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General Biology 1
QUARTER 3

ACTIVITY SHEETS
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General Biology 1-Grade 11
Learning Activity Sheets
Quarter 3

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 GENERAL BIOLOGY 1
QUARTER 3 – WEEK 7

NAME: _________________________________ GRADE & SECTION:

____________

LEARNING ACTIVITY SHEET No. 12 DATE ANSWERED:

LESSON 1
ENZYMES

Learning Competency:
 Describe the components of an enzyme. (STEM_BIO11/12-Ii-j-17)

Let’s Kick it off!

ACTIVITY 1: Fill the Gap

Directions: Fill in the gaps in the following sentences using the words in the
box below. Write your answer on the space provided for.

1. Enzymes are biological ____________that speed up chemical reactions

in
living organisms.
2. Enzymes are protein molecules, which are made up of long chains of
____________________.
3. The sequence and type of amino acids are _____________ in each
protein, so they produce enzymes with many different shapes and
functions.
4. The shape of an enzyme is very important to its ________________.
5. Enzymes hold ________________together to and in the correct
orientation.

different catalysts function molecules amino acids

64
 Are you taking it?

ACTIVITY 2.1: Lock and Key

Directions: Label the image below with the following terms. Write your
answer on the space provided after each number.

2. ____________

____________

+
3. ____________

ACTIVITY 2.2: Essay

Directions: Explain what would happen if a substrate molecule with a

different shape comes in contact with the enzyme’s active site. Write your
answer on the space provided below. (2pts)

___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

The active site and functional groups of its amino acids may lower activation
energy by: acting as a template for substrate orientation, stressing the
Background
substrates and stabilizing the transition state, providing a favorable micro
Enzyme
environment, and classes:
participating directly in the catalytic reaction.
Enzymes are biological catalysts. They are compounds that facilitate
chemical
Class reactions. The orderly and
Reaction course ofDescription
substratemetabolic processes is only possible
specificity
because each cellcatalyze
oxidoreductase is equipped with of
the transfer itsreducing
own genetically
equivalentsdetermined set of
from one redox
enzymes.
Enzymes
s It is
are only this
catalysts. that allows
Catalysts are coordinated
compounds
system to another. sequences
that accelerateof areactions
reaction
(metabolic
without beingpathways).
changed. Enzymes
Enzymes are not
are alsodestroyed
involvedor in many but
changed, regulatory
rather
catalyze the transfer of other groups from one molecule to
mechanisms
reused that
in the same allow the
chemicalmetabolism to adapt
reaction overand and to changing conditions.
over. Thegenerally
compounds Almost
that
transferases another. Oxidoreductases transferases require
all enzymes
enzymes act are
uponproteins.
are knownThe as
name of the enzyme
substrates. Enzymes usually
bindends
to aninactive
–ase site
and in
is
coenzyme 65
derived
the from the
substrate substrate
andare
lower that is affected by the
it. reaction
For example, enzymes that
alsothe energy
involved inneeded for
group transfer, to occur
but the acceptor making
is alwaysit
breakhydrolases
faster. down
The proteins are calledfor
energy required proteases.
a chemical reaction to occur is known as the
a water molecule
activation energy. The substrates change to form a product. The action of
often also referred to as “synthases”;
enzymes is usually very specific. This applies not only to the type of reaction
 SUBSTRATE
Your turn to lock and Key
ACTIVITY 3: ENZYME ENZYME-SUBSTRATE COMPLEX

Directions: Fill in the correct term involved in the lock and key model of
enzymatic action. Write your answer inside the box.

