
Ortho Diagnostic System 1:20 14.0 mS/cm
SIGMA .Phosphate buffered saline Tablets 1 tablet in 200ml 14.7 mS/cm
bioMérieux. Phosphat buffer concentrate 1:25 13.7 mS/cm
BOUTY.Beta IgM 1:20 14.2 mS/cm
MUREX .Wash fluid (Glycine/Borate) 1:20 7.8 mS/cm
BIOTEST ELPHA . ( Nr. 3+4) 1:10 28.1 mS/cm
4. Removing and Replacing

the Instruments Cover
4.1 Removal of Instruments Top Cover
Before the manifold arm movement system can be serviced, the instrument's top
cover must be removed.
The instrument cover is removed using the following procedure:
1. Switch the instrument off and remove the mains power cable from the mains
power connector.
2. Loosen and remove the six screws on the sides of the instrument.
Screws Screws
3. Raise the manifold arm so that it is in the fully open position.

4. Carefully tilt the plate support and pull the plate support away from the
instrument.
Guide Bar
Screws
Cam
5. Loosen and remove the two guide bar screws and remove the guide bar
from the instrument.
6. Pull the cam off the motor shaft.
7. Loosen and remove the three cover screws on the right hand side of the
manifold arm.
Screws
8. Carefully lift the cover a small distance away from the instrument.
9. Either place the cover by the side of the instrument or disconnect the
keyboard/display connector from the main board.
4.2 Replacing the Instruments Top Cover

The instrument's top cover is replaced using the following procedure:
1. Reconnect the keyboard / display connector, if it was removed.
3. Carefully slide the cover over the instrument.
4. Insert and tighten the three cover screws on the right hand side of the
manifold arm.
5. Replace the guide bar and insert and tighten the two guide bar screws.
6. Slide the cam onto the motor shaft. The shaft has a flat area so that the cam
can not be incorrectly fitted.
Cam Wheel
7. Insert and tighten the six cover screws on the side of the instrument.
8. Slightly tilt the plate support towards the right.
Plate Support
The plate support must be slightly tilted so that it goes over the pin
that activates the automatic microplate centering mechanism.
9. Carefully slide the plate support with the waste sink at the rear over the
guide bar.
10. Push the plate support so that it is fully placed in the instrument.
11. Lower the plate support.
12. Lower the manifold arm.
13. Insert the mains power cable and switch the instrument on.
5. Liquid System
5. Liquid System
5.1 Introduction
This chapter contains the description of the liquid system of the instrument.
The liquid system consists of the following parts depending on the various
instrument options:
Standard Instrument
Dispensing pump and solenoid valves for dispensing liquid into the microplate.
Aspirating pump for aspirating the liquids out of the microplate.
Instrument Options
Aerosol pump and special manifold to aspirate any aerosol vapors away from the
microplate when it washed.
Waste bottle full sensor to detect when the waste bottle is full.
Fill Verification manifold to detect that each channel is dispensing liquid.
5. Liquid System
5.1.1 Liquid System Diagram

Standard Instrument *
Manifold Dispense
Marked blue Solenoid Valve
Dispensing Pump
Marked red
Prime Valve
Marked
red
Aspirating Pump
Channel select
Solenoid Valves
Instrument with Aerosol Aspiration *

Dispense Solenoid
Manifold Valve
Marked blue
Marked red Dispensing Pump
Prime Valve
Marked yellow
Marked
red
Aspirating Pump
Marked
yellow
Channel select
Solenoid Valves
Aerosol Pump
5. Liquid System
5.1.2 Dispensing System Description

The instrument can use up to four different solutions depending on the number of
solenoid valves that are fitted.

inlet inlet inlet inlet
Prime
Prime
Valve
Valve
Waste Tube Aerosol Tube Waste Tube
When the solution is to be dispensed, the instrument activates the solenoid valve
for the required channel, starts the dispensing pump and activates the dispense
solenoid valve.
Instruments with Pinch Valves
Tubing to
Prime
Dispensing Pump input
Valve
Instruments with Solenoid Valves.
Aerosol Tube Hole Waste Tube Hole
Some of the instruments may include the prime valve.
5. Liquid System
5.2 Replacement Procedure

5.2.1 Replacing the Dispensing Pump
The dispensing pump is replaced using the following procedure:
1. Disconnect the instrument from the power supply and remove the cover.
2. Remove the dispensing pump power connector from the main board.
3. Turn the instrument onto the right hand side.
Waste Bottle
Dispensing
Pump
Connected to
TOP Connector
Waste Tube
Aspirating Tube to Manifold Aspirating Pump
4. Disconnect the tubing from the dispensing pump.

5. Carefully pull the dispensing pump away from the instrument.
The pump is held in place by three screws.
Dispensing
Pump
6. Carefully pull cable and connector through the slot in the middle plate.
7. Remove the dispensing pump.
8. Exchange the dispensing pump.
9. Insert the cable and connector through the slot in the middle plate.
10. Carefully place the dispensing pump into position and secure it with the
three screws.
5. Liquid System
11. Reconnect the tubing to the pump

Pump Pump
Inlet Outlet
12. Reconnect the dispensing pump power connector onto the main board.
13. Replace the instrument's cover.
When the dispensing pump is replaced, perform Clear All in the service
menu. The dispensing pump calibration must be performed. Ensure that
the calibration procedure is repeated if the weight (300 µl) is greater than
33 g or less than 24 g before calibrating the instrument for the first time.
Reset all features of the instrument (Plate Type, Customers specific

programs, Manifold Type, Priming position, Adj. Plate. Ref, Options and
select number of liquid channels).
5. Liquid System
5.2.2 Replacing the Solution Solenoid Valves

(Instruments with Pinch Valves)
The solution solenoid valves are replaced using the following procedure:
1. Disconnect the instrument from the power supply.
2. Remove the inlet tubing connector from the holding plate above the solenoid
valves.
3. On the end of the solenoid valve, press and hold the white center piece
down and carefully remove the tubing from the pinch valve.
Tubing
Electrical Block
Pinch Valve Clip
Spacer
4. Remove the clip on the end of the shaft behind the electrical block.
5. Remove the electrical block from the shaft.
6. Disconnect the connectors from the electrical block.
7. Remove the two screws that hold the pinch valve unit onto the back panel.
8. Carefully remove the electrical block from the instrument.
9. Exchange the electrical block.
10. Connect the connectors onto the electrical block.
11. Exchange the Pinch valve unit.
12. Screw the new pinch valve unit onto the back panel.
13. Slide the electrical block over the shaft for the pinch valve.
Ensure that the spacer is fitted between the pinch valve and the electrical
block.
14. Slide the clip onto the end of the shaft behind the electrical block.
15. Press and hold the white center piece down on the end of the solenoid valve
and carefully insert the tubing into the pinch valve.
16. Insert the inlet tubing connector into the holding plate above the solenoid
valves.
17. Adjust the tubing so that it is not bent.
5. Liquid System
5.2.3 Replacing the Solution Solenoid Valves

The solution solenoid valves are replaced using the following procedure:
2. Remove the excenter from the motor axis.
3. Remove the plate transport.
4. Remove the plate transport bar by unscrewing the two screws.
Valves
Valves
CPU cable Screw holes
5. Remove the housing by unscrewing the nine screws.

