The Feasibility of Eucalyptus Leaves and Pomelo Peelings Extract As Mosquito Repellent

Region 1
Alaminos City Division
ALAMINOS CITY NATIONAL HIGH SCHOOL
Alaminos City, Pangasinan
LIFE SCIENCE
School Level
The Feasibility of Eucalyptus (Eucalyptus

Tracy Mitch C. Capitle
globulus) leaves and Pomelo (Citrus
maxima)
Ian Lawrence peelings
Ranches extract as Mosquito
repellent April Rose Caballero
RESEARCHERS
Region I
ALAMINOS CITY DIVISION
ALAMINOS CITY NATIONAL HIGH SCHOOL
Alaminos City, Pangasinan
The Feasibility of Eucalyptus (Eucalyptus globulus) Leaves Extract and Pomelo
(Citrus maxima) peelings extract as Mosquito Repellent
In Partial Fulfilment of the Requirements in Research II
Life Science
Ian Lawrence Ranches
April Rose Caballero
Tracy Mitch Capitle
RESEARCHERS
Mr. Bernard Mendez
Project Adviser
4
RESEARCH PLAN
Name of Researchers: Ian Lawrence C. Ranches Category: Physical Science
April rose Caballero Level: School Level
Tracy Mitch C. Capitle
School: Alaminos City National High School
School Address: San Jose Drive, Poblacion, Alaminos City, Pangasinan
Title Project: The Feasibility of Eucalyptus (Eucalyptus globulus) leaves and Pomelo (Citrus
maxima) peelings extract as Mosquito repellant
A. RATIONALE
Mosquitoes and the diseases they spread have been responsible for killing more people than
all the wars in history. Even today, tens of millions more are killed and debilitated by a host of
other mosquito-borne diseases, including filariasis, yellow fever, malaria, and dengue.
Eucalyptus has a powerful smell that interferes with mosquito delicate senses and make it
difficult for them to locate their food sources. The oil from these trees also repels other insects such
as ticks, midges and sandflies. While the presence of the plant itself will work as a repellent in your
yard, the oil can be applied directly to the skin as well, but should be applied regularly for optimum
protection.
Mosquitos and ants especially detest the smell of orange and lemon peel. These citrus peels
contain organic chemicals that have the ability to repel mosquitos and other insects, citrus fruits are
used in different ways to help in the fight against mosquitos and if they are applied correctly, we
might stand a chance to win some of the fights.
5
B. STATEMENT OF THE PROBLEM
This study was conducted to determine the feasibility of Eucalyptus (Eucalyptus globulus) Leaves
and Pomelo (Citrus maxima) Peelings Extracts as Mosquito repellant.
Specifically, it aimed to answer the following questions:
1. Are Eucalyptus (Eucalyptus globulus) Leaves and Pomelo (Citrus maxima) Peelings Extract
contain mosquito repellent properties?
2. Is there a significant difference among the treatments of the Eucalyptus (Eucalyptus globulus)
leaves extract and Pomelo (Citrus maxima) peelings extract in terms of the number of
mosquitoes repelled per unit time?
3. Is there a significant difference between the Eucalyptus (Eucalyptus globulus) Leaves and the
Pomelo (Citrus maxima) Peelings Extract and the commercial one in terms of the number of
mosquitoes repelled per unit time and in terms of cost?
C. HYPOTHESIS OF THE STUDY
This was directed by the following hypotheses:
Null Hypotheses
1. The Eucalyptus (Eucalyptus globulus) Leaves and Pomelo (Citrus maxima) Peelings Extract
does not contain mosquito repellent properties.
6
2. There is no significant difference among the treatments of the Eucalyptus (Eucalyptus globulus)
mosquitoes repelled per unit time.
3. There is no significant difference between the Eucalyptus (Eucalyptus globulus) Leaves and the
mosquitoes repelled per unit time and in terms of cost.
D. OBJECTIVES OF THE STUDY
This study sought to determine the feasibility of Eucalyptus (Eucalyptus globulus) Leaves and
Pomelo (Citrus maxima) Peelings Extract as Mosquito repellant.
Specifically, this study aimed to achieve the following:
1. To determine if the Eucalyptus (Eucalyptus globulus) Leaves and Pomelo (Citrus maxima)
Peelings Extract contain mosquito repellent properties.
2. To determine if there is a significant difference among the treatments of the Eucalyptus
