Hemaray 86 Service Manual V1.0e

Hemaray 86/89 Service Manual
Copyright: Rayto Life and Analytical Sciences Co., Ltd.

Address: C&D/4F, 7th Xinghua Industrial Bldg, Nanhai Rd, Nanshan,
Shenzhen 518067, P.R. China
Hemaray 86 / 89 Service Manual
Rev: 1.0e
1
Main Alarm Icons Used in this Instrument
Caution
Biological and chemical hazards
Laser warning
High voltage warning
The operator should operate according to the

instruction under the icon, otherwise personal
injury may be caused.
Puncture warning
2
Table of Contents
MAIN ALARM ICONS USED IN THIS INSTRUMENT.........................1
TABLE OF CONTENTS.......................................................................2
CHAPTER 1 INTRODUCTION..........................................................6
1.1 Introduction....................................................................................................6
1.1.1 Product Name......................................................................................................................6
1.1.2 Model.....................................................................................................................................6
1.1.3 Features................................................................................................................................6
1.1.4 Test Parameters...................................................................................................................6
1.2 Composition and Structure............................................................................7
1.3 Working Principle...........................................................................................9
1.3.1 Overview...............................................................................................................................9
1.3.2 Sample Suction....................................................................................................................9
1.3.3 White Blood Cell (WBC) Testing Principle....................................................................10
1.3.4 Hemoglobin Concentration (HGB) Measurement........................................................12
1.3.5 Red Blood Cell (RBC)/Platelet (PLT) Measurement...................................................12
1.3.6 Parameters.........................................................................................................................13
1.4 Performance................................................................................................14
1.5 Scope of Application....................................................................................16
1.6 Technical Parameters..................................................................................16
1.7 PC Configuration.........................................................................................16
CHAPTER 2 INSTALLATION...........................................................18
2.1 Unpacking....................................................................................................18
2.1.1 Steps of Unpacking...........................................................................................................18
2.1.2 Handling Method...............................................................................................................18
2.2 Installation and Use Environment................................................................18
2.3 Requirements of Power Supply...................................................................19
2.4 Requirements of Space...............................................................................19
2.5 Removal of Auxiliary Fixing Structure for Transportation............................19
2.6 Connection of Reagent and Waste Liquid Containers................................19
2.7 Connecting Power Supply...........................................................................20
2.8 Preparation before Power-on......................................................................20
2.9 Debugging....................................................................................................23
2.10 Software Installation and Upgrade...........................................................27
2.10.1 Installation of XP OS.........................................................................................................27
2.10.2 Installation of Information Processing System Software............................................28
2.10.3 Installation of Main Control Board System Program...................................................29
2.10.4 Installation of Main board application Software...........................................................32
2.10.5 Installation of Driver Board Program..............................................................................32
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2.10.6 Installation of Automatic Sampler Driver Board Program...........................................34

2.10.7 Installation of Fluidics Sequence....................................................................................34
CHAPTER 3 HARDWARE SYSTEM...............................................36

3.1 Position of Electrical Circuit Unit..................................................................36
3.2 Power Assy Section.....................................................................................38
3.3 Shell Assy Section.......................................................................................39
3.4 Main Control Board......................................................................................39
3.4.1 Basic Functions and Module Division............................................................................39
3.4.2 Principle..............................................................................................................................40
3.4.3 PCB Layout Diagram........................................................................................................40
3.4.4 Connector Pin Assignment..............................................................................................41
3.5 Optical System Related Boards...................................................................47
3.5.1 Forward Small-angle Preamplification Board...............................................................47
3.5.2 Forward Large-angle Preamplification Board...............................................................48
3.5.3 Side Scatter Preamplification Board..............................................................................49
3.5.4 Laser Driver Board............................................................................................................50
3.6 Power Driver Board.....................................................................................53
3.6.1 Basic Functions.................................................................................................................53
3.6.2 Functional Block Diagram................................................................................................53
3.6.3 Connector Assignment.....................................................................................................55
3.6.4 Main Test Points................................................................................................................60
3.7 High Voltage Switchboard............................................................................61
3.7.2 Functional Block Diagram of High Voltage Switchboard............................................62
3.7.3 PCB Layout Diagram of High Voltage Switchboard....................................................62
3.7.4 Connectors on High Voltage Switchboard....................................................................63
3.7.5 Main Test Points of High Voltage Switchboard............................................................65
3.8 Autoloader board.........................................................................................65
3.8.2 Principle..............................................................................................................................65
3.8.3 PCB Layout Diagram........................................................................................................65
3.8.4 Connector Pin Assignment..............................................................................................66
3.9 Other Boards................................................................................................70
3.9.1 LED Display........................................................................................................................70
3.9.2 Network Port Board...........................................................................................................72
3.9.3 Key Board (for automated model)..................................................................................73
3.9.4 Reflective Optocoupler Adapter Board (for automated model).................................74
3.10 Wire Interconnection Diagram..................................................................75
CHAPTER 4 SOFTWARE SYSTEM................................................80

4.1 Overall Software Architecture......................................................................80
4.2 Functions of Information Processing System Software...............................81
4
CHAPTER 5 FLUIDICS SYSTEM....................................................83

5.1 Functions of Fluidics Devices......................................................................83
5.1.1 Valves..................................................................................................................................83
5.1.2 Syringes..............................................................................................................................83
5.1.3 Other Auxiliary Devices....................................................................................................84
5.2 Whole Machine Test Process......................................................................84
5.2.1 Flowchart of CBC+DIFF Test in Whole Blood Mode...................................................84
5.2.2 Process of CBC Test in Whole Blood Mode.................................................................85
5.2.3 Flow chart of CBC+DIFF Test in Pre-diluted Mode.....................................................85
5.2.4 Process of CBC Test in Pre-diluted Mode.....................................................................86
5.3 CBC+DIFF Measurement Sequence Description.......................................86
5.3.1 DIFF Bath Sample Distribution and Blending after 86D Lyse Adding (0~14S)......86
5.3.2 DIFF Channel Test (19~40S)..........................................................................................89
5.3.3 DIFF Channel Cleaning (40～53S)................................................................................93
5.3.4 HGB Background Measurement, WBC Bath and RBC Bath Drain, and Diluent
Spitting (0.5~15S)..............................................................................................................................96
5.3.5 WBC Bath Sample Distribution and Initial Mixture Blending (15~17.6S)................98
5.3.6 Initial Diluted Mixture Suction (19.6~21.6S).................................................................99
5.3.7 Secondary Blending after 86H Lyse Spitting to WBC (21~25S)...............................99
5.3.8 RBC Bath Sample Distribution and Blending (25~27S)...........................................100
5.3.9 WBC and RBC Test, and HGB Sample Measurement (27~46S)...........................101
5.3.10 WBC and RBC Baths Cleaning (46~60S)...................................................................103
5.4 Functions of Fluidics Sequence.................................................................104
5.4.1 Test Sequence.................................................................................................................104
5.4.2 Replacement of Reagents.............................................................................................104
5.4.3 Clean.................................................................................................................................104
5.4.4 Upkeep..............................................................................................................................104
5.4.5 Fluidics Maintenance......................................................................................................105
5.4.6 Functions of Other Sequences.....................................................................................106
CHAPTER 6 MECHANICAL SYSTEM...........................................108

6.1 Structure of Instrument..............................................................................108
6.1.1 Structure of Major Boards of Instrument.....................................................................108
6.2 Dismantling of Instrument..........................................................................112
6.2.1 Opening Left and Right Doors.......................................................................................112
6.2.2 Opening and Closing Top Shell.....................................................................................113
6.2.3 Dismantling of Shell of Automatic Sampler.................................................................115
6.2.4 Dismantling of Automatic Sampling Driver Board......................................................116
6.2.5 Dismantling of Bottom Shell..........................................................................................118
6.2.6 Dismantling of Top Plate................................................................................................120
6.2.7 Dismantling of Rear Door...............................................................................................121
6.2.8 Dismantling of Cooling Fan, Main Control Board, and Driver Board......................122
6.2.9 Dismantling of Burning Board.......................................................................................122
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6.2.10 Dismantling of Sampling Assy Connector Board and Sampling Assy Adapter
Board 123
6.2.11 Replacement of Fuse.....................................................................................................124
6.2.12 Dismantling of Power Supply Assy...............................................................................125
6.2.13 Dismantling of DIFF Bath Assy and Counting Chamber Assy.................................126
6.2.14 Dismantling of Sampling Assy.......................................................................................127
6.2.15 Dismantling of Waste Liquid Pump Assy.....................................................................128
6.2.16 Dismantling of Syringe Assys........................................................................................129
6.2.17 Dismantling of Valves.....................................................................................................131
6.2.18 Dismantling of Liquid Pressure Sensor.......................................................................133
6.2.19 Dismantling of Optical Path Assy..................................................................................133
6.2.20 Dismantling of Board in Optical Assy...........................................................................134
6.2.21 Replacement of Flow Cell..............................................................................................137
CHAPTER 7 COMPONENT ADJUSTMENT AND DETECTING....144

7.1 Component Adjustment and Parameter Setup..........................................144
7.1.1 Sensor AD Setup.............................................................................................................144
7.1.2 Debugging of Sampling Probe Position......................................................................144
7.1.3 Waste Syringe Pressure Calibration............................................................................145
7.1.4 DIFF Bath Temperature Calibration.............................................................................146
7.1.5 CBC Gain Debugging.....................................................................................................146
7.1.6 DIFF Gain Debugging.....................................................................................................147
7.2 Test............................................................................................................149
7.2.1 Functional Test.................................................................................................................149
7.2.2 Performance Test............................................................................................................151
CHAPTER 8 MAINTENANCE........................................................153
8.1 Regular Maintenance.................................................................................153
CHAPTER 9 CLINICAL ALARM.....................................................154

9.1 Alarm Flags................................................................................................154
9.1.1 There is no parameter result.........................................................................................154
9.1.2 Parameter result with alarm flag...................................................................................154
9.1.3 Alarm Messages..............................................................................................................155
9.2 Histogram...................................................................................................156
CHAPTER 10 FAULTS AND TREATMENT...................................157

10.1 Overview of Fault Messages..................................................................157
10.2 Whole Machine Treatment......................................................................158
10.3 Alarm Treatment for Automatic Sampler Section...................................163
10.4 Treatment of Other Common Faults of Analyzer....................................165
APPENDIX I: SCHEMATIC DIAGRAM OF FLUIDICS....................170
6
7
Chapter 1 Introduction
.1 Introduction
.1.1 Product Name
Automated Hematology Analyzer
.1.2 Model
Hemaray 86, Hemaray 89
.1.3 Features
This instrument has two sampling modes, i.e. Automatic and Enclosed. The Automatic
sampling mode provides the whole blood test mode.
Hemaray 86: The automatic sampler carries up to 5 tube racks, each of which holds up to
10 samples, total 50 samples.
Hemaray 89: The automatic sampler carries up to 10 tube racks, each of which holds up to
10 samples, total 100 samples.
The Enclosed sampling mode provides the Whole Blood and Pre-diluted test modes. After
sampling, the instrument conducts the test automatically and provides WBC 5-part differentiation
results and scattergram, and parameter results and histograms of RBC and PLT.
.1.4 Test Mode CBC CBC+DIFF Parameters
The instrument WBC WBC has two test modes, i.e.
RBC RBC
CBC and CBC+DIFF. The CBC mode
HGB HGB
provides 14 blood HCT HCT parameters and 3
histograms. The MCV MCV CBC+DIFF mode
provides 28 blood MCH MCH parameters (in which, 4
MCHC MCHC
are study RDW-SD RDW-SD parameters), 2
scattergrams, and 2 RDW-CV RDW-CV histograms.
PLT PLT
Table 1-1 Test MPV MPV Parameter Table
PCT PCT
Parameter
PDW PDW
P-LCR P-LCR
- NEUT%
Caution: (1) “-” - LYMP% indicates that the
current test - MONO% mode does not
- EO%
provide it.
- BASO%
(2) Study - NEUT# parameters are
used - LYMP# for study only and
can’t - MONO# be used as basis
- EO#
for - BASO#
clinical diagnosis.
IG%
Study IG#
Parameter ALY%
.2 Composition and
ALY#
Structure WBC
-
histogram
The instrument RBC RBC mainly includes shell
assy, frame assy, histogram histogram optical system, power
PLT PLT
Chart
histogram histogram
8
Main
-
scattergram
Side
-
scattergram
supply, sampling assy, pump,valve, bath assy, etc. Its structure is shown in the figure below.
Figure 1- Front View of Instrument
1- Shell 2 - Sample compartment Door 3 - Automatic Sampler 4 - Tube Rack 5 - Liquid

Suction Key 6 - Open Door Key 7 - Status Indicator Board (Power Supply, Running, Alarm,
Laser)
Figure 1- Back View of Instrument
1 - Network Port 2 - Earthing Rod 3 - Waste Liquid Sensor 4 - Waste Liquid Pipe Connector 5
- Cleanser 6 - Diluent 7 - 86H Lyse 8 - 86D Lyse 9 - Power Supply Interface (including 2
fuses)
9
Figure 1- Axial Side View 1 of Instrument

1 - Power Supply Switch 2 - Power Supply 3 - Liquid Detection Sensor 4 - Liquid Valve 5 -
Syringe 6 – Sample compartment 7 – Liquid Pressure Sensor
Figure 1- Axial Side View 2 of Instrument

1 - Sampling Assy 2 - DIFF Bath 3 - RBC/HGB Bath 4 – Pump Assy 5 - Circuit Board 6 -
Cooling Fan
.3 Working Principle
.3.1 Overview
This analyzer adopts the Coulter Principle to test the quantity and volume distribution of
WBC, RBC and PLT, adopts the colorimetry to measure hemoglobin concentration, and adopts
the semiconductor laser and flow cell analysis technique to obtain WBC 5-part differentiation.
Based on this, the analyzer calculates the results of other parameters.
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.3.2 Sample Suction

In the Whole Blood work mode, the analyzer sucks 20μL (CBC+DIFF mode) or 10μL (CBC
mode) whole blood sample.
In the Pre-diluted work mode, you should first blend the 20μL peripheral blood sample and
180μL diluent outside the machine to form a 1:10 diluted sample, and carry the diluted sample
to the analyzer for sampling. Here, the analyzer sucks 80μL (CBC+DIFF mode) or 40μL (CBC
mode) diluted sample.
.3.3 White Blood Cell (WBC) Testing Principle
The basic principle for WBC testing is the electrical impedance method (Coulter Principle)
and laser scattering method.
The testing principle for the electrical impedance method (Coulter Principle) is shown in the
figure below.
During the test with the electrical impedance method, the sample being tested, after diluted,
enters the WBC test unit which has a small opening called “test hole(aperture)”. The hole has a
pair of positive and negative electrodes on both sides that are connected to the constant current
power supply. As blood cells have a feature of bad conductor, when the blood cells in the diluted
sample pass through the test hole under the constant negative pressure, the resistance between
electrodes will change, thus forms a pulse signal with the size in proportion to the volume of the
cell on both ends of the electrode. When the cells pass through the hole continuously, a series
of electrical pulses will be produced on both ends of the electrode. The quantity of these
electrical pulses reflects the number of blood cells.
Compare the amplified electrical pulses collected with the channel voltage threshold
corresponding to the normal WBC range, and calculate the number of electrical pulses in the
WBC channel of the electrical pulse amplitude. Thus, all electrical pulses collected are classified
according to the different channel voltage thresholds, and the number of electrical pulses in the
WBC channel is the number of WBCs. The number of cells in each channel range divided based
on the pulse voltage amplitude decides the volume distribution of cells. The two-dimensional
diagram with the x-coordinate representing cell volume and the y-coordinate representing
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relative cell quantity is the histogram reflecting the cell population distribution.
In the laser scattering method, when a certain amount of diluted blood sample is injected
into the Flow Cell, wrapped by the sheath fluid formed by the diluent, the cells lining up one by
one pass through the center of the Flow Cell. When the blood cells suspending in the sheath
fluid pass through the laser detection area, the blood cells are irradiated by the laser beam, and
the nature of the scattered light produced is relating to the cell size, cytomembrane, and
refractive index of the internal structure of cell. The schematic diagram of laser scattering
method is as follows:
The small-angle, large-angle and side-angle scattered lights in the direction of laser beam
are measured, which reflect the size of blood cell, complexity of cell nucleus, and granularity
respectively. The DIFF channel scattergram is obtained based on these scattered lights, as
shown in the figure below. From the scattergram, the respective proportion of lymphocyte,
monocyte, eosinophil and neutrophil in the total number of WBCs can be obtained.
12
.3.4 Hemoglobin Concentration (HGB) Measurement

In the colorimetric bath, after lyse is added to the diluted sample, the RBCs are dissolved,
and hemoglobins are released. The hemoglobins are combined with the lyse to form hemoglobin
compound. At one end of the colorimetric bath, let the LED light emit monochromatic light with
the wavelength of 550nm to irradiate the hemoglobin compound solution. At the other end, the
PHOTO DIODE(PD) receives transmission light; the optical intensity signal is amplified and
converted to voltage signal which is compared with the voltage produced by the blank
transmission light measured before the sample is added to the colorimetric bath (that is, there is
only diluent in the colorimetric bath) to obtain the HGB of the sample.
.3.5 Red Blood Cell (RBC)/Platelet (PLT) Measurement
The analyzer uses the principle of electrical impedance method to test the number of
RBC/PLT in the RBC test unit. The test unit has a small opening called “test hole(aperture)”. The
hole has a pair of positive and negative electrodes on both sides that are connected to the
constant current power supply. As blood cells have the features of bad conductor, when the
blood cells in the diluted sample pass through the test hole under the constant negative
pressure, the resistance between electrodes will change, thus forms a pulse signal with the size
in proportion to the volume of the cell on both ends of the electrode. When the cells pass
through the hole continuously, a series of electrical pulses will be produced on both ends of the
electrode. The quantity of these electrical pulses reflects the number of blood cells.
Compare the amplified electrical pulses collected with the channel voltage threshold
corresponding to the normal RBC/PLT volumn range, and calculate the number of electrical
pulses in the RBC/PLT channel of the electrical pulse amplitude. Thus, all electrical pulses
collected are classified according to the different channel voltage thresholds, and the number of
electrical pulses in the RBC/PLT channel is the number of RBCs/PLTs. The number of cells in
each channel range divided based on the pulse voltage amplitude decides the volume
13
distribution of cells. The two-dimensional diagram with the x-coordinate representing cell volume
and the y-coordinate representing relative cell quantity is the histogram reflecting the cell
population distribution.
.3.6 Parameters
The parameters are calculated as follows:
 White Blood Cell Quantity (WBC)
The analyzer obtains the WBC quantity by directly measuring the number of electrical
pulses corresponding to WBC.
 Neutrophil Percentage (NEUT%)
 Lymphocyte Percentage (LYM%)

Particle quantity in lymphocyte area in DIFF channel
。
Particle quantity in WBC area in DIFF channel
 Monocyte Percentage (MON%)
Particle quantity in monocyte area in DIFF channel
。
 Eosinophil Percentage (EOS%)
Particle quantity in eosinophil area in DIFF channel
。
 Basophil Percentage (BAS%)
Particle quantity in basophil area in DIFF channel
。
 Neutrophil Quantity (NEUT#)
 Lymphocyte Quantity (LYM#)
 Monocyte Quantity (MON#)
 Eosinophil Quantity (EOS#)
 Basophil Quantity (BAS#)
 Hemoglobin Concentration (HGB)

Background transmission
intensity
Constant
Sample transmission
 Red Blood Cell Quantity (RBC)
intensity
The analyzer obtains the RBC quantity by directly measuring the number of electrical
pulses corresponding to RBC.
 Mean Corpuscular Volume (MCV)
MCV is obtained from the RBC histogram.
 Hematocrit (HCT)
14
 Mean Corpuscular Hemoglobin (MCH)
 Mean Corpuscular Hemoglobin Concentration (MCHC)
 Red Cell Distribution Width - Coefficient of Variation (RDW-CV)

RDW-CV is obtained from the RBC histogram, with the unit of %.
 Red Cell Distribution Width - Standard Deviation (RDW-SD)
RDW-SD is obtained from the RBC histogram, with the unit of fL.
 Platelet Quantity (PLT)
The analyzer obtains the PLT quantity by directly measuring the number of electrical
pulses corresponding to PLT.
 Mean Platelet Volume (MPV)
MPV is obtained from the PLT histogram.
 Platelet Distribution Width (PDW)
PDW is obtained from the PLT histogram. and is a heterogeneity parameter reflecting
the platelet volume, with the unit of fL.
 Platelet Hematocrit (PCT)
 Platelet Large Cell Ratio (P-LCR)

Particle quantity in
platelet large cell area , with the unit of %.
PLT particle quantity
.4 Performance
For performance indicators not specified in this section, they are applicable to both the
Whole Blood and Pre-diluted modes.
 Blank Test
Table 1-2 Blank Test Requirements
Parameter Local Range
WBC ≤ 0.2 x /L
RBC ≤ 0.02 x /L
HGB ≤ 1g/L
PLT ≤ 10 x /L
HCT ≤ 0.5%
 Carry-over
15
Table 1-3 Carry-over
Parameter Carry-over, %
WBC 0.5
RBC 0.5
HGB 0.5
PLT 1
 Repeatability
Table 1-4 Repeatability
Parameter Whole Blood Mode

CV(%) or Absolute Reference Range
Deviation
WBC ≤2 (4-15) x /L
RBC ≤ 1.5 (3.5-6) x /L
HGB ≤ 1.5 (110-180)g/L
MCV ≤1 (70-120)fL
PLT ≤4 (100-500) x /L
MPV ≤4 /
±4 (Absolute (50%-60%) and WBC ≥ 4 x
NEUT% Deviation)
/L

LYMP%
Deviation) /L
MONO%
Deviation) /L
±1.5 (Absolute (2%-5%) and WBC ≥ 4 x /L
EO%
Deviation)
±0.8 (Absolute (0.5%-1.5%) and WBC ≥ 4 x
BASO%
Deviation) /L
 Linearity
Table 1-5 Linearity
Parameter Whole Blood Mode