Ensure that the cable connecting the display to the CPU is disconnected
first!
6. Disconnect the solenoid cable from the CPU.
7. Remove the inlet and the outlet tubing from the valve block.
8. Unscrew the two screws securing the valve block on to the instrument.
Screws
Inlet solenoid valves
9. Remove the complete valve block from the instrument.
5. Liquid System
10. Position the new valve block into the instrument and secure it into place by
tightening the two screws.
11. Replace the inlet and the outlet tubing into the valve block.
12. Connect the solenoid cable to the CPU.
13. Replace the housing and secure it with the nine screws.
Ensure that the cable connecting the display to the CPU does not get
trapped!
14. Replace the plate transport bar and secure it by tightening the two screws.
15. Replace the excenter.
16. Connect the instrument to the power supply.
5.2.4 Replacing the Dispense Solenoid Valve

The dispense solenoid valve is replaced using the following procedure:
3. Remove the two screws that hold the solenoid valve onto the bottom plate.
Waste Bottle
Dispensing
Pump
Connected to
TOP Connector
Waste Tube
Aspirating Tube to Manifold Aspirating Pump
screws
4. Carefully remove the solenoid valve a small distance away from the
instrument.
5. Disconnect the electrical connectors.
6. Disconnect the tubing from the connectors.
7. Exchange the solenoid valve.
8. Reconnect the tubing onto the solenoid valve.
9. Reconnect the electrical connectors.
10. Screw the new solenoid valve into place on the bottom plate.
5. Liquid System
5.2.5 Replacing the Aspirating Pump

The aspirating pump is replaced using the following procedure:
2. Remove the aspirating pump connector from the main board.
3. Disconnect the tubing from the aspirating pump.
5. Loosen and remove the three screws that hold the aspirating pump onto the
bottom plate.
Waste Bottle
Dispensing
Pump
screws
Connected to
TOP Connector
Aspirating
Waste Tube
Pump
6. Carefully pull the pump cables and connector through the cable slot in the
middle panel.
7. Carefully remove the aspirating pump from the instrument.
8. Exchange the aspirating pump.
9. Carefully push the pump cables and connector through the cable slot in the
middle panel.
10. Carefully insert the aspirating pump into position and screw it into place with
the three screws.
11. Ensure that the three rubber mounting blocks are fitted.
Rubber Mounting
Blocks
Pump Pump
Outlet Inlet
12. Reconnect the aspirating pump connector onto the main board.
13. Reconnect the tubing.
14. Replace the instrument's cover. (See chapter 4)
5. Liquid System
5.2.6 Replacing the Aerosol Pump

The aerosol pump is replaced using the following procedure:
2. Remove the aerosol pump connector from the main board.
3. Disconnect the tubing from the aerosol pump.
5. Loosen and remove the three screws that hold the aerosol pump onto the
bottom plate.
Waste Bottle
Aerosol
Dispensing Pump
Aerosol Bottle Pump
screws
Connected to
TOP Connector
Aerosol tube to Manifold

Aspirating
Waste Tube
Pump
6. Carefully pull the pump cables and connector through the cable slot in the
middle panel.
7. Carefully remove the aerosol pump from the instrument.
8. Exchange the aerosol pump.
9. Carefully push the pump cables and connector through the cable slot in the
middle panel.
10. Carefully insert the aerosol pump into position and screw it into place with
the three screws.
11. Ensure that the three rubber mounting blocks are fitted.
Rubber Mounting Blocks
Pump Outlet Pump Inlet
12. Reconnect the aerosol pump connector onto the main board.
13. Reconnect the tubing.
14. Replace the instrument's cover. (See chapter 4)
6.1 Manifold Arm Movement System
This option contains the description of the movement system for raising and
lowering the manifold arm.
The manifold arm is raised and lowered by a cam, which is rotated by a motor.
The manifold arm is kept in the raised position, when the power is removed from
the instrument, by a spring.
The top position is detected by an optical switch and a slot in the positioning disk.
If the Manifold Detect option is fitted, a sensor on the manifold arm detects when
the manifold arm is not lowered correctly due to an error. For example: if the
needles are incorrectly positioned and touch the microplate.
Bottom Position
Optical Switch
Sensor
Manifold
Cam
Top Position
Manifold
Optical Switch
Sensor
Cam
Error Condition
Manifold
Optical Switch
Sensor
Cam
Microplate
6.1.1 Description of Manifold Arm Movement Parts

The drawings below show the main components for the manifold arm movement
system.
Front View Top View
Top Position
Optical Switch
Top Position
Positioning
Optical Switch
Positioning Disk
Disk
Motor Motor
Cam
Cam
Spring
Spring
6.1.2 Adjusting the Manifold

Position the reference plate and remove the black cam wheel. Position the
adapter plate manifold so that when the manifold is lowered, the aspirating
needles are above the crosshairs on the reference plate. Secure the screws with
adhesive.
Screws Adjust the manifold so that

when the manifold is in the
lowered position, the
aspirating needles are in
the middle of the crosshair
on the adjustment plate
6.2 Plate Transport System

This option contains the description of the plate transport system for moving the
plate support in and out of the instrument.
The plate support is moved by a motor, which drives a gear wheel that fits in the
toothed rack on the bottom of the plate support.
The home position is detected by an optical switch and an edge on the plate
support.
The plate support is supported by the guide bar on the right hand side and a
series of support pins on the left-hand side.
Front View
Waste Sink
Guide Bar Home Position

Optical Switch
Plate Support
Motor
Support Screw
Gear Wheel
Instrument Frame
Top View Bottom View

Guide Bar
Home Position
Edge
Home Position
Rack Optical Switch
Motor
Gear Wheel
Motor
Gear Wheel
Home Position Guide Bar

Optical Switch
6.2.1 Plate Support

The plate support contains an automatic plate positioning system so that the
microplate is always correctly positioned.
Microplat
Guide e
Positionin
Bar g Plate
.
Waste
Sink
Hom
Positio
e
Microplat n Edg
e
Positionin e
g
Plat
e Sprin
g
Rac
k
6.2.2 Leveling the Plate Support

In order that all the wells in the microplate are correctly aspirated, it is important
that the plate support is level with the manifold.
The plate support is leveled using the following procedure:
1. Insert the plate support and push it back so that it positioned over the two
middle support screws.
1 2 3 4
2. Using a strip of tape, temporarily stick the plate mismatch sensor in the top
position.
3. Carefully lower the manifold arm so that the manifold needles touch the
plate support.
4. Check that all the needles touch the plate support.
Manifold
Plate Support
Guide Bar
Support Screws
5. If all the needles do not touch the plate support, adjust the height of the
second support screw until all the needles touch the plate support.
The support screws are locked in place with screw retaining liquid.
6. Raise the manifold arm to the fully back position.
7. Push the plate support in until it is over the first support screw.
8. Adjust the height of the first support screw until it just touches the plate
support.
9. Push the plate support backwards and forwards to check that it can move
over the first and second support screws.
10. Push the plate support in until it is over the third support screw.
11. Adjust the height of the third support screw until it just touches the plate
support.
freely over the support screws.
13. Push the plate support in until it is over the fourth support screw.
14. Adjust the height of the fourth support screw until it just touches the plate
support.
freely over all the support screws.
Lock the support screws into place with screw retaining liquid.
16. Remove the plate support.
17. Remove the guide bar.
6.2.3 Microplate Positioning System

The microplate positioning system automatically positions the microplates
correctly in the plate support.
The pin below the plate support (when it is in the home position) pushes the
microplate positioning plate back so that the microplate can be inserted.
Microplate
Spring Positioning
Pin
When the plate support moves away from the home position, the spring pulls the
microplate positioning plate back and the plate then automatically positions the
microplate.
Microplate
Positioning
Pin
When the plate support is returned to the home position the pin pushes the plate
back so that the microplate is released and can be removed.
6.3 Replacement Procedure

6.3.1 Replacing the Manifold Arm Error Sensor
The metallic cover is replaced using the following procedure:
power connector.
3. Disconnect the tubing from the manifold.
4. Remove the two screws that hold the manifold in place.
5. Remove the manifold from the instrument.
6. Remove the two screws that hold the sensor onto the cover of the manifold
arm.
Screws
Manifold Detection Board
Sensor
7. Exchange the sensor and secure it onto the cover.