3. ___________
PRODUCT REGENERATED ENZYME

Now Do it!
2. ___________ 5.__________

4._____________

1.__________

Ace it! brainly.ph

ASSESSMENT

Directions: Matching Type. Match the following statement with their

definition given below. Write the letter of the chosen answer on the space
provided each number.

a. Product b. Active site c. Catalyst d. Substrate e. Activation

energy

_____1. amount of energy required for a chemical reaction to occur

_____2. substances that bring about a chemical reaction without being
changed itself
_____3. substance that an enzyme act upon
_____4. regions on surfaces of enzymes that fit the substrate
_____5. substance formed from the substrate at the end of a chemical
reaction with an enzyme
Directions: Multiple Choice. Write the letter of the correct answer. Write
your answer on the space provided before each number

_____1. Which of the statements regarding enzymes is false?

a.Enzymes are specific

b.Enzyme activity can be regulated.
c.Enzymes provide activation energy for reactions.
d.Enzymes are proteins that function as catalysts.
______2. Therelationship between an enzyme and a substrate molecule can best be
described as:
a. a temporary association.
b. a permanent mutual alteration of structure

66
 c. an association stabilized by a covalent bond.
d. one in which the enzyme is changed permanently.
*For items 3-5 refer to the diagram below:

_____3.Which letter represents the enzyme

a. A c. C
b. B d. None of the Above
_____4.Which letter represents the substrate?
a. A c. C
b. B d. None of the Above
_____5. Which model represents the "Lock & Key" model of enzyme action?
a.Model 1 c. Both model 1 and 2
b.Model 2 d. Neither model 1 and 2

BIBLIOGRAPHY
Belardo, G. M., et. al. (2016). General Biology 1 Textbook for Senior High School.
Vibal Group. 31-40.
Capiña, Mildred M. (2020). General Biology 1 – Grade 11 First Edition
.Alternative Delivery Mode. Quarter 1 – Module 1: Enzyme
Dela Cruz, S. B. (2003). Biology. Phoenix Publishing House, Inc. 88-91.
Desalle, Rob Et Al. (2008) Biology Texas Instruments Incorporated,p.33
The Commission on Higher Education and Philippine Normal University. Teaching
Guide for Senior High School-General Biology 1. Quezon City, Philippines:
Commission on Higher Education, 2006.
Raven, Johnson Et. Al., Biology: 9th Edition.

Online Source/s:
CPALMS, “Enzymes: The Greatest Biological Catalyst,” Accessed May 15, 2020.
https://www.cpalms.org/Public/PreviewResourceLesson/Preview/129074

https://www.anderson.net

brainly.ph.com

67
 GENERAL BIOLOGY 1
QUARTER 3 – WEEK 7

NAME: _________________________________ GRADE & SECTION:

____________
LEARNING ACTIVITY SHEET No. 13 DATE ANSWERED:

LESSON 2 Factors Affecting Enzymatic

Activity

Learning Competency:
 Determine how factors such as pH, temperature, and substrate affect
enzymatic activity. (STEM_BIO11/12-Ii-j-19)

Let’s Kick it off!

ACTIVITY 1: Review it Out!

Directions: Using the Hints provided for each item, fill the missing letters to
complete the word/phrase.

ACTIVE SITE 1. part of the substrate where the enzyme bind

E N Z Y M E – S U B S T R A T E 2. forms when an enzyme acts on a specific

COMPLEX
substrate

SUBSTRATE 3. reactant or molecule that enzymes catalyzes

A C T I V A T I O N E N E R G Y 4. energy required for a chemical reaction to occur

CO ENZYME 5. enhances the action of an enzyme by initiating

or aiding enzyme to function

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Are you taking it?

ACTIVITY 2: Concept Map

Directions: What are the factors affecting enzymatic activities? Complete
the concept map below by writing each in the outer concept bubbles. You
can refer to the concept box below.

2. pH (Hydrogen Ion Concentration) can affect enzymatic activities by slowing and

