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Activity 6 Stability of Proteins PDF
Activity 6 Stability of Proteins PDF
ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com
B. Isoelectric Precipitation
1. Obtain 2mL of each of the sample in different test tubes then label.
2. Add 0.1N HCl drop by drop until maximum precipitation occurs in each sample.
3. Continue the addition of HCl, shaking the tube after each addition, and note that the proteins
redissolve.
4. At this point, add 0.1N NaOH drop by drop in each test tube. Note whether the protein is
precipitated and redissolved in an excess alkali.
This study source was downloaded by 100000811681019 from CourseHero.com on 10-13-2022 08:35:02 GMT -05:00
https://www.coursehero.com/file/111882641/Activity-6-Stability-of-Proteinspdf/
ST. ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com
This study source was downloaded by 100000811681019 from CourseHero.com on 10-13-2022 08:35:02 GMT -05:00
https://www.coursehero.com/file/111882641/Activity-6-Stability-of-Proteinspdf/
ST. ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com
1. Casein
2. Egg albumin
3. Gelatin
D. Organic Solvents
1. Obtain 2mL of the each of the sample in different test tubes then label.
2. Add 1 drop of 1.0% acetic acid and 4mL of 95% ethyl alcohol in each test tube.
3. Mix thoroughly.
4. Observe the characteristics of the precipitate.
Before After
1. Casein
2. Egg albumin
3. Gelatin
This study source was downloaded by 100000811681019 from CourseHero.com on 10-13-2022 08:35:02 GMT -05:00
https://www.coursehero.com/file/111882641/Activity-6-Stability-of-Proteinspdf/
ST. ALEXIUS COLLEGE
Gen. San. Drive, City of Koronadal, South Cotabato, Philippines 09506, Tel.: (083) 228-2019, Fax: (083) 228-4015, Email: st.alexiuscollege@yahoo.com
Learning Discussions:
1. Surgical instruments are sterilized by heating them, while alcohol is used as disinfectant in
cleansing the skin prior to an infection. Why are these methods successful in killing harmful
microorganisms?
2. Explain why egg whites and milk are used as antidotes for heavy metal poisoning.
This study source was downloaded by 100000811681019 from CourseHero.com on 10-13-2022 08:35:02 GMT -05:00
https://www.coursehero.com/file/111882641/Activity-6-Stability-of-Proteinspdf/
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