Measurement Range
(%)
±5% or ±0.3 x
WBC 1-99.9 x /L
/L
±5% or ±0.05 x
RBC 0.3-7 x /L
/L
HGB ±2% or ±2 g/L 20-240g/L
HCT ±3% or ±2HCT% 0-67%
16
PLT ±8% or ±10 x /L 20-999 x /L
.5 Scope of Application
The analyzer is mainly used to test human blood samples, make qualitative and quantitative
analysis of the visible components of blood, and provide the related information. It is suitable for
testing white blood cell quantity (WBC), red blood cell quantity (RBC), platelet quantity (PLT),
hematocrit (HCT), hemoglobin (HGB), and WBC 5-part differentiation. It is used in experiments
made by medical units, inspection units, disease control centers, scientific research institutions,
etc.
.6 Technical Parameters
Testing WBC/RBC/PLT: Electrical impedance method; HGB: Colorimetry;
Principle: WBC 5-part differentiation: Laser scattering method
Measuring
≤ 60sample/hour
Speed:
Display: External PC
Connector: Ethernet port
Work Temperature 15C~30C; RH 30%~85%; Air Pressure 70 kpa-
Environment: 106kpa
Storage
Temperature 0C~40C; RH ≤ 85%; Air Pressure 50 kpa-106kpa
Environment:
Transport Temperature -20℃~55℃; RH ≤ 93%; Atmospheric Pressure 50
Environment: kPa~106 kPa
Mains Input: a.c.100V-240V, 50Hz/60Hz
Input Power: ≤ 600VA
Instrument
Standby ≤ 60dB; Running ≤ 66.86DB
Noise:
.7 PC Configuration
This instrument uses the PC terminal software control instrument, and the operational
software is installed on the PC. The recommended PC configuration is as follows:
CPU: 1.6GHz or above; Memory: 512MB or above, HDD Capacity: 160GB or above. Best
Display Resolution: 1366*768; OS: Microsoft Windows XP or above. The PC has at least 2
network ports (one is used to connect to the instrument, and the other to the LIS/HIS) and 2
USB ports.
Before using the instrument, set the IP address of the PC to the fixed address 192.168.1.64
and the subnet mask to 255.255.255.0. For the specific operations, refer to the Help file of
Windows.
To connect to the LIS, in System Setup – Communication Setup, set the IP address and
port of the LIS. If the LIS is installed on the same PC, the IP address is set to 127.0.0.1. If the
LIS is not installed locally and the IP address is in the same network segment as “192.168.1.64”,
17
the address of the instrument needs to be changed to one not in the same network segment as
“192.168.1.64”, that is, the third number “1” in the address “192.168.1.64” is changed to N (a
number from 2 to 250) (i.e. “192.168.N.64”), and the fourth number “64” can be changed
between 1 and 250.
If the OS is Microsoft Windows XP, in order to get the best display effect of the analysis
software, the Microsoft YaHei font needs to be installed.
18
Chapter 2 Installation
.8 Unpacking
.8.1 Steps of Unpacking
Unpack the instrument and remove the materials for transport. Keep the packing case
and packing materials properly for future repacking.
1) Put the packing case upright and ensure the arrow on the packing case points upward.
 Open the packing case with tools, take out the accessories, and check the items
against the packing list.
2) Take out the upper buffer. At least two persons carefully take the instrument from the
packing case by grasping the carrying handles at the bottom on both sides of the
instrument and put it on a level operation desk.
Caution: The accessories packed should be consistent with the packing

list.
.8.2 Handling Method

 Ensure to securely bind the movable mechanism, such as sampling assy, blending
assy, etc., particularly the sampling probe should be put on the top, before handling.
 For short-distance transport in the smooth and steady condition, a trolley, etc. can be
used.
 During handling and transportation, take care to protect the front board, sampling
probe, and Fluidics connector on the rear side against external force or contact with
other objects or damage.
 During handling and transportation, keep the machine upright, with the angle of
inclination less than 15°, and do not put it on its side.
 Try to avoid vibration during handling. After handling, check and debug the machine
before use.
.9 Installation and Use Environment

This instrument must be installed by professionals. In order to ensure the instrument
works normally, put it in a workplace meeting the following requirements:
 No direct sunshine:
 No large amounts of dust;
 No strong electromagnetic radiation;
 Easy power off operation;
 A level, solid desk that is big enough;
 With good ventilation;
 Avoid moist and high temperature;
 Avoid violent vibration and impact.
Requirements of Use Environment: Normal work environment for the instrument:
Temperature: 15~30℃; RH: 30%~85%.
19
.10 Requirements of Power Supply

 a.c.100V-240V
 50Hz/60Hz
 ≤ 600VA
.11 Requirements of Space

The distance between the right door of the instrument and the wall shall be ≥ 100cm,
and the distance between the rear side of the instrument and the wall shall be ≥ 50cm. Make
sure there is adequate space on the operation desk and under the instrument for the diluent,
cleanser and waste liquid containers. (Ensure the space for repair and maintenance;
consider the heat dissipation of the instrument and protect the Fluidics pipe on the back of
the instrument against extrusion which may impact the normal flow of reagents).
.12 Removal of Auxiliary Fixing Structure for Transportation

Remove the bundles on the movable mechanisms on the sampling assy at 2 positions
(vertical and horizontal belts).
.13 Connection of Reagent and Waste Liquid Containers

1. Connect the reagents to the corresponding reagent connectors on the rear side of
Hemaray 86\89 with the bottle cap assys. The connectors are shown in the figure below.
Table 2-6 Reagent Connector Connection Table
S/N Reagent Machine Connector

Connector Color
1 Diluent RD-86 Diluent Blue
2 Lyse RL-86H 86H Lyse Red
3 Lyse RL-86D 86D Lyse Green
4 Cleanser RC-86 Cleanser Yellow
5 Concentrated Directly sucked from the sampling
Cleanser RC-86C probe
2. Connect to the waste liquid container, and connect the waste liquid connector on the rear
side of the instrument to the waste liquid sensor connector with the bottle cap assy of the
waste liquid container.
20
.14 Connecting Power Supply
1. Connect the Hemaray 86\89 to the patch board or receptacle using power supply cable.
2. Connect the Hemaray 86\89 to PC using LAN cable.
3. Connect the PC to the patch board or receptacle using power supply cable.
4. Connect the printer to the patch board or receptacle using power supply cable and to the
PC using the signal cable.
.15 Preparation before Power-on
Again confirm that all the devices are correctly connected and respectively turn the power
supply outlet switch, Hemaray 86\89 instrument, and PC switch ON. Confirm that the IP
address of the PC is“192.168.1.64”.Install Hemaray 86\89 system software on the PC.
(Default installation address: D:\Program Files\Hemaray. Ensure no other software is
installed and no other files are stored in D: disk.) . After the installation, open the “Automated
Hematology Analysis Software” on the desktop of the PC, and input the username “5x3041”
and the corresponding password. Click “OK” and immediately press the key combination
“ALT+F4” on the keyboard to directly enter the Test interface. The initialization will be
conducted, during which, if the dialog box “The configuration of the PC is inconsistent with
that of the instrument” is pops up, be sure to use the configuration on the instrument. Then
1) Enter “Menu → Service → Mechanical Check” as shown in the following figure:
Check each part. It's important to note that for the syringe and sampling probe tests, after
each part is tested, the software will give prompt. Do not test other parts before the Finish
prompt appears to avoid faults; for the valve test, the valve is normal only when 25 valve
switching sounds are heard, or the valve can be tested one by one.
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2) Enter “Menu → Service → Adjustment → Probe Pos.” to  Detect the various positions
of the sampling probe.
The Hemaray 86/89 interface is as shown in the following figure:
You do not need to conduct any Detect and just confirm the number of steps is the
corresponding value。
Vertically, the value of “Upper Pos.” is 0;
the value of “Isolation Bubble Pos.” is 100;
the value of “Autoloader Sampling Pos.” is 800;
the value of “closed Sampling Pos.” is 800;
the value of “First Piercing Pos.” is 400.
[1] When Detecting the various positions, be sure not to click the “Adjust” button at will,
otherwise, the probe position may be wrong. Please bear this in mind。
[2] Before the Detect, please conduct “Assy Init.”, and doing so is required after each position
is Detected.
[3] Firstly, Detect whether the horizontal position is accurate. For example, to Detect “DIFF
Bath Pos.”, click the “Detect” button under the DIFF bath position and judge whether the
sampling probe assy moves above the DIFF bath. The judgment method is: click the
“V.Motor Power-off” button, move down the sampling probe with hand, and observe whether
the sampling probe can be inserted into the DIFF bath near the middle of the bath. If it can’t
enter or the deviation is big, firstly check whether the horizontal belt is loose. If it is loose,
adjust the belt and conduct the Detect again. Be sure to conduct “V.Motor Power-on”
(corresponding to the aforesaid “V.Motor Power-off”) and then “Assy Init.” before the next
Detect, otherwise, the initialization will go wrong. If the horizontal belt is not loose, the
position of the probe needs to be adjusted. The adjustment method is as follows: horizontally,
click the “Adjust” button under “DIFF Bath Pos.” to pop up “Move the probe to the appropriate
position, and click the ‘OK’ button!” prompt box, and move the sampling probe horizontally
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with hand to the middle of the DIFF bath; when the movement is finished, click “OK” on the
prompt box, and the sampling probe will be reset horizontally; click “Read”; click the “Setup”
button. When the position adjustment is finished, conduct confirmation Detect of the results
of adjustment. For the method, see Steps [2] and [3].
[4] The detect steps of other positions are the same as Step [3].
[5] For vertical detecting, the standard for judging whether the position is correct is: the tip
of the sampling probe is about 2mm away from the bottom of the “Bath” or “Tube”.
For horizontal Detecting, the standard for judging whether the position is correct is:
the sampling probe is near the middle of the bath.
3) Enter “Menu → Service → Adjustment → Autoloader” to Detect the various positions of the
automatic sampler.
 Detecting the resetting position of “Mix Motor”: Click the "Initialize" of “Mix Motor”
and then the grip is at horizontal position, not inclined. When the grip moves upwards, it
must be 2 mm or more from the sampling assembly. Otherwise, adjust the "Reset and
Correct" quantity behind the “Detect Mix Motor”. Click the "Initialize" of this motor and
again conduct Detecting until the requirements are satisfied. Please note that it is
necessary to click "Setup" for effective adjustment of parameters after the resetting and
correction quantity is adjusted.
 “Elevating motor: Detect the resetting position: Push one sample holder to the
Detect tube position. Click the "Initialize" behind the “Elevating Motor” and then
manually pull the grip over the sample holder. It is required that the lower gripping edge
is about 3 mm from the sample. If this condition fails to be met, adjust the parameters
behind the “Elevating Motor” and again Detect until the requirements are met. Please
note that it is necessary to click "Setup" for effective adjustment of parameters after
adjustment.
 Detecting the Detect gripping: Push the sample holder containing two Detect tubes
to the Detect gripping position. Click the "Detect" at this position and then the Detect
tube is just positioned on the center of the grip. When clicking the "Detect" behind the
“Elevating motor”, the Detect tube is able to smoothly leave away and be put into the
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sample holder. Otherwise, adjust the parameters behind the "Pinch Pos.” Click the
"Detect" at this position until the above-mentioned requirements are met. Please note
that it is necessary to click "Setup" for effective adjustment of parameters after
adjustment.
 Detecting other parameters: The defaulted parameters in the above figure are
used for others.
 Detecting scanner position: Push a sample holder containing empty tubes under
the scanning rotary motor. Click the "Detect" behind the "Feeding Steps" and then click
the "Turn on scanner’s Laser". It is required that the light emitted from the scanner is
just directed the center of the Detect tube. If not, adjust the installation position of the
scanner. Upon completion, click the "Turn off scanner’s Laser".
4) Check the pipes connection, confirm there are no pipes detached, enter “Menu → Service
→ Daily Maintenance → Fluidics Maint.”, and conduct “Prime” for the Fluidics respectively.
.16 Debugging
1. Blank Test
Blank Test in Automatic-Whole Blood Mode:
Click “Sample Analysis” on the top of the software interface, click the “Next(N)” button on
the “Sample Analysis” interface, and make the setup shown in the following interface:
Click the “OK” button to enter the Test interface in the Enclosed-Whole Blood mode and
conduct the blank test. If the blank is not up to standard, conduct the blank test again. If it is
still not up to standard, clean the related pipes (the easiest method is: enter “Menu →
Service → Daily Maintenance → Clean” to conduct “Fluidics Clean”), and measure the blank
till the blank meets the requirements listed in Table 2-2.
Blank Test in Enclosed-Whole Blood Mode:
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Click “Sample Analysis” on the top of the software interface, click the “Next(N)” button on
the “Sample Analysis” interface, and make the setup shown in the following interface:
Click the “OK” button to enter the Test interface in the Enclosed-Whole Blood mode and
conduct the blank test. If the blank is not up to standard, conduct the blank test again. If it is
still not up to standard, clean the related pipe (the easiest method is: enter “Menu → Service
→ Daily Maintenance → Clean” to conduct “Fluidics Clean”), and measure the blank till the
blank meets the requirements listed in Table 2-2.
Table 2-7 Blank Indicators
Parameter Blank Value

WBC ≤ 0.2 x 109/L
HGB ≤ 1g/L
HCT ≤ 0.5%
RBC ≤ 0.02 x 1012/L
PLT ≤ 10 x 109/L
2. CBC Gain Test

Enter the “Menu” → “Service” → “Adjustment” → “CBC Gain” interface.
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Conduct sample analysis on the interface with normal-level Quality Control, observe
whether the positions of the “Lymphocyte Peak” and “RBC Peak” are within the target value
deviation range corresponding to the peak given in the Quality Control Target Value Table
and whether “HGB Blank AD” is between 2500 and 2900.
 If “Lymphocyte Peak” is on the large side, decrease the value of “WBC Gain”; if
“Lymphocyte Peak” is on the small side, increase the value of “WBC Gain”.
 If “RBC Peak” is on the large side, decrease the value of “RBC Gain”; if “RBC Peak” is
on the small side, increase the value of “RBC Gain”.
 If “HGB Blank AD” is on the large side, decrease the value of “HGB Gain”; if “HGB Blank
AD” is on the small side, increase the value of “HGB Gain.
3. Standard Particle Test
Enter the “Menu” → “Service” → “Adjustment” → “DIFF Gain (Particle)” interface.
26
Dilute 7um standard particles into liquid with the total quantity of 3000~5000 for testing.
Requirements: the CV values of FS and FL are below 7%.
If the CV value of FS or FL does not meet the requirements, the inner and outer walls of
the sheath flow cell need to be cleaned.
Note: The method for diluting standard particles is: add about 1ml deionized water to the
bullet-shaped container and then add 2 drops of standard particles, and blend them for
testing.
4. Fresh Blood Test
Enter the “Menu” → “Service” → “Adjustment” → “DIFF Gain (Blood)” interface.
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Use at least 3 tubes of normal fresh blood for testing. The requirements are as follows:
Table 2-8 Table of Requirements of Centers of Gravity of Fresh Blood
LOWER
PARAMETER LIMIT UPPER LIMIT
CENTER OF
NEU ON FS 612 676
CENTER OF
LYM ON FL 309 341
CENTER OF
NEU ON SS 255 345

If the Center on FS is out of the range: when the value is on the low side, increase the
FS gain slightly; when the value is on the high side, decrease the FS gain slightly.
If the Center on FL is out of the range: when the value is on the low side, increase the FL
gain slightly; when the value is on the high side, decrease the FL gain slightly.
If the Center on SS is out of the range: when the value is on the low side, increase the
SS gain slightly; when the value is on the high side, decrease the SS gain slightly.
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.17 Software Installation and Upgrade

.17.1 Installation of XP OS
1. Install the English or Chinese version OS according to the requirements of the contract
review.
2. Install the Sogou pinyin input method and WNWB input method to the default path (for
Chinese version OS only).
3. Enter the “Control Board” menu of the system and select “Date, Time, Language, and
Regional Options”. Click “Change Number, Date and Time Formats” and enter “Regional and
Language Options”. Click “User-defined” on the right side. On the “Number” menu, select the
“0.7” format in “Display leading zeros”, and click “Apply” and “OK” to exit.
4. Double-click the “Local Area Connection 2” on the bottom right corner of the interface
(Figure 1), click “Attribute”, and select “Internet Protocol (TCP/IP)”; click “Attribute”, and set
the IP address to: 192.168.1.64 and the subnet mask to 255.255.255.0. When the setup is
finished, click “OK” to exit the Setup interface.
5. Install the printer driver to the default path.
.17.2 Installation of Information Processing System Software
1. Copy the whole folder of the Hemaray 8X Automated Hematology Analyzer software under
the root directory of D: disk.
2. Open the Hemaray 8X Automated Hematology Analyzer software, double-click the “setup”
file in the folder, click “Next”, and click “Next” again.
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3. Click the “Browse” button, create a Hemaray folder under the root directory of D: disk, and
select the new folder as the installation directory. Click the “Next” button, and the program will
begin to install the software till the installation is ended.
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4. Click the “Finish” button to finish the installation, and an “Automated Hematology Analyzer”
shortcut will be created on the desktop.
.17.3 Installation of Main Control Board System Program
.17.3.1 Programming FPGA
FPGA Programming Tool: USB-Blaster Connection Method: Connect the USB port to the PC,
and the other to the port J5 on the main control board.
1. Click “Start” and, from the “Programs” on the “Start” menu, run the programming software
(Quartus II 12.osp2 Programmer), as shown in the figure below:
2. On the window popped up, click “Hardware Setup”, as shown in the figure below:
3. From the “Currently selected hardware” option, select “USB-Blaster[USB-0]” and click
“Close”.
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4. From the “Mode” option, select “Active Serial Programming” and click “Add File”. On the
window popped up, find the folder where the file to be programmed is located.
5. Double-click the file to be programmed.
6. On the window popped up, tick the options shown in the above figure according to the
prompt and click “Start” to begin programming.
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.17.3.2 Programming QuickLoader

1. Run lpc32x0Loader.exe and select to browse the quickload.hex file;
2. Execute Command: :>sload 0x80000000 (download quickload.hex to LPC3250 SRAM and
directly execute the quickload.hex file); select the quickload.bin file (files with .bin extension
can’t be executed directly and can be executed after reset and restart);
3. Execute Command: :>nand writebooter 0x80000000 0xe000;
4. Reset, and the main control board can be started from nandflash.
.17.3.3 Updating Eboot
1. Run lpc32x0Loader.exe and click the "Enter the terminal directly” and "Begin connection"
buttons.
2. Type “sload 0x80000000” (off-chip SDRAM) at the command prompt. When you are
prompted to update the file, click the “Send file over serial port” button, select EBOOT.nb0,
and download the file to the address 0x80000000 in SDRAM.
3. When the downloading is finished, type “nand write 0x80000000 0x40000 0x40000” to
write the data in SDRAM to the address 0x40000 in FLASH (to command the former), with
the file size of 0x40000 (to command the later).
4. Open “option show” to check.
5. Type the following commands and parameters:
option set AutoCall yes; option set CallDelay 2000; option set CallRunAddr 0x83fc0000;
option set CallDataAddr 0x40000; option set CallDataLen 0x40000; option save.
6. You can type the “option show” command to check whether the parameters have been set.
7. When the system prompts that the writing is finished, the update is ended. Restart the
main control board.
.17.3.4 Updating WinCE Core
1. Connect the main control board to the PC with the serial port cable, open the COMPort
Debugger, select to directly enter the terminal, and set the baud rate to 115200.
2. Copy the nk.bin file to the SD card, insert the SD card into the main control board, and
reset the main control board.
3. Enter QuickLoader and it will start automatically.
4. Enter Eboot to start it. Wait and press the 2nd space key to enter the menu for selection.
5. Select “4. Select boot device”.
6. Select Submenu 2 and SD card starting mode. Here, the system will ask you to input the
file name. Input "nk.bin" and press Enter.
7. Press 0 to exit the QuickLoader setup.
8. Here, the core will be updated from the SD card automatically.
.17.4 Installation of Main board application Software
.17.4.1 Initial Software Installation
After the main board application system is installed, copy the software MyApp.exe and
AutoUpdate.exe relating to the main board application to My Device -> Flash Storage through
the SD card. For operation, the display needs to be connected to be used with the mouse or
touch screen.
.17.4.2 Upgrade Main board application Software
1. Login the system with production authority, enter Service -> Software Upgrade, and open
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the dialogue box shown below:
2. Select the Main board application Software to be upgraded with the “。。。” button,
check that the PC and the instrument have been connected to the network, and click the
“Download” button. The progress bar will prompt the downloading progress. After the file is
downloaded, the Main board application Software will be turned off automatically, and the
new version software will run again. You can check the version information of the main board
application in Service -> Version Information.
.17.5 Installation of Driver Board Program

Tool: U-EC6 programmer for MCU
Description: On the driver board, the model of MCU used is: C8051F340; the number of
MCUs is 5; the corresponding software names are MAIN, MCU1, MCU2, MCU3, and MCU4;
the programming software for MCU C8051F340 is MCUProductionProgrammer.exe; the
software number is RT-DTO-001.
1. JP1 and JP2 Configuration
When programming MCU1: Pins 3 and 4 of JP1 and Pins 3 and 4 of JP2 are connected to
jumpers. When programming MCU2: Pins 9 and 10 of JP1 and Pins 9 and 10 of JP2 are
connected to jumpers. When programming MCU3: Pins 1 and 2 of JP1 and Pins 1 and 2 of
JP2 are connected to jumpers. When programming MCU4: Pins 7 and 8 of JP1 and Pins 7
and 8 of JP2 are connected to jumpers.
When programming MAIN: Pins 5 and 6 of JP1 and Pins 5 and 6 of JP2 are connected to
jumpers.
2. Connect the instrument and the USB port on the PC with the download cable.
3. On the PC, open the download program MCUProductionProgrammer.exe.
4. Click the menu option Program Menu -> Configure Programming Information…, as shown
in the figure below:
34
5. In the download setup, click the dropdown box “Part” and select “C8051F340” from the
dropdown box. In the “Settings” check box, select the “Erase code space before
programming” option. In the “Hex File Location(s)” option box, click the “Open File” button on
the top right corner. Select to open the BootLoad.hex boot loader file. Configure
Programming Settings are shown in the figure below:
Note: After the “Configure Programming Settings” dialogue box is opened, when the “Debug”
option box is not in the format of TSxxxxxxxx (such as TS00014857), it indicates that the
download cable is not connected properly. Here, you should close
MCUProductionProgrammer.exe, reconnect the USB properly, and open
MCUProductionProgrammer.exe again for setup.
6. After the Configure Programming Settings are finished, click “Accept Settings” botton to
return to the main interface of the downloader, shown in the figure below:
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7. On the main interface of the downloader, click the “Program Device” button to begin to
download the boot loader. When the programming prompt box displays “Device Erased,
Programmed and Verified”, it indicates the programming is finished. Turn off the power of the
board and unplug the download cable from the board.
.17.6 Installation of Automatic Sampler Driver Board Program