(Eucalyptus globulus) Leaves and Pomelo (Citrus maxima) Peelings Extract in terms of the
number of mosquitoes repelled per unit time.
3. To determine if there is a significant difference between the Eucalyptus (Eucalyptus globulus)
Leaves and the Pomelo (Citrus maxima) Peelings Extract and the commercial one in terms of the
number of mosquitoes repelled per unit time and in terms of cost.
7
RESEARCH METHOD
A. Collection of Raw Materials and Equipments
The raw material used in this study was Eucalyptus leaves extract and Pomelo peelings extract that
would be gathered by the researchers at Bued, Alaminos City Pangasinan. The gathered materials might
be mature enough to carry all the important chemical properties that can help in making mosquito
repellent and it should be clean before doing the experiment. Water would also be used to be able to
make perfect concentration.
Equipment’s/ Materials
The equipment’s and instrument that was used in conducting this study includes Erlenmeyer
flask which, Mortar and Pestle, Close container, Filter paper, Beaker, Gloves, Lab Gowns and
Mask.
Procedure
1. Gathering of Materials and Laboratory Equipment’s
Three hundred (300) grams of extracted Eucalyptus leaves and Three hundred (300) grams of
extracted Pomelo peelings that was gathered from Bued, Alaminos City Pangasinan. The said
equipment’s was borrowed at the laboratory of Alaminos City National High School (ACNHS).
2. Safety Precautions
During the whole experimentation, some safety clothing was been dressed by the researchers in
order to avoid some hazardous chemical that was in the laboratory. This safety clothing includes
Gloves, Laboratory Gown, Mask.
8
3. Phytochemical Analysis
The Eucalyptus leaves extract and Pomelo peelings extract was brought to Virgen Milagrosa
University Foundation- College of Pharmacy for photochemical testing. It is a method of screening that
could help identify the chemicals that have anti-mosquito property. This could help in identifying
whether the Eucalyptus leaves extract and Pomelo peelings was classified in this.
4. Preparation of Materials
The plant extract was obtained by maceration method. Three hundred (300) grams of extracted
Eucalyptus leaves and extracted Pomelo peelings were collected by the premises of one of the
researchers. The collected plant parts were weighted, cut, blended, and crashed.
5. Preparation of Treatments
Using the beaker, the amount of Papaya leaf extract and Lemongrass oil was measured
separately for each treatment.
6. Pre-Treatment Evaluation
The mosquitoes were observed by the researchers to identify the difference between the
commercial mosquito repellant and the alternative mosquito repellant. This procedure should be applied
before the application of treatments. The mosquitoes were observed inside the laboratory with the help
of medical technologists and health expert. The results of this evaluation were recorded and used to
identify the difference between the commercial and Eucalyptus leaves extract and Pomelo peelings
extract.
9
7. Application of treatment
The different prepared treatments were applied to the observed mosquitoes. To avoid bias in
applying the treatments, the mosquitoes should use same Eucalyptus leaves extract and Pomelo peelings
extract before the application of treatment. This could help he researcher to determine the difference
between commercial mosquito repellent and Eucalyptus leaves extract and Pomelo peelings extract.
8.Post-Treatment Evaluation
After the application of treatment, the difference before and after the Papaya leaf extract and
Lemongrass oil was applied and observed by the researchers. The result of this evaluation was important
in finalizing the difference of this extract and oil from the commercial one.
9.Disposal of Treatment
The mosquitoes that were used in this study were properly disposed by placing them in the
plastic bag and then was buried underground so that it will not cause any harm to human and
environment.
10. Statistical Analysis