hastening the reaction rate. Optimum temperature for most enzymatic activity is at
normal temperature. Increase in temperature from the optimum can at some point
hasten the enzymatic activity but beyond the enzyme’s threshold temperature will
As atocatalyst
result in a chemical
denaturation. reaction,
Denaturation 2 criteria
is the breaking should be met,
of bonds which to
resulting arelooser,
the major
more
characteristics of enzymes:
random structure of the molecule.
3. An1.increase
It should in speed
Substrate Concentration affects enzymatic activity by hastening the
up reactions.
rate
2. until such not
It should timebethat
usedsaturation of allpermanently
up or altered active sites because
by substrate
of theisreactions.
attained. By this
time, steady enzymatic activity rate will be achieved.
These catalysts
4. Enzyme only react
concentration with specificity
increment can result andtoonly produces
faster reactiona single product
rate as there from
is an
a reaction. Some enzymes only react for a particular substrate. Others,
increase of active sites for substrates to bind (provided that substrates are availablefrom a particular
couple andofenough).
substrates while some can also react from a range of molecules so long as it
contains the specific
5. Effects bonds or chemical
of Activators- group that
some enzymes the enzyme
require targets.
activators—co-factors, additional
molecules also referred to as prosthetic group (in case of allosteric enzymes, allosteric
Factors Affecting Enzymatic Activity
modulators or effectors are needed) for it to be active.
1.6. Temperature
Inhibitors inhibit enzymatic
can affect activity.
enzymatic An increase
activities in inhibitor
by slowing and concentration
hastening the can hinder
reaction
enzyme
rate. rate reaction
Optimum depending
temperature for moston enzymatic
the nature activity
of the inhibitors.
is at normalInhibitors can be
temperature.
classified
Increase in as competitive,from
temperature non-competitive,
the optimum and canuncompetitive.
at some point hasten the enzymatic
7. activity
Accumulation of the end-products can affect
but beyond the enzyme’s threshold temperature rate of reaction
will when
result produced products
to denaturation.
crowd the active
Denaturation is thesites, lessening
breaking the chance
of bonds resultingfortosubstrates
looser, moreto bind there.structure of the
random
8. molecule.
Effects of light/radiation some enzymes are sensitive to light or radiation. Some even69
require light/radiation for enzymatic activity to start.
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9. Water may and can be one of the reactants in chemical reactions. It can limit or even
suppress enzyme activity of cell to point of almost to complete inactivity since cells
 Now Do it!

ACTIVITY 3: You explain it.

Directions: Observe and analyze the two graphs, graph A and B, and briefly
explain how the enzyme activity is affected by the factor mentioned in each
graph. Give your interpretation by focusing on the changes of the enzymatic
activity.

Graph A

A. Guide Source: Factors Affecting Enzyme Activity | Easy Biology Class

Question: What is the relationship between temperature and rate of reaction? 71
Take in consideration the optimal rate in the graph. (5 points)
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

Graph B Source:

Factors Affecting Enzyme Activity | Easy

Biology Class

B. Guide Question: What is the relationship between pH and rate of reaction?

Take in consideration the optimal pH in the graph. (5 points)
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________
___________________________________________________________________________

Ace it!

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Evangelista, Luisito T. General Biology 1 for Senior High School. C&E Publishing, Inc., 2017. pp. 26 Fig. 1.33
Directions: Check (/) the box if the concept in each item is a fact, if bluff
mark an X.

1. All enzyme reaction rates can be affected by external factors.

2. Denaturation occurs when enzyme temperature exceeds its capacity.
3. The presence or absence of water as well as its amount do not affect
enzymatic activity.
4. Enzymatic activity will be faster if there is an increase in inhibitors.
5. Increased substrate concentration can affect enzymatic activity by
efficient and faster products formation as there will be more ‘workers’
to do the work.

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BIBLIOGRAPHY
“Factors affecting enzymatic activity.” [PPT].
https://www.easybiologyclass.com/factors-affecting-enzymatic-activity-ppt/
Accessed April 23, 2021

“Factors affecting enzymatic activity.” [pdf].

https://core.ac.uk/download/pdf/10677459.pdf Accessed April 23, 2021

“Factors Affecting Enzyme Activity: Easy Biology Class.” Easy Biology Class | Free
Online Biology Tutorials, Lecture Notes & Videos for Life Science Students &
Teachers, 8 June 2019, www.easybiologyclass.com/factors-affecting-
enzymatic-activity-biochemistry-lecture-notes/

“Factors affecting enzymatic activity notes.” [pdf].

https://www.easybiologyclass.com/wp-content/uploads/2019/06/Factos-
Affecting-Enzymatic-Activity-Notes-PDF.pdf Accessed April 25, 2021

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