ST.

ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com

Name: _______________________________________ Date:__________________

Group No. __________ Score: _________________

Activity No. 6: Stability of Proteins

Objectives
At the end of the experiment, student should be able to:
 Identify the physical and chemical properties of proteins.
 Identify denaturants and describe their effects on proteins.
General Procedure:
Perform the following tests using 10ml solutions of the following as samples:
casein, egg albumin and gelatin. Label samples. In these activity, coagulation
or precipitation will indicate denaturation.
A. Heat Denaturation
1. Heat 2ml of each of the sample in different test tubes then label.
2. Observe if it coagulates.
3. Add 2 drops of 1.0% acetic acid in each test tube and observe the effect.
4. Record the result
Before After

Heat Denaturation Observation

1. Casein
2. Egg albumin
3. Gelatin

B. Isoelectric Precipitation
1. Obtain 2mL of each of the sample in different test tubes then label.
2. Add 0.1N HCl drop by drop until maximum precipitation occurs in each sample.
3. Continue the addition of HCl, shaking the tube after each addition, and note that the proteins
redissolve.
4. At this point, add 0.1N NaOH drop by drop in each test tube. Note whether the protein is
precipitated and redissolved in an excess alkali.

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 ST. ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com

5. Record the result.

Before Result

Isoelectric precipitation Observation

1. Casein
2. Egg albumin
3. Gelatin

C. Concentrated Mineral Acids

1. Obtain 1ml each of the sample in different test tubes then label.
2. Add 2 drops of hydrochloric acid on each sample.
3. Observe the effect
4. Record the result.
Before After

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https://www.coursehero.com/file/111882641/Activity-6-Stability-of-Proteinspdf/
 ST. ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com

Concentrated mineral acids Observation

1. Casein
2. Egg albumin
3. Gelatin

D. Organic Solvents
1. Obtain 2mL of the each of the sample in different test tubes then label.
2. Add 1 drop of 1.0% acetic acid and 4mL of 95% ethyl alcohol in each test tube.
3. Mix thoroughly.
4. Observe the characteristics of the precipitate.

Before After

Organic Solvents Observation

1. Casein
2. Egg albumin

3. Gelatin

This study source was downloaded by 100000811681019 from CourseHero.com on 10-13-2022 08:35:02 GMT -05:00

https://www.coursehero.com/file/111882641/Activity-6-Stability-of-Proteinspdf/
 ST. ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com

Learning Discussions:
1. Surgical instruments are sterilized by heating them, while alcohol is used as disinfectant in
cleansing the skin prior to an infection. Why are these methods successful in killing harmful
microorganisms?

2. Explain why egg whites and milk are used as antidotes for heavy metal poisoning.

3. What is the purpose of alkaloidal reagents?

4. Where is denaturation of proteins important?

This study source was downloaded by 100000811681019 from CourseHero.com on 10-13-2022 08:35:02 GMT -05:00

https://www.coursehero.com/file/111882641/Activity-6-Stability-of-Proteinspdf/
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