One-way ANOVA was used to test the independent variable. The level of significance that was
used is 0.05. If the calculated value is greater than the critical value then the null hypothesis will be
rejected. If the calculated value is lesser than the critical value then the null hypothesis will be accepted.
10
ABSTRACT
Title : The Feasibility of Eucalyptus (Eucalyptus globulus) leaves and Pomelo (Citrus maxima)
peelings extract as Mosquito repellant
Researcher : April Rose Caballero, Tracy Mitch Capitle and Ian Lawrence Ranches
Year Level : G9 – Dalton
Adviser : Mr. Bernard Mendez
School : Alaminos City National High School
Mosquitoes are carriers, or vectors, for some of humanity's most deadly illnesses, and they are
public enemy number one in fight against global infectious disease. Mosquito- borne diseases cause
millions of deaths worldwide every year with a disproportion are effect on children and the elderly in
developing countries. This study was conducted to (1) Determine if the Eucalyptus Leaves and Pomelo
Peelings Extract contains mosquito repellent properties (2) Determine if there is a significant difference
among the treatments of the Eucalyptus Leaves and Pomelo Peelings Extract in terms of the number of
mosquito repelled per unit time (3) Determine if there is a significant difference between the Eucalyptus
Leaves and the Pomelo Peelings Extract and the commercial one in terms of the number of mosquito
repelled per unit time and in terms of cost.
Three hundred (300) grams of Eucalyptus leaves and Pomelo peelings were acquired from
Alaminos City, Pangasinan. They were extracted and prepared using the following treatments in 3
replicates. T0, negative control (placebo); T1 (75 ml of Eucalyptus leaves extract and 25 ml of
Pomelo peelings extract) T2 (25 ml of Eucalyptus leaves extract and 75 ml of Pomelo peelings
extract) T3 (50 ml of Eucalyptus leaves extract and 50 ml of Pomelo peelings extract) T4
(commercial: Baygon).
The data gathered based from the experiment were correlated with one another to find
significant differences.
11
Results revealed that: (1) Eucalyptus leaves and Pomelo peelings extract contains mosquito repellent
properties. (2) There is a significant difference among the different treatment in terms of the number of
mosquitoes repelled per unit time. (3) There is a significant difference between the Eucalyptus leaves
and Pomelo peelings extract and the commercial one (baygon).
Furthermore, the study contributes to the society. The result of the study showed that
Eucalyptus leaves and Pomelo peelings extract is effective as mosquito repellent.
12
ACKNOWLEDGEMENT
This study took more than months to finish than it would have been.
First and foremost, the researchers would like to thank God for the wisdom and strength he has
given to us all throughout the study.
To the researcher’s family, a deepest gratitude is given for their unending love, care and support,
mentally and financially;
To Mr. Bernard Mendez, the research teacher and the research adviser for her assistance in the
computations and improvement of this study;
To Mr. Jose Ramil Sibun, Science Head Teacher, for extending his help and technical assistance
to the project;
To Mrs. Vivian R. Camba, our chemistry teacher, for the help an assistance in terms of chemical
analysis to this study;
To Mr. Orlando Guerrero, our Principal, for the support;
To the VMUF College of Pharmacy Laboratory for the honored access and assistance in our
study.
Sincerest appreciation is extended to their, friends and classmates for sharing their ideas,
opinions, suggestions regarding the study.
The Researchers
Tracy Mitch Capitle
13
Dedication
I dedicate this to everyone who believes in us and supports us. And to our Almighty God who is giving
us strength and knowledge to continue our study.
The Researchers,
Tracy Mitch Capitle
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The Feasibility of Eucalyptus
(Eucalyptus globulus) leaves
and Pomelo (Citrus maxima)
peelings extract as Mosquito
repellant
In Partial Fulfillment for the Requirements

