See discussions, stats, and author profiles for this publication at: https://www.researchgate.

net/publication/326821409

Effect of thymol and oxalic acid according to application method against

Varroa destructor in Honeybees

Article  in  Journal of Biotechnology · August 2018

DOI: 10.1016/j.jbiotec.2018.06.153

CITATIONS READS

0 80

2 authors:

Nevin Keskin Erkay Ozgor

Hacettepe University Cyprus International University
60 PUBLICATIONS   1,037 CITATIONS    24 PUBLICATIONS   51 CITATIONS   

SEE PROFILE SEE PROFILE

Some of the authors of this publication are also working on these related projects:

Next generation of colorimetric biosensors for diagnostic purposes View project

All content following this page was uploaded by Erkay Ozgor on 03 May 2021.

The user has requested enhancement of the downloaded file.

S48 Abstracts / Journal of Biotechnology 280S (2018) S32–S91
Evaulation of Candida albicans GSY1 gene
promoter as a Cd inducible promoter
Hulya Karaca Gencer 1,∗ , Handan Acelya Kapkac 2
1 D’souza 4 , Mayank Varun 4 , Manoj S. Paul 4
Department of P. Microbiology, Faculty of
Pharmacy, Anadolu University, Eskisehir, Turkey
2 Department of Biology, Faculty of Science, Anadolu
University, Eskisehir, Turkey
E-mail address: hulyakaraca@anadolu.edu.tr (H.K. Gencer).
Glycogen is one of the key metabolites for stress resistance in yeast.
Glycogen synthase 1 GSY1 and glycogen synthase 2 GSY2 take a roll
in synthesising of glycogen. Accumulation of glycogen is important
to cope with the starvation. Metabolism of glycogen was investi-
gated in model organism S. cerevisiae but there is a limited study
on C. albicans. We analysed the glycogen metabolism of C. albicans
under different stress conditions. Among these stress conditions,
Cd stress results was interesting. C. albicans treated with 100 ␮M
CdCI2 for 4 h and RT-PCR results showed that GSY1 of C. albicans
was expressed 78-fold more under cadmium stress comparing with
non-treated cells. Taking into consideration of our results, putative
GSY1 promoter could be used as an inducible promoter in S. cere-
visiae. Almost 800 bp upstream fragment of gene and truncated
form of genes fused with GFP and analysed as a cadmium detec-
tor in S. cerevisiae. Leading the environmental contaminant such
as cadmium is very common. Therefore, fast and easy detection of
this heavy metal is very important. In our study, GSY1 promoter-
GFP fusion protein was cloned into S. cerevisiae. After inducing with
Cd, recombinant cells showed significantly high fluorescence com-
paring with native promoter.
https://doi.org/10.1016/j.jbiotec.2018.06.152
Effect of thymol and oxalic acid according to
application method against Varroa destructor
in Honeybees
Nevin Keskin 1,∗ , Erkay Özgör 2
1 Department of Biology, Faculty of Science,
Hacettepe University, Ankara, Turkey
2 Faculty of Arts and Sciences, Mersin, Turkey
E-mail address: nevink@hacettepe.edu.tr (N. Keskin).
The developmental period of honeybees can provide an oppor-
tunity for harmful pathogens and parasites. One of the pests for
honey bees is Varroa destructor mite which can harm adult bees.
There are many techniques for fight with Varroa. In this study, 3%
oxalic acid and 60% thymol were tested against the Varroa mite in
hives by spraying and dripping methods. 15 hives which had Var-
roa were selected for each treatment. An untreated hive with Varroa
was chosen as a control. The application of these compounds was
carried out twice in both autumn and spring and the quantity of
Varroa was evaluated by examining bee samples from hives. We
found a significant difference between bees treated with both sub-
stances and the control group. The amount of Varroa in the treated
hives decreased significantly. Thymol was found to be more effec-
tive product than oxalic acid. A Spraying method of application
was found to be more effective for both substances. We conclude
that thymol is an effective agent against Varroa. However, it has
been found that the application method is very important for the
effectiveness of the substances.
https://doi.org/10.1016/j.jbiotec.2018.06.153
View publication stats
Biomonitoring of rare earth elements by
aquatic mosses in a mining region
Paulo J.C. Favas 1,2,â'^ , Joao Pratas 2,3 , Rohan
1 School of Life Sciences and the Environment,
University of Tras-os-Montes e Alto Douro, Vila Real,
Portugal
2 MARE ⣓ Marine and Environmental Sciences
Centre, University of Coimbra, Coimbra, Portugal
3 Faculty of Sciences and Technology, University of
Coimbra, Coimbra, Portugal
4 Department of Botany, St. John’s College, Agra, India
E-mail address: pjcf@utad.pt (P.J.C. Favas).
Distribution of 14 rare earth elements (REEs) was studied at 21
sites in streams of Gois mining region in Central Portugal. REEs
or lanthanides are 15 metals divided into two groups based on
their atomic numbers. These are light-rare earths or LREEs (La
to Gd) and heavy-rare earths or HREEs (Tb to Lu). They are usu-
ally found together, though they are difficult to extract and purify
given their low concentrations in ores. Four aquatic mosses (Fonti-
nalis squamosa, Brachythecium rivulare, Platyhypnidium riparioides,
Thamnobryum alopecurum) along with water samples were ana-
lysed by ICP-MS. Thirteen REEs (0.001⣓2.81 μg/L) were detected
in the stream water. Mean content of LREEs was higher than that
of HREEs. Fourteen REEs and two actinides were detected in moss
samples. LREE uptake (0.17⣓12.2 mg/kg) was greater than HREE
uptake (0.02⣓0.78 mg/kg) in all mosses. The uptake potential of
the studied mosses indicates that they can accumulate REEs at
much higher concentrations than those in their ambient water.
Brachythecium rivulare is more suitable for LREEs and T. alopecurum
for HREEs. These species may be suitable for active biomonitoring
Acknowledgements: This study had the support of Fundacao
para a Ciência e Tecnologia (FCT), through the strategic project
UID/MAR/04292/2013 granted to MARE.
https://doi.org/10.1016/j.jbiotec.2018.06.154
Chemically modified bacterial cellulose for
nickel ion adsorption
Konstantin Nikolayevich Konstantinov ∗ , Nikolay
Alexandrovich Pestov, Victor Vasilievich Revin
Department of Biotechnology, Bioengineering and
Biochemistry, National Research Mordovia State
University, Saransk, Russia
E-mail address: kostia1595@yandex.ru (K.N. Konstantinov).
Pollution with heavy metals leads to the development of diseases
and irreversible changes in internal human organs. Heavy metal
removal by adsorption and ion exchange are effective methods of
wastewater treatment. The purpose of current work was to study of
the adsorptive properties of bacterial cellulose in relation to nickel
ions. Bacterial cellulose has a large specific surface area, poros-
ity and a large number of functional groups. Bacterial cellulose
gel films were obtained in static conditions of Gluconacetobacter
sucrofermentans cultivation. This polymer has been characterized
by methods of electronic spectroscopy, the X-ray diffraction analy-
sis, TGA and IR spectroscopy. It was found that unmodified bacterial
cellulose has a low adsorption capacity in relation to nickel ions.
To improve the adsorption capacity of bacterial cellulose a chem-
ical modification was carried out by oxidation in order to obtain
different contents of carboxyl groups. The modified bacterial cel-
lulose adsorbed from water not less than 17 mg of nickel sulphate