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CARBOHYDRATES
CARBOHYDRATES
Chemical methods
o Oxidation reduction method
Alkaline copper reduction method
Folin wu method
Benedict’s method
Neocuproine method
Nelson Somogyi method
Alkaline ferric reduction method
Hagedorn Jensen method
o Condensation method
Dubowski method
Glucose methodologies
Chemical method
o Oxidation reduction method
Alkaline copper reduction method
Folin Wu method
o Cupric ion > cuprous ion > cuprous oxide + phosphomolybdic
acid > phosphomolybdous > blue
Benedict’s method
o Reducing sugar + benedict’s reagent then heated > reduction
reaction > brick red
o Fehling’s principle: Oxidized aldolases > carboxylic acid > cupric
ion + tartrate > cuprous oxide > brick red
Nelson somogyi method
o Reducing sugars heated with alkaline copper tartrate cupric ion
> cuprous oxide + arsenomolybdate > molybdic acid >
molybdenum blue
Neocuproine method
o Cuprous ion + neocuproine > cuprous-neocuproine complex +
chloroform methanol mixture > yellow or yellow orange
Alkaline ferric reduction method
Hagedorn Jensen method
o Reduction of yellow ferricyanide to colorless ferrocyanide
o Condensation method
Dubowski method
Condensation of glucose with primary aromatic amine > glycosylamine +
schiff’s base
Glucose + ortho-toluidine > green compound
Enzymatic method