In Research Practicum and Technical Writing
June 2021

Tracy Mitch Capitle
Researchers
Bernard G. Mendez
Research Adviser
3
The feasibility of Eucalyptus (Eucalyptus globulus) leaves and Pomelo (Citrus maxima) peelings extract as
Mosquito repellant
TABLE OF CONTENTS
TITLE PAGE …………………………………………… i

RESEARCH PLAN …………………………………………… ix
ACKNOWLEDGEMENT ………………………................................. xvii
ABSTRACT …………………………………………… xviii
INTRODUCTION …………………………………………… 2
Background of the Study ……………………………………….. 2
Statement of the Hypothesis…………………………………….. 3
Statement of the Problem……………………………………….. 4
Objectives of the Study ………………………………………... 4
Significance of the Study……………………………………….. 5
Scope and Limitation ………………………………………… 5
Definition of Terms……………………………………………… 6
METHODOLOGY…………………………………………………. 8
Materials …………………………………………… 8
Collection of Material …………………………………………… 8
Preparation of Materials …………………………………………… 9
Preparation of Treatments………………………………..………….. 10
Pre-treatment Evaluation ……………………………………………. 10
Application of Treatments…………………………………………… 11
Post-treatment Evaluation ……………………………………………. 11
Statistical Analysis ……………………………………………. 12
RESULTS AND DISCUSSIONS…………………………………… 17
SUMMARY, CONCLUSION AND RECOMMENDATION……... 17
Summary ……………………………………………. 17
Conclusion …………………………………………….. 17
Recommendation …………………………………………….. 17
APPENDICES …………………………………………….. 19
BIBLIOGRAPHY …………………………………………….. 26
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Mosquito repellant
CHAPTER I
INTRODUCTION
A. Background of the study
Almost everyone has had the unpleasant experience of being bitten by a mosquito. Mosquito
bites can cause skin irritation through an allergic reaction to the mosquito’s saliva – this is what causes
the red bump and itching. But a more serious consequence of some mosquito bites may be transmission
of serious diseases and viruses such as Malaria, Dengue virus, Zika and West Nile virus. Mosquitoes
and the diseases they spread have been responsible for killing more people than all the wars in history.
Even today, tens of millions more are killed and debilitated by a host of other mosquito-borne diseases,
including filariasis, yellow fever, malaria, and dengue.
Dengue is a mosquito-borne viral disease that has rapidly spread in all regions in the recent
years. Dengue virus is transmitted by female mosquitoes mainly of the species Aedes aegypti. Dengue is
widespread throughout the tropics, with local variations in risk influenced by rainfall, temperature,
relative humidity and unplanned rapid urbanization.
Dengue viruses are spread to people through the bite of an infected Aedes species mosquito.
Dengue is common in more than 100 countries around the world. Forty percent of the world’s
population, about 3 billion people, live in areas with a risk of dengue. Dengue is often a leading cause of
illness in areas with risk.
Although less common, some people develop severe dengue, which can be any number of
complications associated with severe bleeding, organ impairment and/or plasma leakage. Severe dengue
has a higher risk of death when not managed appropriately. Severe dengue was first recognized in the
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Mosquito repellant
1950s during dengue epidemics in the Philippines and Thailand. Today, severe dengue affects most
Asian and Latin American countries and has become a leading cause of hospitalization and death among
children and adults in these regions.
According to the World Health Organization (WHO), dengue is now endemic in over 100
countries. As many as 3.6 billion people, or 40% of the world’s population, reside in dengue-endemic
areas. Each year, an estimated 400 million people are infected with dengue virus, 100 million become ill
with dengue, and 21,000 deaths are attributed to dengue. In 2021 itself, as of February 20, 2021, the
Philippines reported 10 260 dengue cases, including 32 deaths. The number of cases is 72% lower
compared to the 37 487 cases that were reported in the same period in 2020.
Eucalyptus has a powerful smell that interferes with mosquito delicate senses and make it
difficult for them to locate their food sources. The oil from these trees also repels other insects such as
ticks, midges and sandflies. While the presence of the plant itself will work as a repellent in your yard,