8. Replace the manifold and secure it into place with the two screws.
Ensure that the manifold type indicator is correctly positioned in the front
optical switches.
9. Reconnect the tubing onto the manifold.
10. Lower the manifold arm to the normal position.
11. Reconnect the instrument to the mains power supply and switch the
instrument on.
12. Use the Service menu to check that the optical switch is working correctly.
6.3.2 Replacing the Motor

The manifold arm motor is replaced using the following procedure:
2. Remove the spring from the pin on the positioning disk.
3. Remove the motor connector from the main board.
4. Remove the two screws that hold the motor onto the middle panel of the
instrument.
5. Carefully remove the motor from the instrument, ensure that the optical
switch is not damaged when the motor is removed.
Top Position
Optical Switch
Positioning
Disk
Screws
Motor
Spring
6. Exchange the motor with the positioning disk (positioning disk is glued onto
the motor shaft).
7. Screw the motor into place on the middle panel of the instrument.
Position the motor so that the cables are on the right hand side.
8. Connect the motor connector onto the main board.
9. Connect the spring between the pin on the positioning disk and the bracket
on the middle panel.
6.3.3 Replacing the Top Position Optical Switch

The top position optical switch is used to detect when the manifold arm is in the
top position.
The top position optical switch is replaced using the following procedure:
2. Disconnect the optical switch connector from the main board.
3. Loosen and remove the screw that holds the optical switch onto the sensor
holding plate.
Top Position Top Position
Optical Switch Screw Optical Switch
Screw
4. Exchange the optical switch.

5. Screw the new optical switch onto the sensor holding plate, ensure that the
positioning disk is in the middle of the optical switch.
6. Connect the optical switch onto the main board.
6.3.4 Replacing the Plate Support Home Position Optical

Switch
The home position optical switch is replaced using the following procedure:
(See chapter 4).
2. Disconnect the connector for the optical switch from the main board.
3. Remove the screw that holds the optical switch onto the middle plate.
Detector gate
Support
4. Carefully pull the connector through the hole in the middle plate.
5. Exchange the optical switch.
6. Carefully insert the connector for the new optical switch through the hole in
the middle plate.
7. Insert the optical switch on the middle plate and screw it into place.
Ensure that the spacer is placed between the optical switch and the middle
plate and that the optical switch is vertical.
8. Secure the connector for the optical switch onto the main board.
9. Replace the instrument's cover. (See chapter 4).
10. Switch the instrument on and use the Service menu to check that the sensor
is working correctly.
11. Use the Adjust menu to set the parameters for the plate definitions as these
could be slightly different with the new optical switch.
12. If a 16 way manifold is used, use the Service menu to set the Prime and
Rinse positions as these could be slightly different with the new optical
switch.
7.1 Introduction
This chapter gives the replacement and adjustment procedures for the various
electronic parts of the instrument.
This chapter also contains the connection diagrams that show how the various
parts of the instrument are interconnected.
The instrument contains the following electronic parts:
1. Main Board
This is the major board in the instrument and contains the microprocessor
and the EPROM.
2. Power Supply Board
This board is used to supply the various voltages that are required by the
instrument.
3. Keyboard and Display Unit
The keyboard and display unit is used to communicate with the instrument.
4. Other Switches
This section gives the layout and circuit diagrams for the other switches that
are used in the instrument.
7.2 Interconnection Diagram

The diagram below shows how the various parts of the instrument are
interconnected.
Dispensing Pump
Fill Verify and
Aspirating Pump Plate Mismatch
Board
Solenoid 4
Manifold Typeand
Aerosol Pump Plate Transport Manifold Arm Plate MismatchBoard Solenoid 3
Motor Motor
Solenoid 2
Plate Home Position
Optical Switch
Home Position Solenoid 1
Optical Switch Liquid
Dispense
Detection
Solenoid
System
Orbital Shaking
Motor *
Printer / Serial
Connector
EEPROM
CPU
Jumper x 100
RS232 (open)
RS485 (closed)
Barcode Reader
Keyboard /
Display Unit
Power Supply Board
Fuses
Mains Input
ON/OFF Switch
* The Orbital Shaking option is not used.
7.3 Main Board

The diagram below shows the main board:
Printer / RS 232 Liquid Detector Barcode Reader
Connector Connector Connector
Jumper x 100
RS232 (open)
RS485 (closed)
Fuse 4A / 250V
Quick Blow Solenoid Valves
Connector
Manifold Arm
Motor Connector
Adjust
Dispensing
Speed
Plate Support
Motor Connector
Dispensing Pump
Connector
Orbital Shaking
Motor Connector
Power Supply
Connector Manifold Detect
Fill Verify Connector
Manifold Arm Home

Position Sensor
CPU Plate Support Home

Position Sensor
Orbit Shaking Home

Position Sensor
Orbit Shaking
Slotted Disk Sensor
EEPROM
Keyboard/Display
Connector
Aerosol Pump
Connector
Aspirate Pump
Connector
Battery Jumper Battery
Reprogramming the Instrument

The instrument is fitted with an EEPROM (Electrical Erasable Programmable
Read Only Memory) which can be reprogrammed for new software versions or
software versions in different languages using the serial interface and a
computer.
It is now NOT necessary to exchange the chip as with the earlier versions of the
instrument which where fitted with an EPROM (Erasable Programmable Read
Only Memory).
7.3.1 Replacing the Main Board

The Main Board is replaced using the following procedure:
1. Disconnect the instrument from the power supply and remove the top cover.
(See chapter 4).
2. Disconnect ALL the connectors from the main board.
Ensure that the connectors are identified so they can be reconnected
correctly
3. Turn the instrument onto the left-hand side.
4. From the bottom of the instrument, remove the nine screws that hold the
main board onto the bottom plate.
Main Board Screws
5. Carefully remove the main board from the instrument.
6. Exchange the main board.