Description: The installation of automatic sampler driver board program is basically
consistent with that of driver board program, and the main difference is the jumper
configuration. For the steps of programming, see Section 2.10.5.
JP1 and JP2 Configuration:
When programming MAIN: Pins 1 and 3 of JP1 and Pins 2 and 4 of JP1 are connected to
jumpers. When programming CPU1: Pins 3 and 5 of JP1 and Pins 4 and 6 of JP2 are
connected to jumpers.
.17.7 Installation of Fluidics Sequence
1. Click the “Menu” button on the top left corner of the main interface, and click “Service →
Sequence File Update” to enter the “Sequence File Download” interface.
2. Click the “。。。” button and select the folder where the sequence file is located.
3. Click the “OK” button, select the TIMING.RTM format file, click the right arrow, and move
the file to the pending download section on the right side.
4. Click the “Download” button to begin the sequence file update. When the update is
finished, the software will prompt “File downloading finished”.
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37
Chapter 3 Hardware System
According to the mechanical molding design and the whole machine layout design, its
overall hardware structure is divided into three assys, i.e. electrical circuit unit, power supply
assy, and shell assy.
.18 Position of Electrical Circuit Unit

Including main control board, driver board, burning board, sampling assy interface
board, sampling assy adapter board, laser driver board, forward small-angle
preamplification board, forward large-angle preamplification board, side scatter
preamplification board, etc. The main control board and driver board are located in the
space on the back of the whole machine. The sampling assy interface board and sampling
assy adapter board are located on the sampling assy. The laser driver board, forward
small-angle preamplification board, forward large-angle preamplification board, and side
scatter preamplification board are located in the optical path assy. The arrangements and
actual positional relations of the boards are shown in the figure below:
Driver board
Main control
board
38
Burning board
Side scatter Forward large-angle

preamplification board preamplification
Laser driver board board
Forward small-angle
preamplification
board
Sampling assy
Sampling assy interface board
adapter board
39
Figure 3-1 View of Instrument
The boards are directly fixed to the corresponding position on the respective assy with
screws. Seven M3 screws are used for the driver board; six for the main control board; four
for the burning board; three for the sampling assy adapter board; four for the sampling assy
Interface board; four for the laser driver board, forward small-angle preamplification board,
forward large-angle preamplification board, and side scatter preamplification board; four
screws are used for the automatic sampling driver board. There is a partition between the
main control board and the driver board as a shield.
.19 Power Assy Section

The power assy section includes one 24V power module, one 5V power module, filter,
power switch, fuse holder, fuse, etc. The arrangements are shown in the figure below:
40
24V power
5V power
Fuse holder &

fuse
Power switch
Filter
Figure 3-2 Power Module
.20 Shell Assy Section

The shell assy section includes LED display, network interface board and 3 microswitches
which show closing state of the left door,right door and front door respectively. The
arrangements are shown in the figure below:
Left door closing

microswitch
LED display
board
Key board
Right door closing

microswitch
Front door closing

microswitch Network interface
board
Fgirue 3-3 Shell Assy
.21 Main Control Board

.21.1 Basic Functions and Module Division
41
1) Embedded Platform
The embedded platform uses ARM9 as the core. The peripheral circuits include real-time
clock (RTC), SDRAM, NAND FLASH, Ethernet, and UART circuits. In which, ARM9, SDRAM,
and NAND FLASH constitute the basic running platform of the software; RTC provides the
calendar and real-time clock; Ethernet provides the 10/100Mbps adaptive network; UART
provides the RS-232C circuit.
2) Digital Logic Processing Unit.
The digital logic processing unit uses FPGA as the core. The auxiliary circuit includes
serial FLASH download and 2.5V DC-DC power supply circuits. In which, the serial FLASH
mainly stores the FPGA logic; the 2.5V DC-DC power supply provides 2.5V DC current to the
FPGA phase-locked loop circuit.
3) Analog Conditioning and ADC
Analog conditioning and ADC is divided into DIFF and CBC analog conditioning. In
which, the DIFF conditioning is divided into blocking, buffer, digital gain adjustable amplifying,
active power filter, and buffer and pipelined ADC processing circuits, while the CBC
conditioning is divided into blocking, one-stage amplifying, two-stage amplifying, RC filter,
three-stage digital gain adjustable amplifying, buffer, and pipelined ADC circuits.
.21.2 Principle
The specific functional block diagram is as follows:
.21.3 PCB Layout Diagram
42
.21.4 Connector Pin Assignment
J1: LCD Output (LCD_IF);

PIN No. Assignment Description
1 LCDVD17 LCD data signal 17
9 GND Digital ground wire
18 +3.3V +3.3V power supply
43
20 PWR LCD power supply enable signal

21 VS LCD frame signal
22 LE LCD line end signal
27 PCLK LCD pixel clock signal
34 HS LCD line signal
39 BLPWM LCD backlight PWM adjustable signal
40 DE LCD display enable signal
J2: Universal Serial Bus Communication (USB2~4);

1 DM2 USB2 differential signal D-
2 VBUS2 USB2 power supply
3 DP2 USB2 differential signal D+
J3: Touch Screen Input (Touch_IF);

1 YP Touch screen position Y + analog signal
2 XP Touch screen position X + analog signal
4 AUX_IN Auxiliary analog signal of touch screen
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5 XM Touch screen position X - analog signal

6 YM Touch screen position Y - analog signal
J4: Serial Port Communication (UART_3,6);

1 RS232_U3_TX Send signal through serial port 3 (RS-
232C level)
3 RS232_U3_RX Receive signal through serial port 3
(RS-232C level)
4 RST# Reset signal
5 NC Blank
6 NC Blank
232C level)
(RS-232C level)
10 RST# Reset signal
J7: Forward Small-angle (FS) Signal Input;

1 +12VA +12V power supply
2 AGND Analog ground wire
3 -12VA -12V power supply
4 FS Forward small-angle signal input
J8: Forward Large-angle (FL) Signal Input;

4 FL Forward large-angle analog signal
J9: Side Scatter (SS) Signal Input;

45

4 SS Side scattering angle analog signal
J11: White Blood Cell (WBC) Signal Input;

1 WBC_IN- WBC negative electrode analog signal
2 WBC_IN+ WBC positive electrode analog signal
J12: Red Blood Cell and Platelet (RBC/PLT) Signal Input;

1 RBC_IN- RBC negative electrode analog signal
2 RBC_IN+ RBC positive electrode analog signal
J13: Hemoglobin Signal Input (HGB);

1 V_DIODE HGB LED constant-current light power
supply
3 HGB HGB analog signal
J14: +5V,+24V Power Entry (Power +5V,+24V);

1 +5V +5V power supply
6 PGND Power ground wire
J15: Power Supply Software Controllable On/Off Signal Output
(PWR_SW)；
1 PWRDWN When the software is shut down, the
power supply Off signal is controlled.
2 NC Blank
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J16: Laser Software Controllable On/Off Signal Output and Current
Detection Input (LASER);

3 +12VALD +12V laser power supply
5 Vild Laser current signal
6 Vipd(ld) Laser current PHOTO DIODE(PD)
detection signal
8 SW_LD Laser software On/Off signal
9 LED_LASER Indication signal of laser indicator
J17: Key Detection Input (DETECT);

1 DET_L Left door detection signal
3 DET_R Right door detection signal
5 DET_C Front cover detection signal
7 KEY_CUT Test key signal
9 NC Blank
J18:LED Display Output and Automatic Sampling Key Input
(LED&AUTO_KEY);
5 LED_RUNNING Indication of normal system running of
LED indicator
7 LED_WARNING Indication of system warning of LED
indicator
47
9 KEY_TEST Test key testing (for automated model)

11 KEY_OCW Open/Close Sample compartment door
key testing (for automated model)
J19: Parallel Port and Serial Port Communication LPT&UART;

1 PT_AFD# Extended parallel port printing word-
wrap signal
2 PT_D0 Extended parallel port printing data
signal D0
signal D1
signal D2
signal D3
signal D4
signal D5
signal D6
signal D7
10 PT_ACK# Extended parallel port printing response
signal
11 PT_BUSY Extended parallel port printing busy
signal
12 PT_PE Extended parallel port printing end
signal
13 PT_SLCT Extended parallel port printing selection
signal
14 PT_STB# Extended parallel port printing gating
signal
15 PT_ERR# Extended parallel port printing error
signal
16 PT_SLIN# Extended parallel port printing selective
input signal
17 PT_INIT# Extended parallel port printing interrupt
signal
48

28 RS232_STU0_SOUT Send signal through extended serial
port 0 (RS-232C level)
30 RS232_STU0_SIN Receive signal through extended serial
32 RS232_STU1_SOUT Send signal through extended serial
34 RS232_STU1_SIN Receive signal through extended serial
J20: Barcode Scan Communication (SCANNER(UART_1));

2 SOUT1+ Serial digital output signal 1
3 PWRGND Power isolation ground wire
4 SOUT2+ Serial digital output signal 2
5 NC Blank
6 TRIG2 Trigger signal 2
7 NC Blank
232C level)
9 NC Blank
(RS-232C level)
J22: Burning Control Output (BURN);

1 FIRE Burning function switching On/Off signal
3 WORK Burning power On/Off signal
J24: Serial Port Communication (UART_2,5);

49

232C level)
2 GND Ground wire
(RS-232C level)
4 RST# Reset signal
5 NC Blank
6 NC Blank
232C level)
8 GND Ground wire
(RS-232C level)
10 RST# Reset signal
P5: Network Port Communication (NET_IF);

1 TD+ Send differential signal TD+
2 TD- Send differential signal TD-
3 CTT Network transformer node
4 CHS_GND1 Digital ground wire
5 CHS_GND2 Digital ground wire
6 CTR Network transformer node
7 RD+ Receive differential signal RD-
8 RD- Receive differential signal RD-
9 LED_LINK_VCC Connect to activation signal indicator’s
positive electrode
10 LED_LINK Connect to activation signal indicator’s
negative electrode
11 LAN_LED Connect to signal indicator’s negative
electrode
12 LAN_LED_VCC Connect to signal indicator’s positive
electrode
13 SHIELD1 Frame ground wire
.22 Optical System Related Boards

.22.1 Forward Small-angle Preamplification Board
The forward small-angle preamplification board collects forward
50
small-angle signals from the optical path and converts weak current
signals to analog voltage signals recognizable by CPU. Its principle is
shown in the figure below:
Its PCB layout diagram is shown in the figure below:
Connector Pin Assignment
J1:FS Analog Signal Output:

3 -12V -12V power supply
4 FS Forward small-angle analog signal
.22.2 Forward Large-angle Preamplification Board
51
The forward large-angle preamplification board collects forward
large-angle signals from the optical path and converts weak current
Connector Pin Assignment
J1:FL Analog Signal Output:

4 FL Forward large-angle analog signal
.22.3 Side Scatter Preamplification Board
52
The side scatter preamplification board collects side-angle
scatter signals from the optical path and converts weak current
J1:SS Analog Signal Output:

4 SS Side scatter angle analog signal
.22.4 Laser Driver Board
1. Basic Functions and Module Division
(1) Laser Slow Start: As an impulse current is produced when the power switch is turned on,
53
in order to prevent such current from destroying the laser diode, the driving source of the
laser must be designed as Slow Start. The circuit is mainly composed of 2 NPN triodes and
multiple capacitors, and the voltage increases slowly through capacitor charging and triode
turn-on.
(2) Constant-current Driving: The light output of laser diode relates to the drive current. Laser
is produced only when the drive current passing through the laser is above the threshold.
(3) Automatic Power Control (APC): In the constant-current driving mode, the temperature
changes influence the laser output. APC circuit must be added to reduce the influence of
temperature on the output to a certain extent. The circuit is mainly composed of the reference
voltage output, signal amplification, etc. The laser output must be stabilized through
regulation of the drive current according to the signal value at the feedback end of the laser
diode.
(4) Current Detection: For more convenient debugging, the current detection function is
added.
2. Functional Block Diagram of Laser Driver Board
54
3. PCB Layout Diagram of Laser Driver Board
4. Connectors on Laser Driver Board
Connector between Laser Driver Board and Main Control Board
Main Control Board Laser Driver Board Meaning of Signal
J1 (B9B-PH –K-S, JST)
1 (12VA) Analog voltage source 12V
2 (AGND) Analog voltage source GND
3 (12V) Laser voltage source 12V
4 (GND) Laser voltage source GND
5 (LD_CURT) Drive current detection
terminal
6 \ \
55
7 (GND) Analog voltage source GND
8 (SWITCH) Laser control switch
9 \ \
Connector between Laser Driver Board and Laser
Laser Laser Driver Board Meaning of Signal
(welded) J2 (welded)
1 (PHOTO DIODE(PD)) Laser current input terminal
2 (GND) GND
3 (LD) Laser feedback current
output terminal
Connector between Laser Driver Board and Indicator Board
Indicator Board Laser Driver Board Meaning of Signal
J3 (B2B-PH –K-S, JST)
1 1(12V) Indicator power supply
2 2(Signal) Work status indication
control signal
5. Main Test Points of Laser Driver Board
Test Points of Laser Driver Board
S/N Position No. of Test Description
Point
1 LD_CURT Test point of laser drive current,
Current = LD_CURT Potential/50/0.76
.23 Power Driver Board

.23.1 Basic Functions
The driver board realizes the control of all stepper motors, valve pump control and
56
temperature control as well as detection of input signals of position optocoupler, liquid
optocoupler, temperature sensor, etc.
The driver board is functionally divided into three sections:
 Control Section: Composed of 5 MCUs, for instruction parsing and control
 Interface Section: Including Interface of sensor signal input, drive circuit, and control
section
 Drive Section: Composed of stepper motor drive and pump valve drive
.23.2 Functional Block Diagram

The functional block diagram of the driver board is shown in the figure below.
The driver board is composed of 5 MCUs, one of which is used as the dispatching center
and the other 4 are used as parts control software to form a master-slave structure.
.23.2.1 Control Section

 The main control unit is composed of a MCU (U15). Its main function is to receive
commands from the main board application, issue the commands to the parts
control software units, and return the execution results to the main board
application;
 MCU1 (U19) realizes heating and temperature measurement;
 MCU2 (U20) realizes control of sampling syringe motor, diluent syringe motor,
Waste Syringe motor and V23 valve and testing of 1 air pressure sensor;
 MCU3 (U22) realizes control of level motor, vertical motor, valve, and pump of
sampling assy for lyse syringe;
57
 MCU4 (U23) realizes control of sample syringe motor, sheath fluid syringe motor
and sample syringe motor and testing of 5 liquid sensors, 1 liquid pressure sensor,
and 1 waste liquid level.
.23.2.2 Connector Section

 IO Expansion: The CPU for the control section accesses the FPGA through the bus
and realizes expansion of digital signals;
 Optocoupler Sensor Testing: Optocoupler signals after shape correction are input to
the IO port of the FPGA and provide access by the corresponding CPU;
 Liquid Sensor Testing: Signals after processing are input to the analog input port of
the corresponding CPU;
 Air Pressure Sensor and Liquid Pressure Sensor Testing: Signals after processing
and amplification are input to the analog input port of the corresponding CPU.
.23.2.3 Drive Section

 Stepper Motor Drive: Each stepper motor drive uses one IC for independent drive.
 Pump Valve and Heating Film Drive: Provides at least 1.5A current drive.
.23.3 Connector Assignment

Interface Designation Pin No. Designation
No.
J38 Valves 21to 40 1 Power Supply, 24 V
2 V21 Control Signal
3 Power Supply, 24 V
58
J37 Valves 1to 20 1 Power Supply, 24 V
2 V1 Control Signal
59
4 V2 Control Signal
6 V3 Control Signal
8 V4 Control Signal
60
J29 Heating Membrane 1 Control Signal
J16 Pump 1 Control Signal
J36 Power Supply 1 5V
2 24V
3 24V
4 24V
5 GND
6 GND
7 GND
8 GND
AP1 Air pressure
J24 Hydraulic pressure sensor 1 5V
2 Signal-
3 GND
4 Signal+
J33 Waste Liquid Sensor 1 5V
2 3.3V
3 GND
4 Signal
J27 DIF liquid sensor 1 5V
2 3.3V
3 GND
4 Signal
61
J26 HGB liquid sensor 1 5V
2 3.3V
3 GND
4 Signal
J25 DIL liquid sensor 1 5V
2 3.3V
3 GND
4 Signal
J21 Waste Liquid Sensor 1 5V
2 3.3V
3 GND
4 Signal
J11 Serial port, connected to 1 RXD
the main control board 2 NC
3 TXD
4 GND
J17 DIF temperature sensor1 1 GND
2 Signal
3 3.3V
J39 Environmental temperature 1 GND
sensor 2 2 Signal
3 3.3V
J10 Optical coupler Sensor 1 Optical coupler signal of
diluent syringe
2 GND
3 5V
4 Optical coupler Signal of
Sheath Liquid Syringe
5 GND
6 5V
62
Sample Syringe
8 GND
9 5V
Waste Fluid Syringe
11 GND
12 5V
Lyse Syringe
14 GND
15 5V
Sampling Syringe
17 GND
18 5V
Horizontal Motor of
Sampling Assembly
20 GND
21 5V
22 NC
J13 Optical coupler 1 of Sensor 1 Optical coupler Signal 1 of
of Sampling Assembly Vertical Motor of Sampling
Assembly
2 GND
3 5V
J14 Optical coupler 2 of Sensor 1 Optocoupler Signal 2 of
of Sampling Assembly Vertical Motor of Sampling
Assembly
2 GND
3 5V
63
J1 Motor of Diluent Syringe 1 Motor Signal A+
2 Motor Signal A-
3 Motor Signal B+
4 Motor Signal B-
J2 Motor of Sheath Liquid 1 Motor Signal A+
Syringe 2 Motor Signal A-
3 Motor Signal B+
4 Motor Signal B-
J5 Motor of Sampling Syringe 1 Motor Signal A+
2 Motor Signal A-
3 Motor Signal B+
4 Motor Signal B-
J6 Motor of Waste Syringe 1 Motor Signal A+
2 Motor Signal A-
3 Motor Signal B+
4 Motor Signal B-
J3 Motor of Lyse Syringe 1 Motor Signal A+
2 Motor Signal A-
3 Motor Signal B+
4 Motor Signal B-
J4 Motor of sample Syringe 1 Motor Signal A+
2 Motor Signal A-
3 Motor Signal B+
4 Motor Signal B-
J7 Horizontal Motor of 1 Motor Signal A+
Sampling Assembly 2 Motor Signal A-
3 Motor Signal B+
4 Motor Signal B-
J8 Vertical Motor of Sampling 1 Motor Signal A+
2 Motor Signal A-
64
Assembly 3 Motor Signal B+
4 Motor Signal B-
.23.4 Main Test Points

Silk Screen at Designation
Measuring
Points
VCC Power Supply, 5V
24V Power Supply, 24V
3.3V Power Supply, 3.3V
GND GND
TXD Serial port TXD
RXD Serial port RXD
DI-CLK Pulse of Motor of Diluent
Syringe
DI-DIR Direction of Motor of Diluent
Syringe
SH-CLK Pulse of Motor of Sheath
Liquid Syringe
SH-DIR Direction of Motor of Sheath
Liquid Syringe
SA-CLK Pulse of Motor of Sampling

Syringe
SA-DIR Direction of Motor of
Sampling Syringe
WA-CLK Pulse of Motor of Waste
Syringe
WA-DIR Direction of Motor of Waste
Syringe
HE-CLK Pulse of Motor of Lyse
Syringe
HE-DIR Direction of Motor of Lyse
Syringe
65
AS-CLK Pulse of Horizontal Motor of

Sampling Assembly
AS-DIR Direction of Horizontal Motor
of Sampling Assembly
HO-CLK Pulse of Horizontal Motor of
Sampling Assembly
HO-DIR Direction of Horizontal Motor
of Sampling Assembly
VE-CLK Pulse of Vertical Motor of
Sampling Assembly
VE-DIR Direction of Vertical Motor of
Sampling Assembly
.24 High Voltage Switchboard

(1) Power Control: Mainly includes optocoupler isolation and MOS control circuits. When the
burning is not used, the power is isolated to prevent interfering signals.
(2) DC-DC Conversion: With the DC-DC chip as the core, including peripheral booster circuit.
The main working principle is to realize DC 110V constant voltage output through
modulation of pulse width.
(3) Output Control: Mainly includes relay, optocoupler isolation, and MOS control circuits.
Switching between high voltage burning and signal transmission is realized through the
output control circuit.
.24.2 Functional Block Diagram of High Voltage Switchboard
66
.24.3 PCB Layout Diagram of High Voltage Switchboard
67
.24.4 Connectors on High Voltage Switchboard
Connector between High Voltage Switchboard and Power Supply
Power Supply High Voltage Switchboard Meaning of Signal
J6 B4B-PH-KS
1 GND \
5V Power Supply
2 5V \
3 GND \
24V Power Supply
4 24V \
Connector J5 between High Voltage Switchboard and Main Control Board
Main Control Board High Voltage Switchboard Meaning of Signal
J5 B3B-PH-KS
1 1 FIRE Power control signal
2 2 GND GND
3 3 WORK High voltage output control
signal
J2 B3B-XH-A JST
1 WBC_OUT- WBC signal negative

1
electrode
2 2 WBC_OUT+ WBC signal positive
electrode
3 3 SGND Signal shield ground wire
68
J4 B3B-XH-A JST
1 RBC_OUT- RBC signal negative

1
electrode
2 2 RBC_OUT+ RBC signal positive
electrode
Connector between High Voltage Switchboard and WBC Bath
J1 B3B-XH-A JST
1 WBC_IN-/GND WBC signal negative

1
electrode /GND
2 2 WBC_IN+/110V WBC signal positive
electrode /110V
Connector between High Voltage Switchboard and RBC Bath
J3 B3B-XH-A JST
1 RBC_IN-/GND RBC signal negative

1
electrode /GND
2 2 RBC_IN+/110V RBC signal positive
electrode /110V
.24.5 Main Test Points of High Voltage Switchboard
Test Points of High Voltage Switchboard
S/N Test Point Position No. Description
1 TP4 110V test point
69
.25 Autoloader board

1) Numerical Control Unit:
Adopts one master MCU, expands one slave MCU through communication and expands
timer resources, console port I/O, etc.
2) Motor Drive Unit:
Adopts the stepper motor drive chip to realize motor drive.
.25.2 Principle
.25.3 PCB Layout Diagram
70
.25.4 Connector Pin Assignment
J1: Universal Serial Bus Communication (USB);

1 VBUS1 USB power supply
2 D1- USB differential signal D-
3 D1+ USB differential signal D+
1 MAGOUT Electromagnet power drive
1 HOME_1 Optocoupler 1 reset signal
2 OPT1_SPY Optocoupler 1 transmitting terminal
power supply
power supply
71

power supply
power supply
power supply
23 HOME_E8 Optocoupler E8 reset signal
25 HOME_E9 Optocoupler E9 reset signal
J5: Motor Position Detection Input;