the oil can be applied directly to the skin as well, but should be applied regularly for optimum
protection.
Mosquitos and ants especially detest the smell of orange and lemon peel. These citrus peels
contain organic chemicals that have the ability to repel mosquitos and other insects, citrus fruits are used
in different ways to help in the fight against mosquitos and if they are applied correctly, we might stand
a chance to win some of the fights.
A. STATEMENT OF THE PROBLEM
This study was conducted to determine the feasibility of Eucalyptus (Eucalyptus globulus) Leaves
and Pomelo (Citrus maxima) Peelings Extracts as Mosquito repellant.
6
Mosquito repellant
Specifically, it aimed to answer the following questions:
4. Are Eucalyptus (Eucalyptus globulus) Leaves and Pomelo (Citrus maxima) Peelings Extract
contain mosquito repellent properties?
5. Is there a significant difference among the treatments of the Eucalyptus (Eucalyptus globulus)
mosquitoes repelled per unit time?
6. Is there a significant difference between the Eucalyptus (Eucalyptus globulus) Leaves and the
mosquitoes repelled per unit time and in terms of cost?
B. HYPOTHESIS OF THE STUDY
This was directed by the following hypotheses:
Null Hypotheses
does not contain mosquito repellent properties.
5. There is no significant difference among the treatments of the Eucalyptus (Eucalyptus globulus)
7
Mosquito repellant
6. There is no significant difference between the Eucalyptus (Eucalyptus globulus) Leaves and the
Alternative Hypotheses
contains mosquito repellent properties.
2. There is a significant difference among the treatments of the Eucalyptus (Eucalyptus globulus)
3. There is a significant difference between the Eucalyptus (Eucalyptus globulus) Leaves and the
C. OBJECTIVES OF THE STUDY
This study sought to determine the feasibility of Eucalyptus (Eucalyptus globulus) Leaves and
Pomelo (Citrus maxima) Peelings Extract as Mosquito repellant.
Specifically, this study aimed to achieve the following:
4. To determine if the Eucalyptus (Eucalyptus globulus) Leaves and Pomelo (Citrus maxima)
Peelings Extract contain mosquito repellent properties.
8
Mosquito repellant
5. To determine if there is a significant difference among the treatments of the Eucalyptus
(Eucalyptus globulus) Leaves and Pomelo (Citrus maxima) Peelings Extract in terms of the
number of mosquitoes repelled per unit time.
6. To determine if there is a significant difference between the Eucalyptus (Eucalyptus globulus)
Leaves and the Pomelo (Citrus maxima) Peelings Extract and the commercial one in terms of the
number of mosquitoes repelled per unit time and in terms of cost.
D. SIGNIFICANCE OF THE STUDY
This study, the feasibility of Eucalyptus (Eucalyptus globulus) Leaves and Pomelo (Citrus
maxima) Peelings Extract as Mosquito repellant will benefit the following:
Community. This research can help in finding economical ways to make an organic Mosquito repellant
for the people in our community.
Government agencies. This will serve as a guide to government agencies in making an organic
Mosquito repellant.
Drug institutions and manufactures. This study will help the drugs institutions and manufactures to
produce an organic Mosquito repellant naturally and economically.
Students. This study will encourage students to conduct related studies that will help the society and the
community.
Researchers. This will guide the researchers in gaining more knowledge and will be encouraged to
conduct further study regarding Mosquitoes.
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Mosquito repellant
E. SCOPE AND LIMITATIONS
This study will focus on, the feasibility of Eucalyptus (Eucalyptus globulus) Leaves and Pomelo
(Citrus maxima) Peelings Extract as Mosquito Repellent. The Eucalyptus leaves and the Pomelo
peelings extract were only used in the study. The Eucalyptus leaves and Pomelo peelings extract were
extracted in the school laboratory of Alaminos City National High School. Four (4) treatments with
specified proportions were the following. T0 (placebo), T1 (75 ml of Eucalyptus leaves extract and 25
ml of Pomelo peelings extract), T2 (25 ml of Eucalyptus leaves extract and 75 ml of Pomelo peelings
extract), T3 (50 ml of Eucalyptus leaves extract and 50 ml of Pomelo peelings extract), T4 (commercial
product; Baygon).
RESEARCH PARADIGM
Independent Variable Dependent Variable
1. Levels of Concentration of the