Ensure that the new main board contains all the required chips for the
various instrument options.
Printer Option Chip
EEPROM
CPU
Barcode Option Orbital Shaking Fill Verification
Chips Option Chip Option Chip
7. Replace the new main board into the instrument and screw it in place with
the nine screws. (Ensure that the battery jumper is set).
8. Reconnect all the connectors to the main board.
9. Replace the instrument's top cover.
instrument on.
7.3.2 Replacing the Battery

1. Remove the main board from the instrument.
2. Replace the battery using the solder iron.
3. Replace the main board.
4. Reconnect all connectors.
instrument on.
When the main board or the battery is replaced, perform Clear All in the
service menu. The instrument must be calibrated for the dispensing pump
and for the manifolds. Ensure that the calibration procedure is repeated if
the weight (300 µl) is greater than 33 g or less than 24 g before calibrating
the instrument for the first time.
Reset all features of the instrument (Plate Type, Customers specific programs,
Manifold Type, Priming position, Adj. Plate. Ref, Options and select number of
liquid channels).
7.4 Power Supply Board

The power supply board supplies all the required voltages for the instrument.
Fuse 2 A/250V
Quick Blow
Mains Power
Output Voltages
Pin
Output Voltage
Definition Red
+24 Volt Cable Connector
Black
GND
Green
7.4.1 Replacing the Power Supply Board

The power supply board is replaced using the following procedure:
1. Disconnect the instrument from the power supply and remove the top cover.
2. Remove the two screws that hold the power supply board front cover in
place.
3. Remove the two screws that hold the rear of the power supply board in
place.
4. Carefully pull the power supply board a small distance out of the instrument.
5. Disconnect the mains input cable from the power supply board.
6. Disconnect the output voltage cable from the power supply board.
7. Remove the power supply board.
8. Remove the four screws that hold the spacing blocks onto the power supply
board.
9. Exchange the power supply board.
10. Screw the four spacing blocks onto the new power supply board.
11. Connect the mains input cable to the power supply board.
12. Connect the output voltage cable to the power supply board.
13. Carefully fit the new power supply board into place and screw into place with
the two rear screws.
14. Replace the cover for the power supply board and screw it into place.
15. Replace the instrument's top cover. (See chapter 4)
instrument on.
7.5 Keyboard and Display Unit

The keyboard and display unit are not repairable.
Display Unit
The display unit is a Liquid Crystal display unit with two rows of sixteen digits.
Display Unit Front View Display Unit Back View
Adjustment
potentiometer
Keyboard cable Power ON LED

connector
Ribbon cable from
Main Board
Keyboard
The keyboard is a membrane keyboard with four keys.
Keyboard View Circuit Diagram
Key 1 Key 2 Key 3 Key 4
POWER
Display Contrast Adjustment

The potentiometer on the display board is used to adjust the contrast for the LCD
display unit.
To adjust the display contrast, turn the potentiometer.
When the potentiometer is turned clockwise, the contrast is increased.
When the potentiometer is turned anticlockwise, the contrast is decreased.
Adjust potentiometer until the displayed messages can easily be read but the
blocks that make up each segment can not be seen.
Correct Too Dark Too Light

T E S T T E S T T E S T
7.5.1 Replacing the Keyboard or Display Unit

The keyboard and display units are fitted to the instrument's top cover and
replaced using the following procedures:
2. Disconnect the keyboard/display connector from the main board.
Replacing the Display Unit

The display unit is held onto the bottom side of the instrument's top cover by four
screws.
Screws
To replace the display unit, use the following procedure:

1. Remove the keyboard ribbon cable from the connector on the display unit.
2. Remove the four screws that hold the display unit in place.
3. Carefully lift the display unit a small distance away from the top cover.
4. If the keyboard function is correct, carefully unsolder the cables of the power
On LED from the display unit board.
5. If the keyboard is defective or damaged, remove the keyboard from the
instrument.
6. When the keyboard has been removed, push the power On LED through the
hole in the instrument cover and side the cables to the larger hole and pull
the LED through the larger hole.
7. Disconnect the ribbon cable from the display unit.
8. Exchange the display unit.
9. If the LED was removed, push the LED through the large hole and slide the
cables to the small hole and push the LED into the small hole.
10. Reconnect the ribbon cable onto the display unit.
11. Screw the display unit loosely into place with the four screws.
12. If the LED was not removed, carefully solder the LED cables onto the
display unit board. (red = + left contact, black = - right contact)
- contact
Black Wire
+ contact
Red Wire
13. Carefully turn the instrument's top cover over and check that the display unit
is correctly positioned in the center of the window of the keyboard.
If the display unit is not centered, slide the display unit until it is centered.
14. Carefully turn the instrument's top cover over and tighten the four screws
that hold the display unit into place.
15. Reconnect the keyboard ribbon cable.
instrument on.
Replacing the Keyboard

Use the following procedure to replace the keyboard:
1. Disconnect the keyboard ribbon cable from the display unit.
2. Then peel the old keyboard off the instrument's top cover.
3. Carefully pull the ribbon cable through the slot in the instrument's top cover.
4. Slide the ribbon cable for the new keyboard through the slot in the
instrument's top cover.
5. Connect the ribbon cable onto the display unit.
6. Remove the backing paper from the back of the keyboard and carefully stick
the keyboard into place on the instrument's top cover.
instrument on.
7.6 Manifold Detect Board

The manifold detect board is used to detect the type of manifold that is fitted to
the instrument and if it is being lowered correctly.
Board Layout
Manifold Type Plate Mismatch
Optical Switch
Connector
Optical Switches
U1 U2 U3
R3
R1
R2
Optical Switch Circuit Diagram

R1
+5V
1
GND
3
Signal
2
7.6.1 Replacing the Manifold Detect Board

The manifold detect board is replaced using the following procedure:
power connector.
3. Disconnect the tubing from the manifold.
6. Remove the two screws that hold the board in place.
Manifold Detection Board Screws
Sensor
7. Disconnect the ribbon cable from the manifold detect board.

8. Carefully remove the manifold detect board from under the sensor.
9. Exchange the manifold detect board.
10. Connect the ribbon cable to the manifold detect board.
11. Carefully place the manifold detect board under the sensor.
12. Screw the manifold detect board into place.
Ensure that the earth cable is screwed under one of the screws.
13. Replace the manifold and screw it into place with the two screws.
Ensure that the manifold type indicator is correctly positioned in the front
optical switches.
14. Reconnect the tubing onto the manifold.
instrument on.
7.7 Fill Verification Board

The Fill Verification board is used to verify that the manifold is dispensing liquid
through all the dispensing needles. The board also automatically detects the type
of manifold fitted and if the manifold arm is being lowered correctly.
Plate Mismatch
Optical Switch
Connectors 1
for Manifold 2
8 and 12
manifold
only use
connector 2
Connector
This board is not repairable.
7.7.1 Replacing the Fill Verification Board

The Fill Verification board is replaced using the following procedure:
1. Switch the instrument off and remove the mains power cable from the
connector.
3. Disconnect the manifold tubing from the quick connectors.
4. Remove the screws that hold the cover plate in place and remove the cover
plate.
Cover Plate Screw

Quick Connectors
5. Disconnect the manifold ribbon cables from the board.

8. Remove the three screws that hold the board in place.
Fill Verify Board Screws
Sensor
For an 8 or 12
manifold only
use connector 2
1 2
9. Disconnect the ribbon cable from the Fill Verification board.