1 HOME_01# Optocoupler 01# reset signal
2 OPT01#_SPY Optocoupler 01# transmitting terminal
power supply
power supply
power supply
72
power supply
power supply
power supply
power supply
power supply
power supply
J6: Serial Port 1 Communication;

1 PC_RXD0 Receive signal through serial port 0
(RS-232C level)
4 PC_TXD0 Send signal through serial port 0 (RS-
232C level)
J7: Serial Port 2 Communication;

1 PC_RXD1 Receive signal through serial port 1
(RS-232C level)
4 PC_TXD1 Send signal through serial port 1 (RS-
232C level)
73
J8:+24V Power Entry;

J9: Blending Up/Down Motor (SNATCH);

1 OUT1A Blending up/down motor 1-phase A-end
power drive
2 OUT1B Blending up/down motor 1-phase B-end
power drive
3 OUT2A Blending up/down motor 2-phase A-end
power drive
4 OUT2B Blending up/down motor 2-phase B-end
power drive
J10: Code Scan Up/Down Motor (UPDOWN_M);

1 OUT1A Code scan up/down motor 1-phase A-
end power drive
2 OUT1B Code scan up/down motor 1-phase B-
end power drive
3 OUT2A Code scan up/down motor 2-phase A-
end power drive
4 OUT2B Code scan up/down motor 2-phase B-
end power drive
J11: Blending Front/Back Motor (LR_M_FB);

1 OUT1A Blending front/back motor 1-phase A-
end power drive
2 OUT1B Blending front/back motor 1-phase B-
end power drive
3 OUT2A Blending front/back motor 2-phase A-
end power drive
4 OUT2B Blending front/back motor 2-phase B-
end power drive
J12: Introduction Propulsion Motor (RBF_M);

1 OUT1A Introduction propulsion motor 1-phase
A-end power drive
2 OUT1B Introduction propulsion motor 1-phase
74
B-end power drive

3 OUT2A Introduction propulsion motor 2-phase
A-end power drive
4 OUT2B Introduction propulsion motor 2-phase
B-end power drive
J13: Sampling Propulsion Motor (LR_M);

1 OUT1A Sampling propulsion motor 1-phase A-
end power drive
2 OUT1B Sampling propulsion motor 1-phase B-
end power drive
3 OUT2A Sampling propulsion motor 2-phase A-
end power drive
4 OUT2B Sampling propulsion motor 2-phase B-
end power drive
J14: Code Scan Circle Motor (CIRCLE_M);

1 OUT1A Code scan circle motor 1-phase A-end
power drive
2 OUT1B Code scan circle motor 1-phase B-end
power drive
3 OUT2A Code scan circle motor 2-phase A-end
power drive
4 OUT2B Code scan circle motor 2-phase B-end
power drive
J15: Recycle Propulsion Motor (LBF_M);

1 OUT1A Recycle propulsion motor 1-phase A-
end power drive
2 OUT1B Recycle propulsion motor 1-phase B-
end power drive
3 OUT2A Recycle propulsion motor 2-phase A-
end power drive
4 OUT2B Recycle propulsion motor 2-phase B-
end power drive
J16 (Not in Use) STANDBY;

1 OUT1A Motor 1-phase A-end power drive
2 OUT1B Motor 1-phase B-end power drive
3 OUT2A Motor 2-phase A-end power drive
75
4 OUT2B Motor 2-phase B-end power drive
J17: Tube Blending Motor (SHAKE_M);

1 OUT1A Tube blending motor 1-phase A-end
power drive
2 OUT1B Tube blending motor 1-phase B-end
power drive
3 OUT2A Tube blending motor 2-phase A-end
power drive
4 OUT2B Tube blending motor 2-phase B-end
power drive
.26 Other Boards

.26.1 LED Display
1. Basic Functions
Uses the LED lighting sets and indicates the work status of the
instrument through the LED display.
2. Principle
3. PCB Layout Diagram
76
4. Connector Pin Assignment
J1:LED Display Control Output;

5 LED_RUNNING Indication of normal system running of
LED indicator
7 LED_WARNING Indication of system warning of LED
indicator
11 KEY_OCW Open/Close sample compartment door
J2: Key Signal Switch Input

77

.26.2 Network Port Board
1. Basic Functions
Switches network signals.
2. Principle
J1: Network Differential Signal Switch Input;

1 TD+ Send differential signal TD+
2 TD- Send differential signal TD-
78
3 RD+ Receive differential signal RD+

4 RD- Receive differential signal RD-
P1: Network Communication Port;

1 TX+ Send differential signal TX+
2 TX- Send differential signal TX-
3 RX+ Receive differential signal RX+
4 NC Blank
5 NC Blank
6 RX- Receive differential signal RX-
7 NC Blank
8 NC Blank
9 H1 Hole 1
10 H2 Hole 2
11 EARTH Shell ground wire
12 EARTH Shell ground wire
.26.3 Key Board (for automated model)
1. Basic Functions
Key testing.
2. Principle
79
J1: Key Testing Input;

.26.4 Reflective Optocoupler Adapter Board (for automated model)
1. Basic Functions
Detects spindle in position signals of rotating scanning mechanism.
2. Principle
80
J1: Reflective Optocoupler Detection Input;

3 OUT Optocoupler detects in position signals
.27 Wire Interconnection Diagram
81
82
83
Part No. Name Connection Point

RT861.85.07 Electric Cable for Main Control Power Module-Main Control Board
1-5-082-014-10
Board J14
1-5-082-015-10 RT861.85.08 Electric Cable for Driver Board Power Module-Driver Board J36
RT861.85.11 Electric Cable for Burning Power Module-Burning Board J5
1-5-082-017-10
Board
RBC Bath Electrode Plate-Burning
1-5-082-018-10 RT861.85.14 RBC Signal Receiving Cable
Board J4
WBC Bath Electrode Plate-Burning
1-5-082-019-10 RT861.85.15 WBC Signal Receiving Cable
Board J2
M a i n C o n t r o l B o a r d
1-5-082-020-10 RT861.85.16 HGB Connection Cable
Photocell
Main Control Board J12-Burnin
1-5-082-021-10 RT861.85.17 RBC Signal Switch Cable
Board J3
Main Control Board J11-Burnin
1-5-082-022-10 RT861.85.18 WBC Signal Switch Cable
Board J1
N e t w o r k S w i t
1-5-082-024-10 RT861.85.20 Network Switch Cable
Control Board P5
LED Board J1-Main Control Board
1-5-082-028-10 RT861.85.24 Indicator Cable
J18
1-5-082-034-10 RT861.85.30 Laser Head Cable Laser-Laser Driver Board LASER
Waste Syringe Motor WA- Driver
1-5-082-042-10 RT861.85.38 Waste Syringe Cable
Board J6
Sampling Vertical Motor-Sampling
1-5-082-043-10 RT861.85.39 Vertical Motor Cable
Assy Connector Board J3
RT861.85.41 Sampling Vertical Optocoupler Vertical Optocoupler
for Sampling
1-5-082-045-10
Cable Assy -Connector Board J1
RT861.85.54 Temperature Sensor Adapter Room Temperature Sensor a
1-5-082-053-10
Cable Driver Board J39
RT861.85.56 Waste Liquid Level Sensor Waste Liquid Sensor (Externa
1-5-082-055-10
(External) Waste Liquid Sensor (Internal)
1-5-082-071-10 RT861.85.76 Standard Network Cable PC-Network Port Switch Board
Laser Driver Board LASER J3-LED
1-5-082-072-10 RT861.85.77 Laser Indicator Docking Cable
Board
R T 8 6 1 . 8 5 . 0Ca9 b El f elo eA
r c ut tr oi cm Aau t to m
i act i c S a m p l iD
n rgi v e r B o a r d
1-5-082-016-10
Sampling J8-Power Module
RT861.85.19 Main Control Board-Burning Board M a i n C o n t r o l B o a r d J 2 2 - B u r n i n
1-5-082-023-10
Connection Cable Board J6
R T 8 6 1 . 8 5 . 2 1f o SEr en rc i l aM
o l sa ieCn da C
b ol en t r o l B o a r d
1-5-082-025-10
Machine Board J11
M a i n C o n t r o l B o a r dB aJ r2c0o- d e
1-5-082-026-10 RT861.85.22 Barcode Scan Connection Cable
Scanner
84
M a i n C o n t r o l B o a r d J 1 7
1-5-082-027-10 RT861.85.23 Door Open/Close Cable
Suction and Door Microswitches
Main Control Board J18-Key Board
1-5-082-029-10 RT861.85.25 Key Board Connection Cable
and LED Board
M a i n C o n t r o l BFooar rwda rJ d7 -
R T 8 6 1 . 8 5 . 2 6 F o r w a r d S m a l l - a n g l e
1-5-082-030-10 S m a Pl rl e- aa nm gp
Cable
Board FS
Main C o n t r o l BFooar rwda rJ d8 -
R T 8 6 1 . 8 5 . 2 7 F o r w a r d L a r g e - a n g l e S
1-5-082-031-10 L a r gP er -e aa nm g p
Cable
Board FL
R T 8 6 1 . 8 5 . 2 8 S i d e S c a t Mt ea r ii nn g C
A no g nl et rSSoii gld ne aB l o a
1-5-082-032-10
Cable Preamplification Board SS
M a i n C o n t r o l L B
a so ea r d J 1
1-5-082-033-10 RT861.85.29 Laser Driver Cable
Driver Board J1 and Laser Lock
R T 8 6 1 .H 8o 5r .i 3z M1o on t t Coa orl n n e Hc ot ri i oz nM
o no tt afo olr S
r a m p l i n g
1-5-082-035-10
Cable Assy-Driver Board J7
L y s e S y r i n Lg Ye - DM r oi tv oe r r
1-5-082-036-10 RT861.85.32 Lyse Syringe Cable
Board J3
Sh e at h F l uid Sy r in gS
e HM- o t o r
1-5-082-037-10 RT861.85.33 Sheath Fluid Syringe Cable
Driver Board J2
D i l u e n t S y r i n gD eI - M
D ro it vo er r
1-5-082-038-10 RT861.85.34 Diluent Syringe Cable
Board J1
S a m p S
l i yn r gi n g e A MS oP t - o r
1-5-082-039-10 RT861.85.35 Sample Suction Syringe Cable
Driver Board J4
S a m p l e S y r i nMgoet o rS P - D r i v e r
1-5-082-040-10 RT861.85.36 Sample Syringe Cable
Board J5
D r i v eB ro a r Jd 8 - S a m p l i n g Assy
1-5-082-041-10 RT861.85.37 Vertical Motor Adapter Cable
Adapter Board J3
D r i v eB
r o a r dJ 1 - S a m p l i n g A s s y
1-5-082-044-10 RT861.85.40 Vertical Position Sensor Cable
Adapter Board J13
Driver Board J10-Position Sensors
1-5-082-046-10 RT861.85.42 Position Sensor Connection Cable (DI\SH\SA(SP)\WA\HE(LY),
AS(ASP)\HO (Horizontal))
D r i B
v eo ra Jr 2d 9 - D I F F B
1-5-082-047-10 RT861.85.43 Heater Cable
Heating Film
1-5-082-048-10 RT861.85.44 Pump Connection Cable Driver Board J16-Pump
D r Bi vo Jea R 3rr o 9d o- m
1-5-082-049-10 RT861.85.45 Room Temperature Sensor Cable
Temperature Sensor
S a m p l i n g A s s y C o n nB e co taorrd
R T 8 6 1 . 8 5 . 4 6 S a m p l i n g A s s y T r a n s m i s s i
1-5-082-050-10 J 4 -S a m p l i n g A s s y A d a pBt eora r d
Cable (Enclosed)
J2
1-5-082-051-10 RT861.85.47 Valves V1-V13 Driver Board J37-Valves V1-V13
1-5-082-052-10 RT861.85.48 Valves V21-V32 Driver Board J38-Valves V21-V32
85
RT861.85.57 Waste Liquid Level Sensor CableD r i v Be o

r a rJd2 W
1 -a s t e L i q u i d
1-5-082-056-10
(Internal) Sensor Cable (Internal)
R T 8 6 1 . 8 5 . 5 8 D i f f B a t h D p e rr a t Bui r e o
Te m a s o1rr
v S eJ ne 7d -
1-5-082-057-10
Cable Temperature Sensor
A u t o l o a d eB r l eb no da er rd
1-5-082-059-10 RT861.85.62 Blender Circle Motor Cable
Circle Motor
A u t o l o a d e r b o
P au rs dh bJ a1 c5 k-
1-5-082-060-10 RT861.85.63 Pushback Motor Cable
Motor
A u t o l o a d e r b o a rPdr oJp1u2l -s i o n
1-5-082-061-10 RT861.85.64 Propulsion Motor Cable
Motor
A u t o l o a d eB r l eb no da er rd
1-5-082-063-10 RT861.85.66 Blender Horizontal Motor Cable
Horizontal Motor
A u t o l o a dB el er n bd oe
1-5-082-064-10 RT861.85.67 Blender Vertical Motor Cable
Vertical Motor
R T 8 6 1 . 8 5 . 6 8 A u t o Amu tao l ot ai d ec r b oSa rad m
J 3 -pS w
l i it cnh egs o rS
1-5-082-065-10
Connection Cable 2 Optocouplers
R T 8 6 1 . 8 5 . 6 9 A u t o Autoloader
m a t i board
c J5-Optocouplers
S a m p l i n g S
1-5-082-066-10
Connection Cable 1
A u t o l o a
1-5-082-067-10 RT861.85.71 Electromagnet Connection Cable
Electromagnet
R T 8 6 1 . 8 5 . 7 3 A u t o m a t i c S a m p l i nAutoloader
g S e r i aboard
l P o J6-Main
rt Control
1-5-082-068-10
Connection Cable Board J4 and Driver Board J11
R T 8 6 1 . 8 5 . Ca
7 4b l fEeol re cl Eot d
nrs ci A
ec u t o l o aP d o e w r e rb
1-5-082-069-10
Sampling Assy Module
86
Chapter 4 Software System
.28 Overall Software Architecture

The overall software architecture is divided into three layers: i.e. Information Process
System, main board application, and parts control software. The parts control software is mainly
responsible for drive control of mechanical components, and has the functions of temperature
and pressure testing. The main board application realizes storage of instrument configuration
parameters, execution of control commands sent by the Information
parsing of sequential action, collection of signal data, etc. The Information Processing System is
responsible for interaction with users, operating and controlling instrument running, displaying
and pr i nt i n g r e su l ts, e tc. As o n l y t h e I n f o rma t i on P ro ce ssi n g S yste m i n te r a cts wi t h user s, t he
main board application and parts control software will not be described.
The overall software architecture is shown in the figure below:
Figure 1-1 Overall Software Architecture Chart
87
.29 Functions of Information Processing System Software
Figure 1-2 Functional Chart of Analysis Software

Main function modules:
Module Functional Description
Sample Analysis Sets the information on the next sample, starts test analysis,
d i s p l a y s s, r es sc ua lt tt esr g
a rn ad m h i s t so g
o rf a amn a l y s i s
items, gives alarms or prompts of parameter results,
clinical pro f omrap bt sn o r m a l s a m p l e s , a n d d i s p l a y s s t u d
parameters. The analysis results aresaved in the history sheet
automatically.
Worklist T h e W o r k l i s t i s m a i n l y f ou rsi en dp u ot f information of work
orders of samples to be analyzed before sample ana
r e a idn g o if n f o r m a t i o n o n s a m p l e s t o d b ef r a
onma lt yh ze e
W o r k i l ni s st e q u e n c e a n d , w h e n t h e t e s t
s t ao gr e i o n f f o r m a t i o n o f t h e W o r k l i s t
s h e . e tI n f o r m a t i o n o n s a ma pn lael sy zt eo d b ae n d b e i n g
analyzed can be edited in the Worklist ; information on samples
88
analyzed is edited on the Chart Review interface.

List Review F o r s a m p l e s b r o w s eadn aal ny dz e d i n t h e f o r m o f l i s t , t h i s
mod ule ca n make sta tistics o f th e re sul CVtinva
g lue s, export
r e s u l t s t o E x c e l t a b l e , a n d c o n d u c t v e r
deletion, printing, etc.
Chart Review Displays sample information in the forms of chart and sample
details and can conduct editing, verification, d
printing of sample results and data. The open model can start
sample testing on this interface.
Quality Control L - uJ a Conducts
lQ i quality
t control
y of the instrument with
Control QC. L-J Quality Control has Whole Blood and
Pre-diluted modes and allows you to conduct
quality control of all or certain parameters.
X - B
u a XQ-l B iu
Qa lt i tCo
yy n t r od lo e s n o t u s e Q C a n d
Control makes statistical analysis according to the
samples tested. The analyzer conducts X-B
Quality Control of three parameters, i.e. MCV,
MCH, and MCHC, and whole blood and pre-
d i l u t e d s a m p l e s c a n b e u s e d a s s a
sources.
X - R
u a Ql i Qut ay l i Co t y n t , r oi .l e . M e a n - R a n g e
Control Qu a l i tCo
y n t r, o il s a n e f f e c t i v e m e t h o d f o r
judging and forecasting whether the quality
status fluctuates abnormally. This method and
L-J Quality Control complement each other. X-
R conducts quality control analysis with QC.
Calibration Automatic According to your settings, the instru
sucks the calibration material or fresh human
blood to calculate the calibration coefficient
automatically.
Manual Y o u c h a n g e t h e c a l i b r a
manually.
Daily Maintenance Provides the functions for instrument maintenance to make the
instrument work steadily and normally.
Services Provides auxiliary system functions, monitors the instrum
status, debugs the instrument, checks system information, etc.
System Setup Sets the changeable items relating to daily opera
instrument maintenance.
Power-on The power-on process completes assy initialization, instrument
c h e c k , c l e a n i n g , a n d b a c k g r o u n d t e s
sequence, cleaning time and action depend on the situation
when the machine is powered off last time.
Exit C a n c o n d u c t 1 . M i n i m i z Pa ot iwo en r , o2 f . f L o g o
Instrument, 4. Exit System, etc.
89
I n w h i c h P , of w
o re r “ I on fsf t r u m e n t ” , p o n w leyr - t oh f ef
procedure is executed, and you will not exit the Information
Processing System analysis software . For “Exit System”, after
t h e s a m e o p e r a t i o n aP so wt he ar t Ionff o
sf tr r u
“ ment” is
executed, the system will prompt whether to exit the Information
Processing System software.
90
Chapter 5 Fluidics System
.30 Functions of Fluidics Devices

.30.1 Valves
Material Icon Name Purpose

To s w i t c h b e t w e e n d i l u e n t s u c t i o n c o n t r o l o r d i l u t e d
Three-way Valve SV1
sample suction from DIFF bath
To switch between diluent suction control or sampling
Three-way Valve SV2
probe inner wall cleaning control
To switch between diluent suction control or sampling
Three-way Valve SV3
probe outer wall cleaning control
To switch between sheath flow channel or sample flow
Two-way Valve SV4
channel
Two-way Valve SV5 Sampling probe inner wall cleaning control
Two-way Valve SV6 Flow Cell sheath flow control
Three-way Valve SV7 To switch between Flow Cell or WBC and RBC baths
To switch b etwe en cle an se r su cti on co ntro l or WBC
Three-way Valve SV8
and RBC baths
Three-way Valve SV9 To switch between WBC or RBC bath liquid adding
Three-way Valve SV10 86D lyse control
Three-way Valve SV11 DIFF bath constant temperature diluent control
Three-way Valve SV12 86H lyse control
Three-way Valve SV13 Diluent control
Ba c k ba t h d i l u e n t s u c t i o n c o notfrW
o lB C a n d R B C
Two-way Valve SV21
baths
Two-way Valve SV22 Waste Syringe drain control
Two-way Valve SV23 Waste Syringe air control
Two-way Valve SV24 WBC and RBC/PLT testing control
Two-way Valve SV25 Flow Cell outlet drain control
Two-way Valve SV26 RBC bath drain control
Two-way Valve SV27 WBC bath drain control
Two-way Valve SV28 DIFF bath drain control
Pinch Valve SV29 DIFF bath sample preparation control
Two-way Valve SV30 DIFF bath, WBC bath, and RBC bath bubble control
S a m p l i n g p r o b e i n n e r a n d o u t e r w a
Two-way Valve SV31
control
D I F F b a t hW, B C b a t h , a n d R B C b a t h m a s t e r d r a i n
Two-way Valve SV32
control
.30.2 Syringes
91

Sampling Syringe ASP Sample suction and distribution
W B C 5 - p adr it f f e r e n t i a t i o n t e s t i n g p r o c e s s s a m p l e
Sampler SP
quantification
86D Diluent Syringe 86D DIL To spit diluent to DIFF bath
86D Lyse Syringe 86D LY To add 86D lyse to DIFF bath
86H Lyse Syringe 86H LY To add 86H lyse to WBC bath
C o u n t i n g c h a m b e r l i q u i d a d d i n
Sheath Fluid Syringe SH sheath fluid formation in Flow Cell, cleaning of Flow
Cell and counting chamber related pipes
Diluent Syringe DIL To clean sampling probe and Flow Cell related pipes
Waste Syringe WA Pressure building and storage
.30.3 Other Auxiliary Devices

To drain waste liquid (including Rinse part, DIFF bath,
Waste Liquid Pump P1
WBC and RBC baths)
Rinse part Rinse part To clean inner and outer walls of sample probe
To p r e v e n t b u b b l e a n d w a s t e l i q u i d r e f l u x ; t o b l o c k
Buffer Bath Buffer Bath
outside interference signals
Air Pressure
Air Pressure Sensor To monitor internal pressure of Waste Syringe
Sensor APS
Liquid Pressure Liquid Pressure
To monitor pressure of sheath flow pipes
Sensor Sensor HPS
*** Liquid Level
Liquid Level Sensor To detect whether there is any reagent or not
Sensor
92
.31 Whole Machine Test Process

.31.1 Flowchart of CBC+DIFF Test in Whole Blood Mode
Sampling probe
sucks blood
sample 20μL
Diluent Diluent
615μL 2008.9μL
DIFF Bath Blood WBC Bath Blood Discard redundant

Distribution 10μL Distribution 6μL blood sample to
Rinse part
5D Lyse 375μL
Sampling probe sucks

DIFF Bath WBC Bath initial diluent
Constant Temp. Blending (dilution Blending (dilution
ratio 1:100) ratio 1:335)
5H Lyse 200μL Diluent 2630μL
Enter Flow Chamger WBC Bath RBC Bath Blending

WBC 5-part Differentiation Secondary Blending (dilution ratio
Measurement (dilution ratio 1:370) 1:18000)
Flow Chamger WBC Test RBC and PLT Tests

Cleaning Sampling probe
HGB Measurement
sucks blood
sample 20μL
Diluent WBC Bath Diluent

