SOP ON LIS ON ECL 412

The ECL 412 can be connected to an LIS and send automatically the results to the host
computer.
The instrument can be connected 2 different ways:
1. By serial port (RS232)
2. By Ethernet (RJ45)

Depending on the type of connexion the system needs to be configured accordingly.

Once the LIS window opens by default with Serial connexion option selected
It displays the information needed to be configured to achieve the communication with the
Host computer. This includes: Port #, speed, data bit, parity, stop bits, flow control, etc.

If the Ethernet type connexion is selected then the host IP needs to be entered in the
appropriate field. Click on the Settings button to access the Device Setting

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 1.1.1. Service
Service is only accessible to your technical service representative and is protected by
special access codes.

1.2. General

The ECL412/105 System is linkable to a LIS (Laboratory Information System).

For that you will need to :

- Physically connect the ECL412/105 System to the laboratory system

- Configure the ECL412/105 System

Connection may be done either by serial port or network connection.

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 1.3. Hardware configuration
The connection uses a RS 232 serial interface with DB-9 connector for Serial
communication or Ethernet interface RJ45 connector between the ECL412/105 System and
the Host computer.

1.3.1. Serial communication

The connection uses a RS 232 serial interface with DB-9 connector

1.3.1.1. Cable specifications

RS232 Pin Assignments (DB9 PC signal set) Data :

Received Line Signal Detector Low level :
Pin 1 +5  +20 V
(Data Carrier Detect) (DCD)
High level :
Pin 2 Received Data (RD)
-5  -20 V
Pin 3 Transmit Data (TD)
Pin 4 Data Terminal Ready (DTR) Control :
Pin 5 Signal Ground OFF :
+5  +20 V
Pin 6 Data Set Ready (DSR) ON :
Pin 7 Request To Send (RTS) -5  -20 V
Pin 8 Clear To Send (CTS)
Pin 9 Ring Indicator

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 1.3.1.2. ECL412/105 RS232 connection

The serial cable must be plugged to the standard DB9 connectors of the PC, referenced in
the Windows system as « COM Ports ».

If it is not the case, you must plug in the PC either an electronic card (RS 232 serial card),
or use an USB-Serial adapter (if you PC has USB ports).

1.3.2. Network communication

The network connection is done by plugging an Ethernet RJ 45 Category 5 cable connector

to the network connector of ECL412/105.

To configure the network, contact ERBA MANNHEIM support.

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 1.4. Work mode

The ECL412/105 System only sends the result data of measurement. When a measurement
is done the system automatically tries to send on the selected LIS port.

1.5. Protocols

When using serial port, the ECL412/105 System works with 2 standard protocols:
- ASTM 1381 for « physical » communication : this protocol describes the
mechanisms of data send
- ASTM E 1394 for « logical » communication : this protocol describes the
mechanism of data coding ( test requests, queries, results)

With Network communication, only ASTM E 1394 protocol is used: the physical protocol
is the chosen network protocol (generally TCP/IP).

1.5.1. Physical protocol: ASTM 1381

The frame format is :

<STX><Frame #><Data><ETX><Checksum><CR><LF>
(for more information about this protocol , visit www.astm.org )

The function schematics is resumed in the next page.

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 START

Wait receiving ENQ

ENQ Received
5 seconds

EOT received
Send ACK File reception complete

N
New files in
\ASTM\OUT Wait Frame reception
folder
?
Frame reception
Y

Frame analysis

Y N
Frame OK ? Send NAK

Connection Established

Send ENQ
NAK ACK
10 Received Received
seconds
Other file to send ?
n > nb frames of the file
Send Frame N
Send EOT - file completed =>
ACK NAK TimeOut 5s Move in \ASTM\OUT\Done
Received received folder
n=n+1
Completed

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 1.5.2. Logical protocol : ASTM E 1394
The Logical protocol ASTM E 1394 allows communication between LIS and ECL412/105
System : Send the results

(for more information about this protocol , visit www.astm.org )

1.5.3. Results

Once tests are completed, the ECL412/105 System sends the results.
A result message contains only data for one sample but it may contain one or more result
for one or more analysis.

A result message is composed of:

a H line (header)
a P line (patient)
one O line or more (order)
for every O line , one R line (result)or more
a L line (end of message)

Example :

H|\^&|||||||ECL_412
P|1||1
O|1|1||^^^Fibriogen|||||
R|1|^^^Fibriogen|34.884365|Sec||||||||20150625162726||
L|1

Sending several results :

When the transmission of several results of an analysis is needed (for example , PT with
seconds, percent and INR):

For a PT Test transmission will be:

3 results lines are sent with the same code (PT)

H|\^&|||||||ECL_412
P|1||1
O|1|1||^^^PT|||||
R|1|^^^PT|34.884365|%||||||||20150625162726||
R|2|^^^PT|0.000000|INR||||||||20150625162726||
R|3|^^^PT|9.200000|Sec||||||||20150625162726||
L|1

Date/Time of execution :

The 13th Field contains date and time when test has been completed on the ECL412/105
This date format is YYYYMMDDHHMMSS

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Fields Table:

Field Contenu Remarque/Valeur

H Line
Field n°1 :
1st character H
2nd character Field delimiter generally : |
3rd character Repetition delimiter generally : \
4th character Component delimiter generally : ^
5th character Escape character generally : & but unused
2==>7 Unused fields
8 Automate ID ECL_412 or ECL_105
Ligne P
1P
2 Sequence number
3 Unused field
4 Patient ID
Ligne O
1O
2 Sequence number
3 Sample ID
4 Unused fields
5 Analysis parameters :
component # 1,2,3 : unused
component # 4 : Analysis parameters

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