10. Carefully remove the Fill Verification board from under the sensor and
exchange it.
11. Connect the ribbon cable to the Fill Verification board.
12. Carefully place the Fill Verification board under the sensor.
13. Screw the Fill Verification board into place.
Ensure that the earth cable is screwed under one of the screws.
14. Replace the manifold and screw it into place with the two screws.
15. Connect the ribbon cables from the manifold into the connectors on the
board.
16. Reconnect the manifold tubing onto the quick connectors.
17. Replace the cover plate and screw it into place.
instrument on.
7.8 Optical Switches

The instrument uses the following optical switches:
• Transport home position
• Manifold arm top position
• Orbital shaking home position
• Orbital shaking step counter
Switch Layout
Circuit Diagram
A
E D
K
G RD
O UT
8. Serial Interface
8. Serial Interface
8.1 Hardware Specifications
The instrument is controlled by the computer and the communication between the
computer and the instrument is done through an RS-232-C interface.
The interface specification may be either to the CCITT interface standard or to
the EIA RS-232-C interface standard.
8.1.1 Pin Designation

The illustration below shows the pin assignment of the DB 9 connector fitted to
the instrument.
Pin 5 Pin 1
Pin 9 Pin 6
All connected devices must be approved and listed as per EN 60950, UL 1950 or
CSA C22.2 No. 950 for Data Processing Devices
8. Serial Interface
8.1.2 RS-232-C Interface Lines

The Serial Interface of the instrument is connected to a start-stop synchronized
serial RS-232-C circuit.
The list below names the interface lines of the instrument's 9 pin connector.
PIN Number DESCRIPTION
1 RS 485 IN/OUT (Option)
2 (RS232) TD Transmit Data
3 (RS232) RD Receive Data
4 RS 485 IN/OUT
5 GND Ground
6 RS-config: if connected to GND RS 485, if open RS 232
is active (only if jumper x100 on the mainboard is open!)
7 No connection
8 RS232 CTS Clear To Send
9 +24V INPUT (Fused 4AF)
The connecting cable used to connect the instrument to the computer should be
wired as given below:
INSTRUMENT COMPUTER
TD connected to RD
RD connected to TD
GND connected to GND
RTS connected to CTS
DSR connected to DCD and DTR
Use the computer handbook to find the correct pin connections.
Instrument Computer 25 pin Instrument Computer 9 pin

1 1 1 1
2 2 2 2
3 3 3 3
4 4 4 4
5 5 5 5
6 6 6 6
7 7 7 7
8 8 8 8
9 20 9 9
8. Serial Interface
8.1.3 Synchronization and Data Format

For the instrument to communicate with the computer correctly, the instrument
and the computer must be set for the same communication parameters.
Check that following parameters are set correctly on both the computer and the
instrument:
1. Start bit length: 1 Bit
2. Stop bit length: 1 Bit
3. Data format: 8 Bits
4. Parity bit: None
5. Baud Rate: 9600 Baud
6. Mode: half duplex
8.1.4 Daisy Chaining Instruments

The instrument can be connected to a computer, which controls a number of
instruments using a Daisy Chain cable. (RS 485 version only).
Da isy Cha in Ca b le
Co m p uter
Ad d re ss #30
Wa sher 1 Wa sher 2 Wa sher 3

Ad d re ss #31 Ad d re ss #32 Ad d re ss #33
Each of the instruments must be defined with a different device address

Each instrument can be controlled by sending the commands to the required
device address.
To select a different device address, start the Remote Mode with the default
device address (#31) and send the command to X 37 (to select the device
address #37).
9.1 Instrument Options
The instrument can be fitted with the following options.
Please note that some of the options listed below require an instrument firmware
level of 3.x. and 4.x.
Barcode Pen HBCS-A300 pen (Hewlett Packard) or a compatible pen
with a magnetic core for reading the microplate
identification numbers for the printout of the washing
protocol.
Included in the HBCS-A300 pen is the Decoder IC: HBCR
1611 (Hewlett Packard)
This decoder can read:
Code 39, Interleaved 2 of 5, UPC A, E0, E1
EAN / JAN 8, 13
Codabar, Code 128
Printer External printer for printing the washing protocol.
We recommend a DPU - 201 GS hand held thermal
printer (or EPSON compatible) with 27 character per line
and a power supply.
RS 232 interface, 9600 Baud, 8 data, 1 stop, no parity and
hardware handshake (BUSY on pin8 on the Washer
connector).
Fill Verification This option checks whether the wash buffer was
dispensed into each well during an overflow wash step
(special manifold required, M8 or M16).
The Fill Verification includes also the Manifold Detection
function.
Note
The Fill Verification Option cannot be combined with the Aerosol
Protection option. Not available for 384 Well Manifold. Possible from
firmware level 3.23 onwards. Factory installation required!
Manifold Automatic detection for the type of manifold fitted:

Detection
For 96 Well plate: M8, M12, M16.
For 384 Well plate: only M32.
Aerosol Special manifold and pump to aspirate any aerosol vapors
Protection away from the microplate when it is washed.
Note
The Aerosol Protection option can not be combined with the Fill Verification
option. Possible from firmware level 3.23 onwards. Factory installation
required! Not available for 384 Well Manifold.
Liquid Channel Option to upgrade the number of liquid channels from 1 to

Upgrade a maximum of 4.
Liquid Sensor Allows the level of liquid in the bottles (wash buffer, rinse
System solution, waste and so on) to be controlled. An acoustic
signal alerts the user when the bottles are empty or full.
Columbus PC Consisting of WinWash and Download including the
Software operating manual and RS232 interface cable.
Firmware Update Consisting of the Download software, the latest version of
the firmware 3.x, as well as 5 language software files
(English, German, French, Spanish and Italian) that can
change the on-board language of the Columbus (from
firmware level 2.23 onwards) in the field.
Drip Mode Drip Mode allows a wash buffer to be added drop by drop.
(Cell Wash) (for example: for Cell Washing)
Note
This option is only available together with our cell wash manifolds
(M 8, M12, M16). Dispensing in Drip mode is possible with the 384 Well
Manifold. Overflow washing using Drip mode is not possible with the 384 Well
Manifold.
384 Well Manifold This option is used for washing the 384 well microplate in
(starting at 4.x) rows and can only be performed with a 32 way Manifold.
9.2 Liquid Detection Option

The liquid detection option is fitted to the instrument using the following
procedure:
1. Place the liquid detection unit onto the bench.
2. Place the liquid bottles onto the correct positions on the liquid detection unit.
3. Place the waste bottle onto the liquid detection unit.
4. Connect the liquid detection unit to the instrument using the supplied cable.
Channel 2 Channel 3 Channel 4

Inlet Inlet Inlet
Mains Power Channel 1
Socket Inlet
Fuse
Compartment
ON/OFF
Switch

Reader each bottle Serial RS 232 for (yellow) (red)
Remote Control
5. To set the instrument so that the Liquid Detection option is used, the option
must be activated using the Set Options option in the Service menu.
9.3 Barcode Reader Option

The Barcode Reader option is fitted to the instrument using the following
procedure:
power connector.
2. Remove the instrument cover. (See chapter 4)
3. Insert the supplied chips into the required holders on the main board.
Barcode Option
Chips
4. Ensure that the chips are correctly orientated.

5. Replace the instrument's top cover. (See chapter 4)
6. Connect the barcode reader into the appropriate socket in the back panel of
the instrument.
Hand Held Barcode

Reader
7. Switch the instrument on.

8. To set the instrument so that the Barcode option is used, the option must be
activated using the Set Options option in the Service menu.
9.4 Printer Option

The Printer option is fitted to the instrument using the following procedure:
power connector.
3. Insert the supplied clock chip into the required holder on the main board.
Clock Chip for Printer O ption
EEPROM
CPU
Ensure that the chip is correctly orientated.
4. Replace the instrument's top cover. (See chapter 4).
5. Plug the printer cable into the appropriate socket in the back panel of the
instrument.
Printer Cable
6. Connect the printer to the instrument using the supplied cable.

7. Connect the power supply cable to the printer.
Power Cable
ON /OFF
Switch
POWER OFF 1 2
ON
Connection Cable
BATT. LOW
PAPER
POWER FEED

9. To set the instrument so that the Printer option is used, the option must be
activated using the Set Options option in the Service menu.
10. Use the Date and Time options in the Service menu to set the correct date
and time.
9.5 Manifold Detection Option

The Manifold Detection option is fitted to the instrument using the following
procedure:
power connector.
3. Disconnect the manifold tubing from the quick connectors.
4. Remove the screw that holds the cover plate in place and remove the cover
plate.
Cover Plate Screw

Quick Connectors

7. Screw the manifold detection board onto the manifold arm with two screws.
Manifold Detection
Board
Senso
r
8. Turn the instrument on the right hand side.