RBC Bath
615μL Cleaning/Burning 2008.9μL
Cleaning/Burning
DIFF Bath Blood WBC Bath Blood Discard redundant

Distribution 10μL Distribution 6μL blood sample to
Rinse part
End of Test
5D Lyse 375μL
Sampling probe sucks

.31.2 Process of CBC Test in Whole Blood Mode initial diluent
DIFF Bath WBC Bath
Except the DIFF bath channel that does not
Constant Temp. Blending (dilution Blending
participate (dilution
in the
the testing process for test,
ratio 1:100) ratio 1:335)
WBC and RBC baths is the same as the above figure.
.31.3 Flow chart of CBC+DIFF Test in Pre-diluted Mode 5H Lyse 200μL Diluent 2630μL
Enter Flow Chamger WBC Bath RBC Bath Blending

WBC 5-part Differentiation Secondary Blending (dilution ratio
Measurement (dilution ratio 1:370) 1:18000)
Flow Chamger WBC Test RBC and PLT Tests

Cleaning HGB Measurement
WBC Bath RBC Bath

Cleaning/Burning Cleaning/Burning
93
End of Test
.31.4 Process of CBC Test in Pre-diluted Mode

Except the DIFF bath channel that does not participate in the test, the testing process for
WBC and RBC baths is the same as the above figure.
.32 CBC+DIFF Measurement Sequence Description

.32.1 DIFF Bath Sample Distribution and Blending after 86D Lyse Adding
(0~14S)
1. Draining DIFF bath base solution: Power on and open the SV28 and SV32 valves,
and the liquid pump P1 drains the base solution from the DIFF bath.
2. Spitting diluent, distributing blood sample, and adding 86D lyse to DIFF bath: Power
94
Enter Flow Chamger
DIFF Bath WBC Bath
WBCBath
WBC Test
Bath RBC Bath Blending
WBC 5-part
BlendingDifferentiation
(dilution WBC
Flow Chamger Cleaning/Burning
HGB
SecondaryMeasurement
Blending RBC Bath
(dilution ratio
Constant Temp Measurement
ratio 1:250) Blending (dilution
End of Test Sampling probe sucks initial diluent 2668.7μL
Cleaning 5H Lyse 200μL RBC and PLT
Cleaning/BurningTests
Diluent
(dilution ratio 1:750)
ratio 1:698) 1:24000)
on and open the SV11 valve, and the 86D DIL syringe spits diluent to the DIFF bath. The
sampling probe moves to the DIFF bath, and the ASP syringe distributes 10ul blood
sample from the sampling probe to the DIFF bath. At the same time, power on and open
the SV10 valve, and the 86D LY syringe spits lyse to the DIFF bath.
3. DIFF bath sample bubble blending: Power on and open the SV30 and SV28 valves,
and the Waste Syringe establishes positive pressure (5.5~6.5Kpa) and adds bubble to
the DIFF bath.
Schematic Diagram of DIFF Bath Drain
95
Schematic Diagram of Spitting Diluent, Distributing Blood Sample and Spitting Lyse to DIFF
Bath
96
Schematic Diagram of DIFF Bath Bubble Blending

.32.2 DIFF Channel Test (19~40S)
1. Sample preparation: Power on and open the SV1 valve, and the DIL syringe sucks
the diluted and blended sample from the DIFF bath. After the suction, the pinch valve
SV29 (pipe stop valve) is powered on, and the sample is stored in the pipe.
2. Sheath flow formation: Power on and open the SV13, SV6, and SV25 valves, and
the SH syringe spits diluent to the Flow Cell at a certain speed to form a stable sheath
flow.
3. Rapid sample pushing: Power on and open the SV4 valve, and the sample is rapidly
pushed to the Flow Cell with the bypassing effect of the sample pushing of the SH
syringe. At the same time, before the SV4 valve is closed, the SP syringe pushes the
sample to the test area of the Flow Cell at a high speed.
4. DIFF channel test: After the sample reaches the test area of the Flow Cell, the SP
syringe pushes the sample to the Flow Cell at a low speed. The sample, wrapped by the
sheath flow, passes through the test area of the Flow Cell steadily, and the test begins
97
14S.
Schematic Diagram of Sample Preparation
98
Schematic Diagram of Sheath Flow Formation
99
Schematic Diagram of Rapid Sample Pushing
100
Schematic Diagram of DIFF Channel Test

.32.3 DIFF Channel Cleaning (40～53S)
1 Cleaning Flow Cell: When the test is finished, the SV25 valve is kept on and opened
for 2S for cleaning the Flow Cell and the Flow Cell outlet related pipes.
2 Back flushing sample probe in Flow Cell: Power off and close SV25 and power off
SV29 (the pipe is through), and the SH syringe pushes the diluent to back flush the
sample probe in the Flow Cell. At the same time, power on and open the SV1 valve, and
the DIL syringe pushes the diluent to clean the sample preparation pipe.
3 Cleaning sample preparation pipe: Power on and open the SV1 valve, and the DIL
syringe pushes the diluent to clean the sample preparation pipe.
4 Adding base solution to DIFF bath: After the DIFF bath is drained, power on and
open the SV1 valve, and the DIL syringe pushes 1.2ml diluent to the DIFF bath to soak
the DIFF bath.
101
Schematic Diagram of Cleaning Flow Cell
102
Schematic Diagram of Back Flushing Sample Probe in Flow Cell
103
Schematic Diagram of Cleaning Sample Preparation Pipe

.32.4 HGB Background Measurement, WBC Bath and RBC Bath Drain, and
Diluent Spitting (0.5~15S)
1. HGB background measurement: 0.5S HGB background measurement
2. Draining RBC bath: Power on the SV26 and SV32 valves, and drain the RBC bath
with the waste liquid pump.
3. Draining WBC bath: Power on the SV27 and SV32 valves, and drain the WBC bath
with the waste liquid pump.
4. Spitting diluent to RBC and WBC baths: Power on and open the SV13 and SV7
valves, and the SH syringe will spit diluent to the RBC bath. Open the SV9 valve, and
diluent will be spit to the WBC bath.
104
Schematic Diagram of Draining WBC and RBC Baths
105
Schematic Diagram of Spitting Diluent to WBC and RBC Baths

.32.5 WBC Bath Sample Distribution and Initial Mixture Blending (15~17.6S)
1. Distributing blood sample to WBC bath: The sampling probe moves to the WBC
bath position, and the ASP syringe distributes 6ul blood sample to the WBC bath.
2. Initial mixture blending in WBC bath: Power on and open the SV30 and SV27
valves, and the WA syringe will establish positive pressure (5.5~6.5Kpa) and add bubble
to the WBC bath.
106
Schematic Diagram of Initial Mixture Blending in WBC Bath

.32.6 Initial Diluted Mixture Suction (19.6~21.6S)
The sampling probe sucks the initial diluted mixture from the WBC bath.
.32.7 Secondary Blending after 86H Lyse Spitting to WBC (21~25S)
1. Spitting 86H lyse to WBC bath: Power on and open the SV12 valve, and the 86H LY
syringe will spit 0.2ml lyse to the WBC bath.
2. Secondary blending in WBC bath: Power on and open the SV30 and SV27 valves,
and the WA syringe will establish positive pressure (4.5~5.5Kpa) and add bubble to the
WBC bath.
107
Schematic Diagram of Secondary Blending in WBC Bath

.32.8 RBC Bath Sample Distribution and Blending (25~27S)
1. Distributing initial diluted mixture to RBC bath: The sampling probe moves to the
RBC bath position, and the ASP syringe spits the initial diluted mixture sucked from the
WBC bath to the RBC bath.
2. Blending in RBC bath: Power on and open the SV30 and SV26 valves, and the WA
syringe will establish positive pressure (4.5~5.5Kpa) and add bubble to the RBC bath.
108
Schematic Diagram of Blood Adding and Blending in RBC Bath

.32.9 WBC and RBC Test, and HGB Sample Measurement (27~46S)
1. Establishing negative pressure for testing: The WA syringe establishes negative
pressure (-23~-24Kpa).
2. Flushing back bath before testing: Power on and open the SV24 and SV21 valves,
and the testing pipes of the back baths of the WBC and RBC baths will be flushed.
3. WBC, RBC/PLT test: Power off and close the SV21 valve, and the SV24 valve is
kept on and opened. The CBC channel test begins after the liquid flow becomes stable
12S.
4. HGB test: 45S, HBG sample value measurement.
109
Schematic Diagram of Flushing Back Bath before Testing
110
Schematic Diagram of CBC Channel test

.32.10 WBC and RBC Baths Cleaning (46~60S)
1. Initial cleaning of WBC bath: Power on and open the SV27 and SV32 valves, and
drain the liquid from the front bath of the WBC bath with the waste liquid pump. Power
on and open the SV13, SV7, and SV9 valves, and the SH syringe will spit 3.5ml diluent
to the WBC bath to clean the WBC bath.
2. Adding RBC base solution: Power on and open the SV26 and SV32 valves, and
drain the liquid from the front bath of the RBC bath. Power on and open the SV13 and
SV7 valves, and the SH syringe will spit 3.5ml diluent to the RBC bath to soak the RBC
bath.
3. Adding WBC base solution: Power on and open the SV13, SV7, and SV9 valves,
and the SH syringe will spit 2.5ml diluent to the WBC bath to soak the WBC bath.
4. Flushing back bath pipe: The WA syringe establishes negative pressure (-30~-
35Kpa). Power on and open the SV21 and SV24 valves and, after 2.5S, flush the testing
pipe of the back bath.
111
5. Burning Ruby Aperture: Start high pressure to burn the Ruby Aperture for 4S.
.33 Functions of Fluidics Sequence

.33.1 Test Sequence
All measurements include: measurements of WBC, RBC, PLT and HGB and related
parameters:
1. DIFF+CBC test in Whole Blood mode: WBC 5-part differentiation test.
2. CBC test in Whole Blood mode: WBC 3-part differentiation test.
3. DIFF+CBC test in Pre-diluted mode: Peripheral blood 5-part differentiation test.
4. CBC test in Pre-diluted mode: Peripheral blood 3-part differentiation test.
.33.2 Replacement of Reagents

1. Diluent: The diluent is replaced independently.
2. Cleanser: The cleanser is replaced independently.
3. 86H lyse: The 86H lyse is replaced independently.
4. 86D lyse: The 86D lyse is replaced independently.
.33.3 Clean
1. Clean WBC bath: The front and back baths of the WBC counting chamber are
cleaned, in which, the front bath is cleaned with the initial bubble.
2. Clean RBC bath: The front and back baths of the RBC counting chamber are
cleaned, in which, the front bath is cleaned with the initial bubble.
3. Clean DIFF bath: The DIFF bath is cleaned with the initial bubble.
4. Fluidics Clean: The Fluidics cleaning is executed to clean all testing pipes and
counting chambers.
5. Clean probe: The inner and outer walls of the sampling probe module are cleaned,
after which, isolation bubble is established.
6. Clean Flow Cell: The DIFF channel testing pipes is cleaned, including cleaning of
pipes for sample preparation, sample pushing, sheath flow, Flow Cell, etc.
.33.4 Upkeep
1. Unclog: The Ruby Aperture of the WBC/RBC counting chamber is zaped; positive
pressure is established to back flush the Ruby Aperture of the WBC/RBC counting
chamber, after which, the front and back baths of the WBC/RBC counting chamber are
cleaned.
2. Zap Apertures: The Ruby Aperture of the WBC/RBC counting chamber is zaped for
112
8S.
3. Back Flush: Positive pressure is established to back flush the Ruby Aperture of the
WBC/RBC counting chamber.
4. DIFF bath soak: The DIL syringe sucks 1.5ml concentrated cleanser through the
sampling probe. The DIFF testing channel related pipes (including pipes for sample
preparation, sample pushing, sheath flow, Flow Cell, etc.) are soaked for 5 minutes with
the mixture automatically diluted with the diluent spit at the ratio of 1:3 in the machine.
The pipes soaked with the cleanser are fully flushed with the diluent.
5. WBC/RBC bath soak: The DIL syringe sucks 1.8ml concentrated cleanser through
the sampling probe. The front and back baths of the WBC and RBC counting chambers
are soaked for 5 minutes with the mixture automatically diluted with the diluent spit at the
ratio of 1:3 in the machine. The front and back baths of the WBC and RBC counting
chambers soaked are rinsed with the diluent.
6. Fluidics conc. Cleanser soak: The DIL syringe sucks 3.3ml concentrated cleanser
through the sampling probe. The DIFF testing channel related pipes and the front and
back baths of the WBC and RBC counting chambers are soaked for 5 minutes with the
mixture automatically diluted with the diluent spit at the ratio of 1:3 in the machine. The
pipes of the whole machine soaked are rinsed with the diluent.
7. Cleanser soak: The cleanser is sucked to soak the DIFF testing channel related
pipes and the front and back baths of the WBC and RBC counting chambers for 10
minutes (adjustable). The pipes of the whole machine soaked are rinsed with the diluent.
8. Flow cell soak.
.33.5 Fluidics Maintenance

1. Empty WBC bath: The liquid in the WBC bath is emptied independently.
2. Empty RBC bath: The liquid in the RBC bath is emptied independently.
3. Empty DIFF bath: The liquid in the DIFF bath is emptied independently.
4. Empty Fluidics: The liquid in all pipes of the analyzer is emptied.
5. Initialize: The syringes and sampling probes are initialized.
6. Pack: The instrument needs to be packed before long-distance transport or it is out
of service for more than 3 days. Firstly, empty the liquid in all pipes; then perfuse distilled
water into all pipes; and then drain the liquid in the pipes again.
113
7. Prime: All pipes of the analyzer are primed, including perfusion of diluent, cleanser,
86H lyse, and 86D lyse.
.33.6 Functions of Other Sequences

1. Spitting Diluent in Pre-diluted Mode
Function: Spitting 180μl diluent for peripheral blood dilution and quantification.
2. Power-on after Normal Power-off
Function: After daily leak detection of related pipes and pipe cleaning upon normal
power-on of the analyzer, the background test is executed once. If the testing result does not
meet the background range, the background is tested once again.
3. Power-on after Abnormal Power-off
Function: After the leak detection of related pipes and pipe cleaning upon power-on after
the last abnormal power-off of the analyzer (the cleaning intensity is higher than that for
normal power-on), the background test is executed once. If the testing result does not meet
the background range, the background is tested once again.
4. Power-on after Drain and Power-off
Function: After the leak detection, automatic perfusion and automatic cleaning of pipes
upon power-on after the analyzer is drained, the background test is executed once. If the
testing result does not meet the background range, the background is tested once again.
5. Power-off Maintenance
Function: Daily power-off maintenance. The pipes of the counting chamber and DIFF
channel testing pipes of the analyzer are soaked with the cleanser. The pipes of the Fluidics
soaked are rinsed.
6. Sleep
Function: The laser is turned off; the WBC/RBC bath is topped up with reagent for
soaking; the instrument is in the sleep mode (is standby).
7. Quit Sleep 1
Function: Used when the sleep time of the instrument is less than 1 hour. The counting
chamber and Flow Cell related pipes are cleaned.
8. Quit Sleep 2
Function: Used when the sleep time of the instrument is greater than 1 hour and less
than 3 hours. The counting chamber and Flow Cell related pipes are cleaned.
9. Quit Sleep 3
Function: Used when the sleep time of the instrument is greater than 3 hours. The
counting chamber and Flow Cell related pipes are cleaned.
10. Quit Sleep 4
Function: Used when the sleep time of the instrument is greater than 6 hours. The
counting chamber and Flow Cell related pipes are cleaned.
11. Negative Pressure Self-test
Function: Pressure self-test function of the Waste Syringe.
114
12. Positive Pressure Self-test
Function: Pressure self-test function of the Waste Syringe.

13. DIFF Channel Pipe Leak Detection
Function: The sequence is executed during power-on to conduct leak detection of DIFF
testing channel pipes. The detection positions include sheath flow pipe, sample pipe, and
Flow Cell.
14. WBC/RBC Channel Pipe Leak Detection
Function: The sequence is executed during power-on to conduct leak detection of CBC
channel pipes. The detection positions include drain pipe and CBC testing pipe.
115
Chapter 6 Mechanical System
.34 Structure of Instrument

.34.1 Structure of Major Boards of Instrument
Structure of Instrument (Front of instrument)
1 ---- Decorative Screen 2 ---- Open Sample compartment door Key

3 ---- Test Key 4 ---- Sample compartment Door
116
Structure of Instrument (Back of instrument)
1 --- Fuse Holder 2 --- Filter

3 --- 86D Lyse Connector 4 --- 86H Lyse Connector
5 --- Diluent Connector 6 --- Cleanser Connector
7 --- Waste Liquid Connector 8 --- Waste Liquid Sensor Connector
9 --- Network Port Connector 10 --- Earthing Rod
117
Structure of Instrument (Front door opened)
1 --- Rotating Code Scan Assy 2 --- Blending Assy

3 --- Enclosed Sampling Assy 4 --- Sampling Syringe
5 --- Sample Syringe 6 --- Diluent Syringe
7 --- Valve 2 8 --- Valve 1
9 --- Valve 4 10 --- Valve 3
11 --- Valve 5 12 --- Sampling Assy
13 --- Sampling Probe
118
Structure of Instrument (Right door opened)
1 --- Laser Power Lock 2 --- Sampling Assy

3 --- Pinch Valve 29 4 --- Valve 25
5 --- Filter 6 --- Valve 30
7 --- Valve 26 8 --- Valve 31
9 --- Valve 27 10 --- Waste Liquid Pump Assy
11 --- Valve 32 12 --- Valve 28
13 --- Counting Chamber Assy 14 --- Buffer Bath Assy
15 --- DIFF Bath Assy 16 --- Automatic Sampling Assy
17 --- Optical Assy
119
Structure of Instrument (Left door opened)
1 --- Liquid Pressure Sensor 2 --- Valve 6

3 --- Valve 7 4 --- Pinch Valve 8
5 --- Valve 9 6 --- Valve 13
7 --- Valve 12 8 --- Valve 11
9 --- Valve 10 10 --- Sheath Fluid Syringe
11 --- Lyse Syringe 12 --- Valve 22
13 --- Valve 24 14 --- Valve 23
15 --- Valve 21 16 --- Waste Syringe
17 --- Power Supply Switch 18 --- Power Supply Assy
.35 Dismantling of Instrument

This section mainly describes the methods and steps of replacing the assys.
.35.1 Opening Left and Right Doors
As shown in the figure, open the locks for the left and right doors with keys, and open
the left and right doors along the rear hinge.
120
.35.2 Opening and Closing Top Shell

1. Opening
As shown in the figure, open the left and right doors and screw out the screws (at total 2
positions) indicated by the arrow with a cross screwdriver, and the top shell can be flipped
121
upward along the hinge.
122
2. Closing
Reverse the steps of opening the top shell to close it.
.35.3 Dismantling of Shell of Automatic Sampler
1. Removal
As shown in the figure, open the left and right doors and screw out the screws (at total 4
positions) indicated by the arrow with a cross screwdriver.
123
2. Mounting
Reverse the steps of removal to mount it.
.35.4 Dismantling of Automatic Sampling Driver Board
124
1. Removal of Launch Tray

As shown in the figure, after the work described in Sections 6.2.1 and 6.2.3 is finished,
screw out the screws (at total 4 positions) indicated by the arrow with a cross screwdriver.
2. Removal of Shield for Autoloader board

As shown in the figure, screw out the screws (at total 2 positions) indicated by the arrow
with a cross screwdriver.
125
3. Removal of Autoloader board

4. Mounting
.35.5 Dismantling of Bottom Shell

1. Removal
As shown in the figure, open the left and right doors and the shell of the automatic
126
sampler, and screw out the screws (at total 6 positions) indicated by the arrow with a cross
screwdriver.
127
2. Mounting
.35.6 Dismantling of Top Plate
1. Removal
As shown in the figure, screw out the 4 screws indicated by the arrow with a cross
screwdriver.
128
2. Mounting
.35.7 Dismantling of Rear Door

1. Removal
As shown in the figure, screw out the screws (at total 10 positions) indicated by the
arrow with a cross screwdriver.
2. Mounting
129
.35.8 Dismantling of Cooling Fan, Main Control Board, and Driver Board
1. Removal
Open the rear door, as shown in the figure:
1) To remove the cooling fan, screw out the 4 screws indicated by the arrow with a
cross screwdriver.
2) To remove the main control board, screw out the 6 screws indicated by the arrow
3) To remove the driver board, screw out the 7 screws indicated by the arrow with a
cross screwdriver.
2. Mounting
Reverse the steps of removal to mount them.
.35.9 Dismantling of Burning Board

1. Removal
As shown in the figure, open the right door, screw out the 2 screws indicated by the
arrow with a cross screwdriver and remove the shield of the burning board. Then the burning
board behind it can be seen. Screw out the 4 screws indicated by the arrow with a cross
screwdriver and remove the burning board.
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2. Mounting
.35.10 Dismantling of Sampling Assy Connector Board and Sampling Assy

Adapter Board
1. Removal
As shown in the figure, open the right door.
1) To remove the sampling assy connector board, screw out the screws (at total 4
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2) To remove the sampling assy adapter board, screw out the screws (at total 3
2. Mounting
.35.11 Replacement of Fuse

As shown in the figure, screw out the fuse holder indicated by the arrow with a straight
screwdriver and replace the fuse.
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.35.12 Dismantling of Power Supply Assy

Caution: Turn off the power of the machine before the operations described in this
section.
1. Removal
1) Unplug the electric cable of the machine.
2) Remove the rear plate and unplug the electric cables of the main control board, driver
board, and fan.
3) As shown in the figure, open the left door and top cover, screw out the screws (at total
2 positions) indicated by the arrow with a cross screwdriver, and the power supply assy can
be drawn out in the direction indicated by the arrow.
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2. Mounting
Reverse the above steps to mount the power supply assy.
.35.13 Dismantling of DIFF Bath Assy and Counting Chamber Assy

Caution: Drain the reagent from the corresponding assy and turn off the power of the
machine before the operations described in this section.
1. Removal
Open the right door, as shown in the figure:
1) To remove the DIFF bath assy
1) Unplug the pipes (4 pcs) connected to the bath and the connection cables (2 pcs).
2) Screw out the 4 screws indicated by the arrow with a cross screwdriver.
2) To remove the counting chamber assy
1) Unplug the pipes (7 pcs) connected to the bath.
2) Remove the rear plate, unplug the connection cables (2 pcs) between the reaction
basin and the high voltage switchboard and the connection cable (1 pcs) connected to the
main control board.
3) Screw out the 6 screws indicated by the arrow with a cross screwdriver.
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2. Mounting
Reverse the above steps to mount the DIFF bath assy or counting chamber assy.
.35.14 Dismantling of Sampling Assy