eucalyptus leaves (eucalyptus globulus)
and Pomelo (Citrus maxima) peelings
extract The Feasibility of Eucalyptus
A. 95% B. 75% C. 50% D. 25% (Eucalyptus globulus) leaves and
Pomelo (Citrus maxima) peelings
3. Positive control
extract as Mosquito repellant
Commercial Product
4. Negative Control
Placebo
i. Time
ii. Temperature
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Mosquito repellant
GANTT CHART
Januar
February March April May June July August
y
Conceptualization
of the Research
be undertaken
Finding some
information in
Library
Preparation of
the Research
Proposal and
approval
Planning the
experimental
design
Data Gathering
and Data
Analysis
Phytochemical
Screening test
Submission of
Chapter I and
Chapter II
Submission of
Chapter III
Submission of
Chapter IV
Bibliography
Appendices
Presentation of
the Results
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Mosquito repellant
COST ANALYSIS
MATERIALS COST
EUCALYPTUS LEAVES ₱0.00
POMELO PEELINGS ₱0.00
TRANSPORTATION ₱750.00
TEST MOSQUITOES ₱500.00
PHYTOCHEMICAL SCREENING ₱500.00
TOTAL ₱1750.00
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Mosquito repellant
CHAPTER II
METHODOLOGY
Research Design
This study used Complete Randomized Design (CDR) to provide observation on the
acceptability test of the product. Respondent will be selected from the different section in order to create
common difference among them. To avoid bias in the testing respondents will be equally distributive on
the prepared blocks.
A. Collection of Raw Materials and Equipment’s
The raw material used in this study was Eucalyptus leaves extract and Pomelo peelings extract
that would be gathered by the researchers at Bued, Alaminos City Pangasinan. The gathered materials
might be mature enough to carry all the important chemical properties that can help in making mosquito
repellent and it should be clean before doing the experiment. Water would also be used to be able to
make perfect concentration.
Equipment’s/ Materials
The equipment’s and instrument that was used in conducting this study includes Erlenmeyer
flask which, Mortar and Pestle, Close container, Filter paper, Beaker, Gloves, Lab Gowns and
Mask.
B. Procedure
1. Gathering of Materials and Laboratory Equipment’s
13
Mosquito repellant
Three hundred (300) grams of extracted Eucalyptus leaves and Three hundred (300) grams of
extracted Pomelo peelings that was gathered from Bued, Alaminos City Pangasinan. The said
equipment’s was borrowed at the laboratory of Alaminos City National High School (ACNHS).
2. Safety Precautions
During the whole experimentation, some safety clothing was been dressed by the researchers in
order to avoid some hazardous chemical that was in the laboratory. This safety clothing includes
Gloves, Laboratory Gown, Mask.
3. Phytochemical Analysis
The Eucalyptus leaves extract and Pomelo peelings extract was brought to Virgen Milagrosa
University Foundation- College of Pharmacy for photochemical testing. It is a method of screening that
could help identify the chemicals that have anti-mosquito property. This could help in identifying
whether the Eucalyptus leaves extract and Pomelo peelings was classified in this.
4. Preparation of Materials
The plant extract was obtained by maceration method. Three hundred (300) grams of extracted
Eucalyptus leaves and extracted Pomelo peelings were collected by the premises of one of the
researchers. The collected plant parts were weighted, cut, blended, and crashed.
5. Preparation of Treatments
Using the beaker, the amount of Papaya leaf extract and Lemongrass oil was measured
separately for each treatment.
14
Mosquito repellant
Treatments Amount of Pomelo peelings Amount of eucalyptus leaves
extract (ml) extract (ml)
T0 0 0
T1 75 25
T2 25 75
T3 50 50
T4 Commercial insect repellent Commercial insect repellent
8. Pre-Treatment Evaluation
The mosquitoes were observed by the researchers to identify the difference between the
commercial mosquito repellant and the alternative mosquito repellant. This procedure should be applied
before the application of treatments. The mosquitoes were observed inside the laboratory with the help
of medical technologists and health expert. The results of this evaluation were recorded and used to
identify the difference between the commercial and Eucalyptus leaves extract and Pomelo peelings
extract.
9. Application of treatment
The different prepared treatments were applied to the observed mosquitoes. To avoid bias in
applying the treatments, the mosquitoes should use same Eucalyptus leaves extract and Pomelo peelings
extract before the application of treatment. This could help he researcher to determine the difference
between commercial mosquito repellent and Eucalyptus leaves extract and Pomelo peelings extract.
10.Post-Treatment Evaluation
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Mosquito repellant
After the application of treatment, the difference before and after the Papaya leaf extract and
Lemongrass oil was applied and observed by the researchers. The result of this evaluation was important
in finalizing the difference of this extract and oil from the commercial one.
11.Disposal of Treatment
The mosquitoes that were used in this study were properly disposed by placing them in the
plastic bag and then was buried underground so that it will not cause any harm to human and
environment.
12. Statistical Analysis
One-way ANOVA was used to test the independent variable. The level of significance that was
used is 0.05. If the calculated value is greater than the critical value then the null hypothesis will be