9. Carefully feed the supplied ribbon cable through the instrument.
Slot it between the arm support and plate and then through the large slot in
the middle plate.
Ensure that the cable does not block the movement of the manifold
arm.
10. Connect the ribbon cable onto the manifold detection board and screw the
earth cable under one of the holding screws.
11. Connect the other end of the ribbon cable onto the connector on the main
board.
EEPROM
CPU
Ribbon cable connector
for manifold detection board
12. Reconnect the manifold tubing onto the quick connectors.

13. Replace the cover plate and screw it into place.
14. Replace the instrument's top cover. (See chapter 4).
16. To set the instrument so that the Manifold Detection option is used, the
option must be activated using the Set Options option in the Service menu.
9.6 Fill Verification Option

This option can only be installed by Tecan Austria.
9.7 Aerosol Pump Option

This option can only be installed by Tecan Austria.
9.8 Activating the Options

When the required option has been fitted, the instrument must be configured for
the option using the Select Options in the Service menu. For more information,
see chapter 3.2.8.
10. Maintenance
10. Maintenance
10.1 Cleaning Maintenance
10.1.1 Cleaning the Manifold
The manifold should be removed and thoroughly cleaned at least every 6 months
or when any of the needles are blocked.
Use the following procedure to remove and clean the manifold.
Lift the manifold arm and carefully pull the tubing off the connectors on the rear of
the manifold.
Dispensing Tube
Locating Pin
Aspirating Tubes
Manifold Type
Locating Pin
Indicator
Figure 10-1
The manifold is removed from the instrument by removing the two screws that
hold the manifold onto the holder.
Screws
Figure 10-2
When the two screws have been removed, carefully pull the manifold away from
the holder.
10. Maintenance
Manifold Cleaning
The most important cleaning procedure for this instrument is to rinse the wash
system before the instrument is left to stand or switched off at the end of each
day.
Caution
If the manifold is not rinsed, the needles will become blocked and it will
either need expensive repairs or may be completely ruined.
The manifold is cleaned by the following procedure.

Carefully push the cleaning needles into the aspirating and dispensing needles.
The small cleaning needle is for the dispensing needles and the large cleaning
needle is for the aspirating needles.
Rinse the manifold block with distilled water to ensure that all particles have been
removed.
The manifold may also be cleaned using a gentle ultrasonic bath for 15 minutes.
When the manifold block is clean carefully reconnect the tubing and replace it into
the instrument.
Tighten the manifold into place using the two screws, ensure that the screws are
tight.
When the manifold has been refitted, switch the instrument on and perform the
priming procedure using distilled water.
Thoroughly Cleaning the Manifold

The manifold can be taken apart to ensure thorough cleaning.
When the manifold has been removed from the instrument, remove the screw
that holds the manifold together.
Front Block Needle Block
Screw
Metal Plate
Figure 10-3
Carefully pull the metal plate away from the manifold.
Carefully pull the needle block away from the front block.
Ensure that the seals are not damaged.
10. Maintenance
Figure 10-4
The needles and the chambers for the needles can now be thoroughly cleaned.
When the manifold has been cleaned, carefully insert the seals into the slot in the
front plate. Ensure that the seals are correctly located.
Twisted or missing seals may result in a leaking manifold.
Screw the front block, needle block and metal plate together.
Refit the manifold to the instrument.
If the manifold is replaced, the instrument must be calibrated for the new
manifold.
Perform Adj.Disp. Pump, Adj.Plate.Ref. and Adj.Prime.Position. which is
displayed in the service menu.
Select Adjust Manifold Type in the adjust menu.
10.1.2 Exchanging the Manifold

The instrument can be used with the following types of manifolds:
• οNormal (8, 12 and 16)
• Normal with aerosol aspiration Factory installation required
• Fill verification Factory installation required
• Cell wash manifold (8, 12 and 16)
The manifolds are fitted to the instrument using the following procedures:
1. Lift the manifold arm.
2. Carefully fit the manifold onto the manifold arm and ensure that the locating
pins are correctly inserted through the holes in the plate.
3. If the manifold detection option is fitted, ensure that the manifold type
indicator is correctly fitted between the manifold type detector switches.
4. Tighten the manifold into place using the two screws.
5. Fit the dispensing tube onto the top connector on the rear of the manifold.
6. Fit the aspirating tubing (marked with red rings) onto the two bottom
connectors on the rear of the manifold.
7. If the aerosol pump option is fitted, fit the aerosol tubing (marked with yellow
rings) onto the aerosol connectors of the aerosol part.
8. If the Fill Verification option is fitted, connect the small ribbon cable onto the
connector block on the left-hand side of the circuit board in the manifold arm.
10. Maintenance
Manifold for the Standard Instrument

(without Aerosol Aspiration and Fill Verification)
Dispensing Tube Connectors (blue)
Locating Pin
Locating Pin
Manifold Type
Indicator
Aspirating Tube Connectors (red)
Figure 10-5
Manifold for Instrument with Aerosol Aspiration and Fill Verification

Aspirating Tubes
Connectors (red) Dispensing Tube Ribbon Cable
Connectors (blue)
Ribbon Cable
Connectors
For a 8 or 12 1
manifold only use 2
connector 2
Figure 10-6
When all the required tubing and ribbon cable have been connected, lower the
manifold arm.
10. Maintenance
10.1.3 Exchanging the Plate Support

The instrument is delivered with the plate support already installed. If the plate
support is removed, for example: for cleaning, it can be replaced using the
following procedure:
Lift the manifold arm
Slightly tilt the plate support towards the right.
Figure 10-7
Carefully slide the plate support with the waste sink at the rear over the guide bar.
Figure 10-8
The plate support must be slightly tilted so that it goes over the pin that activates
the automatic microplate centering mechanism.
To reinstall the plate support follow the procedure listed below:
Push the plate support so that it is fully inserted into the instrument.
Lower the plate support.
Lower the manifold arm.
10. Maintenance
10.1.4 Instrument Disinfection

All parts of the instrument that come into contact with the patient sera or positive
control samples must be treated as potentially infectious areas.
It is advisable to wear gloves when performing the measurement

procedure and also when making adjustments to the instrument.
It is very important that the instrument is thoroughly disinfected before it is

removed from the laboratory or any servicing is performed on it.
Before the instrument is returned to the distributor for servicing, it must be
disinfected and a disinfection certificate completed. If a disinfection certificate is
not supplied, the instrument may not be accepted by the servicing center or it
may be held by the customs authorities.
Disinfection Procedure
Caution
Before starting the disinfection procedure use a rinse procedure (Rinse
Day) with distilled / deionized water (lab quality) to flush the system.
If the laboratory has no specific disinfection procedure, the following procedure