Caution: Drain the reagent from the corresponding assy and turn off the power of
the machine before the operations described in this section.
1. Removal
1) To remove the sampling assy
[1] Unplug the pipes (3 pcs) connected to the assy (be sure to unplug the pipes from the
end connected to the valve or syringe) and the connection cables (4 pcs, including 1
horizontal motor cable, 1 optocoupler cable, and 2 cables on the adapter board).
[2] Open the right door, as shown in the figure, and screw out the 4 screws with the
mark as indicated by the arrow with a cross screwdriver.
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2) To remove the sampling probe

[1] Push the sampling probe assy to the bottom position.
[2] Cut off the 2 cable ties on the sampling probe assy that fix the sampling probe pipe.
[3] Unplug the pipe connected to the upper part of the sampling probe.
[4] Screw out the pipe fixing the sampling probe assy with a cross screwdriver.
[5] Take up the sampling probe assy.
2. Mounting
Reverse the above steps to mount the sampling assy or sampling probe.
Note: When mounting the sampling probe, please note the following:
1) The direction of the side opening of the sampling probe is the same as that of the
intubation pipe of the Rinse part.
2) The pipe(EVA pipe) connected to the sampling probe is directly inserted in the upper end of
sampling probe, with the adapter pipe wrapped outside. The effect of enclosure is actually caused by
the upper end of sampling probe and EVA pipe, not the adapter pipe.
.35.15 Dismantling of Waste Liquid Pump Assy
Caution: Turn off the power of the machine and prevent liquid from touching the skin
or dropping onto the machine before the operations described in this section.
1. Removal
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Open the right door, as shown in the figure, and screw out the 4 screws indicated by the
arrow with a cross screwdriver.
2. Mounting
Reverse the above steps to mount the waste liquid pump assy.
.35.16 Dismantling of Syringe Assys

1. Removal
Open the front shell, as shown in the figure:
1) To remove the sampling syringe, screw out the 4 screws indicated by the arrow with a
cross screwdriver, remove the sampling syringe, and unplug the terminal blocks on its motor
and optocoupler.
2) To remove the sample syringe, screw out the 4 screws indicated by the arrow with a
cross screwdriver, remove the sample syringe, and unplug the terminal blocks on its motor
and optocoupler.
3) To remove the diluent syringe, screw out the 4 screws indicated by the arrow with a
cross screwdriver, remove the diluent syringe, and unplug the terminal blocks on its motor
and optocoupler.
Open the left door, as shown in the figure:
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4) To remove the sheath fluid syringe, screw out the 4 screws indicated by the arrow with a
cross screwdriver, remove the sheath fluid syringe, and unplug the terminal blocks on its
motor and optocoupler.
5) To remove the lyse syringe, screw out the 4 screws indicated by the arrow with a cross
screwdriver, remove the lyse syringe, and unplug the terminal blocks on its motor and
optocoupler.
6) To remove the Waste Syringe, screw out the 4 screws indicated by the arrow with a
cross screwdriver, remove the Waste Syringe, and unplug the terminal blocks on its motor
and optocoupler.
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2. Mounting
Reverse the above steps to mount the syringe assys.
.35.17 Dismantling of Valves

1. Removal
Open the front shell, as shown in the figure, and screw out the 2 screws that lock the
valve as indicated by the arrow with a cross screwdriver. Remove the valve from the front
board, and unplug the valve and the terminal connected to it.
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Open the right door, as shown in the figure, and screw out the 2 screws that lock the
valve as indicated by the arrow with a cross screwdriver.
Open the left door, as shown in the figure, and screw out the 2 screws that lock the
valve as indicated by the arrow with a cross screwdriver.
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2. Mounting
Reverse the above steps to mount the valves.
.35.18 Dismantling of Liquid Pressure Sensor
Open the left door, as shown in the figure, and screw out the 2 screws indicated by
the arrow with a cross screwdriver.
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.35.19 Dismantling of Optical Path Assy

1. Removal
Open the right door and remove the top cover, as shown in the figure, and screw out the
4 nuts indicated by the arrow with a socket spanner.
2. Mounting
Reverse the above steps to mount the optical assy.
.35.20 Dismantling of Board in Optical Assy
Remove the top plate and open the right door, as shown in the figure. Screw out the 8
screws indicated by the arrow with a cross screwdriver and remove the optical path cover.
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Screw out the 5 screws indicated by the arrow with a cross screwdriver and remove the
optical path cartridge, as shown in the figure.
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As shown in the figure:

1) To remove the side scatter preamplification board, screw out the screws (at total 4
positions) indicated by the arrow with an Allen wrench.
2) To remove the forward small-angle preamplification board, screw out the screws (at total
4 positions) indicated by the arrow with an Allen wrench.
3) To remove the laser driver board, screw out the screws (at total 4 positions) indicated
by the arrow with an Allen wrench.
with an Allen wrench and screw out the screws (at total 2 positions) indicated by the arrow
with a cross screwdriver. Remove the side shielding shell, screw out the screws (at total 4
positions) indicated by the arrow with an Allen wrench, and replace the side preamplification
board.
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2. Mounting
Reverse the above steps to mount the boards relating to the optical system.
Adjustment of laser driver board:
To adjust the laser drive current, make a trial first to determine the adjustment
direction, then slowly adjust the variable resistor VR1, and adjust the potential of the
test port LD_CURT to 3.3V. If the adjustment is too fast or the amplitude of
adjustment is too big, the laser may be damaged.
Requirements:
Testing Voltage: Position No. LD_CURT;
Voltage Range: 3.3V±0.05V;
Conversion to Laser Drive Current (mA):
I=LD_CURT/50/0.76 (mA)
The drive current of the laser is about 85mA;
The radiant power of the laser is about 15mW-22mW (test after focusing)
.35.21 Replacement of Flow Cell
.35.21.1 To remove the Flow Cell
1. Unplug the pipe of the Flow Cell. Prevent the reagent in the pipe from touching the
external surface of the Flow Cell.
2. Screw out the 4 screws shown in the figure.
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3. Remove the bolt. Prevent the spring jumping out.
4. Remove the Flow Cell adjusting plate.
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.35.21.2 Replace with a new Flow Cell and conduct the position test by following the steps
described below
1. Adjust the position of the Flow Cell to make the light spot reflected by the Flow Cell
deviate from the slit for about 2mm, and tighten the screws.
Making the light spot

reflected by the flow
chamber deviate from the
slit for about 2mm 。
The screw
The screw
2. Adjust the central axis of the Flow Cell into the light spot of the laser, as shown in the
figure below. Loosen the adjusting nut, adjust the bolt, and make the detection area of
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the Flow Cell enter the irradiated region of the laser.
Loosen the
adjusting nut
Adjust the bolt, and make the

detection area of the flow chamber
enter the irradiated region of the laser
3. Observe the scattered light spots on both sides of the forward diaphragm for preliminary
judgment of whether the Flow Cell is adjusted in place.
4. If there
is an
oscilloscope, the Flow cell can be adjusted by capturing the presignal of the standard
particles. When the signal amplitude is a maximum, it indicates that the Flow cell is at the
most reasonable position.
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If there is no oscilloscope, the Flow Cell can be adjusted with method of successive
approximation only by testing the standard particles or the sample.
5. At last, tighten the adjusting nut. Do not rotate the screw any more when tightening the
adjusting nut.
.35.21.3 Optical Gain Debugging
1. Adjustment of Standard Particles

Adopt 7um standard latex particles. Enter the “Service” — “Adjustment” interface, select
“DIFF Gain (Particle)”, and conduct the standard particle test. Requirements:
1) FS: PK.AD adjusted to 386-396
2) FL: PK.AD adjusted to 423-439
3) FS: CV ≤ 7%
4) FL: CV ≤ 7%
Otherwise, adjust the gains of FS and FL till the above requirements are met.
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2. Adjustment of Normal Fresh Blood

In general, the scattergram of standard particles is different from that of human blood,
therefore the optical gain needs to be fine-tuned through the scattergram of normal human
blood.
Select “DIFF Gain (Blood)” and enter the new interface. Prepare at least 3 tubes of normal
fresh human blood and conduct the test. The indicators should meet the requirements listed
in the table below.
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Table 6-1 Centre-of-gravity Position of Particle Swarms in DIFF Scattergram
Parameter Target Value Tolerance Lower Limit Upper Limit

NE-FS-P 644 ±32 612 676
LY-FS-P 453 ±32 421 485
MO-FS-P 660 ±32 628 692
NE-FL-P 601 ±48 553 649
LY-FL-P 325 ±16 309 341
MO-FL-P 381 ±32 349 413
NE-SS-P 300 ±45 255 345
Note: NE-FS-P is preferred in the FS direction. LY-FL-P is preferred in the FL direction. NE-SS-P is preferred
in the SS direction.
Table 6-2 SD of Particle Swarms in DIFF Scattergram
Parameter Target Value Tolerance Lower Limit Upper Limit

NE-FS-SD 47.0 ±7.0 40.0 54.0
LY-FS-SD 63.0 ±9.4 53.6 72.4
MO-FS- SD 38.0 ±5.7 32.3 43.7
NE-FL- SD 74.0 ±11.1 62.9 85.1
LY-FL- SD 33.0 ±4.9 28.1 37.9
MO-FL- SD 35.0 ±5.2 29.8 40.2
NE-SS- SD 40.0 ±8.0 32.0 48.0
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Chapter 7 Component Adjustment and Detecting
.36 Component Adjustment and Parameter Setup

.36.1 Sensor AD Setup
Enter the “Menu” → “Service” → “Adjustment” → “Sensor AD” interface.
Add 50 to the reading after the input boxes for the cleanser sensor, diluent sensor, 86H
lyse sensor, and 86D lyse sensor, and input them to the respective input box.
Then click the “Setup” button under it.
.36.2 Debugging of Sampling Probe Position
1. Power on the machine and enter the “Menu” → “Service” → “Adjustment” → “Probe Pos”
interface.
2. DIFF Pos. (Horizontal) Debugging: Click the “Adjust” button, when “Move the probe to the
appropriate position,then click the ‘Detect’ button!” is popped up, put the tool of the DIFF bath
on the DIFF bath, and push the sampling probe into the hole of the tool manually. Then, click
“Detect” on the prompt box, and the sampling probe will be reset horizontally. Click “Read”,
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and the edit box there will display the corresponding number of steps. Click the “Setup”
button.
3. Debug the horizontal positions of the autoloader piercing position, WBC bath position,
RBC bath position, and enclosed puncture position by referring to Step 2.
4. Upper Position Debugging: Directly set to “0” and click the “Setup” button.
5. Isolation Bubble Establishment Position Debugging: Directly set to “100” and click the
“Setup” button.
6. WBC (RBC) Position (Vertical) Debugging: Click the “Adjust” button, when “Move the
probe to the appropriate position and click the ‘Detect’ button!” is popped up, put the tool of
the WBC bath on the WBC bath, and pull the sampling probe to the bottom of the tool
manually. Then, click “Detect” on the prompt box, and the sampling probe will be reset
vertically and the edit box there will display the corresponding number of steps. Add 400 to
the value in the edit box and input the new value to the edit box. Click “Setup” to finish the
debugging.
7. DIFF Bath Position (Vertical) Debugging: Click the “Adjust” button, when “Move the probe
to the appropriate position and click the ‘Detect’ button!” is popped up, put the debugging tool
of the DIFF bath on the DIFF bath, and pull the sampling probe to the bottom of the
debugging tool manually. Then, click “Detect” on the prompt box, and the sampling probe will
be reset vertically and the edit box there will display the corresponding number of steps. Add
460 to the value in the edit box and input the new value to the edit box. Click “Set up” to finish
the debugging.
8. Autoloader Sampling Position (Vertical) and closed Sampling Position (Vertical)
Debugging: Directly set to “800” respectively and click the “Setup” button.
9. First Piercing Position (Vertical) Debugging: Directly set to “400” and click the “Setup”
button.
.36.3 Waste Syringe Pressure Calibration
Enter the “Menu” → “Service” → “Adjustment” → “Pressure Calib.” interface; connect the
pressure gauge to the pipe joint on the top of the Waste Syringe.
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1. Positive Pressure Calibration: Click the “Reset” button on the interface, and input 100 to
the edit box with the unit of “AD”. Click the “Build” button, read the reading on the pressure
gauge connected to the pipe, and write it to the edit box with the unit of “KPa”. Click the “Add”
button. According to the above steps, test the pressure values 200, 400, 600, and 800
respectively. Then click the “Fit” button and click “Save” to finish the positive pressure
calibration.
2. Negative Pressure Calibration: Set the initial number of steps of negative pressure
establishment to “3500”. Click the “Reset” button on the interface, and input 100 to the edit
box with the unit of “AD”. Click the “Build” button, read the reading on the pressure gauge
connected to the pipe, and write it to the edit box with the unit of “KPa”. Click the “Add”
button. According to the above steps, test the pressure values 200, 400, 600, and 800
respectively. Then click the “Fit” button and click “Save” to finish the negative pressure
calibration.
.36.4 DIFF Bath Temperature Calibration

Power on the machine and enter the “Menu” → “Service” → “Adjustment” → “Performance”
interface. Add 4mL distilled water to the DIFF bath.
1. Input “30” to the first edit box and “0” to the second, and click “Set up”. Insert the
temperature sensor of the thermometer into the distilled water in the DIFF bath. Wait 5
minutes, and the reading of the thermometer should be “30±0.1” degrees.
2. If the reading is out of the range of “30±0.1”, the test value should be reduced by 30. Input
the calculation result to the second edit box and click “Setup” till the reading of the
thermometer is “30±0.1” degrees. Suck the distilled water in the DIFF bath with the pipette.
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.36.5 CBC Gain Debugging

1. Test a tpipe of QC blood or fresh human blood on the standard Analyzer three, calculate
the mean of the peak value of lymphocyte, MCV of RBC, and MPV of platelet as the
calibration reference value.
2. Conduct the test on the debugging instrument, and the test result should be basically
consistent with the calibration reference value (deviation of lymphocyte Peak: ±1.5FL;
deviation of RBC Peak: ±1FL). At the same time, the light intensity of HGB blank should be
between 2500-2900.
3. If the above results do not meet the requirements, adjust the gain values of the
corresponding items and click “Setup”. Retest the sample till the four items all meet the
requirements. Note: When the gain value increases, the light intensity of HGB, lymphocyte
peak, and RBC peak will increase.
.36.6 DIFF Gain Debugging

Enter the “Menu” → “Service” → “Adjustment” → “DIFF Gain (Particle)” interface.
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1. Dilute the standard particles into liquid with the total quantity of 3000~5000 for testing.
Requirements: the CV values of FS and FL are below 7%; the PK.AD value of FS is between
386~398; the PK.AD value of FL is between 423~439.
2. If the CV value of FS or FL does not meet the requirements, the inner and outer walls of
the sheath flow cell need to be cleaned.
3. If the PK.AD value of FS or FL does not meet the requirements, adjust he gains of the
corresponding items and click “Setup”. Retest the sample till the PK.AD values of FS and FL
meet the requirements.
Enter the “Menu” → “Service” → “Adjustment” → “DIFF Gain (Blood)” interface.
4. Test a pipe of QC blood or fresh human blood on the standard analyser thrice (the
differentiation charts of the various cells must be separated), calculate the mean of the center
of gravity in the FS direction of neutrophil, center of gravity in the FL direction of lymphocyte,
and center of gravity in the SS direction of neutrophil as the calibration reference value.
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5. Conduct the test on the debugging instrument, and the test result should be basically
consistent with the calibration reference value (deviation of center of gravity in all directions.
±10).
6. If the center of gravity value in the FS, FL or SS direction does not meet the requirements,
adjust the gain values of the corresponding items and click “Setup”. Retest the sample till the
values in the FS, FL and SS directions all meet the requirements.
.37 Test
.37.1 Functional Test
1. Earthing Check
Use the multimeter turned to 200Ω to test whether the resistance between all screws
and the ground terminal of the power supply on the rear side is less than 0.5 Ω. If it is greater
than 0.5, this item doesn’t meet requirement.
2. Software Version Check
Check whether the versions of the Information Processing System Application software,
main board software, driver board software, Autoloader board software, and Fluidics
sequence comply with the BOM (or contract review). If the versions are not compliant, this
item doesn’t meet requirement.
3. Sampling Probe Position Check
Enter the “Menu” → “Service” → “Adjustment” → “Probe Pos.” interface.
 Horizontal Position Check:
Automatic Puncture Position Check: The parameter should be “0”. If it is not “0”, this item
doesn’t meet requirement. After the check, click the “Assy Initialization” button.
DIFF Bath Position Check (Horizontal): Put the inspection tool into the hole in the middle
of the DIFF bath. Click the “Detect” button there and, when the sampling assy stops motion,
click the “V.motor Power-off” button. Pull down the sampling probe assy with hand, and the
sampling probe should to be inserted into the small hole of the tool. If it can’t be inserted into
the small hole, this item doesn’t meet requirement. After the check, click the “Assy Init.”
button.
WBC Bath Position Check: Put the inspection tool into the hole in the middle of the WBC
bath. Click the “Detect” button there and, when the sampling assy stops motion, click the
“V.motor Power-off ” button. Pull down the sampling probe assy with hand, and the sampling
probe should to be inserted into the small hole of the tool. If it can’t be inserted into the small
hole, this item doesn’t meet requirement. After the check, click the “Assy Init.” button.
RBC Bath Position Check: Put the inspection tool into the hole in the middle of the RBC
bath. Click the “Detect” button there and, when the sampling assy stops motion, click the
“V.motor Power-off” button. Pull down the sampling probe assy with hand, and the sampling
probe should to be inserted into the small hole of the tool. If it can’t be inserted into the small
hole, this item doesn’t meet requirement. After the check, click the “Assy Init.” button.
Upper Position: The parameter should be “0”. If it is not “0”, this item doesn’t meet
requirement.
Isolation Bubble Position Establishment Check: The parameter should be “100”. If it is
not “100”, this item doesn’t meet requirement.
WBC (RBC) Position Check: The parameter should be from 660-720. If it is outside the
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range, this item doesn’t meet requirement.

DIFF Bath Position Check (Vertical): The parameter should be from 660-700. If it is
outside the range, this item doesn’t meet requirement.
4. Syringe and Sampling Probe Motion Check
Enter the “Menu” → “Service” → “Mechanical Check” interface.
Click the sampling syringe, sample syringe, diluent syringe, Sheath fluid syringe, lyse
syringe, Waste Syringe, Probe H.motor, and Probe V.motor. The motor should operate
normally, without block or abnormal operating noise. If there is block or abnormal operating
noise, this item doesn’t meet requirement.
5. Waste Pump Function Check
Click the “Pump” button. The waste pump should be opened or closed normally, and the
operating noise should be normal. If it can’t be opened or closed, or has abnormal operating
noise, this item doesn’t meet requirement.
6. Valve Function Check
Select “All” for valves and click “Check”. There should be 26 sounds of valve opening and
closing. If the number of sounds is less than 26, this item doesn’t meet requirement.
7. Environment Temperature Check