rejected. If the calculated value is lesser than the critical value then the null hypothesis will be accepted.
16
Mosquito repellant
Gathering of Raw
Phytochemical Testing
Materials and Safety Pre-Cautions
Laboratory Equipments (Phytochemical
(Wear some safety
screening that would
(300 grams of clothing like gloves,
help identify the
Eucalyptus leaves and laboratory gown and
chemicals that can
300 grams of Pomelo mask)
repellent mosquitoes)
peelings was collected)
Preparation Raw
Materials and
Equipments
(The plants would be
Pre-Treatment Preparation of cleaned with tap wazter
Evaluation Treatments and stored at room
temperature. The
equipments could be
borrowed at the
laboratory of the school)
Statistical Analysis
Application of (The data gathered were
Treatments Post-Treatment
Evaluation analyzed using One Way
Anova to determine if
this study is feasible)
Flow Chart
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18
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CHAPTER III
RESULTS AND DISCUSSIONS
This portion of the study shows the data gathered, detail of the analysis, and interpretation of the
findings based on the problems that was stated.
In this chapter, it tries to discuss the observation noted on the experiment and the characteristics
of the mosquito repellent made from Eucalyptus leaves and Pomelo peelings extracts. Four treatments
were prepared in order to test the feasibility of Eucalyptus leaves and Pomelo peelings extracts as
mosquito repellent.
Table 1a: ANOVA TABLE
Trials Population density of mosquitoes
T0 T1 T2 T3 T4
1 0 6 5 7 7
2 0 3 4 9 8
3 0 4 7 8 6
N 0 13 16 24 21
Mean 0 4.33 5.33 8 7
Legend: T0 – placebo
To determine if there were significant difference in the number of drove away mosquitoes of the
different treatments; ANOVA was employed. If the F-value is greater than tabular value, then there is a
significant difference. Table 1b shows the F-value of 4.27 is greater than the tabular value of 3.48
indicating significant difference in the pH values of the treatment. This further means that the pH values
differ greatly from each other.
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Mosquito repellant
Source of Sum of Degrees of Mean F-value Tabular Interpretation
variation Squares Freedom
Between 17.22 4 4.31 4.27 3.48 Significant
Within 10.12 10 1.01
Total 27.34
Table 1b: ANOVA table as to pH
To determine where the significant difference in the population of mosquitoes, a further
statistical analysis was done using the Scheffe’s Test. A Scheffe’s value that is higher to the product of
df and the tabular value indicates a significant difference.
Table 1c: Multiple Comparisons using Scheffe’s Test
Between Treatments Ft Scheffe’s critical Interpretation
value
T0 and T1 27.99 13.92 Significant
T1 and T2 1.49 13.92 Not significant
T1and T3 20.10 13.92 Significant
T1 and T4 10.64 13.92 Not Significant
T2and T3 10.64 13.92 Not Significant
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Mosquito repellant
T2and T4 4.16 13.92 Not significant
T3and T4 1.49 13.92 Not significant
The above table shows that there is significant difference in the number of mosquitoes drove
away between different concentrations of Eucalyptus leaves and Pomelo peelings extracts and the
commercial mosquito repellent. However, treatment 1 and treatment 2, 1 and 4, 2 and 3, 2 and 4, 3 and 4
do not significantly differ in their efficacy meaning any of them have same efficacy to the number of
mosquitoes drove away.
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Mosquito repellant
CHAPTER IV
SUMMARY, CONCLUSION, RECOMMENDATION
SUMMARY
It was found that the treatment of Eucalyptus leaves and Pomelo peelings extract and the
commercial one which is the Baygon are almost alike in effectiveness as mosquito repellent.
CONCLUSION
Based from the result of the study, the researcher came up with the following conclusion:
1. Eucalyptus leaves extract and Pomelo peelings extract is effective as mosquito repellent.
2. There is a significant difference among the different treatment in terms of effectiveness.
3. There a significant difference between the Eucalyptus Leaves and the Pomelo Peelings Extract and
the commercial one in terms of texture and aroma.
4. There is a significant difference between the Eucalyptus leaves extract and Pomelo peelings
extract and the commercial one (baygon).
RECOMMENDATION
For those who would like to go deeper into the study, the researchers would like to
recommend that:
1.Make use of other plants that might be effective to make mosquito repellent.
2. Improve this study.
3. Consider using different statistical tools to support the significance of the study.
22
Mosquito repellant
BIBLIOGRAPHY
Online Sources
National Geographics (April 11, 2010). Mosquitoes. Retrieved from
https://api.nationalgeographics.com/distributions/public/amp/animals/invertebrates/faxts/
mosquitoes
World Health Organization (March 2, 2020). Vector-borne diseases. Retrieved from
https://www.who.int/news-room/facts-sheets/details/vector-borne-diseases
S. Dayaram, Philippines declares nation alert after 456 die from dengue fever, CNN.
https://www.cnn.com/2019/07/16/health/philippines-dengue-national-alert-hnk-intl/
index.html
F. Baldacchino et al. (2013). The repellency of lemongrass oil against stable flies, tested
using video tracking. EDP Sciences.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3718533/
R. Rahayu et al. (2020). Potency of Papaya Leaf (Carica papaya L.) as Toxicant and
Repellent Against German Cockroach (Blattella germanica L.). Pakistan Journal of
Biological Sciences. https://scialert.net/fulltext/?doi=pjbs.2020.126.131
23