should be used to disinfect the instrument.
The instrument should be disinfected using one of the following solutions:
Lysetol Manufacturer: Schülke & Mayr Ges.m.b.H.
Aseptisol Manufacturer: Bode Chemie Hamburg
If neither of these solutions is available 70% ethanol should be used as an
alternative.
WARNING
THE DISINFECTION PROCEDURE SHOULD BE PERFORMED BY
AUTHORIZED TRAINED PERSONNEL IN A WELL VENTILATED ROOM
WEARING DISPOSABLE GLOVES AND PROTECTIVE GLASSES AND
CLOTHING.
Please note that the disinfectant can influence the performance of your
instrument if it applied inside the instrument.
The following procedure should be used to disinfect the instrument.
1. Wear protective gloves, protective glasses and protective clothing.
2. Prepare an autoclaveable bag for all disposables used during the
disinfection procedure and label it with autoclave tape.
3. Rinse the complete pump and tube system with the disinfectant. Fill the
system with the disinfectant solution and allow a contact time of five hours.
4. Disconnect the instrument from the mains power supply.
5. Disconnect the instrument from any accessories that are used for example:
printer, computer, cartridge and so forth. Accessories that should be shipped
together with the instrument have to be included in the disinfection
procedure.
10. Maintenance
6. Carefully spray the disinfectant solution (or use a disposable soft tissue
paper towel soaked in the disinfectant) on all outer surfaces of the
instrument.
7. After a minimum contact time of 10 minutes, repeat the previous step.
8. After a contact time of five hours wipe the instrument using a soft paper
towel and a mild detergent or distilled water to remove all traces of the
disinfectant.
9. Wipe dry the outer surfaces of the instrument.
10. Pack the instrument and its accessories.
11. Disinfect your hands and clean them with a mild detergent.
12. Complete a disinfection certificate and attach it to the outside of the
box so that it is clearly visible. See below for an example of the
disinfection certificate.
Disinfection Certificate
This disinfection certificate must be completed before the instrument is for
servicing.
The certificate must be included at the top of the package in which the instrument
is returned.
I declare that the instrument in this package has been decontaminated or

disinfected to remove or inactivate any biological material, which could be
dangerous to the service personnel, or that it has never been exposed to
any hazardous biological material.
Name:...................................................................................................................
Firm: .....................................................................................................................
Address: ...............................................................................................................
.............................................................................................................................
.............................................................................................................................
Country:................................................................................................................
Signature:.............................................................................................................
10. Maintenance
10.2 Preventive Maintenance Plan

10.2.1 Daily
• Perform Rinse Night with distilled water.
10.2.2 Weekly
• Perform Rinse Night with distilled water.
• Check the liquid filter(s) in wash bottle(s) for particles and rinse liquid filter(s)
with water.
10.2.3 Every Six Months

• Clean the plate support guide bar with 70% ethanol.
• Check the centering mechanism of the plate carrier and clean if necessary
with 70 % ethanol.
• Clean the Manifold aspirating and dispensing needles.
10.2.4 Yearly (Service Technician Required)

• Save the customers specific programs using the Winwash software.
• Disinfect the instrument. (See chapter 10.1.4)
• Replace all aspirating and dispensing tubings.
• Replace the liquid filter(s) in the wash bottle(s).
• Replace the manifold sealing and clean the inner parts of the manifold.
• Check the aspirating pump and replace if necessary.
• Recalibrate the dispensing pump. (See chapter 3)
• Download the specific saved programs to the washer.
10. Maintenance
10.3 Service Tools

10.3.1 Adjustment Plate (Reference Plate)
The Adjust Plate Reference is used to define the plate position in order that the
manifold needles are correctly positioned. The adjustment should always be
positioned as shown below for all manifolds. The procedure is the same for
manifolds; 8, 12 and 16. Place the adjustment plate on the plate support and
enter the Service menu.
alignment
points for the
8 manifold
needle
Adjustment Plate
For more information about Adjustment Plate Reference, see chapter 3.
10. Maintenance
10.4 Electrostatic Discharge Information

A sudden discharge of static electricity from your finger or other conductor can
destroy static-sensitive devices or microcircuitry. Often the spark is neither felt
nor heard, but damage occurs. An electronic device exposed to electrostatic
discharge (ESD) may not be affected at all and can work perfectly throughout a
normal cycle. Or it may function normally for a while, then degrade in the internal
layers, reducing its life expectancy.
Networks built into many integrated circuits provide some protection, but in many
cases, the discharge contains enough power to alter device parameters or melt
silicon junctions.
10.4.1 Generating Static

Table 4-1 shows how different activities generate static electricity at different
electrostatic voltage levels.
Table 4-1. Typical Electrostatic Voltages
Relative Humidity
Event 10% 40% 55%
Walking across carpet 35,000 V 15,000 V 7,500 V

Walking across vinyl floor 12,000 V 5,000 V 3,000 V
Motions of bench worker 6,000 V 800 V 400 V
Removing DIPs* from plastic tube 2,000 V 700 V 400 V
Removing DIPs* from vinyl tray 11,500 V 4,000 V 2,000 V
Removing DIPs* from Styrofoam 14,500 V 5,000 V 3,500 V
Removing bubble pack from PCB 26,500 V 20,000 V 7,000 V
Packing PCBs in foam-lined box 21,000 V 11,000 V 5,000 V
* Dual Inline Packaging (DIP) is the packaging around individual microcircuitry.

These are then multi-packaged inside plastic tubes, trays, or Styrofoam.
Note
700 volts can degrade a product.
10. Maintenance
10.4.2 Preventing Electrostatic Damage to Equipment

Many electronic components are sensitive to ESD. Circuitry design and structure
determine the degree of sensitivity. The following proper packaging and ground
precautions are necessary to prevent damage to electric components and
accessories:
• To avoid hand contact, transport products in static-safe containers such as
tubes, bags, or boxes.
• Protect all electrostatic parts and assemblies with conductive or approved
containers or packaging.
• Keep electrostatic sensitive parts in their containers until they arrive at static-
free stations.
• Place items on a grounded surface before removing them from their
container.
• Always be properly grounded when touching a sensitive component or
assembly.
• Avoid contact with pins, leads or circuitry.
• Place reusable electrostatic-sensitive parts from assemblies in protective
packaging or conductive foam.
10.4.3 Personal Grounding Methods

The method for grounding must include either a wrist strap or a foot strap at a
grounded workstation. When seated, wear a wrist strap connected to a grounded
system. When standing, use footstraps and a grounded floor mat. Wrist Straps
are flexible straps with a minimum of one-megohm +/- 10% resistance in the
ground cords. To provide proper ground, a strap must be worn snug against bare
skin. The ground cord must be connected and fit snugly into the banana plug
connector on the grounding mat or workstation.
Therefore Tecan recommends that you use a grounded wrist strap

whenever you service or clean an instrument. For all components marked
with ESD – Sticker it is absolutely necessary to use the grounded wrist
strap to avoid damaging any electrical parts!