Enter the “Menu” → “Service” → “System Status” interface.
The test value displayed of the environment temperature of the instrument should be the
actual environment temperature ±3. If it is out of the range, this item doesn’t meet
requirement.
8. DIFF Bath Temperature Check
Add 4mL distilled water to the DIFF bath, and insert the temperature sensor of the
thermometer into the distilled water in the DIFF bath. Wait 5 minutes, and the reading of the
thermometer should be “30±0.3” degrees. If it is out of the range of “30±0.3” degrees, this
item doesn’t meet requirement.
9. CBC Gain Check
Power off the machine, connect the diluent, cleanser, 86H lyse, 86D lyse, and waste
liquid pipes to the corresponding connectors of the instrument according to the colors on the
pipe joints, and connect the pipes to the corresponding reagent bottles. Power on the
machine, enter the “Service” → “Daily Maintenance” → “Fludics Maint.” interface, and click
“Prime”.
Test a pipe of QC blood or fresh human blood on the standard analyzer thrice, calculate
the mean of the peak value of lymphocyte and main peak value of RBC as the calibration
reference value. Conduct the test on the debugging instrument, and the test result should be
basically consistent with the calibration reference value (deviation of peak value of
lymphocyte: ±1.5FL; deviation of main peak value of RBC: ±1FL). At the same time, the light
intensity of HGB blank should be between 2900-3400. If the above results do not meet the
requirements, this item doesn’t meet requirement.
10. DIFF Gain Check
Enter the “Menu” → “Service” → “Adjustment” → “CBC Gain (Blood)” interface.
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Test a pipe of QC blood or fresh human blood on the standard analyzer thrice (the
differentiation charts of the various cells must be separated), calculate the mean of the center
of gravity in the FS direction of neutrophil, center of gravity in the FL direction of lymphocyte,
and center of gravity in the SS direction of neutrophil as the calibration reference value.
Compare the mean of the test results to the calibration reference value. The deviation of the
center of gravity in all directions should be ±10. If the above results do not meet the
requirements, this item doesn’t meet requirement.
11. Pressure Check
Enter the “Menu” → “Service” → “Adjustment” → “Pressure Calib” interface; connect the
pressure gauge to the pipe joint on the top of the Waste Syringe.
Positive Pressure Detect: Input “5” to the input box for positive pressure. Click “Reset”
and then “Detect”. The reading on the pressure gauge should be “5±1”Kpa. If it is outside the
range, this item doesn’t meet requirement. Negative Pressure Test: Input “15” to the input
box for negative pressure. Click “Reset” and then “Detect”. The reading on the pressure
gauge should be “-15±1”Kpa. If it is outside the range, this item doesn’t meet requirement.
12. Reagent Lack Alarm Function Check
Enter the “Menu” → “Service” → “Daily Maintenance” → “Replace Reagent” interface.
Remove the diluent pipe from the rear side of the instrument and click “Diluent”, and the
instrument will conduct diluent perfusion, during which, the status bar at the bottom of the
instrument will display the diluent lack. If there is no such prompt, this item doesn’t meet
requirement. After the check, connect the diluent pipe to the instrument and click “Diluent”
again, and the instrument will conduct diluent perfusion. Detect whether the cleanser, 86D
lyse, and 86H lyse are normal by referring to the above steps.
13. Waste Liquid Alarm Function Check
During the test, when the floating ball on the waste liquid sensor is pushed to the top, the
Information Processing System Software will prompt full waste liquid. If there is no such
prompt, this item doesn’t meet requirement.
14. Quantity Check of Optical Path Particle Test
Conduct the test on the test interface with the fresh human blood. When the test is
finished, click “Orig.Info.(O)”. The number of Non-ghost particles should be greater than 65%
of the number of WBC channels. If it is less than 60%, this item doesn’t meet requirement.
15. Prompt Sound and Alarm Sound Check
On the test interface, press the Liquid Suction Key for normal test. When the sample
suction is finished and the sampling probe is withdrawn, there should be prompt sound. If
there is no prompt sound or the prompt sound is abnormal, this item doesn’t meet
requirement.
.37.2 Performance Test
1. Blank Test: On the test interface, select the CBC+DIFF Whole Blood mode and conduct
the blank test. Three tests in row should meet these requirements: WBC ≤ 0.2, RBC ≤ 0.02,
PLT ≤ 10, and HGB ≤ 1. (Up to 10 tests can be conducted. If the requirements are not me et,
please treat the machine as one with faults).
2. Carry-over Test: On the test interface, select the CBC+DIFF Whole Blood mode, test the
fresh human blood or high-level QC thrice, and record the test results. Conduct 3 blank tests
and record the test results. When the tests are finished, calculate the carry-over with the
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carry-over formula. Requirements: WBC ≤ 0.5%, RBC ≤ 0.5%, PLT ≤ 1%, and HGB ≤ 0.5%.
Note: Requirements of high-level sample: WBC>15, RBC>6, PLT>200, and HGB>300. For
low-level samples, conduct blank tests.
3. Repeatability Test: On the test interface, select the CBC+DIFF Whole Blood mode. Test
the normal fresh human blood more than 1mL 10 times in a row and record the test results.
When the tests are finished, calculate the CV value with the repeatability formula. Test 3
tubes of human blood in a row. The mean of CV values of each tube of blood sample should
meet these requirements: WBC ≤ 2%, RBC ≤ 1.5%, PLT ≤ 4%, HGB ≤ 1.5%, MCV ≤ 1%,
MPV ≤ 4%, NEUT%±4, LYMP%±3, MONO%±2, EO%±1.5, and BA%±0.8, (If the repeatability
does not meet the requirements, conduct the set of tests again. If the repeatability still does
not meet the requirements, please treat the machine as one with faults).
4. Instrument Calibration: On the Manual Calibration interface, set the calibration parameters
in the Whole Blood mode and Pre-diluted mode to “1”. Test the normal-level QC blood more
than 2mL on the standard analyzer thrice and calculate the mean of WBC, RBC, HGB, PLT,
MCV and MPV as the target value of Quality Control.
① Calibration in Whole Blood Mode: On the Automatic Calibration interface, select the Whole
Blood mode, and input the target value of normal-level QC blood in WBC, RBC, HGB, PLT,
MCV, and MPV. Test the normal-level QC on the machine 5 times in a row. The CV values of
the 5 results must meet these requirements: WBC ≤ 2%, RBC ≤ 1.5%, PLT ≤ 4%, HGB ≤
1.5%, and MCV ≤ 1%. Save the results. If the CV values of the 5 results do not meet the
requirements, conduct the calibration again.
② Calibration in Pre-diluted Mode: On the Automatic Calibration interface, select the Pre-
diluted mode, and input the target value of normal-level QC blood in WBC, RBC, HGB, PLT,
MCV, and MPV. Click “OK” and then “Spit Diluent”. Put the tube under the sampling probe,
click the Liquid Suction Key 4 times, and the instrument will spit diluent. Take 80uL normal-
level QC blood with the 200uL pipette, wipe the outer wall of the head of the pipette clean,
add the QC to the tube with diluent spit by the instrument, and blend the contents well.
Conduct the test on the machine 5 times. The CV values of the 5 results must meet these
requirements: WBC ≤ 2%, RBC ≤ 1.5%, PLT ≤ 4%, HGB ≤ 1.5%, and MCV ≤ 1%. Save the
results. If the CV values of the 5 results do not meet the requirements, conduct the
calibration again.
5. Instrument Comparability Test: Test the QC blood more than 1mL on the standard analyzer
5 times and calculate the mean of the items as the reference value.
① On the test interface, select the CBC+DIFF Whole Blood mode, test the sample thrice,
and calculate the mean of the items. The deviation percentage between the mean of the
items and the reference value should meet these requirements: WBC±3%, RBC±2%,
HGB±2%, PLT±5%, and HCT±2%.
② On the test interface, select the CBC+DIFF Pre-diluted mode. Click “OK” and then “Spit
Diluent”. Put the tube under the sampling probe, press the Liquid Suction Key 4 times, and
the instrument will spit diluent. Take 80uL of the sample with the 200uL pipette, wipe the
outer wall of the head of the pipette clean, add the sample to the tube with diluent spit by the
instrument, and blend the contents well. Conduct the test on the machine thrice and calculate
the mean of the items. The deviation percentage between the mean of the items and the
reference value should meet these requirements: WBC±3%, RBC±2%, HGB±2%, PLT±5%,
and HCT±2%.
160
161
Chapter 8 Maintenance
.38 Regular Maintenance

Time Item Content of Maintenance
If the instrument is not powered off for 24 hours continuously, the
system will automatically prompt the operation of “Soaking in
Cleanser”.
Daily
When the accumulated number of samples tested reach 100pcs, the
system will automatically prompt the operation of “Soaking in
Cleanser”.
When the accumulated number of samples tested reach 400pcs or
Weekly every one week (7 days), the system will execute the operation of
“Socking in Concentrated Cleanser”.
Wipe the surface of the sampling probe, particularly the probe tip, with
Sampling
Monthly soft tissue manually at least once a month to remove the stubborn
probe
stains on the surface of the sampling probe.
Sampling Re-tension the horizontal and vertical belt pulleys to ensure the
assy positioning of the sampling probe is accurate.
Clean the bloodstain on the surface of the Rinse part and remove the
Semi- Rinse part
wear debris of the belt pulleys on the periphery of the Rinse part.
annually
Waste
Apply (special) lubricant on the plunger of the Waste Syringe.
Syringe
All syringes Apply lubricant on the 6 syringe drive screws of the whole machine.
On Counting When the counting chamber is contaminated, the “Clean *** Counting
Demand chamber Chamber” operation is executed.
When the center of gravity of the scattergram is outside the specified
Flow Cell range or the CV value of the particle test is greater than 7%, the “DIFF
Channel Soaking in Concentrated Cleanser” operation is executed.
Ruby When the Ruby aperture is blocked, on the Daily Maintenance interface,
aperture the ”Unclog”, “Zap Apertures” or “Back Flush” operation is executed.
162
Filter To be replaced in case of blockage or leakage of the Rinse part.
Before long-distance transport or the analyzer is out of service for more
Out of than 3 days, execute the “Pack” operation in the “Service” menu to
service clean and drain the Fluidics of the analyzer, wipe the analyzer dry, and
put it in a clean place.
If there are other fault messages, refer to “Treatment” to make the

Alarm
corresponding treatment.
163
Chapter 9 Clinical Alarm
.39 Alarm Flags

.39.1 There is no parameter result
When the test is finished, no parameter result comes out, a certain flag will appear:
Table 3 Flags Substituting Parameter Values
Flag Description
::::: Hole blockage
The parameter is invalid or can’t be
-----
calculated.
+++++ The parameter is out of the display range.
.39.2 Parameter result with alarm flag
The parameter value alarm flag and the parameter result appear at the same time. The
specific definitions are show inTable 4.
Table 4 Parameter Value Alarm Flag
Flag Description
+ The parameter is outside the measurement range.
* A suspicious flag of parameter indicates the credibility of the
parameter result is not high. The suspicious flags of the
various parameters are as follows:
Parameter Possible Causes
WBC  Poor hemolysis
 Nucleated red blood cells, large PLT / PLT
clumps interference
 Electrical noise and bubble interference
 Hole blockage
DIFF5%  Poor hemolysis of DIFF channel
 Low-level white blood cell
 Nucleated red blood cells, large PLT / PLT
clumps interference
 WBC excessive dissolved
 Dirty or blocked Flow Cell
HGB  The instrument has not been calibrated.
 High white blood cell interference, high
lipid blood sample
 Abnormal hemoglobin voltage
RBC  Large PLT / PLT clumps interference
 Small red blood cell interfered by platelet
 Abnormal red blood cell distribution
164
 Hole blockage
PLT  Large PLT / PLT clumps
 Small red blood cell
 Abnormal platelet distribution
 Hole blockage
Note: DIFF5% refers to the 5-part differentiation parameters, i.e. NEUT%, LYMP%, MONO%, EO%,
and BASO%.
.39.3 Alarm Messages

Alarm messages explain results with alarm. The specific contents are shown in the table
below:
Table 5 Alarm Messages
Type Alarm Message Possible Causes
WBC Flag WBC Scattergram  The sample is abnormal.

Abnormal  The Flow Cell is dirty or blocked.
 The hemolysis is poor.
 There is nucleated red blood cells, large PLT /
WBC Histogram
PLT clumps interference.
Abnormal
 There is electrical noise and bubble interference.
 The hole is blocked.
Leukocytosis WBC is greater than the set value.
Leukocytopenia WBC is less than the set value.
Neutrophilia NEUT# is greater than the set value.
Neutropenia NEUT# is less than the set value.
Lymphocytosis LYMP# is greater than the set value.
Lymphopenia LYMP# is less than the set value.
Monocytosis MONO# is greater than the set value.
Eosinophilia EO# is greater than the set value.
Basophilia BASO is greater than the set value.
In the DIFF scattergram, the number of particles in the
Blasts?
Blasts area is big.
In the DIFF scattergram, the number of particles in the
Immature Gran?
Immature Gran area is big.
Left Shift? In the DIFF scattergram, the number of particles in the
Left Shift area is big.
Atypical/Abn In the DIFF scattergram, the number of particles in the
165
Lympho? Atypical/Abn Lympho area is big.

RBC Lyse In the DIFF scattergram, the number of undissolved
resistance? RBCs is big.
 There is large PLT / PLT clumps interference.
RBC Distribution  The small RBC interfered by PLT.
Abnormal  There is electrical noise and bubble interference.
The sample is abnormal, for example, the patient is
Dimorphic
under treatment of iron deficiency or under blood
Population
transfusion, etc.
Erythrocytosis RBC is greater than the set value.
Anemia HGB is less than the set value.
RBC Flag
Anisocytosis RDW-SD/RDW-CV is greater than the set value.
Macrocytosis MCV is greater than the set value.
Microcytosis MCV is less than the set value.
Hypochromia MCHC is less than the set value.
RBC
RBC channel parameters are abnormal.
Agglutination?
HGB
RBC channel parameters are abnormal.
interference?
Iron Deficiency? RBC channel parameters are abnormal.
 There is large PLT / PLT clumps.
PLT Distribution  Small RBC
Abnormal  There is electrical noise and bubble interference.
PLT Flag
Thrombocytosis PLT is greater than the set value.
Thrombocytopenia PLT is less than the set value.
The PLT histogram is abnormal, for example, the
PLT Clumps?
distribution is wide or the tail of the histogram is high
.40 Histogram
This section gives some WBC histograms frequently seen in actual applications.
1. Normal histogram
2. The histogram is too narrow (Abnormal sample? Small WBC gain? Aged sample?)
166
3. There is interference at the front end of histogram (Poor hemolysis, nucleated red
blood cells, large PLT / PLT clumps, electrical noise, etc.)
4. Hole blockage of WBC
167
Chapter 10 Faults and Treatment
.41 Overview of Fault Messages
Fault Fault Fault

Fault Name Fault Name Fault Name
Code Code Code
Dr i v e r b o a r d
1001 communicatio 1023 No 86H lyse 1045 Autolader board communication error
n error
Pressure O p e n n i n g s a m p l e c o m p a r t
1002 1024 No 86D lyse 1046
abnormal failed
Diluent
syringe W a s t e i s
1003 1025 1047 Sample compartment door not closed
action full
abnormal
Sample
injection
Diluent
1004 syringe 1026 1048 Loading motor action abnormal
expired
action
abnormal
Sheath
fluid Cleanser
1005 syringe 1027 expired 1049 Feeding motor action abnormal
action
abnormal
Lyse
syringe 8 6 H l y s e
1006 1028 1050 Elevating motor action abnormal
action expired
abnormal
Sampling
syringe 8 6 D l y s e
1007 1029 1051 Pinch motor action abnormal
action expired
abnormal
Waste
syringe
1008 1030 WBC clog 1052 Mix motor action abnormal
action
abnormal
1009 1031 RBC clog 1053 Unloading motor action abnormal
Pressure of
sheath
S c a n n e r e l e v a t i n g m o
1010 fluid 1032 1054
abnormal
exceeds the
limit
1011 1033 1055 No tube rack on loading platform
168
Sheath
fluid Left door
1012 1034 1056 Autoloader module action abnormal
pipeline open
leaks
Sample
Right door
1013 pipeline is 1035 1057 Mix module action abnormal#1057
open
blocked
Sample
Front
1014 pipeline 1036 1058 Mix module action abnormal#1058
shell open
leaks
Temperatur
F l o w c e el le x c e e d s
1015 1037 1059 Mix module action abnormal#1059
blocked working
range
DIFF
External
reaction
drain
1016 1038 bath 1060 Mix module action abnormal#1060
pipeline
temperatur
blocked
e abnormal
Liquid Probe
drain horizental
1017 1039 1061 Mix module action abnormal#1061
pipeline action
leaks abnormal
Probe
B a c k b a t h
vertical
1018 pipeline 1040 1062 Unloading tube rack error
action
leaks
abnormal
Positive
pressure
1019 1041 1063 Unloading tray is full
establishmen
t failed
Negative
pressure
1020 1042 1064 Scan action abnormal
establishmen
t failed
1021 No cleanser 1043 1065
1022 No diluent 1044 1066
169
.42 Whole Machine Treatment

Fault
Fault Name Causes Steps of Treatment
Code
1. Click the “Clear fault” button to execute assy
initialization.
Driver board The serial port 2. Turn off the power of the instrument and
1001 communicatio communication is restart it later.
n error abnormal. 3. If the fault still exists, check the
communication related sections of the driver
board.
initialization.
1. Fault of valve
Pressure 2. If the fault still exists, check whether the
1002 2. Fault of driver
abnormal valve SV22 is normal.
board
3. Check the air pressure test related sections of
the driver board.
Diluent
syringe
1003
action
abnormal
Sample
injection 1. Click the “Clear fault” button to execute assy
1004 syringe initialization.
action 1. Fault of 2. If the fault still exists, check whether the
abnormal optocoupler test optocoupler test section of the related syringe
Sheath fluid 2. Mechanical assy is normal.
syringe structure fault of 3. If the fault still exists, check whether the
1005
action syringe assy motor drive and mechanical drive sections of the
abnormal 3. Fault of body of related assy are normal.
Lyse syringe syringe (resistance 4. If the fault still exists, check whether the
1006 action is too big) resistance of the body of syringe is too big
abnormal 4 Fault of driver (crystallization or seal ring damage may cause
Sampling board syringe sticking).
syringe 5. If the fault still exists, check whether the
1007
action related sections of the driver board are normal.
abnormal
Waste
syringe
1008
action
abnormal
1009
Pressure of 1. Blockage of Flow 1. Check whether the valve ports of the valves
1010
sheath fluid Cell SV5, SV6, SV7 and SV13 and the related pipes
170
of the Flow Cell have fallen off. Click the “Clear

2. Fault of valve
fault” button to clean the Flow Cell.
(alarm during gas
2. If the fault still exists, unplug the inlet pipe of
tightness test upon
the valve SV25 and add distilled water to the
power-on may
pipe with the manual syringe to dredge the Flow
indicate the liquid in
exceeds the Cell (you can observe whether the optical test
the sheath flow pipe
limit area of the Flow Cell has any foreign matter).
has not been
3. If the fault still exists, check whether the
drained)
valves SV4, SV6, SV7, and SV25 are normal.
3. Fault of liquid
4. If the fault still exists, replace with a new
pressure sensor or
liquid pressure sensor or check the related
driver board
sections of the driver board.
1011
1. Check whether the valve port related pipes of
1. Fall-off of pipe
the valves SV4, SV5, SV6, SV7, and SV13
2. Fault of valve
have fallen off.
(SV4, SV5, SV6,
Sheath fluid 2. If the pipes are connected correctly, check
and SV7 leak; SV13
1012 pipeline whether the valves SV4, SV5, SV6, SV7, and
is not opened)
leaks SV13 are normal.
3. Fault of liquid
pressure sensor or
driver board
1. Fault of valve 1. Click the “Clear fault” button to clean the Flow
(the liquid in the Cell.
Sample sample pipe has not 2. If the fault still exists, check whether the
1013 pipeline is been drained) valves SV1, SV4, and SV6 are normal.
blocked 2. Fault of liquid 3. If the fault still exists, replace with a new
pressure sensor or liquid pressure sensor or check the related
driver board sections of the driver board.
1 Check whether the valve ports of the valves
1. Fall-off of pipe
SV1, SV4, and SV25 and the Flow Cell related
2 Fault of valve
pipes have fallen off.
Sample (SV25 leaks; SV29
2 If the pipes are connected correctly, check
1014 pipeline can’t be closed)
whether the valves SV25 and SV29 are normal.
leaks 3. Fault of liquid
pressure sensor or
driver board
1015 Flow cell 1. Flow Cell 1. Click the “Clear fault” button to clean the Flow
blocked blockage Cell.
2. Fault of valve 2. If the fault still exists, unplug the inlet pipe of
(SV25 is not the valve SV25 and add distilled water to the
opened) pipe with the manual syringe to dredge the Flow
3. Fault of liquid Cell (you can observe whether the optical test
pressure sensor or area of the Flow Cell has any foreign matter).
171

valve SV25 is normal.
driver board 4. If the fault still exists, replace with a new
1. The waste liquid
pipe of the 1. Firstly check whether the waste liquid pipe is
instrument is not connected correctly.
connected. 2. Check whether the valve SV32 is normal.
External
2. Fault of valve 3. Remove the liquid pump and remove the
drain
1016 (SV32 is not impurities in the pump head to clear the fault. If
pipeline
opened) the fault still exits, replace with a new liquid
blocked
3. Blockage of liquid pump.
pump 4. Check the air pressure test related section of
4 Fault of driver the driver board.
board
1. Check whether any of the drain pipe
connections between the SV30 → SV26 →
1. Fall-off of pipe SV27 → SV28 → SV32 valves has fallen off.
Liquid drain
2. Fault of valve 2. If the pipes are connected correctly, check
1017 pipeline
3. Fault of driver whether the valves SV26, SV27, SV28, and
leaks
board SV32 are normal.
3 Check the air pressure test related section of
the driver board.
1. Check whether any of the pipe connections
1. Fall-off of pipe
between SV21 →back bath for RBC counting
2. Damage of
vial →back bath for WBC counting vial →
counting vial (leak
Back bath SV24 → WA syringe has fallen off.
of back bath,
1018 pipeline 2. Test whether the back bath for counting vial
damage of Ruby
leaks is tight or whether the Ruby Aperture is
Aperture)
normal.
3. Fault of driver
3. Check the air pressure test related section of
board
the driver board.
1019 Positive 1. Fall-off of pipe 1. Check whether the connection of the Waste
pressure 2. Fault of valve Syringe outlet related pipe has fallen off. Click
establishment 3. Fault of pressure the “Clear fault” button to execute assy
failed calibration initialization and positive pressure test.
4. Fault of driver 2. If the pipe connection is normal, check
board whether the valves SV22, SV23, SV24, and
SV30 are normal.
3. Enter the “Service → Adjustment → Pressure
Calib” interface, and recalibrate the pressure
coefficient.
172
the driver board.

1. Firstly, check whether the connection of the
Waste Syringe outlet related pipe has fallen off.
Click the “Clear fault” button to execute assy
initialization and negative pressure test.
1. Fall-off of pipe 2. Check, during the CBC channel testing,
2. Lack of diluent whether the liquid level in the WBC/RBC front
3. Fault of valve bath is higher than the Ruby Aperture and
Negative
4. Fault of whether the back bath pipe is full of liquid.
pressure
1020 optocoupler 3. Check whether the valves SV22, SV23,
establishment
5 Fault of pressure SV24, and SV30 are normal.
failed
calibration 4. Test whether the optocoupler of the Waste
6. Fault of driver Syringe is normal.
board 5. Enter the “Service → Adjustment → Pressure
Calib” interface, and recalibrate the pressure
coefficient.
the driver board.
1. Check whether there is any reagent in the
1021 No cleanser
reagent bottle corresponding to the alarm
message. If no, replace with a new bottle of
1022 No diluent
reagent. Then click the “Clear fault” button to
1. No reagent
execute the “Replace *** Reagent” operation.
1023 No 86H lyse 2. Fault of liquid
2. If there is much remaining reagent as found in
level sensor (the
the check, firstly check whether there is bubble
settings of pipe
in the pipe of the liquid level sensor for the
bubble and AD
reagent corresponding to the alarm message. If
value are
no, enter the “Service → Adjustment → Sensor
inappropriate)
AD” interface to check whether the AD value
1024 No 86D lyse 3. Fault of driver
setting complies with the standard (the AD
board
threshold is between the full and no reagent in
the pipe).
3. Check the liquid level sensor test related
section of the driver board.
1025 Waste is 1. The waste liquid 1. Replace with a new waste liquid container or
full container is full. drain the waste liquid.
2. The waste liquid 2. Check whether the waste liquid sensor cable
sensor cable is not on the rear side of the instrument is connected
connected. correctly.
3. The waste liquid 3. If the waste liquid container is not full,
sensor cable or the measure the waste liquid sensor (NC type) with
sensor is damaged. a multimeter. If the circuit is closed when floater
4. Fault of driver is on the bottom and open when floater is at the
board top, it indicates that the waste liquid sensor is
173
normal. Otherwise, check whether the sensor

cable or the sensor is damaged.
4. Check the related section of the driver board.
Diluent
1026 1. Firstly, check whether the reagent
expired
1. The reagent is corresponding to the alarm message is expired.
Cleanser
1027 expired. If yes, replace with a new bottle of reagent.
expired
2. The shelf life of Then click the “Clear fault” button to execute the
86H lyse
1028 the reagent is not “Replace *** Reagent” operation.
expired
set properly. 2. If the reagent is not expired, reset the shelf
86D lyse
1029 life of the reagent.
expired
1. Click the “Clear fault” button to execute the
1030 WBC clog blockage removal operation.