The Feasibility of Eucalyptus Leaves and Pomelo Peelings Extract As Mosquito Repellent

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The Feasibility of Eucalyptus Leaves and Pomelo Peelings Extract As Mosquito Repellent

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Region 1

Alaminos City Division

ALAMINOS CITY NATIONAL HIGH SCHOOL

Alaminos City, Pangasinan

The Feasibility of Eucalyptus (Eucalyptus

ALAMINOS CITY DIVISION

ALAMINOS CITY NATIONAL HIGH SCHOOL

Alaminos City, Pangasinan

The Feasibility of Eucalyptus (Eucalyptus globulus) Leaves Extract and Pomelo

(Citrus maxima) peelings extract as Mosquito Repellent

In Partial Fulfilment of the Requirements in Research II

Ian Lawrence Ranches

April Rose Caballero

Tracy Mitch Capitle

Mr. Bernard Mendez

Name of Researchers: Ian Lawrence C. Ranches Category: Physical Science

April rose Caballero Level: School Level

Tracy Mitch C. Capitle

School: Alaminos City National High School

School Address: San Jose Drive, Poblacion, Alaminos City, Pangasinan

might stand a chance to win some of the fights.

and Pomelo (Citrus maxima) Peelings Extracts as Mosquito repellant.

Specifically, it aimed to answer the following questions:

contain mosquito repellent properties?

mosquitoes repelled per unit time?

mosquitoes repelled per unit time and in terms of cost?

C. HYPOTHESIS OF THE STUDY

This was directed by the following hypotheses:

does not contain mosquito repellent properties.

mosquitoes repelled per unit time.

mosquitoes repelled per unit time and in terms of cost.

D. OBJECTIVES OF THE STUDY

Pomelo (Citrus maxima) Peelings Extract as Mosquito repellant.

Specifically, this study aimed to achieve the following:

Peelings Extract contain mosquito repellent properties.

2. To determine if there is a significant difference among the treatments of the Eucalyptus

number of mosquitoes repelled per unit time.

3. To determine if there is a significant difference between the Eucalyptus (Eucalyptus globulus)

number of mosquitoes repelled per unit time and in terms of cost.

A. Collection of Raw Materials and Equipments

make perfect concentration.

1. Gathering of Materials and Laboratory Equipment’s

Gloves, Laboratory Gown, Mask.

separately for each treatment.

10. Statistical Analysis

repelled per unit time and in terms of cost.

extract) T3 (50 ml of Eucalyptus leaves extract and 50 ml of Pomelo peelings extract) T4

and Pomelo peelings extract and the commercial one (baygon).

Eucalyptus leaves and Pomelo peelings extract is effective as mosquito repellent.

given to us all throughout the study.

mentally and financially;

computations and improvement of this study;

analysis to this study;

To Mr. Orlando Guerrero, our Principal, for the support;

opinions, suggestions regarding the study.

us strength and knowledge to continue our study.

In Partial Fulfillment for the Requirements

April Rose Caballero

TITLE PAGE …………………………………………… i

A. Background of the study

including filariasis, yellow fever, malaria, and dengue.

relative humidity and unplanned rapid urbanization.