Professional Documents
Culture Documents
1 - Gause 1934, Experimental Analysis of Vito Volterra - S Mathematical Theroy of The Struggle For Existence
1 - Gause 1934, Experimental Analysis of Vito Volterra - S Mathematical Theroy of The Struggle For Existence
2036
tinuously until all the plants are dead. The length chronic ailments, has demonstrated the practical value
of time each plant survives is used as a criterion of of a simple medium such as Sabouraud’s. By means
drought resistance, together with the moisture content of a modified formula for this medium one strain of
of the soil at death. Ordinarily only a few plants Actinomycetes was found to be acid-fast, although it
remain alive until they have exhausted the soil mois- was non-acid-fast on such media as standard Bordet-
ture to critical values. Gengou, potato, synthetic phosphate and Difco Sa-
A description of a sample run follows. The plants bouraud.
tested were white spruce, Picea canadensis, of three The formula which we employ consists of 4 per
different classes, viz.: 2-0 (two-year old seedlings), cent. maltose, 1 per cent. Difeo peptone, 1.8 per cent.
3-0 (three-year old seedlings) and 2~1 (three-year old flaked agar dissolved in unfiltered beef heart or veal
trees which have had two years in the seed-bed and infusion instead of water. No adjustment in reaction
one year in the transplant bed). Ten plants of each is made. Glycerine and other carbohydrates may bi:
class were used. The temperatures averaged 38 de- added if desired. Slanted agar favors development
grees C. and the relative humidity about 10 per cent. of acid fastness in about four days. A grayish brown
The soil moisture at death was above 14 per cent. for powdery substance develops upon the upper portion
papro[UMOdg
all pots. The results are shown in Table 1. of the slant simultaneously with the appearance of the
acid-fast portions of growth.
TABLE 1
The strain was isolated from the blood of a case of
acute mastoiditis complicated by sinus thrombosis,
Class of stock Number of days surviving septicemia and arthritis. The acid-fast component
WO}
Mean Standard error
appeared in young cultures (seventy-two hours) on
1e S10'aouatos MMay/:sdny
2-0 20.2 1.2 this medium as branching non-acid-fast mycelia con-
8-0 oe. 21.4 15 taining acid-fast pleomorphic portions. Old cultures
2-1 13.2 9 consisted of non-acid-fast oval components and myee-
lia interspersed with acid-fast oval-shaped compo-
In this case the transplant stock proved to be far nents. The acid-fast characteristic was inhibited on
less resistant to atmospheric drought than either of all other media. The other strains of Actinomycetes
the two classes of seedlings. were consistently non-acid-fast on all media employed
so far.
O&8S 3p SpEpIsiaaluy]
Harpy L. SHIRLEY
Lake Srares Forest EXPERIMENT Sabouraud’s medium, in which unfiltered meat in-
Srarion fusion is employed in place of water, is therefore
recommended for cultivation of Actinomycetes iso-
A MODIFIED SABOURAUD MEDIUM SUIT- lated from human tissue. An attempt is being made
ABLE FOR CULTIVATION OF ACID- to standardize this type of unadjusted unfiltered
FAST ACTINOMYCETES medium.
O[Ne_
TuHE study of five strains of Actinomycetes isolated Sara A, Scupper
at this hospital during the past four years from the Dept. or PatHowocy, Ciry Hosprran
uo
blood of patients suffering both from acute and New York, N, Y.
Jaquadag
SPECIAL ARTICLES
ZO
EXPERIMENTAL ANALYSIS OF VITO VOL- Since, however, this book will appear only after some
TCOT
papro[UMod
limited amount of energy between two populations. ‘T ne | P condstum
In order to investigate the competition between 3 8 ‘0 t Lg cn aniend cobtore
s oy 8-0 se Zs
species for a source of energy kept at a certain level
experiments were made with various protozoa, and
%
Wo}
very clear and convincing results were obtained for
Paramecium caudatum and Paramecium aurelia. 5
1e S10-aouatos MMay/:sdny
These infusoria were cultivated in a buffered balanced >
Osterhout’s salt solution (pH =8.0), in which a sus-
pension was made of Bacillus pyocyaneus (of fixed
density). It has been found that bacteria do not mul-
tiply under these conditions.2, Every day infusoria
were centrifuged, the nutritive medium changed, and s ”
every other day the microcosms underwent a cleansing Days
process with a salt solution. Specially arranged ex- Fie, 1
O&8S 3p SpEpIsiaaluy]
periments showed that the deficiency of food was the microcosm. Therefore, it is not the interaction itself,
only limiting factor in these cases. We had under as would be expected from the mathematical theory
such conditions (a) a competition of P. caudatum developed by Lotka (1920) and by Volterra (1926),
with P. aurelia for the still unutilized food resources, but the constant interference from without that leads
but after the source of energy had been altogether to the oscillation in numbers. The corresponding
taken hold of, we had (b) a redistribution of energy
O[Ne_
equation of the struggle for existence has no periodic
between two components which always resulted in a solution. This is owing to the particular biological
uo
complete driving out of P. caudatum by P. aurelia adaptations of our predators, which have not been
Jaquadag
(Fig. 1). All this agrees with the mathematical foreseen in the theoretical equations. In our experi-
theory. The corresponding equations are somewhat ments an analysis was made of the role of cover or
complicated, beeause the coefficients of the struggle refuge for the prey in the processes of the struggle
for existence vary with time: one species may be fa-
ZO
the experiment, and the depression of one species by microcosm complicated, instead of the “deterministic”
another will only begin later on. processes subject to differential equations we are eon-
The destruction of one species by another has been fronted with “probabilities of change” in one direc-
studied with Paramecium caudatum being devoured tion or another. The corresponding material will be
by another infusoria, Didinium nasutum. Experi- found in the above-mentioned book.
ments showed that this biological system presents no G. F. Gauss
oscillations in the numbers of individuals peculiar to ZooLoaicaL INSTITUTE
itself, and that in spite of abundant food for Para- UNIVERSITY oF Moscow
mecium the latter are completely destroyed by preda-
THE AMYLASE SYSTEM OF THE LIVER
tors which ‘perish in their turn later on. However,
Comparison of rat liver preparations, using the
oscillations appear if we admit a controlled and simul-
taneous immigration of predators and prey into the
method for observing starch digestion introduced by
Waldschmidt-Leitz and Samec,! showed that the
1Gause, Jour. Exp. Biol., 9, p. 389.
2 Johnson, Physiol. Zool., 6, p. 22. 1 Zeitschr. fiir physiol. Chem., 203: 16, 1931,
Science
Science (ISSN 1095-9203) is published by the American Association for the Advancement of Science. 1200 New
York Avenue NW, Washington, DC 20005. The title Science is a registered trademark of AAAS.