2. Execute the “Back Flush“ operation, observe
whether there is liquid jetting to the inner wall
opposite to the front bath of the counting vial
through the rear bath Ruby Aperture during the
back flush. If no, continue to execute
1. The Ruby Unclog till the Ruby Aperture is
Aperture is blocked. through.
3. If the operation executed can’t get through
1031 RBC clog
the Ruby Aperture, unplug Opening 1 of the
back bath of the WBC bath or RBC bath, push
and pull the back bath pipe with the manual
syringe, and get through the Ruby Aperture.
Ruby Aperture.
1032
1033
Left door 1. The side door of 1. Close the related side door, and the fault will
1034
open the instrument is disappear automatically.
Right door opened. 2. If the fault still exists, test the microswitch
1035
open 2. Fault of sensor once by pressing the door with hand to check
test whether the microswitch test section is normal.
Front shell
1036 3. Fault of main 3. Check the related section of the main control
open
control board board.
1037 Temperature 1. The environment 1. Ensure the environment temperature is in the
exceeds temperature is out work temperature range [15-30]℃. If the
working of the usable range environment temperature is out of the normal
range of the instrument. range, the test result may be inaccurate.
2. Fault of 2. If the environment temperature is in the
environment normal range, check whether the environment
temperature test temperature sensor is normal.
sensor 3. Check the related section of the driver board.
174
3. Fault of driver
board
1. Click the “Clear fault” button to reconfigure
the temperature of the DIFF bath. If the
1. The temperature temperature of the DIFF bath is too high,
setting of the DIFF execute the “Clean DIFF Bath” operation
bath is abnormal. several times. If the temperature is still
DIFF
2. Fault of DIFF abnormal, turn off the power of the instrument
reaction
bath temperature and restart it later.
1038 bath
sensor or 3. If the fault still exists, unplug the two-way plug
temperature
temperature connector of the heating wire at the bottom of
abnormal
protection switch the DIFF bath and measure whether the
3. Fault of driver heating element is normal with a multimeter (if
board the resistance is infinite, it indicates that the
heating element of the DIFF bath is damaged).
4. Check the related section of the driver board.
1 Fault of
optocoupler test
initialization.
2. Fault of horizontal
driving of sampling
Probe horizontal optocoupler test section of the
assy (fault of motor,
horizental sampling assy is normal.
1039 big resistance of
action 3. If the fault still exists, check whether the
horizontal driving
abnormal horizontal motor drive and mechanical drive
block, wear of belt
sections of the sampling assy are normal.
pulley)
4. Check whether the related section of the
3. Fault of driver
driver board is normal.
board
1. Fault of initialization.
optocoupler test 2. If the fault still exists, check whether the
Probe 2. Fault of vertical vertical optocoupler test section of the sampling
vertical driving of sampling assy is normal.
1040
action assy (fault of motor, 3. If the fault still exists, check whether the
abnormal wear of belt pulley) horizontal motor drive and mechanical drive
3. Fault of driver sections of the sampling assy are normal.
board 4. Check whether the related section of the
driver board is normal.
.43 Alarm Treatment for Automatic Sampler Section

Fault Alarm Fault
Causes Steps of Treatment
Code Text Name
1045 Autolader board The serial port 1. Click “Clear fault” to empty the tube
communication communication is rack and restart Automatic Sampling.
error abnormal. 2 Turn off the power of the instrument
175
and restart it later.

3. If the fault still exists, check whether
the communication related section of the
automatic sampling driver board is
normal.
1. If the sample compartment door is not
opened, click “Clear fault” to open the
sample compartment door once again.
2. If the sample compartment door can’t
1. The electromagnet is be opened, measure whether the
damaged. resistance of the electromagnet is about
Openning sample 2. Fault of optocoupler 24Ω with a multimeter.
1046 compartment door test of sample 3. If the sample compartment door is
failed compartment open, check whether the optocoupler test
3. Fault of automatic section of the sample compartment door
sampler driver board is normal.
the drive of the related section of the
normal.
2. If the sample compartment door can’t
be closed, adjust the distance between
1. The distance between the enclosed sampling adapter
the position of the mechanism and the shell to make the two
sample compartment in normal contact.
and the shell is not 3. If the sample compartment door still
adjusted properly. can’t be closed, check whether the stop
2. Fault of mechanical plate of the e sample compartment is
Sample compartment structure damaged and whether the mandrel of the
1047
door not closed 3. Fault of optocoupler electromagnet can protrude normally.
test of sample 1. If the sample compartment door is
compartment closed, check whether the optocoupler
4. Fault of automatic test section of the sample compartment
sampler driver board door is normal.
(the electromagnet is 4. If the fault still exists, check whether
kept powered on) the drive of the related section of the
normal.
1048 Loading motor action 1. Fault of optocoupler 1 Click the “Clear fault” button to execute
abnormal test initialization of the related component.
1049 Feeding motor 2. Fault of mechanical 2 If the fault still exists, check whether
action abnormal structure the optocoupler test section of the related
1050 Elevating motor 3 Fault of automatic assy is normal.
action abnormal sampler driver board 3. If the fault still exists, check whether
176
Pinch motor action

1051
abnormal
Mix motor action
1052
abnormal
Unloading motor
1053 the motor drive and mechanical drive
action abnormal
sections of the related assy are normal.
Scanner elevating
1054 motor action
the drive of the related section of the
abnormal
1. Firstly, check whether the tube rack is
placed correctly and restart Automatic
Sampling.
2. If the fault still exists, start Automatic
Sampling and press the microswitch with
The steps of load & hand once to check whether the
propulsion motor are microswitch test section is normal.
No tube rack on
1055 improperly or the position 3. If the microswitch test section is
loading platform
of tube rack detecting normal, enter the “Service → Adjustment
microswitch is offset. → Autoloader” interface and readjust the
maximum number of stroke steps of the
“Loading motor”. If, after the number of
steps has been set to the maximum, the
fault still can’t be cleared, adjust the
installation position of the microswitch.
1. The tube rack is not
1. Remove the tube rack or other items in
placed properly or the
the sampling area and recycling area and
sampling area has
click “Clear fault” to empty the tube rack.
foreign matter.
2. The tube rack is
the bottom of the tube rack is damaged.
Autoloader module damaged.
1056 3 If the fault still exists, check whether
action abnormal 3. Fault of optocoupler
the optocoupler section for the tube rack
for tube rack left/right
left/right motion test is normal.
motion test
4. Check whether the pusher dog
4. Fault of mechanical
protrudes normally when the sampling
structure of sampling
propulsion mechanism is operating.
propulsion motor
1057 Mix Drop of 1. The tube drops. 1. Firstly, check whether any tube drops.
module tube during 2. Fault of optocoupler If yes, take out the tube manually, click
action code scan test the “Clear fault” button to initialize the
abnormal automatic sampler, and restart Automatic
#1057 Sampling.
the tube has a test optocoupler and
whether the test optocoupler at 1#
177
blending position is normal.

Mix
Drop of 1. Firstly, check whether any tube has
module
tube at 1# dropped. If yes, take out the tube
1058 action
blending manually, click the “Clear fault” button to
abnorma 1. The tube drops.
position initialize the automatic sampler, and
l#1058 2. Fault of optocoupler
restart the sampling.
Mix test at the blending
Drop of 2 If the fault still exists, check whether
module position
tube at 2# the tube has a test optocoupler and
1059 action
blending whether the test optocoupler at 1#
abnorma
position blending position is normal.
l#1059
Mix 1. The jack catch is not
Tube at 1# 1 Click the “Clear fault” button to initialize
module at the initial position
blending the automatic sampler, and restart the
1060 action when the tube is to be
position not sampling.
abnorma grabbed.
grabbed 2. If the fault still exists, check whether
l#1060 2. Fault of optocoupler
the test optocoupler at the related
test at the blending
blending position is normal.
position
Mix 3. The number of steps
Tube at 2# the jack catch aligns at the tube at the
module for reset of the blending
blending blending position after the tube blending
1061 action motor is not set properly.
position not moror is reset.
abnorma 4. The installation
grabbed 4. If the fault still exists, check whether
l#1061 position of the blending
the jack catch aligns at the tube at the
mechanism is
initial position of the mechanism.
inappropriate.
1. The tube rack in the
recycling area is placed
the recycling area and click “Clear fault”
wrongly or the recycling
to empty the tube rack.
area has foreign matter.
Unloading tube rack 2. Fault of mechanical
1062 the mechanical drive of the recycling
error structure of recycling
propulsion mechanism is normal.
propulsion mechanism
3. Fault of test
the test optocoupler 1 in recycling area is
optocoupler 1 in
normal.
recycling area
1. The recycling area is
full or the optocoupler is
the recycling area and click the “Clear
covered by foreign
Unloading tray is fault” button.
1063 matter.
full 2. If the fault still exists, check whether
2 Fault of test
the test optocoupler 2 in recycling area is
optocoupler 2 in
normal.
recycling area
Scan action 1. The reflective 1. Check whether the reflective
1064
abnormal optocoupler test of code optocoupler of code scan is normal.
178
2. If yes, toggle up the press head of the

scan is abnormal.
tube, and check whether the press head,
2. The position of the
after moved up, is able to fully cover the
reflective optocoupler of
optocoupler. If no, adjust the installation
code scan is offset.
position of the optocoupler.
.44 Treatment of Other Common Faults of Analyzer

S/ Fluidics
Possible Causes Solutions
N Fault
1. Firstly, check whether the waste liquid pipe of the
instrument is connected correctly.
1. The waste liquid pipe
2. Enter the “Service → Mechanical Check” interface
is not connected.
and test whether the valve SV31 works normally.
2. Fault of SV31 valve
3. Replace with a new filter screen.
3. The filter screen is
Rinse part 4. Enter the “Service → Mechanical Check” interface
1 blocked.
leak and test whether the liquid pump works normally. If the
4. The liquid pump is
liquid pump can’t work, replace with a new one to clear
blocked or damaged.
the fault. If the liquid pump can run, separate the pump
5. The Rinse part is
head and remove the impurities to clear the fault.
blocked or damaged.
5. Clean the Rinse part and conduct the test again. If
the fault still exists, replace the Rinse part.
1. The waste liquid pipe 2. Enter the “Service → Mechanical Check” interface
is not connected. and test whether the valves SV28 and SV3 work
DIFF bath 2. Fault of SV28 and normally.
2
leak SV32 valves 3. Enter the “Service → Mechanical Check” interface
3. The liquid pump is and test whether the liquid pump works normally. If the
blocked or damaged. liquid pump can’t work, replace with a new one to clear
1. The waste liquid pipe
is not connected.
and test whether the valves SV27 and SV32 work
2. Fault of SV27 and
normally.
WBC bath SV32 valves
3 3. Enter the “Service → Mechanical Check” interface
leak 3. The liquid pump is
and test whether the liquid pump works normally. If the
blocked or damaged.
4. The counting vial is
damaged.
4. Replace with a new WBC bath counting vial.
4 RBC bath 1. The waste liquid pipe 1. Firstly, check whether the waste liquid pipe of the
179

is not connected.
2. Fault of SV26 and
normally.
SV32 valves
leak 3. The liquid pump is
and test whether the liquid pump works normally. If the
blocked or damaged.
4. The counting vial is
damaged.
4. Replace with a new RBC bath counting vial.
and click “Waste Syringe” to test whether the motion of
the Waste Syringe is normal.
and test whether the bubble blending control valve
1. Fault of Waste
SV30 and the drain valves SV26, SV27, and SV28
Syringe
corresponding to the three baths work normally.
No bubble 2. Fault of valve
comes out 3. Fall-off of pipe
and click “Neg. Pressure Test”. If there is no alarm, the
5 during 4. The pipe is blocked.
system will prompt that the pipe connection is normal.
bubble 5. Fault of pressure
4. Check whether the drain pipe and bubble blending
blending calibration (the pressure
pipe (the pipe between Opening 4 of the Waste Syringe
for bubble blending is
and outlet of the SV30 valve) of the counting chamber
too low)
have been drained before bubble blending.
5. Check whether the pressure for bubble blending is in
the range of “4~7Kpa”. If no, enter the “Service →
Adjustment → Pressure Calib” interface and recalibrate
the pressure coefficient.
1. Firstly, observe whether the bath has been drained
before the counting vial is added with liquid. If no,
1. Fault of liquid drain check whether the drain valves SV26, SV27, SV28,
Liquid
2. Contamination of and SV32 corresponding to the baths are normal and
overflows
counting chamber whether the liquid pump is normal.
the
(residue of lyse) 2. Execute the “Clean *** Counting Chamber” operation
6 counting
3. Fault of pressure or clean the inner wall of the related counting
vial after
calibration (the pressure chamber with soft tissue manually.
bubble
for bubble blending is 3. Test whether the pressure for bubble blending is in
blending
too high) the range of “4~7Kpa”. If no, enter the “Service →
Adjustment → Pressure Calib” interface and recalibrate
the pressure coefficient.
7 Abnormal 1. The counting chamber 1. Enter the “Service → Daily Maintenance → Clean”
blank test is contaminated or the interface, execute the “Fluidics Clean” or “Cleanser
Fluidics has bubble. sock” operation, and conduct the blank test again.
2. The diluent is 2. Take a new container of diluent, enter the “Service →
180
Daily Maintenance → Rplc. Reagent” interface,

execute “Diluent”, and conduct the blank test again.
contaminated or expired. 3 Enter the “Service → Daily Maintenance → Upkeep”
3. The Ruby Aperture is interface, execute the “Unclog” operation, check that
blocked. the Ruby Aperture is not blocked, and conduct the
4. Noise interference blank test again.
4. Ensure the analyzer is far away from interference
sources.
1. The laser lamp is not
turned on or is
1. Firstly, check whether the laser lamp is normal.
damaged.
2. Fault of valve (DIFF
and check whether the diluent syringe and the valve
No data on bath sample is not
SV1 work normally.
8 DIFF prepared)
channel 3. Fault of sample
and check whether the sample syringe works normally.
syringe
4. Check the related sections of the signal receiving
4. Fault of signal
plate and the main control board.
receiving plate or main
control board
1. If the sample is not prepared properly, enter the
1. Fault of diluent
“Service → Mechanical Check” interface and check
syringe (the sample is
whether the diluent syringe works normally (whether it
Too few not prepared properly)
leaks) and whether the valve SV1 works normally.
particles in 2. Fault of valve (no
9 2. Enter the “Service → Mechanical Check” interface
DIFF auxiliary sample pushing
and check whether the valve SV4 works normally.
channel process)
3. Fault of sample
and check whether the sample syringe works normally
syringe quantification
(whether it leaks).
1. The operation is
nonstandard, and the
1. Shake the sample well and retest it.
sample is not fully
2. Select an appropriate sample and conduct the test
blended.
again.
2. The sample is not well
3. Enter the “Service → Daily Maintenance → Clean”
Poor selected (with blood clot
interface, execute the “Fluidics Cleaning” operation,
repeatabilit or abnormal blood).
10 and conduct the test again.
y of most 3. The testing chamber
4. Take a new container of diluent, enter the “Service →
indicators is contaminated or the
Daily Maintenance → Rplc. Reagent” interface,
Fluidics system has
execute “Diluent”, and conduct the test again
bubble.
4. The reagent is
sources.
contaminated or expired.
5. Noise interference
181
1. Select an appropriate sample of WBC and conduct

the test again.
2. Enter the “Service → Daily Maintenance → Upkeep”
interface, execute the “Back Flush” operation, and
observe whether there is liquid jetting to the inner wall
opposite to the front bath of the counting vial through
selected (blood with
the rear bath Ruby Aperture during the back flush. If
abnormal WBC).
no, execute the “Blockage Removal” operation till the
2. The Ruby Aperture of
Ruby Aperture is through.
the WBC bath is
blocked.
interface, execute the “Clean WBC Bath” operation,
3. The WBC bath is
and conduct the test again. If, after blended, the liquid
Poor contaminated (the
still overflows the vial, clean the inner wall of the WBC
11 repeatabilit bubble blending is
bath with soft tissue manually.
y of WBC abnormal; the liquid
4. Open the shield of the counting vial, observe
overflows the vial).
whether the bubble blending in the WBC bath is
4. There is no bubble in
normal. If no bubble comes out, make treatment by
the WBC bath.
referring to “No bubble comes out during bubble
5. Fault of 86H lyse
blending” described above.
6. The WBC gain setting
5. Check whether the 86H lyse is used up, whether the
is inappropriate.
valve SV12 works normally, and whether the
7. Noise interference.
quantification of the 86H lyse syringe is accurate.
6. Enter the “Service → Adjustment → CBC
Gain(Blood)” interface and readjust the WBC gain.
sources.
12 Poor 1. The WBC bath is 1. Enter the “Service → Daily Maintenance → Clean”
repeatabilit contaminated (the interface, execute the “Clean WBC Bath” operation,
y of HGB bubble blending is and conduct the test again. If, after blended, the liquid
abnormal; the liquid still overflows the vial, clean the inner wall of the WBC
overflows the vial). bath with soft tissue manually.
2. There is no bubble in 2. Open the shield of the counting vial, observe
the WBC bath. whether the bubble blending in the WBC bath is
3. Fault of 86H lyse normal. If no bubble comes out, make treatment by
4. The HGB gain setting referring to “No bubble comes out during bubble
is inappropriate. blending” described above.
5. The electrode plate in 3. Check whether the 86H lyse is used up, whether the
the WBC bath is valve SV12 works normally, and whether the
covered. quantification of the 86H lyse syringe is accurate.
6. The LED is loose or 4. Enter the “Service → Adjustment → CBC
damaged. Gain(Blood)” interface and set the HGB gain to make
the HGB blank value be about 2900.
5. Open the shield of the WBC bath and move the
electrode plate in the WBC bath to the wall of the
182
counting vial near the front shell.

6. Check whether the LED is installed correctly.
1. Select an appropriate sample of WBC and conduct
the test again.
interface, execute the “Back Flush” operation, and
observe whether there is liquid jetting to the inner wall
opposite to the front bath of the counting vial through
selected (blood with
the rear bath Ruby Aperture during the back flush. If
abnormal RBC/PLT)
no, execute the “Unclog” operation till the Ruby
2. The Ruby Aperture is
Aperture is through.
Poor blocked.
repeatabilit 3. The RBC bath is
13 interface, execute the “Clean RBC Bath” operation, and
y of contaminated.
conduct the test again.
RBC/PLT 5. There is no bubble in
4. Open the shield of the counting vial, observe
the RBC bath.
whether the bubble blending in the RBC bath is
4The RBC gain setting is
normal. If no bubble comes out, make treatment by
inappropriate.
referring to “No bubble comes out during bubble
6. Noise interference
5. Enter the “Service → Adjustment →CBC
Gain(Blood)” interface and readjust the RBC gain.
sources.
interface, execute the “Unclog” operation, check that
the Ruby Aperture is normal, and conduct the test
1. The hole of the WBC again.
WBC test
bath is blocked. 2. Enter the “Service → Mechanical Check” interface,
value of
14 2. Fault of negative execute the “Neg. Pressure Test” operation, and test
normal
pressure whether the negative pressure for testing is normal.
sample < 1
3. Fault of valve 3. Enter the “Service → Mechanical Check” interface
normally. Observe whether the liquid in the WBC bath
leaks during testing.
15 WBC test 1. The hole of the WBC 1. Enter the “Service → Daily Maintenance → Upkeep”
value of bath is blocked. interface, execute the “Unclog” operation, check that
normal 3. Fault of WBC the Ruby Aperture is normal, and conduct the test
sample > blending (no bubble, or again.
50 insufficient bubble, no 3. Open the shield of the counting chamber, execute
hemolysis) the testing sequence, and observe whether there is
2. Fault of 86H lyse (the bubble coming out in the WBC bath during the two
86H lyse is used up; bubble blending processes. If no, make treatment by
fault of SV12 valve) referring to “No bubble comes out during bubble
183
2. Check whether the 86H lyse is used up, whether the

valve SV12 works normally, and whether the
quantification of the 86H lyse syringe is accurate.
Warning: (1) In case of fault of the instrument, immediately contact the

agent to obtain technical support!
(2) Only professionals confirmed by the Company can repair this
instrument. For replacement of accessories, contact the
manufacturer or agent for supply.
184
Appendix I: Schematic Diagram of Fluidics
185

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Hemaray 86 Service Manual V1.0e

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Hemaray 86/89 Service Manual

Hemaray 86/89 Service Manual

Copyright: Rayto Life and Analytical Sciences Co., Ltd.

Main Alarm Icons Used in this Instrument

Biological and chemical hazards

High voltage warning

The operator should operate according to the

MAIN ALARM ICONS USED IN THIS INSTRUMENT.........................1

2.10.6 Installation of Automatic Sampler Driver Board Program...........................................34

CHAPTER 3 HARDWARE SYSTEM...............................................36

CHAPTER 4 SOFTWARE SYSTEM................................................80

CHAPTER 5 FLUIDICS SYSTEM....................................................83

CHAPTER 6 MECHANICAL SYSTEM...........................................108

CHAPTER 7 COMPONENT ADJUSTMENT AND DETECTING....144

CHAPTER 9 CLINICAL ALARM.....................................................154

CHAPTER 10 FAULTS AND TREATMENT...................................157

APPENDIX I: SCHEMATIC DIAGRAM OF FLUIDICS....................170

Figure 1- Front View of Instrument

1- Shell 2 - Sample compartment Door 3 - Automatic Sampler 4 - Tube Rack 5 - Liquid

Figure 1- Back View of Instrument

Figure 1- Axial Side View 1 of Instrument

Figure 1- Axial Side View 2 of Instrument

.3.2 Sample Suction

.3.4 Hemoglobin Concentration (HGB) Measurement

 Lymphocyte Percentage (LYM%)

 Lymphocyte Quantity (LYM#)

 Monocyte Quantity (MON#)

 Eosinophil Quantity (EOS#)

 Basophil Quantity (BAS#)

 Hemoglobin Concentration (HGB)

 Mean Corpuscular Hemoglobin (MCH)

 Mean Corpuscular Hemoglobin Concentration (MCHC)

 Red Cell Distribution Width - Coefficient of Variation (RDW-CV)

 Platelet Large Cell Ratio (P-LCR)

Table 1-2 Blank Test Requirements

Parameter Local Range

Table 1-3 Carry-over

Table 1-4 Repeatability

Parameter Whole Blood Mode

±3 (Absolute (25%-35%) and WBC ≥ 4 x

Table 1-5 Linearity

Parameter Whole Blood Mode

PLT ±8% or ±10 x /L 20-999 x /L

Caution: The accessories packed should be consistent with the packing

.8.2 Handling Method

.9 Installation and Use Environment

.10 Requirements of Power Supply

.11 Requirements of Space

.12 Removal of Auxiliary Fixing Structure for Transportation

.13 Connection of Reagent and Waste Liquid Containers

Table 2-6 Reagent Connector Connection Table

S/N Reagent Machine Connector

.14 Connecting Power Supply

.15 Preparation before Power-on

Table 2-7 Blank Indicators

Parameter Blank Value

2. CBC Gain Test

Table 2-8 Table of Requirements of Centers of Gravity of Fresh Blood

NEU ON FS 612 676

LYM ON FL 309 341

NEU ON SS 255 345

.17 Software Installation and Upgrade

5. Double-click the file to be programmed.

.17.3.2 Programming QuickLoader