11.1 Introduction
Error Description Possible Causes See Chapter
Program not defined No test defined in the instrument memory 3
Program too Big If more than 60 process steps have been defined 3
No END in Prog. If the test definition (with END step) is not completed. 3
Wrong Manifold Manifold not correctly defined in Adjust Menu 3
Transport Error Instrument cannot move plate support 6
Î INIT Error Check if the plate support is correctly inserted and the plate
support is not blocked. Ensure the plate support motor is
not defective or blocked and the home position optical
switch is not broken.
Incorrect Plate Position Plate parameters are not defined 3
(Adjust Menu)
Disp. Sens. Err. Dispensing pump, optical switch, disp. pump/ main board 5&7
cable or main board broken
No figures in display but Display board broken 7
display light on
Display and LED dark Power supply board broken 7
Defective fuse
No or incorrect Dispensing needles blocked 5
dispensing Dispensing valve broken
Dispensing pump broken
No or incorrect Aspirating pump broken 5
aspirating Aspirating needles blocked
Waste Bottle Full at The instrument checks if the waste bottle is full at the 3
Start beginning of a selected program
Waste Bottle Full during The waste bottle is full during the washing procedure 3
PROCEDURE
Liquid Bottle Empty Before the program is started, the instrument can detect if 3
the liquid bottle is empty
Printer Error Defective printer or printer cable 3
Init.Error: BCR Change barcode chips U21 and U22 9
Manifold Arm Error The manifold arm does not move up and down 3
Fill Comm. Err No communication between main board and fill verification 3
board
Inadequate wash Instrument detects that some of the needles are not 3
dispensing solution
Check Disp. Path The instrument detects if any of the needles are dispensing 3
no or too little solution
Fill Funct. Err. If the fill verification board needle status can not be deleted 3
Fill Asp. Err When the well is not correctly aspirated 3
New Firmware Empty Battery 7
Auto Clear All

12.1 Introduction
The table below lists the physical specifications for the instrument. All
specifications listed below are based on the following instrument settings:
Dispensing speed: from 1 – 5 (standard setting is 3)
Aspirating rate: from 1 – 5 (standard setting is 3)
PARAMETERS CHARACTERISTICS
General
Display unit Liquid Crystal Display with two rows of sixteen digits
Keyboard Four key membrane keyboard
Number of dispensing Up to four channels
channels
Manifold types 8, 12 or 16 way or 384 Well Manifold
Variable
Volume of solution 50 - 3000 µl in 50 µl steps for washing
dispensed (50 - 400 µl if 'Dispense rate: drip' is selected)
50 - 400 µl in 50 µl steps for dispensing
For the 384 Well Manifold:
10 - 120 µl in 10 µl steps for dispensing.
Dispensing accuracy CV: <= 4% over the plate
(300µl dispensing volume, dispensing rate: 3).
This is also for the 384 Well Manifold.
Residual volume Crosswise aspiration: <=2 µl per well
single aspiration - cell wash manifold: <= 5 µl per well
(Asp. rate 5, Asp. time 4 sec., Asp. speed 10mm/sec)
For the 384 Well Manifold:
Crosswise aspiration: <=2 µl per well
(Asp. rate 5, Asp. time 4 sec., Asp. speed 10mm/sec)
Interface All connected devices must be approved and listed as per
EN 60950, UL 1950 or CSA C22.2 No. 950 for Data
Processing Devices.
Serial RS 232 C 9600 Baud (fixed)
Power
Supply Auto sensing for the settings
100 - 120 volt or 220 - 240 volt, 50 / 60 Hz
Consumption < 65 VA
Main fuse 2 x 1.6 T Amp. (slow blow)
Physical
Outside dimensions Width : 28,5 cm, Depth : 39,5 cm, Height:: 22,0 cm
Weight 8.2 kg
Environmental
Ambient temperature
Operation 15°C to 35°C (59°F to 95°F)
Non-operation Below: -10°C (14°F) Above: 43°C (109°F)
Storage -40°C to 70°C (-40°F to 158°F)
Relative Humidity
Operation 20 % to 90 % no condensing
Non-operation Below: 5 % Above: 95 %
Storage 5 % to 95 %
Others
Overvoltage category II
Pollution degree 2
Method of disposal Contaminated waste
Linear Shaking
Mode Shaking Speed
1 2 3
Low 1.2 mm 4 Hz 7.5 Hz 11.0 Hz
Normal 1.7 mm 2 Hz 5.0 Hz 8.0 Hz
High 2.6 mm 2 Hz 3.5 Hz 5.0 Hz
The instruments conform to the legal norms at date. For more information, see
the Declaration of Conformity Certificate on the last page of this manual.
Index
Index
A L
Adjust Menu Liquid Detection............................................ 3-49
Exit ............................................................ 3-23 Liquid Sensors....................................... 2-5, 3-22
Adjust Times................................................. 3-84 M
Aerosol Pump ............................................... 3-42 Manifold
B Cleaning.................................................... 10-2
Barcode Reader ....................................2-6, 3-48 Manifold Motor Testing................................. 3-40
C Motor Testing ............................................... 3-38
Cleaning........................................................ 10-1 O
D Options ......................................................... 3-51
Detector Unit Cable ........................................ 2-5 P
Dispensing Pump ......................................... 3-42 Plate Motor Testing ...................................... 3-39
Adjust ........................................................ 3-55 Programming................................................ 3-28
Display Unit .................................................... 7-8 Pump Testing ............................................... 3-41
E R
Error Messages ............................................ 3-95 Round Bottom Plates ................................... 3-10
F S
Fill Verification Sensor Function
Testing ...................................................... 3-47 Test........................................................... 3-46
Flat Bottom Plates ........................................ 3-11 Service Menu ............................................... 3-26
Flat Bottom Wells ......................................... 3-12 Show Detected CPU .................................... 3-49
I Solenoid
Installing the Instrument ................................. 2-1 Testing ...................................................... 3-43
Instrument Support Screws ............................................ 6-5
Connections ................................................ 1-2 W
Description .................................................. 1-2 Waste Sensor............................................... 3-22
K
Keyboard .................................................1-6, 7-8
2006-02 Technical Manual for Columbus Washer No. 30000294 Rev. No. 1.7 Index

Columbus Washer

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3. Firmware and Software Description

4. Removing and Replacing the Instruments Cover

11. Trouble Shooting

12. Instrument Specifications

The instrument should only be opened by authorized personnel. Before the

Ensure that the instrument has been disinfected before it is serviced.

1.2 Instrument Description

1.2.1 Rear Panel Connections

ON/OFF Inlets for channels 1 - 4

Hand held Bottle Holder with Printer Connector Aerosol Waste

(Instrument with Pinch Valves)

Channel 1 Channel 2 Channel 3 Channel 4

Hand held Bottle Holder with Printer Connector Aerosol Waste

All connected devices must be proved and listed with regulations

1.2.2 Connection Diagram for Columbus (1 Liquid Channel)*

* Some of the instruments may include the prime valve.

1.2.3 Connection Diagram for Columbus (4 Liquid Channels)*

Hand held Barcode

None Return Aerosol

grey red yellow purple

Bottle Holders with liquid

* Some of the instruments may include the prime valve.

1.2.4 Keyboard and Display Unit

Key 1 Key 2 Key 3 Key 4

1.3 Interconnection Diagram

Power Supply Board

* The Orbital Shaking option is not used.

Liquid Sensor System Accessory package

2.2 Unpacking Procedure

2.3 Power Requirements

2.4 Installation Procedure

2.4.1 Installing the Instrument

2.4.2 Connecting the Waste Tubing

Aerosol Tube Hole Waste Tube Hole

Solution Tubes to Manifold

Turn the instrument back to the normal position.

Aerosol Tube to Manifold

Solution Tubes to Manifold

Turn the instrument back to the normal position.

2.4.3 Connecting the Solution Tubes

Inlets for channels 1 - 4

Aerosol Tube* Waste Tube

2.4.4 Fitting and Connecting the Liquid Sensors (Optional)

Fix the tube support in the bottle holder as shown below.

2.4.5 Connecting the Barcode Reader (Optional)

Hand Held Barcode Liquid Sensor

2.4.6 Connecting the Printer (Optional)

2.4.7 Power Connections

Before the instrument is installed and switched on, it should be left to

3. Firmware and Software

3.2 Onboard Software Menus

The priming procedure is performed using the following procedure:

Prime Ch1 Select required channel to be primed

Prime Solution ? Required solution in bottle and inlet

Waste Bottle OK? Waste bottle empty

Prime ChX Y sec Priming procedure being performed